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Effects of a low-lipid diet on the gut microbiome and head kidney transcriptome of juvenile Chinook Salmon

Objective Pacific salmon Oncorhynchus spp. reared in production hatcheries are typically fed high-lipid, energy-dense diets to achieve large size and high body condition prior to release. In contrast, juveniles in natural environments tend to consume low-lipid, high-protein diets, and fish reared for research or conservation purposes are sometimes fed diets that are formulated to mimic natural diets and promote wild-like phenotypes. Understanding how these alternative diets affect fish health beyond growth and body condition could ultimately contribute to improving hatchery fish fitness. Methods In this work, we evaluated changes in the fecal microbiome and gene expression of juvenile Chinook Salmon O. tshawytscha on a standard high-lipid hatchery diet versus a low-lipid diet formulated to mimic the nutrition profile of natural-origin fish. To evaluate the time scale at which diet alters the fecal microbiome, we collected longitudinal samples over a 12-week period and switched the diets of a subset of fish twice during the experiment. We used 16S ribosomal RNA gene amplicon sequencing to characterize fecal microbiome differences between fish on the two diets as well as hatchery-reared fish at a production hatchery, hatchery fish that had been captured after release into a stream, and natural-origin, stream-reared fish of similar ages. Additionally, we conducted RNA sequencing on head kidney samples from laboratory-reared fish to evaluate changes in gene expression in this important immune organ. Results We found that the low-lipid diet and the hatchery diet resulted in microbiomes that differed from the microbiome of natural-origin fish and from each other and that diet-driven changes to the microbiome could occur in under 14 d. The low-lipid diet did not result in a microbiome that resembled the microbiome of naturally produced fish. Instead, the low-lipid diet resulted in a microbiome community that was distinct from those of fish reared on the hatchery diet and fish sampled from the wild. The RNA sequencing results indicated differential enrichment of pathways related to immunity, metabolism, and hormone synthesis between fish that were fed the two experimental diets. Conclusions The results suggest that additional environmental factors influence the microbiome more strongly than diet formulation or that the low-lipid diet has a smaller effect on the microbiome than a natural, ­invertebrate-based diet. Given that the gut microbiome and systemic immune function contribute significantly to disease resistance, our findings highlight the importance of understanding how diets fed to fish in captivity may affect fish health beyond growth and body condition metrics.

Journal of Aquatic Animal Health

Using the horizontal-to-vertical spectral ratio method to estimate thickness of the Barry Arm landslide, Prince William Sound, Alaska

Conducting detailed investigations of large landslides is difficult, especially in the subsurface, largely due to environmental factors such as steep slopes, difficult access, and numerous objective hazards. These factors have made it challenging to accurately estimate the depth to the failure surface of the Barry Arm landslide, a large (roughly 10 8 cubic meters), deep-seated bedrock landslide in Prince William Sound, Alaska, recognized in 2019. The landslide has exhibited accelerated movement in recent years and poses a potential tsunamigenic hazard if rapid failure occurs. Failure surface depth, equivalent to landslide thickness, is a necessary metric for landslide-volume calculations and associated tsunami wave models. In this report, we used seismic noise recorded by a seismometer located on the Barry Arm landslide in Alaska to calculate the horizontal-to-vertical spectral ratio (HVSR) to investigate the site fundamental frequency ( f 0 ) and depth of the failure surface. To ensure that observed peak frequencies in the spectral ratio were related to the underlying stratigraphy (and not caused by other noise sources like nearby glaciers, topographic resonance, weather, or human activities), we also calculated HVSRs using earthquake signals, HVSRs at other seismic stations within a 2.5-kilometer radius, and a standard spectral ratio between the landslide station and other sites. We observed multiple peaks in the landslide HVSR curves at 1.5 hertz (Hz), 4–5 Hz, and 7–11 Hz. The frequencies of these peaks were consistent at the landslide site through time and across methods and were dissimilar to those identified at other seismic stations in the area, making it unlikely the peaks were caused by local noise. Directional HVSRs calculated at 15-degree intervals showed amplification of the higher frequency peaks in the direction parallel to slip, indicating two-dimensional site effects. We used the distinct frequency peaks in the seismic record to develop a 4-layer conceptual model of the landslide wherein the top of the deepest layer represents the primary failure surface, or the boundary between damaged (mobile) and undamaged material. We inverted Rayleigh wave ellipticity curves within this 4-layer configuration with constraints on S-wave velocity and layer thickness based on analogous material properties identified in the literature. This was necessary absent any site-specific subsurface S-wave velocity data. The best-fitting models indicate a mean slope-normal depth to the failure surface of 188 (±9) meters (m), with additional stratigraphic boundaries at 4 and 20 m below ground surface, potentially representing layered motion. These results agree with and improve upon ranges estimated by previous studies and can support future modeling and assessment efforts at Barry Arm.

Alaska

Hyperspectral narrowband imaging spectroscopy: A new paradigm for Earth observation

This editorial introduces the Special Issue entitled “Hyperspectral Narrowband Imaging Spectroscopy: A New Paradigm for Earth Observation” in the August 2026 issue of Photogrammetric Engineering & Remote Sensing (PE&RS), the flagship journal of the American Society for Photogrammetry and Remote Sensing (ASPRS). This volume represents the fourth dedicated hyperspectral special issue published in PE&RS, following earlier contributions by Thenkabail et al. (2025, 2024a, 2024b), and continues ASPRS’s commitment to advancing cutting‑edge imaging spectroscopy research and its applications across Earth system science. Remote sensing is undergoing one of the most profound transformations in its history. The emergence of hyperspectral narrowband (HNB) imaging spectroscopy data, capable of acquiring hundreds of contiguous, narrow spectral bands, has shifted the discipline from observing Earth in a handful of broad spectral windows to capturing continuous spectral signatures of the Planet. This transition marks a decisive break from the multispectral paradigm that has dominated satellite remote sensing for nearly five decades, driven by the advent of new orbital imaging spectrometers such as EnMAP, PRISMA, and NASA’s EMIT, and by the forthcoming Surface Biology and Geology (SBG) mission ((Pires Silva et al., 2026; Bourriz et al., 2025; Thenkabail et al., 2025; Chabrillat et al., 2024; Aneece et al., 2024; Dave et al., 2024; Thenkabail et al., 2024a; Thenkabail et al., 2024b; Thenkabail, 2024a; Thenkabail, 2024b; Thompson et al., 2022; Kokaly et al., 2022; Aneece & Thenkabail, 2022; Cawse Nicholson et al., 2021; Guanter et al., 2021; Vangi et al., 2021; Thenkabail et al., 2021). These missions (e.g., Table 1) deliver unprecedented spectral fidelity, improved signal to noise ratios, and global coverage capabilities, enabling a new era of quantitative, spectroscopy based Earth observation. Where multispectral broadbands (MBBs) provide only a few discrete measurements along the electromagnetic spectrum, HNB systems deliver rich, diagnostic information that enables scientists to characterize Earth’s surface with unprecedented biochemical, biophysical, and structural detail (Figure 1a, 1b). The implications for environmental monitoring, agriculture, water resources, and mineral exploration are profound. Several overarching themes emerge: • Spectral fidelity matters. The ability to preserve subtle absorption features is essential for mineral mapping, vegetation trait retrieval, and biochemical modeling. • AI and deep learning are indispensable. From destriping to classification, modern analytics must be scalable, label‑efficient, and capable of exploiting the full spectral–spatial richness of HNB data. • Physics‑based and data‑driven approaches must converge. Radiative transfer models such as PROSAIL, enhanced with localized soil parameterizations, remain foundational for biophysical retrievals and model‑based inference. • Dimensionality reduction and feature extraction are critical. Techniques such as L1‑ISOMAP demonstrate that intelligent manifold learning can unlock the structure of fused, high‑dimensional datasets. • Next‑generation architectures must be interactive and multimodal. ICTNet exemplifies the future of hyperspectral classification: hybrid, synergistic, and capable of modeling both local textures and global spectral dependencies.

