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Testing the Wildlink activity-detection system on wolves and white-tailed deer

We tested the reliability and predictive capabilities of the activity meter in the new Wildlink Data Acquisition and Recapture System by comparing activity counts with concurrent observations of captive wolf (Canis lupus) and free-ranging white-tailed deer (Odocoileus virginianus) activity. The Wildlink system stores activity data in a computer within a radio collar with which a biologist can communicate. Three levels of activity could be detected. The Wildlink system provided greater activity discrimination and was more reliable, adaptable, and efficient and was easier to use than conventional telemetry activity systems. The Wildlink system could be highly useful for determining wildlife energy budgets.

Canadian Journal of Zoology

Status of a broadly distributed endangered species: Results and implications of the second International Piping Plover Census

Methods for monitoring progress toward recovery goals are highly variable and may be problematic for endangered species that are mobile and widely distributed. Recovery objectives for Piping Plovers ( Charadrius melodus ) include attainment of minimum population sizes within specified recovery units, as determined by two U.S. and two Canadian recovery teams. To assess progress toward these goals, complete surveys of the species' winter and breeding ranges in Canada, the United States, Mexico, the Bahamas, and the Greater Antilles are conducted every 5 years. In 1996, 1200 biologists and volunteers participated in the second International Piping Plover Census, tallying 2515 wintering birds and 5913 adults (2668 breeding pairs) during the breeding census. Winter numbers were 27% lower than those of the first international census conducted in 1991, with substantially fewer wintering birds along the Gulf of Mexico and an overall increase in numbers along the Atlantic Coast. Large numbers of wintering plovers remain undetected. In 1996, the total number of breeding adults was 7.7% higher than in 1991. Regionally, breeding numbers were 31% higher along the Atlantic Coast and 20% higher in the small Great Lakes population, but declined by 5% in the U.S. Great Plains and the Canadian Prairie. Target recovery numbers were met only for Saskatchewan but were approached in Alberta and New England. The results suggest that Piping Plover distribution and habitat use in the U.S. Great Plains/Canadian Prairie region may shift dramatically with water conditions.

Greater Antilles

Prodigious polyphyly in imperilled freshwater pearly-mussels (Bivalvia: Unionidae): a phylogenetic test of species and generic designations

Unionid bivalves or freshwater pearly-mussels (Unionoidea: Unionidae) serve as an exemplary system for examining many of the problems facing systematists and conservation biologists today. Most of the species and genera were described in the late 1800s and early 1900s, but few phylogenetic studies have been conducted to test conventional views of species and classification. Pearly-mussels of Gulf Coastal drainages of the southeastern United States from the Escambia (southern Alabama to Florida) to the Suwannee Rivers (Florida) are a unique fauna comprised of approximately 100 species, with about 30 endemic to the region. In this study, mitochondrial cytochrome c oxidase subunit I and 16S rRNA gene sequences were used to test the monophyly and to estimate evolutionary relationships of five unionid species representing three different genera. The molecular phylogenies depict all three genera as polyphyletic. The prodigious polyphyly exhibited within unionids is due to incorrect notions of homology and false assumptions about missing anatomical data. In contrast, the molecular phylogeny provides evidence to support the recognition of all five unionid species as distinct evolutionary entities. Furthermore, molecular genealogical evidence supports the elevation of Quincuncina infucata (Conrad) of the Suwannee River to species level, for which Q. kleiniana (Lea) is available.

Geological Society Special Publication

Partly cloudy with a chance of migration: Weather, radars, and aeroecology

Aeroecology is an emerging scientific discipline that integrates atmospheric science, Earth science, geography, ecology, computer science, computational biology, and engineering to further the understanding of biological patterns and processes. The unifying concept underlying this new transdisciplinary field of study is a focus on the planetary boundary layer and lower free atmosphere (i.e., the aerosphere), and the diversity of airborne organisms that inhabit and depend on the aerosphere for their existence. Here, we focus on the role of radars and radar networks in aeroecological studies. Radar systems scanning the atmosphere are primarily used to monitor weather conditions and track the location and movements of aircraft. However, radar echoes regularly contain signals from other sources, such as airborne birds, bats, and arthropods. We briefly discuss how radar observations can be and have been used to study a variety of airborne organisms and examine some of the many potential benefits likely to arise from radar aeroecology for meteorological and biological research over a wide range of spatial and temporal scales. Radar systems are becoming increasingly sophisticated with the advent of innovative signal processing and dual-polarimetric capabilities. These capabilities should be better harnessed to promote both meteorological and aeroecological research and to explore the interface between these two broad disciplines. We strongly encourage close collaboration among meteorologists, radar scientists, biologists, and others toward developing radar products that will contribute to a better understanding of airborne fauna.

