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At least 199 records · Page 11Linked to original sources

Toxicity to woodlice of zinc and lead oxides added to soil litter

Previous studies have shown that high concentrations of metals in soil are associated with reductions in decomposer populations. We have here determined the relation between the concentrations of lead and zinc added as oxides to soil litter and the survival and reproduction of a decomposer population under controlled conditions. Laboratory populations of woodlice (Porcellio scaber Latr) were fed soil litter treated with lead or zinc at concentrations that ranged from 100 to 12,800 ppm. The survival of the adults, the maximum number of young alive, and the average number of young alive, were recorded over 64 weeks. Lead at 12,800 ppm and zinc at 1,600 ppm or more had statistically significant (p < 0.05) negative effects on the populations. These results agree with field observations suggesting that lead and zinc have reduced populations of decomposers in contaminated forest soil litter, and concentrations are similar to those reported to be associated with reductions in natural populations of decomposers. Poisoning of decomposers may disrupt nutrient cycling, reduce the numbers of invertebrates available to other wildlife for food, and contribute to the contamination of food chains.

Ambio

Vegetation changes over 12 years in ungrazed and grazed Conservation Reserve Program Grasslands in the central and southern plains

The Conservation Reserve Program (CRP) established under the 1985 Food Security Act has the fundamental objectives of jointly providing economic support to segments of the agricultural community and conservation of natural resources (Osborn, 1997; Heard and others, 2000). Although soil loss on highly erodable lands was the principal natural resource conservation issue addressed in the 1985 CRP, improving water quality and wildlife habitat both became important considerations as the program evolved (Farmer and others, 1988). For example, Best and others (1997) found that production of young birds on CRP fields in the Midwest was ≥15 times the production on row-crop fields because of improved habitat. The increasing importance of wildlife habitat is reflected in continuing refinement of the Environmental Benefits Index (EBI) used by the U.S. Department of Agriculture (USDA) to quantify the potential benefits of enrolling lands in CRP (Osborn, 1997; Ribaudo and others, 2001). The refinements reflect input furnished by federal, state, and non-government organizations seeking greater wildlife habitat quality on CRP lands (Roseberry and David, 1994; Hughes and others, 1995; Millenbah and others, 1996; Patterson and Best, 1996; Rodgers, 1999; Allen and others, 2001). Refinement in the EBI has changed the types of grasses planted on newly enrolled land. In early CRP signups (1 through 11), 71% of new grassland acres were planted to introduced grasses and legumes [Conservation Practice (CP) 1] while 29% of the acres were planted to native grasses (CP2) (Osborn and others, 1992). By the 27th signup in July 2004, over 34.8 million acres (14 million ha) were enrolled in the CRP. More than 73% of these lands were planted to various mixtures of introduced (CP1) or native (CP2) grasses for a minimal contract period of 10 years (USDA, 2004). Continuation of grass plantings under the 2002 Farm Bill may result in CRP lands furnishing grass dominated cover for 20 or more consecutive years. The species of grass established in seeded grasslands can have a major influence on the potential quality of wildlife habitat where vegetation is maintained over a multi-year period. Different species of grass may have comparable abilities to alleviate soil erosion but furnish dissimilar qualities of wildlife habitat (fig. 1). For example, smooth brome, an introduced cool-season grass (grass species and scientific names are presented in table 1), is highly valued for its erosion control and forage attributes (Casler and Carlson, 1995). Switchgrass, a native warm-season grass, also is valued for its soil and water conservation qualities (Moser and Vogel, 1995) but provides greater benefits for some species of wildlife (Clubine, 1995). The quality of nesting and winter cover for ring-necked pheasants (Phasianus colchicus) furnished by smooth brome on northeastern Colorado CRP lands is inferior to that provided by the taller, more robust switchgrass (Allen, 1994). Characteristics of the agricultural landscape surrounding individual CRP fields also play a role in the wildlife habitat potential of CRP plantings (Weber and others, 2002; Nusser and others, 2004). of native and seeded grasslands change in response to the presence (and absence) of physical disturbances such as fire, grazing, tillage, and haying (Hobbs and Huenneke, 1992; Millenbah and others, 1996; Allen and others, 2001; Renfrew and Ribic, 2001; Swengel and Swengel, 2001). The perpetuation of diversity in species composition and vegetation structure following disturbance sustains desirable habitat for a variety of grassland-dependent wildlife (Hall and Willig, 1994; Barnes and others, 1995; King and Savidge, 1995; Granfors and others, 1996; Herkert and others, 1996; Kurzejeski, 1996; Patterson and Best, 1996; Klute and others, 1997). Undisturbed grasslands have lower grass and forb species diversity, greater amounts of dead plant material, decreased as well as seasonally delayed productivity, and diminished structural diversity of vegetation (Peet and others, 1975; Rice and Parenti, 1978; Butler and Briske, 1988; Campa and Winterstein, 1992). Recommendations for the timing of disturbance to increase grass and forb species diversity range from 3 to 8 years following establishment of seeded grasslands in the northern Great Plains and Midwest (Duebbert and others, 1981; Higgens, 1987; Millenbah and others, 1996). The management interval, however, is affected by climatic conditions, soils, grass species, and management history of the individual stand. We quantified changes in vegetation structure and species composition across the typical 10-year contract period in undisturbed southern and central Great Plains CRP fields (fig. 2) planted to introduced and native grasses. In addition, we compared changes in vegetation in fields grazed during the emergency release of 1996 by comparing conditions prior to grazing and two and four years post grazing relative to changes in similar fields that were not grazed. Documentation of long-term changes in vegetation structure and composition for fields planted to common grass seed mixtures across a wide range of environmental conditions provides information to improve long-term wildlife habitat potential, guide program administration, and define management practices that yield economic benefits to operators while still meeting wildlife and conservation objectives. Emergency grazing provisions of the CRP are controversial. Although grazing can alter vegetative characteristics and reduce habitat quality in the short-term (Temple and others, 1999), periodic disturbance may be necessary to maintain habitat quality, and more information is needed assessing long-term effects of emergency grazing on vegetative structure and species composition.