Photogrammetric Engineering and Remote Sensing (PE

Estimated hydrogeologic, spatial, and temporal distribution of self-supplied domestic groundwater withdrawals for aquifers of the Virginia Coastal Plain

Water use from private-domestic wells accounts for nearly 40 percent of total groundwater withdrawals in the Virginia Coastal Plain Physiographic Province (henceforth called the Virginia Coastal Plain). However, because self-supplied domestic water use generally falls below the Virginia Department of Environmental Quality (VDEQ) reporting and management threshold of 300,000 gallons per month, quantifying these withdrawals is challenging. This report builds upon the foundation of previous U.S. Geological Survey investigations by providing revised techniques to improve estimates of the aquifer source, spatial distribution, and monthly magnitude of these groundwater withdrawals. The aquifer sources of private-domestic wells in the Virginia Coastal Plain were estimated by cross-referencing 8,264 well records from the VDEQ and the Virginia Department of Health to a digital model of the Virginia Coastal Plain hydrogeologic framework. This analysis highlights the regional importance of the Yorktown-Eastover, Potomac, and surficial aquifers. Collectively, these three aquifers account for 80 percent of self-supplied domestic groundwater withdrawals. The population using self-supplied domestic water was estimated using census blocks, well-use ratios, building footprints, and land-use and land-cover data to produce a high-resolution, disaggregated, raster-based dataset. This approach improves upon previous models at the census-block or road-network scale by reducing the low-density spread of the self-supplied domestic population across undeveloped areas and concentrating the population and its corresponding water use in the areas where it is most likely to occur. Results show that an estimated 475,332 people comprise the 2020 self-supplied domestic population of the Virginia Coastal Plain, an increase of 5.7 percent since 2010, and the greatest concentrations of self-supplied domestic population surround large cities. Estimates could be further refined with the addition of current and complete spatial data on public water-system service areas. The quantity of water used by the self-supplied domestic population was estimated by modifying published state per-capita water-use coefficients with the corresponding monthly variability assessed from Virginia Coastal Plain public water-system withdrawal data. This analysis estimates an average increase of 12 percent from June through August and an average decrease of 8 percent from December through March from the baseline annual average of 80 gallons per day per capita, which generally matches similar studies in the eastern United States. The application of these revised methodologies for the estimation of private-domestic wells and the self-supplied domestic population improves understanding of domestic groundwater use in the Virginia Coastal Plain across hydrogeologic, spatial, and temporal scales. These revisions help better inform water-resource managers and decision makers and support higher resolution groundwater modeling. Furthermore, these methods are transferrable to other areas where self-supplied domestic water withdrawals are important to the overall water budget.

Virginia

Spatiotemporal variability of algal biomass and nitrate in Owasco and Seneca Lakes in the Finger Lakes Region, New York, in 2019

Cyanobacterial harmful algal blooms (CyanoHABs) have become increasingly common, threatening the security of water resources globally. The U.S. Geological Survey conducted high-resolution nearshore mapping surveys using boat-mounted multiparameter sondes and nitrate sensors during the summer and fall of 2019 on Owasco Lake and Seneca Lake, two lakes with documented CyanoHABs in the Finger Lakes region of New York State. Discrete sensor measurements and water-quality samples were collected at fixed points along survey routes and continuous data were generated at open-water monitoring platforms. This investigation examined whether water-quality information from nearshore surveys was representative of open-water conditions and if nearshore surveys could be used to identify areas with localized nearshore CyanoHABs and potential sources of nutrients not captured by tributary sampling. In addition to comparisons across methods, nearshore concentrations of nitrate and chlorophyll were evaluated relative to tributary outlets, cyanobacterial abundance and biovolume at discrete locations, and the locations of near-surface CyanoHABs that were designated as “confirmed with high toxins” by the New York State Department of Environmental Conservation. Nitrate and chlorophyll concentrations were comparable across methods for each lake, although concentration ranges were typically higher for nearshore mapping datasets than for nearshore discrete datasets. Nearshore surveys indicated areas of nitrate enrichment that varied temporally in both lakes. Orthophosphate was not routinely detected. Across methods, median chlorophyll concentrations were higher for the summer survey than for the fall survey in Owasco Lake. Nearshore chlorophyll concentrations varied more temporally in Owasco Lake than in Seneca Lake. Phytoplankton and cyanobacterial abundance and biovolume were about five times higher in Owasco Lake than in Seneca Lake. Cyanobacteria dominated the phytoplankton community in most samples, and Microcystis comprised the bulk of the cyanobacterial biovolume. The most abundant potential cyanotoxin-producing (specifically microcystins) genera were Microcystis , Synechococcus , Aphanocapsa , and Pseudanabaena . The cyanobacterial community composition was comparable between open-water monitoring platforms and nearshore samples. Microcystins were detected in seven survey samples from Owasco Lake, in one survey sample from Seneca Lake, and in one sample each from the open-water monitoring platforms on Owasco and Seneca Lakes that were collected about 7 days before the fall surveys. Microcystin detections were not consistently associated with high cyanobacterial cell counts or cyanotoxin-producing genera. Results from nearshore surveys were comparable to open-water monitoring platforms and discrete nearshore observations in the absence of nearshore or open-water CyanoHABs in these systems during the study. Patterns of nearshore concentrations of nitrate and chlorophyll from nearshore surveys may aid in the identification of areas with localized nitrate loading and shifts in phytoplankton abundance and community composition.