Bulletin of the American Meteorological Society

Coelomic disorders of fishes

Dropsy is a commonly applied term for coelomic distention due to ascites, or the effusion and collection of fluid freely throughout the coelomic cavity. Dropsy, or ascites, is generally a sign of another ongoing disease process, oftentimes one that is multisystemic and impacting coelomic organs and tissues. Dropsy may be caused by a variety of potential etiological agents, both infectious and noninfectious. Stressors such as rapid change in water temperature may predispose fish to bacterial diseases associated with dropsy. Clinically affected fish display coelomic distension of varying degrees of severity. Generally considered a disease syndrome similar to dropsy, affecting specifically Malawi cichlids, a popular group of ornamental fish among aquarium hobbyists. Water belly occurs among salt water pen-reared salmonid fish, including Atlantic salmon, Chinook salmon and rainbow trout. Dietary changes such as reduction of the feeding rate have been reported to improve the condition among affected fish.

Book chapter

Clutch may predict growth of hatchling Burmese pythons better than food availability or sex

Identifying which environmental and genetic factors affect growth pattern phenotypes can help biologists predict how organisms distribute finite energy resources in response to varying environmental conditions and physiological states. This information may be useful for monitoring and managing populations of cryptic, endangered, and invasive species. Consequently, we assessed the effects of food availability, clutch, and sex on the growth of invasive Burmese pythons ( Python bivittatus Kuhl) from the Greater Everglades Ecosystem in Florida, USA. Though little is known from the wild, Burmese pythons have been physiological model organisms for decades, with most experimental research sourcing individuals from the pet trade. Here, we used 60 hatchlings collected as eggs from the nests of two wild pythons, assigned them to High or Low feeding treatments, and monitored growth and meal consumption for 12 weeks, a period when pythons are thought to grow very rapidly. None of the 30 hatchlings that were offered food prior to their fourth week post-hatching consumed it, presumably because they were relying on internal yolk stores. Although only two clutches were used in the experiment, we found that nearly all phenotypic variation was explained by clutch rather than feeding treatment or sex. Hatchlings from clutch 1 (C1) grew faster and were longer, heavier, in better body condition, ate more frequently, and were bolder than hatchlings from clutch 2 (C2), regardless of food availability. On average, C1 and C2 hatchling snout-vent length (SVL) and weight grew 0.15 cm d −1 and 0.10 cm d −1 , and 0.20 g d −1 and 0.03 g d −1 , respectively. Additional research may be warranted to determine whether these effects remain with larger clutch sample sizes and to identify the underlying mechanisms and fitness implications of this variation to help inform risk assessments and management.

Biology Open

Assessing the geologic and climatic forcing of biodiversity and evolution surrounding the Gulf of California

For almost a century the Baja California peninsula (Peninsula), Gulf of California (Gulf), and broader Sonoran Desert region (figure 1) have drawn geologists and biologists alike to study its unique physical and evolutionary processes (e.g., Wittich 1920; Darton 1921; Nelson 1921; Johnston 1924; Beal 1948; Durham and Allison 1960). The challenge remains to untangle the long, intricate, and at times enigmatic geological and climatological histories that have shaped the high levels of endemism and biodiversity observed in the region today (Van Devender 1990; Grismer 2000; Riddle et al. 2000).