Conference Paper

Thermal variability drives synchronicity of an aquatic insect resource pulse

Spatial heterogeneity in environmental conditions can prolong food availability by desynchronizing the timing of ephemeral, high‐magnitude resource pulses. Spatial patterns of water temperature are highly variable among rivers as determined by both natural and anthropogenic features, but the influence of this variability on freshwater resource pulse phenology is poorly documented. We quantified water temperature and emergence phenology of an aquatic insect (salmonfly, Pteronarcys californica ) resource pulse in two rivers characterized by differing catchment topography and human impact. Along both rivers, salmonfly emergence occurred earlier where spring temperatures were warmer. Emergence events were brief (4–8 d) at sites in the more human‐impacted river, but occurred asynchronously along the entire river, lasting 27 d in total. In contrast, emergence events were more prolonged (6–11 d) at sites on the more natural and topographically complex river, but occurred synchronously along the entire river, lasting 13 d in total. These scale‐specific differences in subsidy duration could have opposing consequences for salmonfly consumers depending on their mobility and foraging habits. Asynchronous emergence at a large scale is potentially most important for mobile consumers like birds and fish that can migrate to feed on aquatic insects and track resource waves across a landscape, whereas prolonged emergence duration at a smaller scale may be most important for immobile or opportunistic consumers like spiders and ants. Relating environmental heterogeneity and resource pulse phenology across a gradient of human impact and at multiple spatial scales is needed for a better understanding of how food availability, aquatic–terrestrial linkages, and consumer–resource dynamics may change with climate variability and increasing human activity in the future.

Montana

Salmon, forage fish, and kelp

Kelp beds are prominent features of northeast Pacific coastlines. They are seasonal in nature, as are the communities that use them. Here, juvenile and adult Chinook salmon ( Oncorhynchus tshawytscha ) – key components of northeast Pacific marine food webs that link plankton and forage fishes to endangered killer whales – have just arrived at the coastal kelp beds (left) and are feeding on the large schools of forage fish such as young‐of‐the‐year herring, which are also migrating in great numbers near the shore. Juvenile herring and smelt will soon move offshore to grow and feed, and finally return as adults to spawn along shorelines. Rapid growth is critical to the survival of young salmon. They quickly learn to work together to herd the small, skittish prey into tight groups. The kelp beds play an important role for both the salmon and their prey, providing refuge for feeding salmon and enhanced prey resources for hungry forage fish, which in turn feed incessantly at the surface of the kelp beds, except when they are disrupted by lightning‐fast attacks by marauding salmon. By October, much of the kelp will be gone, as will the juvenile salmon and forage fish, replaced by their adult congeners (right) that have traveled for years and hundreds of miles to continue the cycle. Globally, kelp forests are in flux. Disturbances, including those induced by climate change, may have serious implications not only for this critical nearshore phase of salmon and forage fish, but also for the future viability of our cold‐water northeast Pacific marine ecosystems.