New York

The EnMAP spaceborne imaging spectroscopy mission: Initial scientific results two years after launch

Imaging spectroscopy has been a recognized and established remote sensing technology since the 1980s, mainly using airborne and field-based platforms to identify and quantify key bio- and geo-chemical surface and atmospheric compounds, based on characteristic spectral reflectance features in the visible-near infrared (VNIR) and short-wave infrared (SWIR). Spaceborne missions, a leap in technology, were sparse, starting with the CHRIS/PROBA and EO1/Hyperion missions in the early 2000s, and providing spectroscopy data with limited spectral coverage and/or low data quality in the SWIR. Since 2019, several countries and agencies have successfully launched a number of spaceborne imaging spectroscopy systems into orbit or deployed them on the International Space Station (ISS) such as DESIS, PRISMA, HISUI, GF-5, EnMAP and EMIT. Among these recent missions, the German Environmental Mapping and Analysis Program (EnMAP) stands for its long-term development, sophisticated design with on-board calibration, high data quality requirements, and extensive accompanying science program. EnMAP was launched in April 2022 and, following a successful commissioning phase, started its operational activities in November 2022. The EnMAP mission encompasses global coverage from 80° N to 80° S through on-demand data acquisitions. Data are free and open access with 30 m spatial resolution, a high spectral resolution with a spectral sampling distance of 6.5 nm and 10 nm in the VNIR and SWIR regions respectively, and a high signal-to-noise ratio. In this paper, we aim to present the mission's current status, coverage, science capabilities and performance two years after launch. We show the potential of EnMAP for space-based imaging spectroscopy to operate in various environments, including high and low light levels, dense forests, Antarctic glaciers, and arid agricultural areas. EnMAP enables various applications in fields such as agriculture and forestry, soil compositional, raw materials, and methane mapping, as well as water quality assessment, and snow and ice properties. The results show that EnMAP's performance exceeds the mission requirements, and highlights the significant potential for contribution to scientific exploitation in various geo- and biochemical sciences. EnMAP is also expected to serve as a key tool for the development and testing of data processing algorithms for upcoming global operational missions.

Remote Sensing of Environment

Investigation of drivers and sources of potential harmful algal blooms in Lake Anna, Virginia, 2023-24

Algal blooms in freshwater reservoirs are an increasing concern because of the risk to aquatic ecosystem health, recreational use, and water quality. Among the taxa that comprise an algal bloom, cyanobacteria are of particular concern due to the potential to produce toxic compounds which can cause acute and chronic illness in humans if ingested through contaminated water or shellfish. When toxins are above a toxin-specific threshold in the water column, the Commonwealth of Virginia categorizes the bloom as a harmful algal bloom (HAB), distinguishing it from an algal bloom without toxins present. Toxins are of particular concern in bodies of water which serve as major recreational areas, such as Lake Anna in central Virginia. Lake Anna is a 27 km-long and 47 km2 reservoir in central Virginia. Since the Commonwealth of Virginia revised the HAB monitoring framework in 2018, algal bloom advisories for potentially toxigenic blooms have been issued in the western, riverine zone of the reservoir. There were detections of microcystin and anatoxin production but no detections of cylindrospermopsin and saxitoxin production in Lake Anna; however, toxin concentrations were low, at values less than 0.65 µg/L. In 2023, the U.S. Geological Survey, in cooperation with Virginia Department of Environmental Quality, initiated a 19-month intensive and extensive study of western Lake Anna and its tributaries (North Anna River and Pamunkey Creek) to (1) characterize the algal community, biomass, and toxin production; (2) identify potential drivers leading to algal bloom initiation, persistence, and decline; and (3) assess the primary sources of the drivers contributing to algal blooms. Continuous and discrete monitoring of water quality within the lake, watershed inputs, meteorological conditions, and algal communities were monitored. Multiple modeling scenarios identified that the formation and persistence of algal blooms in Lake Anna are driven by interactions among chemical, macro- and micronutrient, and physical factors. Key drivers include total nitrogen, total phosphorus, water temperature, suspended sediment concentration, wind speed, copper, iron, molybdenum, nickel, sodium, and alkalinity. During this study, winter stormflow events delivered the highest loading of macro- and micronutrients from the tributaries to the lake. The lake is a sink for these nutrients in the water column or in the lake-bed sediments. During high-occupancy and recreation periods on the lake in the summer months, boating activity can promote internal loading by the resuspension of the lake-bed sediments, thereby increasing the nutrient availability for algal uptake. Thermal stratification and hypoxic conditions were observed, which can contribute to the passive release of nutrients into the water column, further facilitating algal bloom proliferation. These processes may create a positive feedback loop that supports the formation, persistence, and annual recurrence of algal blooms in the western region of Lake Anna. Continued water-quality monitoring of early warning indicators may improve the detection and forecasting of algal growth in the lake and help managers proactively manage water quality and algal growth.

EarthArXiv

Streamflow, ground-water recharge and discharge, and characteristics of surficial deposits in Buzzards Bay Basin, southeastern Massachusetts

Streamflow measurements at 14 low-flow partial-record stations and two discontinued streamflow-gaging stations were related to concurrent streamflows at six long-term streamflow-gaging stations to estimate streamflows at selected flow durations from 50 to 99 percent for water years 1967-91. At low flows, stream discharge per square mile generally increased with increasing percentage of subbasin underlain by stratified-drift deposits. At the 70-, 90-, and 99-percent flow durations, subbasins underlain primarily by stratified-drift deposits had stream discharges per square mile at least 2, 4, and 8 times greater, respectively, than subbasins underlain primarily by till and bedrock deposits. Streamflow measured three times at the Paskamanset River showed that streamflows downstream of municipal pumped wells increased at a lower rate per unit drainage area than streamflows upstream of the pumped wells. When pumpage from wells near the river was added to measured streamflow at the most downstream station, the sum was similar to the potential streamflow at the station if its streamflow per unit area was the same as that upstream from the pumped wells. Mean ground-water recharge and discharge rates were computed from continuous records of daily mean discharge during water years 1967-91 for six streamflow-gaging stations in southeastern Massachusetts and Rhode Island. Estimates of mean ground-water recharge were 19.7 to 22.6 inches per year for stations with drainage areas primarily underlain by till and bedrock deposits, and 23.8 to 25.2 inches per year for stations with drainage areas primarily underlain by stratified-drift deposits. During drought years, annual ground-water recharge can be less than 50 percent of the mean ground-water recharge rate for water years 1967-91. Estimates of ground-water discharge rates generally were 1 to 4 inches per year less than estimates of ground-water recharge rates. Ground-water discharge-duration curves were calculated from hydrograph separation of streamflow records at the six continuous streamflow-gaging stations for water years 1967-91. When streamflows were less than the median streamflow (50-percent duration), ground-water discharges averaged 85.7 percent of the total streamflow at the six streamflow-gaging stations. Ground-water discharges were estimated at the 14 low-flow partial-record stations and two discontinued streamflow-gaging stations by multiplying streamflows at the selected flow durations from 50 to 99 percent for water years 1967-91 by 85.7 percent. Data on surficial deposits in the basin were obtained by drilling 10 wells, by conducting 12 seismic-refraction surveys, and by collecting well log data for 313 wells from consulting firms and the Massachusetts Department of Environmental Management. These data indicated that a few minor corrections could be made to existing saturated thickness maps for stratified-drift deposits in Buzzards Bay Basin. Few well logs were detailed enough to estimate transmissivities; thus existing transmissivity maps were not updated for stratified-drift deposits in the basin.