Gulf of California

“One Health” or three? Publication silos among the One Health disciplines

The One Health initiative is a global effort fostering interdisciplinary collaborations to address challenges in human, animal, and environmental health. While One Health has received considerable press, its benefits remain unclear because its effects have not been quantitatively described. We systematically surveyed the published literature and used social network analysis to measure interdisciplinarity in One Health studies constructing dynamic pathogen transmission models. The number of publications fulfilling our search criteria increased by 14.6% per year, which is faster than growth rates for life sciences as a whole and for most biology subdisciplines. Surveyed publications clustered into three communities: one used by ecologists, one used by veterinarians, and a third diverse-authorship community used by population biologists, mathematicians, epidemiologists, and experts in human health. Overlap between these communities increased through time in terms of author number, diversity of co-author affiliations, and diversity of citations. However, communities continue to differ in the systems studied, questions asked, and methods employed. While the infectious disease research community has made significant progress toward integrating its participating disciplines, some segregation—especially along the veterinary/ecological research interface—remains.

PLoS Biology

An accessible method for implementing hierarchical models with spatio-temporal abundance data

A common goal in ecology and wildlife management is to determine the causes of variation in population dynamics over long periods of time and across large spatial scales. Many assumptions must nevertheless be overcome to make appropriate inference about spatio-temporal variation in population dynamics, such as autocorrelation among data points, excess zeros, and observation error in count data. To address these issues, many scientists and statisticians have recommended the use of Bayesian hierarchical models. Unfortunately, hierarchical statistical models remain somewhat difficult to use because of the necessary quantitative background needed to implement them, or because of the computational demands of using Markov Chain Monte Carlo algorithms to estimate parameters. Fortunately, new tools have recently been developed that make it more feasible for wildlife biologists to fit sophisticated hierarchical Bayesian models (i.e., Integrated Nested Laplace Approximation, ‘INLA’). We present a case study using two important game species in North America, the lesser and greater scaup, to demonstrate how INLA can be used to estimate the parameters in a hierarchical model that decouples observation error from process variation, and accounts for unknown sources of excess zeros as well as spatial and temporal dependence in the data. Ultimately, our goal was to make unbiased inference about spatial variation in population trends over time.

PLoS ONE

Concealment of juvenile bull trout in response to temperature, light, and substrate: Implications for detection

Bull trout ( Salvelinus confluentus) are challenging to detect as a result of the species cryptic behavior and coloration, relatively low densities in complex habitats, and affinity for cold, high clarity, low conductivity waters. Bull trout are also closely associated with the stream bed, frequently conceal in substrate, and this concealment behavior is poorly understood. Consequently, population assessments are problematic and biologists and managers often lack quantitative information to accurately describe bull trout distributions, estimate abundance, and assess status and trends; particularly for stream-dwelling populations. During controlled laboratory trials, we recorded concealment, resting, and swimming behavior of juvenile wild bull trout in response to: (1) constant and fluctuating water temperature, (2) presence or absence of light, and (3) substrate size. Light level had the strongest influence on wild fish concealment and more fish concealed as light levels increased from darkness to daylight. Wild fish were 14.5 times less likely to conceal in constant darkness and 4.1 times more likely to conceal in 12 h light x 12 h darkness compared to constant light. Wild fish were 6.2 times less likely to conceal in small (26–51 mm) substrate compared to larger (52–102 mm) substrate. As water temperature increased, fewer wild fish concealed. Knowledge of wild bull trout concealment will improve field sampling protocols and increase detection efficiencies. These data also enhance knowledge of bull trout niche requirements which illuminates ecological differences among species and informs conservation and restoration efforts.

PLoS ONE

Assembling a safe and effective toolbox for integrated flea control and plague mitigation: Fipronil experiments with prairie dogs