Frontiers in Ecology and the Environment

Managing a subsidized predator population: Reducing common raven predation on desert tortoises

Human communities often are an inadvertent source of food, water, and other resources to native species of wildlife. Because these resources are more stable and predictable than those in a natural environment, animals that subsist on them are able to increase in numbers and expand their range, much to the detriment of their competitors and species they prey upon. In the Mojave Desert, common ravens (Corvus corax) have benefited from human-provided resources to increase in population size precipitously in recent years. This trend has caused concern because ravens prey on juvenile desert tortoises (Gopherus agassizii), a federally threatened species. In this paper, I discuss management strategies to reduce raven predation on desert tortoises. The recommendations fall into three categories: (1) managing raven populations by reducing access to anthropogenic resources; (2) removing offending ravens or other birds in specially targeted tortoise management zones; and (3) continuing research on raven ecology, raven behavior, and methods of reducing raven predation on tortoises. I also recommend approaching the problem within an adaptive management framework: management efforts should first be employed as scientific experiments - with replicates and controls - to yield an unbiased assessment of their effectiveness. Furthermore, these strategies should be implemented in concert with actions that reduce other causes of desert tortoise mortality to aid the long-term recovery of their populations. Overall, the approaches outlined in this paper are widely applicable to the management of subsidized predators, particularly where they present a threat to a declining species of prey.

Environmental Management

Native amphibian toxin reduces invasive crayfish feeding with potential benefits to stream biodiversity

Background Biodiversity is generally reduced when non-native species invade an ecosystem. Invasive crayfish, Procambarus clarkii , populate California freshwater streams, and in the Santa Monica Mountains (Los Angeles, USA), their introduction has led to trophic cascades due to omnivorous feeding behavior and a rapid rate of population growth. The native California newt, Taricha torosa , possesses a neurotoxin, tetrodotoxin (TTX), that affects freshwater animal behavior. Given P. clarkii has a limited evolutionary history with TTX, we hypothesized that TTX may affect crayfish feeding behaviors. To determine if TTX affects P. clarkii behavior, we measured cumulative movement and various feeding behaviors of P. clarkii exposed to (i) waterborne, ecologically realistic concentrations of TTX (~ 3.0 × 10 − 8 moles/L), (ii) an anuran chemical cue to account for intraguild cues, or (iii) a T. torosa chemical cue with quantitated TTX in it (~ 6.2 × 10 − 8 moles/L). Results We found that the presence of TTX in any form significantly reduced crayfish movement and decreased the amount of food consumed over time. Crayfish responses to the anuran treatment did not significantly differ from controls. Conclusion Our laboratory results show that naturally occurring neurotoxin from native California newts limits invasive crayfish foraging and feeding rates, which may play a role in preserving local stream ecosystems by limiting invasive crayfish behaviors that are detrimental to biodiversity.

California

Associations between iron concentration and productivity in montane streams of the Black Hills, South Dakota

Iron is an important micronutrient found in aquatic systems that can influence nutrient availability (e.g., phosphorus) and primary productivity. In streams, high iron concentrations often are associated with low pH as a result of acid mine drainage, which is known to affect fish and invertebrate communities. Streams in the Black Hills of South Dakota are generally circumneutral in pH, yet select streams exhibit high iron concentrations associated with natural iron deposits. In this study, we examined relationships among iron concentration, priphyton biomass, macroinvertebrate abundance, and fish assemblages in four Black Hills streams. The stream with the highest iron concentration (~5 mg Fe/L) had reduced periphyton biomass, invertebrate abundance, and fish biomass compared to the three streams with lower iron levels (0.1 to 0.6 mg Fe/L). Reduced stream productivity was attributed to indirect effects of ferric iron Fe +++ ), owing to iron-hydroxide precipitation that influenced habitat quality (i.e., substrate and turbidity) and food availability (periphyton and invertebrates) for higher trophic levels (e.g., fish). Additionally, reduced primary and secondary production was associated with reduced standing stocks of salmonid fishes. Our findings suggested that naturally occurring iron deposits may constrain macroinvertebrate and fish production.