Massachusetts

Quality of groundwater used for domestic supply in the eastern Sacramento Valley and adjacent foothills, California

Summary More than 2 million Californians rely on groundwater from privately owned domestic wells for drinking-water supply. This report summarizes a water-quality survey of domestic and small-system drinking-water supply wells in the eastern Sacramento Valley and adjacent foothills where more than 25,000 residents are estimated to use privately owned domestic wells. Study results show that inorganic and organic constituents in groundwater were present above regulatory (maximum contaminant level, MCL) benchmarks for public drinking-water quality in 8 and 3 percent, respectively, of the aquifer area used for domestic drinking-water supply (herein, “domestic groundwater resources”; fig. 1). The only inorganic constituent detected above regulatory benchmarks was arsenic. The only organic constituent exceeding regulatory benchmarks was the fumigant 1,2,3-trichloropropane (1,2,3-TCP). Three additional organic constituents—the disinfection by-product chloroform, the gasoline oxygenate methyl tert -butyl ether (MTBE), and the solvent tetrachloroethene (PCE)—were detected at low concentrations below one-tenth of regulatory benchmarks in 34, 10, and 10 percent of domestic groundwater resources, respectively. Total dissolved solids (TDS), iron, and manganese exceeded non-regulatory aesthetic guidelines for drinking water in 5, 10, and 26 percent of domestic groundwater resources, respectively. Per- and polyfluoroalkyl substances (PFASs) were detected in 29 percent of domestic groundwater resources,with 5 percent exceeding the recently enacted (April 2024) U.S. Environmental Protection Agency MCLs. Total coliform and enterococci bacteria were detected in 13 and 8 percent of domestic groundwater resources, respectively. Redox sensitive constituents in this study included arsenic, manganese, nitrate, and iron. In the lower elevation portions of the eastern Sacramento Valley study area, reducing conditions in groundwater aquifers promote elevated arsenic, iron, and manganese, and conversely lower concentrations of nitrate. The presence of the volatile organic compound (VOC) 1,2,3-TCP was related to its past history in select agricultural land uses (on orchards or vineyards) in the Sacramento Valley; however, unlike in the San Joaquin Valley where orchards and vineyards are more common, its detection frequency was low (only detected in one well in this study). Chloroform was frequently detected in this study at low levels. Chloroform is a disinfection byproduct commonly found in domestic wells treated by shock chlorination. The solvent PCE is among the most frequently detected VOCs in groundwater, which is primarily related to its long history of use and its persistence in groundwater in oxic conditions. The gasoline oxygenate MTBE was a contaminant introduced to groundwater through atmospheric exchange when it was used as a fuel additive to decrease smog inducing emissions from vehicles. Its occurrence in groundwater at low levels is expected and makes it a potentially useful tracer of relatively recent recharge water being withdrawn from wells. The PFASs are anthropogenic chemicals with hundreds of uses, and they have been incorporated into many different products, processes, and applications worldwide. Like MTBE, the occurrence of PFASs in groundwater may be in part due to atmospheric exchange, but there are several other pathways that contribute PFASs to the environment.

California

Cancer risk and estimated lithium exposure in drinking groundwater in the US

Importance Lithium is a naturally occurring element in drinking water and is commonly used as a mood-stabilizing medication. Although clinical studies have reported associations between receiving lithium treatment and reduced cancer risk among patients with bipolar disorder, to our knowledge, the association between environmental lithium exposure and cancer risk has never been studied in the general population. Objectives To evaluate the association between exposure to lithium in drinking groundwater and cancer risk in the general population. Design, Setting, and Participants This cohort study included participants with electronic health record and residential address information but without cancer history at baseline from the All of Us Research Program between May 31, 2017, and June 30, 2022. Participants were followed up until February 15, 2023. Statistical analysis was performed from September 2023 through October 2024. Exposure Lithium concentration in groundwater, based on kriging interpolation of publicly available US Geological Survey data on lithium concentration for 4700 wells across the contiguous US between May 12, 1999, and November 6, 2018. Main Outcome and Measures The main outcome was cancer diagnosis or condition, obtained from electronic health records. Stratified Cox proportional hazards regression models were used to estimate the hazard ratios (HRs) and 95% CIs for risk of cancer overall and individual cancer types for increasing quintiles of the estimated lithium exposure in drinking groundwater, adjusting for socioeconomic, behavioral, and neighborhood-level variables. The analysis was further conducted in the western and eastern halves of the US and restricted to long-term residents living at their current address for at least 3 years. Results A total of 252 178 participants were included (median age, 52 years [IQR, 36-64 years]; 60.1% female). The median follow-up time was 3.6 years (IQR, 3.0-4.3 years), and 7573 incident cancer cases were identified. Higher estimated lithium exposure was consistently associated with reduced cancer risk. Compared with the first (lowest) quintile of lithium exposure, the HR for all cancers was 0.49 (95% CI, 0.31-0.78) for the fourth quintile and 0.29 (95% CI, 0.15-0.55) for the fifth quintile. These associations were found for all cancer types investigated in both females and males, among long-term residents, and in both western and eastern states. For example, for the fifth vs first quintile of lithium exposure for all cancers, the HR was 0.17 (95% CI, 0.07-0.42) in females and 0.13 (95% CI, 0.04-0.38) in males; for long-term residents, the HR was 0.32 (95% CI, 0.15-0.66) in females and 0.24 (95% CI, 0.11-0.52) in males; and the HR was 0.01 (95% CI, 0.00-0.09) in western states and 0.34 (95% CI, 0.21-0.57) in eastern states. Conclusions and Relevance In this cohort study of 252 178 participants, estimated lithium exposure in drinking groundwater was associated with reduced cancer risk. Given the sparse evidence and unknown mechanisms of this association, follow-up investigation is warranted.

contiguous United States

Greater sage-grouse habitat of Nevada and northeastern California—Integrating space use, habitat selection, and survival indices to guide areas for habitat management