Background Plague, a widely distributed zoonotic disease of mammalian hosts and flea vectors, poses a significant risk to ecosystems throughout much of Earth. Conservation biologists use insecticides for flea control and plague mitigation. Here, we evaluate the use of an insecticide grain bait, laced with 0.005% fipronil (FIP) by weight, with black-tailed prairie dogs (BTPDs, Cynomys ludovicianus ). We consider safety measures, flea control, BTPD body condition, BTPD survival, efficacy of plague mitigation, and the speed of FIP grain application vs. infusing BTPD burrows with insecticide dusts. We also explore conservation implications for endangered black-footed ferrets ( Mustela nigripes ), which are specialized predators of Cynomys . Principal findings During 5- and 10-day laboratory trials in Colorado, USA, 2016–2017, FIP grain had no detectable acute toxic effect on 20 BTPDs that readily consumed the grain. During field experiments in South Dakota, USA, 2016–2020, FIP grain suppressed fleas on BTPDs for at least 12 months and up to 24 months in many cases; short-term flea control on a few sites was poor for unknown reasons. In an area of South Dakota where plague circulation appeared low or absent, FIP grain had no detectable effect, positive or negative, on BTPD survival. Experimental results suggest FIP grain may have improved BTPD body condition (mass:foot) and reproduction (juveniles:adults). During a 2019 plague epizootic in Colorado, BTPDs on 238 ha habitat were protected by FIP grain, whereas BTPDs were nearly eliminated on non-treated habitat. Applications of FIP grain were 2–4 times faster than dusting BTPD burrows. Significance Deltamethrin dust is the most commonly used insecticide for plague mitigation on Cynomys colonies. Fleas on BTPD colonies exhibit the ability to evolve resistance to deltamethrin after repeated annual treatments. Thus, more tools are needed. Accumulating data show orally-delivered FIP is safe and usually effective for flea control with BTPDs, though potential acute toxic effects cannot be ruled out. With continued study and refinement, FIP might be used in rotation with, or even replace deltamethrin, and serve an important role in Cynomys and black-footed ferret conservation. More broadly, our stepwise approach to research on FIP may function as a template or guide for evaluations of insecticides in the context of wildlife conservation.

South Dakota

Aquatic invasive species: Lessons from cancer research

Aquatic invasive species are disrupting ecosystems with increasing frequency. Successful control of these invasions has been rare: Biologists and managers have few tools for fighting aquatic invaders. In contrast, the medical community has long worked to develop tools for preventing and fighting cancer. Its successes are marked by a coordinated research approach with multiple steps: prevention, early detection, diagnosis, treatment options and rehabilitation. The authors discuss how these steps can be applied to aquatic invasive species, such as the American bullfrog (Lithobates catesbeianus), in the Northern Rocky Mountain region of the United States, to expedite tool development and implementation along with achievement of biodiversity conservation goals.

American Scientist

Time-specific patterns of nest survival for ducks and passerines breeding in North Dakota

In many bird species, survival can vary with the age of the nest, with the date a nest was initiated, or among years within the same nesting area. A literature review showed that patterns of survival vary in relation to nest age and date and are often contradictory. Inconsistencies could be a result of temporal variation in the environment or life-history differences among species. We examined patterns of nest survival in relation to nest age, date, and year for several duck and passerine species nesting at a single location in North Dakota during 1998–2003. We predicted that if environment shaped nest survival patterns, then temporal patterns in survival might be similar among three species of upland nesting ducks, and also among three species of grassland passerines nesting at the same site. We expected that survival patterns would differ between ducks and passerines because of relatively disparate life histories and differences in predators that prey on their nests. Nest survival was rarely constant among years, seasonally, or with age of the nest for species that we studied. As predicted, the pattern of survival was similar among duck species, driven mainly by differences in nest survival associated with nest initiation date. The pattern of survival also was similar among passerine species, but nest survival was more influenced by nest age than by date. Our findings suggest that some but not all variation in temporal patterns of nest survival in grassland birds reported in the literature can be explained on the basis of temporal environmental variation. Because patterns of survival were dissimilar among ducks and passerines, it is likely that mechanisms such as predation or brood parasitism have variable influences on productivity of ducks and passerines nesting in the same area. Our results indicate that biologists and managers should not assume that temporal environmental variations, especially factors that affect nest survival, act similarly on all grassland birds.

North Dakota

The Geomyces fungi: ecology and distribution

White-nose syndrome (WNS) is a devastating disease affecting hibernating bats, first documented in winter 2006 in eastern North America. Over 5.5 million bats of several species may have died as a result of this disease. The fungus Geomyces destructans is now considered the causal agent of WNS, and this species may have been recently introduced into North American bat hibernation habitats. This overview summarizes the ecology and distribution of Geomyces fungi. Species in this genus are common in the soils of temperate and high-latitude ecosystems and are capable of withstanding and thriving in cold, low-nutrient polar environments. These species are dispersed by wind, groundwater, arthropods, birds, and mammals and are carried by humans, their clothing, and their equipment. These characteristics present significant challenges to biologists, managers, and others charged with controlling the spread of WNS and G. destructans in other parts of North America and the biosphere.