South Dakota

Tests in a semi-natural environment suggest that bait and switch strategy could be used to control invasive Common Carp

Common Carp ( Cyprinus carpio Linnaeus, 1758), is a highly invasive species that has had profound effects on biodiversity and ecosystem services. Many Carp management methods have been applied including physical removal, pesticide treatments of whole lakes, and water drawdowns. Herein, we tested key elements of a potential “bait and switch” approach in which corn could be used to induce feeding aggregations of Carp and then switched for corn pellets with a pesticide Antimycin-A (ANT-A) to selectively target the Carp. First, laboratory experiments were used to determine if addition of lethal concentrations of ANT-A to corn pellets deterred Carp from eating corn-based food pellets. Second, a pond experiment tested if a corn-based bait containing ANT-A functioned as a species-specific Carp management tool in a semi-natural environment with three common native fishes: White Sucker ( Catostomus commersonii Lacepède, 1803), Yellow Perch ( Perca flavescens Mitchill, 1814), and Bluegill ( Lepomis macrochirus Rafinesque, 1819). The use of baited sites by Carp and native species was monitored using passive integrated transponder (PIT) tags. Mortality of each species and presence of corn in their digestive tracts was also monitored. Our laboratory experiment showed that presence of ANT-A did not deter Carp from consuming the pellets. The pond experiment showed that only Carp perished in significant numbers once toxic bait was applied. Further, only Carp increased their use of baited sites once baiting began, and only Carp had corn in their digestive tracts. Overall, our results indicate that corn might function as a species-specific Carp attractant in systems of North American Midwest and that corn-based bait can be used to effectively conceal a lethal dose of ANT-A. Further research is necessary to refine this potential management tool, specifically investigating the behavioral and social dynamics of Carp aggregating at sites baited with corn to enhance the temporal and spatial specificity of pesticide application.z

Management of Biological Invasions

Orbital forcing of deep-sea benthic species diversity

Explanations for the temporal and spatial patterns of species biodiversity focus on stability-time, disturbance-mosaic (biogenic microhabitat heterogeneity) and competition-predation (biotic interactions) hypotheses. The stability-time hypothesis holds that high species diversity in the deep sea and in the tropics reflects long-term climatic stability. But the influence of climate change on deep-sea diversity has not been studied and recent evidence suggests that deep-sea environments undergo changes in climatically driven temperature and flux of nutrients and organic-carbon during glacial-interglacial cycles. Here we show that Pliocene (2.85-2.40 Myr) deep-sea North Atlantic benthic ostracod (Crustacea) species diversity is related to solar insolation changes caused by 41,000-yr cycles of Earth's obliquity (tilt). Temporal changes in diversity, as measured by the Shannon- Weiner index, H(S), correlate with independent climate indicators of benthic foraminiferal oxygen-isotope ratios (mainly ice volume) and ostracod Mg:Ca ratios (bottomwater temperature). During glacial periods, H(S) = 0.2-0.6, whereas during interglacials, H(S) = 1.2-1.6, which is three to four times as high. The control of deep-sea benthic diversity by cyclic climate change at timescales of 103-104 yr does not support the stability-time hypothesis because it shows that the deep sea is a temporally dynamic environment. Diversity oscillations reflect large-scale response of the benthic community to climatically driven changes in either thermohaline circulation, bottom temperature (or temperature-related factors) and food, and a coupling of benthic diversity to surface productivity.

Nature

Negligible cycling of terrestrial carbon in many lakes of the arid circumpolar landscape

High-latitude environments store nearly half of the planet’s below-ground organic carbon (OC), mostly in perennially frozen permafrost soils. Climatic changes drive increased export of terrestrial OC into many aquatic networks, yet the role that circumpolar lakes play in mineralizing this carbon is unclear. Here we directly evaluate ecosystem-scale OC cycling for lakes of interior Alaska. This arid, low-relief lake landscape is representative of over a quarter of total northern circumpolar lake area, but is greatly under-represented in current studies. Contrary to projections based on work in other regions, the studied lakes had a negligible role in mineralizing terrestrial carbon; they received little OC from ancient permafrost soils, and had small net contribution to the watershed carbon balance. Instead, most lakes recycled large quantities of internally derived carbon fixed from atmospheric CO 2 , underscoring their importance as critical sites for material and energy provision to regional food webs. Our findings deviate from the prevailing paradigm that northern lakes are hotspots of terrestrial OC processing. The shallow and hydrologically disconnected nature of lakes in many arid circumpolar landscapes isolates them from terrestrial carbon processing under current climatic conditions.