Executive Summary Greater sage-grouse populations ( Centrocercus urophasianus ; hereafter sage-grouse) are threatened by a suite of disturbances and anthropogenic factors that have contributed to a net loss of sagebrush-dominant shrub cover in recent decades. Declines in sage-grouse populations are largely linked to habitat loss across their range. A key component of conservation and land use planning efforts for sage-grouse involves the continued monitoring and modeling of habitat requirements and suitability across its range. The Bureau of Land Management (BLM) is addressing the management of sage-grouse habitats on BLM-authorized public lands throughout the western United States through a land use planning amendment and associated environmental impact statement (86 FR 66331). More than 25 percent of the range-wide distribution of sage-grouse is within Nevada and northeastern California, and information on sage-grouse distribution and habitat requirements is important to guide appropriate management decisions. Therefore, the BLM has identified the need for updated spatially explicit information on sage-grouse habitat in Nevada and northeastern California to guide the land use planning amendment and associated management decisions. To address this need, researchers with the U.S. Geological Survey, in close cooperation with multiple State and Federal resource agency partners, including BLM, Nevada Department of Wildlife (NDOW) and California Department of Fish and Wildlife (CDFW), sought to map sage-grouse distribution and produce example habitat designations in these states. Herein, we report results of our primary study objective, which was to map sage-grouse habitat and create example habitat management areas, based on more than a decade of location and survival data collected from marked sage-grouse across the study region coupled with lek count survey data managed by the NDOW and the CDFW. We expanded on previously developed methodology to incorporate information on habitat selection and survival during reproductive life stages and specific seasons with updated sage-grouse location and known fate datasets, while also including brood-rearing areas that are understood to be threatened and important for population persistence. We combined predictive habitat map surfaces for each life stage and season with updated information on current occupancy patterns to classify habitat based on its suitability and probability of occupancy. We carried out additional steps to delineate specific example habitat management areas, specifically (1) incorporated corridors connecting key nesting and brood-rearing habitat, (2) corrected outputs for pre-wildfire habitat conditions within areas burned in the last 16 years, and (3) masked out areas of anthropogenic development. Our methodological example of deriving habitat management areas was intended to help inform decisions by BLM and other land managers regarding conservation and management of sage-grouse. Associated data products in the form of habitat maps provide updated, detailed, and comprehensive information about the status of habitats and can be useful to partner agencies in their efforts to designate and rank habitats for this species of high conservation concern in Nevada and California, with full recognition that on-the-ground field data and local sources of information and expertise should be used in conjunction with inferences from these models.

California, Idaho, Nevada

An investigation of shallow ground-water quality near East Fork Poplar Creek, Oak Ridge, Tennessee

Alluvial soils and fill materials in and near the floodplain of East Fork Poplar Creek in Oak Ridge, Tennessee, are contaminated with various trace metals (primarily mercury), organic compounds, and radionuclides that were lost to the stream as a result of past operations at the Y-12 Plant, a nuclear-processing facility located within the U.S. Department of Energy’s Oak Ridge Reservation. Observation wells were installed in the shallow (above bedrock) aquifer in and near the floodplain of East Fork Poplar Creek and water-quality samples were collected to determine if contaminants found in the soils and fill are also present in the shallow ground water. The shallow aquifer in the East Fork Poplar Creek flood plain consists primarily of alluvial silt and clay with lesser amounts of sand and gravel. Thickness of the shallow aquifer ranges from essentially zero to as much as 20 feet. A silty-clay glei horizon is present between the base of the alluvium and the top of bedrock at most flood-plain locations and, where present, likely impedes downward ground-water movement. Water in the shallow aquifer near East Fork Poplar Creek occurs under water-table conditions. Recharge to the shallow aquifer is principally from precipitation, and discharge is through springs and seeps to East Fork Poplar Creek and its tributaries. During spring, summer; and fall, evapotranspiration also accounts for the removal of water in storage in the shallow aquifer. Water levels in the shallow aquifer fluctuate seasonally in response to variations in recharge and evapotranspiration. Generally, the depth to water in the observation wells ranged from about 1 to 4 feet below land surface in late winter, and from about 2 to 7 feet below land surface in late fall. During extremely dry periods, the water table recedes below the top of bedrock in some flood-plain areas, possibly causing East Fork Poplar Creek to lose water to the shallow aquifer along some reaches. Contaminants found in water samples collected from several of the observation wells in concentrations (total and (or) total-recoverable) which equaled or exceeded drinking-water standards established by the U.S. Environmental Protection Agency are antimony, chromium, lead, mercury, selenium, total phenols, and strontium-90. Total and dissolved uranium concentrations exceeded 1.0 microgram per liter in samples from nearly 70 percent of the wells in the East Fork Poplar Creek flood plain. Organic compounds that were identified in low concentrations in samples from a few wells in the flood plain are: Arochlor 1260, benzo(a)anthracene, benzo(b)fluoranthene, chrysene, 3,3-dichlorobenzidine, di-n-butylphthalate, N-nitrosodiphenylamine, and pyrene. Water from one well in the East Fork Poplar Creek flood plain at a contaminated fill site contained 37and 8 micrograms per liter of trichloroethene and trans-1,2-dichloroethene, respectively. Comparison of the results of total and (or) total-recoverable trace-metal determinations with those from dissolved determinations demonstrates that elevated concentrations of these substances in water collected from several of the wells in the East Fork Poplar Creek flood plain resulted from sorption of trace metals (and possibly organic compounds and radionuclides) by fine sediment suspended in the samples. The occurrence of contaminated sediment in these samples is suspected to be the result of borehole contamination during well installation.

Tennessee

Coastal barrier resilience and resistance: Analysis and metrics for characterizing coastal state