BioScience

Adventures on the roof of the world

T o conduct field biology requires tenacity, grit, and flexibility; to endeavor to achieve conservation success requires patience, persistence, and passion. The essence of field biology and the hope for conservation success are both reflected admirably in George B. Schaller's most recent book, Tibet Wild: A Naturalist's Journeys on the Roof of the World . I can think of no living biologist who embodies these characteristics more than Schaller does. Nearly 80 years old, he still regularly treks in faraway lands, observing and recording the natural history of species that the vast majority of us will never see in the wild. Schaller is a vanguard, and Tibet Wild , like his other books, is a sentinel of urgent conservation need.

BioScience

An evaluation of multipass electrofishing for estimating the abundance of stream-dwelling salmonids

Failure to estimate capture efficiency, defined as the probability of capturing individual fish, can introduce a systematic error or bias into estimates of fish abundance. We evaluated the efficacy of multipass electrofishing removal methods for estimating fish abundance by comparing estimates of capture efficiency from multipass removal estimates to capture efficiencies measured by the recapture of known numbers of marked individuals for bull trout Salvelinus confluentus and westslope cutthroat trout Oncorhynchus clarki lewisi. Electrofishing capture efficiency measured by the recapture of marked fish was greatest for westslope cutthroat trout and for the largest size-classes of both species. Capture efficiency measured by the recapture of marked fish also was low for the first electrofishing pass (mean, 28%) and decreased considerably (mean, 1.71 times lower) with successive passes, which suggested that fish were responding to the electrofishing procedures. On average, the removal methods overestimated three-pass capture efficiency by 39% and under-estimated fish abundance by 88%, across both species and all size-classes. The overestimates of efficiency were positively related to the cross-sectional area of the stream and the amount of undercut banks and negatively related to the number of removal passes for bull trout, whereas for westslope cutthroat trout, the overestimates were positively related to the amount of cobble substrate. Three-pass capture efficiency measured by the recapture of marked fish was related to the same stream habitat characteristics that influenced (biased) the removal estimates and did not appear to be influenced by our sampling procedures, including fish marking. Simulation modeling confirmed our field observations and indicated that underestimates of fish abundance by the removal method were negatively related to first-pass sampling efficiency and the magnitude of the decrease in capture efficiency with successive passes. Our results, and those of other researchers, suggest that most electrofishing-removal-based estimates of fish abundance are likely to be biased and that these biases are related to stream characteristics, fish species, and size. We suggest that biologists regard electrofishing-removal- based estimates as biased indices and encourage them to measure and model the efficiency of their sampling methods to avoid introducing systematic errors into their data.

Transactions of the American Fisheries Society

Formalin treatments pass new tests. Additional notes on the control of ecto-parasitic protozoa

After the completion of the eхреriments reported recently, in which the efficacy of formalin in controlling infections of Gostia mecatr ix was demonstrated, the author was afforded an opportunity to test the value of formalin solutions in combatting established mixed infections of ( Gyrodactylus, Tricbodina, Cyclochaeta ) and a stalked protozoan on rainbow trout fingerlings. This opportunity was provided through the courtesy and cooperation of Clarence F. Pautzke, Chief Biologist for the Washington State Game Department, and Lee Walters, Superintendent of the Washington State Hatchery at Seward Park, Seattle.

Progressive Fish-Culturist

Removal of excess nitrogen in a hatchery water supply

The water system at the U. S. Fish Cultural Station, Leavenworth, Washington, has been supplemented with two wells that were to be used to increase the temperature of the water during the winter and to cool the Water in the summer if necessary. The well water proved to be unsuitable for hatchery purposes because it was supersaturated with nitrogen, causing "gas-bubble" disease among fish subjected to 11. Mr. R. E. Burrows, the district biologist at the Leavenworth laboratory, devised a system by which the water from one well could be used satisfactorily in the hatchery after a circuitous routing through a mixing chamber with considerable agitation and a settling basin. The circuitous routing precluded the use of the rearing ponds, and it did not sufficiently reduce the nitrogen tension of the water from the other well.

Progressive Fish-Culturist