Barrier islands are shaped by a variety of short- and long-term environmental processes such as storms and relative sea-level rise. These islands, found along the estuarine-marine interface, provide ecosystem services including storm surge and wave attenuation, erosion protection to inland marshes, habitat for fish and wildlife, and recreation. Natural resource managers require actionable information on how barrier island resilience and resistance changes over time to understand how an island’s current state relates to past conditions and to inform restoration prioritization and implementation. The U.S. Geological Survey and The Water Institute collaborated on a study to develop indicators of resilience and resistance for barrier islands in Louisiana. Here, resilience captures island persistence on yearly to decadal time scales, and resistance captures persistence on event time scales of days to weeks. The indicators fall in two categories: Tier 1 Screening Metrics, that can be readily calculated from available data, are easily interpretable as an evaluation of barrier condition, and provide a high-level snapshot of overall barrier resilience and resistance; and Tier 2 Analysis Metrics, which are detailed metrics that required specialized analysis and interpretation and are more applicable to answering specific questions managers may have about barrier state. The research team derived Tier 1 resilience indicators from subaerial land and vegetation cover calculated from publicly available maps and products based on satellite imagery. By benchmarking the total land and vegetation extent against their respective historical maxima, this metric provides a snapshot of an island’s current state in the context of its long-term trajectory. The research team developed Tier 1 resistance indicators based on subaerial island configuration and water level recurrence as a proxy for evaluating island resistance to storms, which are the primary driver of short-term change. These Tier 1 metrics can be analyzed over time to provide a high-level assessment of how an island’s resistance decreases because of elevation loss or sea-level rise or increases due to restoration or natural recovery. The research team developed Tier 2 resilience and resistance indicators and associated analyses to provide detailed information for specific time periods or applications (e.g., wildlife management). These metrics include habitat coverage from high-resolution maps, which show composition changes over time to capture the evolving resilience of specific habitat types; high tide flooding analysis, which evaluate island area relative to specified flooding thresholds to characterize resistance in the short-term or, if analyzed over time, indicate changes in resilience; and hypsometric curve analysis, which allows managers to evaluate island area changes above their own elevation benchmarks of interest and similarly characterize resistance in the short-term or indicate changes in resilience if assessed over time. The research team calculated Tier 1 metrics of the barrier islands and headlands along the coast of Louisiana for the period of 1984 through 2021 and Tier 2 metrics for select times during that period depending on data available and quality. The results were captured in a report card for each barrier, which also includes an overview of the metrics and their interpretation; a restoration and storm history; and Tier 1 and Tier 2 metric analysis, including benchmarking against coastwide and regional values as well as to an island’s pre-restoration trajectory. These report cards provide a readily digestible synthesis of barrier condition and trajectory that coastal managers can use to support restoration prioritization and other decisions.

Louisiana

Hydrogeologic framework and extent of saltwater intrusion in Kings, Queens, and Nassau Counties, Long Island, New York

In 2016, the U.S. Geological Survey began a multiyear cooperative study with the New York State Department of Environmental Conservation to evaluate the sustainability of Long Island’s sole-source aquifer system through hydrogeologic mapping, compilation of groundwater chloride concentrations, and groundwater flow modeling. In the initial phase of the islandwide study, the hydrogeologic framework and extent of saltwater intrusion in aquifers in Kings, Queens, and Nassau Counties on western Long Island, N.Y., were investigated. The aquifer system underlying western Long Island has been under stress from pumping of public, irrigation (golf course), and industrial supply wells. Saltwater intrusion has occurred from surrounding embayments (East River, Long Island Sound, Jamaica Bay, and the Atlantic Ocean) due to pumping. Eighteen boreholes were drilled and cores taken during 2019–21 to collect hydrogeologic, geochemical, and geophysical data to delineate the complex subsurface hydrogeology and extent of saltwater intrusion within the study area. Evaluation of the new cores, reexamination of legacy core descriptions, and analysis of borehole geophysical logs was used to refine the previously published hydrogeologic framework of Pleistocene and Cretaceous unconsolidated sediments in the area, including delineation of a previously undefined hydrogeologic unit between the Magothy aquifer and the Raritan confining unit, herein named the “upper Raritan aquifer.” The upper Raritan aquifer was first recognized in southeastern Nassau County from an analysis of about 50 closely spaced boreholes with high-resolution core descriptions and gamma-ray (gamma) logs. Further analysis of borehole logs across the study area indicated that the upper Raritan aquifer was also present in Kings and Queens Counties. Nuclear magnetic resonance (NMR) logging was used for the first time on Long Island to provide estimates of the hydraulic properties of the major aquifer and confining units. Unlike other geophysical logs that record responses to the rock matrix and fluid properties and are strongly dependent on mineralogy, NMR logs record responses to the presence of hydrogen protons in the formation fluid to determine water fraction and pore-size distribution. NMR log analysis provided estimates of the clay-bound, capillary-bound, and mobile water fractions and hydraulic conductivity of aquifers and confining units penetrated by five wells in Nassau County. Pumpage for public-supply and industrial wells on Long Island began in the 1870s with small, localized suppliers of populated areas in Kings and Queens Counties. By 1904–16, pumpage for public water supply in Kings County averaged 21 million gallons per day, and averaged 37 million gallons per day in Queens County, mostly from the upper glacial aquifer. Saltwater intrusion was reported as early as the beginning of the 20th century and included the upper glacial-Jameco-Magothy and Lloyd-North Shore aquifer systems. By 1936, pumping in central Kings County created a major cone of depression in the water table extending to the south shore of much of Kings County and into southwestern Queens County. Saltwater intrusion has caused the shutdown of public-supply wells in Kings, Queens, and Nassau Counties. A large saltwater intrusion wedge in the Lloyd aquifer was indicated in southern Queens County in the early part of the 20th century, and the saltwater interface may have been onshore predevelopment. Most of Kings and Queens Counties are intruded with saltwater in both the upper glacial-Jameco-Magothy and Lloyd-North Shore aquifers systems. Saltwater increased during the 20th century and continues to increase to the present (2023) in the Lloyd-North Shore aquifer system in Great Neck and Manhasset Neck in northern Nassau County. A major wedge of saltwater intrusion in the upper glacial-Jameco-Magothy aquifer in southwestern Nassau County appears to be increasing.

New York

Correlation analysis of groundwater and hydrologic data, Kaloko-Honokōhau National Historical Park, Hawai‘i

Designated in 1978, Kaloko-Honokōhau National Historical Park is located on the west coast of the Island of Hawaiʻi. The Kaloko-Honokōhau National Historical Park encompasses about 1,200 acres of coastal land and nearshore ecosystems, which include wetlands, anchialine pools (landlocked bodies of brackish water with hydrologic connections to the ocean), fishponds, a fishtrap, and coral reefs. These nearshore ecosystems are dependent on groundwater discharge with a freshwater component and provide habitat for threatened and endangered, endemic species, such as the orangeblack Hawaiian damselfly ( Megalagrion xanthomelas ) and the Hawaiian coot (ʻAlae keʻokeʻo, Fulica alai ). The populations of these native species, however, are threatened because of habitat loss related to urban development and environmental changes. Kaloko-Honokōhau National Historical Park is within the Keauhou aquifer system and the North Kona District, which experienced a 52 percent resident-population increase between 2000 and 2020 and a 41 percent visitor increase between 2008 and 2019. To support the current water demand associated with this growing population, groundwater is the primary source of freshwater used in the North Kona District, with about 15 million gallons of groundwater withdrawn from the Keauhou aquifer system per day since 2009. With anticipated development, future (2015–35) groundwater withdrawal from the Keauhou aquifer system is projected to be about 55 percent greater than recent (2012–14) withdrawal. Because Kaloko-Honokōhau National Historical Park is located within a coastal aquifer, natural and human-induced changes can affect the quality and quantity of groundwater, which can threaten groundwater-dependent ecosystems. To improve understanding of recent groundwater conditions, the U.S. Geological Survey, in cooperation with the National Park Service, undertook this study to document correlations between hydrologic time-series datasets from sites in and near Kaloko-Honokōhau National Historical Park using the nonparametric (distribution-free) Kendall’s tau statistical test. For the statistical analyses, dependent variables representing the groundwater system include groundwater level, the groundwater-level difference between pairs of sites, and specific conductance, and independent variables include datasets of sea level, rainfall, and groundwater withdrawal. About 34 percent of the 140 non-time-lagged Kendall’s tau statistical tests evaluated in this report are statistically significant ( p -value ≤ 0.050) with generally weak (0.1 ≤ tau ≤ 0.2) to moderate (0.2 ≤ tau ≤ 0.3) correlations. Groundwater levels measured at monitoring sites have the strongest correlation with the multivariate El Niño–Southern Oscillation index and withdrawal from production wells at the nearby Kohanaiki Private Club Community. Specific conductance is not consistently and significantly correlated with the independent hydrologic variables investigated in this report. Because the relations between hydrologic variables are commonly not instantaneous, a second set of correlations was evaluated after applying a range of time lags to the independent variable datasets. Relative to the non-time-lagged case (the set of correlations that did not use time-lagged independent variables), some of the time-lagged independent variables improved correlations with some of the dependent variables. For a particular independent variable, similar time lags were expected between the independent variable and dependent variable at all four monitoring sites. However, different time lags among the four sites sometimes produced the strongest correlations. This study identified several correlations that are statistically significant and hydrologically plausible, but the correlations could indicate that multiple concurrent factors are controlling the observed groundwater-system response, which might be better addressed using multivariate analyses. This study only investigates bivariate correlations, which may not explain all the variance in the data. The correlations analyzed in this report are limited by the quantity of available hydrologic data in the area near Kaloko-Honokōhau National Historical Park and are based on 14 years of time-series data, which were aggregated to a relatively coarse monthly temporal resolution that represents the minimum resolution common to all datasets.

Hawaii

Quality of groundwater used for domestic supply in the Gilroy-Hollister basin and surrounding areas, California, 2022

More than 2 million Californians rely on groundwater from domestic wells for drinking-water supply. This report summarizes a 2022 California Groundwater Ambient Monitoring and Assessment Priority Basin Project (GAMA-PBP) water-quality survey of 33 domestic and small-system drinking-water supply wells in the Gilroy-Hollister Valley groundwater basin and the surrounding areas, where more than 20,000 residents are estimated to utilize privately owned domestic wells. The study area includes the Llagas subbasin in the north, the North San Benito subbasin in the south, and the surrounding uplands. The study was focused on groundwater resources used for domestic drinking-water supply, which are mostly drawn from shallower parts of aquifer systems rather than those of groundwater resources used for public drinking-water supply in the same area. This assessment characterized the quality of ambient groundwater in the aquifer before filtration or treatment, rather than the quality of drinking water delivered to the tap. To provide context, the measured concentrations of constituents in groundwater were compared to Federal and California State regulatory and non-regulatory benchmarks for drinking-water quality. A grid-based method was used to estimate the areal proportions of groundwater resources used for domestic drinking wells that have water-quality constituents present at high concentrations (above the benchmark), moderate concentrations (between one-half of the benchmark and the benchmark for inorganic constituents, or between one-tenth of the benchmark and the benchmark for organic constituents), and low concentrations (less than one-half or one-tenth the benchmark for inorganic and organic constituents, respectively). This method provides statistically representative results at the study-area scale and permits comparisons to other GAMA-PBP study areas. In the study area, inorganic constituents in groundwater were greater than regulatory benchmarks (U.S. Environmental Protection Agency [EPA] or State of California maximum contaminant levels [MCLs]) for public drinking-water quality in 24 percent of domestic groundwater resources. The inorganic constituents present at concentrations greater than MCLs for drinking water were nitrate (as nitrogen), barium, chromium, and selenium. Total dissolved solids (TDS) or manganese were present at concentrations greater than the secondary maximum contaminant levels (SMCLs) that the State of California uses as aesthetic-based benchmarks in 48 percent of domestic groundwater resources. No volatile organic compounds or pesticide constituents were present at concentrations greater than regulatory benchmarks. Total coliform bacteria and enterococci were detected in 4 percent of domestic groundwater resources. Per- and polyfluoroalkyl substances (PFAS) were detected in 19 percent of domestic groundwater resources, and 10 percent had concentrations greater than recently enacted (April 2024) EPA MCLs. Physical and chemical factors from natural and anthropogenic sources that could affect the groundwater quality were evaluated using results from statistical testing of associations between constituent concentrations and potential explanatory variables. In this study, relevant physical factors include well construction characteristics, groundwater age, site proximity to groundwater recharge or discharge zones, and potential sources of contamination. Relevant chemical factors include the initial chemistry of the recharge water, the mineralogy of the aquifer sediments, and the subsequent shifts in chemistry as biologic and geologic reactions alter groundwater in the subsurface. Nitrate concentrations were correlated to agricultural land use, distance from the boundary of the Gilroy-Hollister Valley groundwater basin, and the proportion of modern (post-1950s) water captured by the well. Denitrification under anoxic redox conditions can mitigate some nitrate derived from fertilizer application. Total dissolved solids primarily were derived from water-rock interactions with soils and aquifer materials in the study area, but there were high concentrations where agricultural practices contributed additional TDS. Mineralogy of aquifer sediments and rocks also affect barium, selenium, boron, and chromium concentrations in the Gilroy-Hollister Valley groundwater basin. PFAS were positively correlated with urban land use and the proportion of modern water captured by the well.

California

Potentiometric surface maps and groundwater-level hydrographs for confined aquifers of the New Jersey Coastal Plain, 2018

The U.S. Geological Survey, in cooperation with the New Jersey Department of Environmental Protection (NJDEP), prepared potentiometric surface maps for 10 confined aquifers of the New Jersey Coastal Plain physiographic province based on water-level measurements collected during late 2018 and early 2019 from 951 wells in New Jersey and parts of Pennsylvania and Delaware. Maps were prepared for the confined Cohansey aquifer, Rio Grande water-bearing zone, Atlantic City 800-foot sand, Piney Point aquifer, Vincentown aquifer, Wenonah-Mount Laurel aquifer, Englishtown aquifer system, and the upper, middle, and lower aquifers of the Potomac-Raritan-Magothy aquifer system. Potentiometric surface maps indicate regional cones of depression in the following aquifers and the counties in which they are centered: Atlantic City 800-foot sand in Atlantic County, the Piney Point aquifer in Cumberland County, the Wenonah-Mount Laurel aquifer and Englishtown aquifer system in Monmouth and Ocean Counties, the Wenonah-Mount Laurel aquifer in Camden and Gloucester County, the upper aquifer of the Potomac-Raritan-Magothy aquifer system in Ocean County, and the upper, middle, and lower aquifers of the Potomac-Raritan-Magothy aquifer system in Camden County. Cones of depression with smaller areal extents were in the confined Cohansey aquifer, the Rio Grande water-bearing zone, the Atlantic City 800-foot sand centered in Cape May County, the Piney Point aquifer centered in Ocean County, the Wenonah-Mount Laurel aquifer in Salem and Burlington Counties, the Englishtown aquifer system in Camden County, the upper aquifer of the Potomac-Raritan-Magothy aquifer system in Monmouth County, and the middle aquifer of the Potomac-Raritan-Magothy aquifer system in Monmouth, Ocean, and Salem Counties. No cone of depression was interpreted in the Vincentown aquifer. Long-term hydrographs are presented for 75 wells spanning each of the 10 confined aquifers, and contain a mix of discrete water-level measurements and daily mean water levels based on continuously recorded 15-minute data. Changes of water levels during 2014–19, as indicated by the hydrographs, were compared with those of previous periods to assess any departures from historical data. During 2014–19, water levels were stable and fluctuated within similar ranges as previous periods in the following aquifers and locations: all wells in the confined Cohansey aquifer, the Rio Grande water-bearing zone, the Vincentown aquifer, the Englishtown aquifer system, the Piney Point aquifer wells in Burlington and Ocean Counties, six of eight wells in the Wenonah-Mount Laurel aquifer, all wells in the upper and lower aquifers of the Potomac-Raritan-Magothy aquifer system outside NJDEP Critical Areas, and all wells in the middle aquifer of the Potomac-Raritan-Magothy aquifer system except those within NJDEP Critical Area II. Increasing water levels in 2014–19, ongoing since historical periods, were indicated in the following aquifers and locations: Atlantic County wells in the Piney Point aquifer, all wells in the upper and lower aquifers of the Potomac-Raritan-Magothy aquifer system outside NJDEP Critical Areas, and all wells in the middle aquifer of the Potomac-Raritan-Magothy aquifer system within NJDEP Critical Area II. Water levels in the Atlantic City 800-foot sand also increased during 2014–19 in wells in Atlantic County and northern Cape May County closer to the center of the cone of depression in that aquifer, which is a response unique to this period and absent from previous periods. During 2013–19, continued decreasing water levels, ongoing since previous periods, were indicated by hydrographs of Atlantic City 800-foot sand wells in southern Cape May County, Piney Point aquifer wells in Cumberland County where the regional cone of depression is located, and two wells in the Wenonah-Mount Laurel aquifer—070478, which in 2014–19 departed from previous periods, and 330020, which continued a gradual decrease throughout its period of record.

New Jersey

Factors affecting the distribution of water-bearing fractures in the bedrock aquifers of West Virginia

Bedrock aquifers cover 23,601 square miles within the State of West Virginia and comprise 97.4 percent of the surficial area within the State; the remaining 2.6 percent (621 square miles) consists of alluvial sand-and-gravel and glacial outwash aquifers bordering the State’s major rivers. While West Virginia’s alluvial aquifers have been studied extensively, bedrock aquifers have only been characterized for studies completed in a few areas in Jefferson, McDowell, and Monroe Counties. Bedrock aquifers are water supplies for public supply, agriculture, industry, and residential homeowner use. In this study, the U.S. Geological Survey, in cooperation with the West Virginia Department of Environmental Protection Division of Water and Waste Management, provides a statewide assessment of the occurrence and distribution of fractures within bedrock aquifers of the State and the various topographic, physiographic, and lithologic influences controlling the occurrence and distribution of bedrock fractures. The results of this study provide an increased understanding of the distribution of fractures in bedrock aquifers in West Virginia and help to verify trends that have been suspected for many years but were never well documented or verified by data. The results confirmed that the density of fractures and those that were determined to be water bearing decrease significantly with depth. A statistically significant difference in the density of fractures was observed at a depth of 215 feet for wells in the Appalachian Plateaus Physiographic Province’s and in the Valley and Ridge Physiographic Province’s aquifers; a higher density of fractures and water-bearing fractures were above a depth of 215 feet than below that depth. This is an important consideration when drilling wells for residential, commercial, industrial, or agricultural water supply. Abandoned underground coal mines are commonly believed to form large pools of water in the interconnected mine entries in abandoned room and pillar coal mines. Such pools of water can and do exist in abandoned underground coal mines, but many mines lack open entries and are held up by overburden strata and pillars that can collapse and form aquifers comprised of vast interconnected rubble zones (gob), especially in older mines. Data assessed for this study showed that shale-corrected values of effective porosity for limestone aquifers in West Virginia had a median value of 2 percent and an average value of 4 percent and generally are mineralized with low effective porosity. Argillaceous or sandy limestone has a median shale-corrected porosity of 4 percent and an average shale-corrected porosity of 5 percent. The median and average shale-corrected porosity of sandstone aquifers was estimated to be 14 percent, but the median shale-corrected porosity for argillaceous or calcareous sandstone was 5 percent and the average shale-corrected porosity for argillaceous or calcareous sandstone was 6 percent. Even though shale has a relatively high total sonic porosity compared to other lithologies, shale and siltstone had relatively low shale-corrected porosity, ranging from 0 to 2 percent. Well yields were previously documented to be highest in valley settings, lowest on hilltops, and intermediate on hillsides. Transmissivity data provided by this study confirm this general pattern within the Appalachian Plateaus Province; however, the Valley and Ridge Province does not follow this pattern. While still lowest on hilltop settings, the highest well yields were in hillside settings. The trend for the Valley and Ridge Province was likely skewed because of 9 high-yield wells specifically targeting deeper thin limestone units, such as the Tonoloway and Helderberg Limestones, at depths with transmissivity in excess of 2,000 feet squared per day in Mineral County, West Virginia, or targeting karst aquifers in Berkeley, Jefferson, or Greenbrier Counties, West Virginia. Finally, water-bearing fractures have been hypothesized to comprise a small number of all fractures within a typical bedrock well in West Virginia. Data collected for this study support this theory. A total of 3,403 fractures were identified during this study; 3,151 (92.6 percent) of those fractures are low-transmissive, and only 252 (7.4 percent) fractures are water-bearing. Even though a well may contain many fractures, less than 8 percent are considered water-bearing fractures.

West Virginia