Nature Geoscience

A climate trend analysis of Uganda

This brief report, drawing from a multi-year effort by the U.S. Agency for International Development (USAID) Famine Early Warning Systems Network (FEWS NET), identifies observed changes in rainfall and temperature in Uganda, based on an analysis of a quality-controlled, long time series of station observations throughout Uganda. Extending recent trends forward, it also provides a current and near-future context for understanding the actual nature of climate change impacts in the country, and a basis for identifying climate adaptations that may protect and improve the country's food security.

Fact Sheet

Building a state-space life cycle model for naturally produced Snake River fall Chinook salmon

In 1992, Snake River basin fall Chinook salmon (Oncorhynchus tshawytscha) were listed for protection under the U.S. Endangered Species Act (NMFS 1992) and the population remained below 1000 individuals until 2000. Since then, returns from natural production has rebounded to over 20,000 spawners owing to a host of factors including reduced harvest (Peters et al. 2001), stable minimum spawning flows (Groves and Chandler 1999), summer flow augmentation (Connor et al. 2003), predator control (Beamesderfer et al. 1996), hatchery supplementation (Rosenberger et al. 2017), improved juvenile passage structures (Adams et al. 2014), summer spill operations (Perry et al. 2006; Adams et al. 2008), and periods of favorable ocean conditions and food availability (Logerwell et al. 2003; Peterson et al. 2014). Given this change in abundance coincident with numerous management actions and fluctuation in environmental drivers, quantifying which factors contributed to the observed rebound in natural production can provide critical insights into future management actions for this at-risk population. Multistage life cycle models provide a powerful analytical framework for understating how each life stage of a population contributes to population growth rate (Moussalli and Hilborn 1986; Greene and Beechie 2004). Multistage models may also be used as an analytical framework to explicitly estimate demographic parameters of a population model. This approach has an advantage over single-stage stock-recruitment models by allowing population growth rates to be partitioned among life stages rather than aggregated over an entire life cycle. Such partitioning allows for estimating 1) stage-specific density dependence, and 2) stage-specific effects of environmental factors or management actions. For example, Zabel et al. (2006) estimated parameters of a multistage model used in the context of a population viability analysis for spring/summer Chinook salmon in the Snake River, but such an approach has yet to be applied to fall Chinook salmon in the Snake River basin. Typically, data informing estimates of abundance at particular “check points” in the life cycle determines the complexity of the multistage model that can be fit to the data. For fall Chinook salmon, we are developing a two-stage model that encompasses: 1) upstream passage of spawners at Lower Granite Dam (LGR) to the subsequent downstream passage of their progeny at the dam, and 2) downstream passage of juveniles at LGR to their subsequent return from the ocean and passage at the Dam 2‒6 years later. This approach partitions the life cycle of fall Chinook salmon both spatially and temporally, which allows us to fit and compare alternative models with covariates specific to each stage. Our previous report to the ISAB (Zabel et al. 2013) detailed methods for estimating abundance of naturally produced adults and juveniles passing Lower Granite Dam, which provides the requisite data for fitting a two-stage model. The intent of this report is to describe the structure of the two-stage life cycle model, present preliminary results from fitting the model to data, and outline future directions and developments. As is clear from the diversity of models presented in this report, “life cycle models” range from very simple theoretically based population models (e.g., the Beverton-Holt stock- recruitment model) to very complex spatially explicit simulation models linked to hydrosystem hydrodynamic models (e.g., the COMPASS model for a single transition in a life cycle model, Zabel et al. 2008). We chose to develop a model of intermediate complexity that casts the two- stage life cycle model in a state-space framework (Newman et al. 2014). We chose to use a state-space framework implemented in a Bayesian framework because: • It provides both a statistical estimation framework for retrospective statistical analysis and a stochastic simulation framework for prospective analysis to evaluate alternative management actions. • Abundance estimates are uncertain. A state-space framework accounts for observation uncertainty in the abundance estimates and other data (e.g., age structure) while simultaneously estimating process uncertainty. • It allows for missing data. By drawing missing data from an appropriate probability model, uncertainty owing to missing data can be propagated without having to omit data or assume fixed values for missing data. Thus, a two-stage state-space life cycle model for fall Chinook salmon strikes an appropriate balance between model complexity, tractability, and applicability given the goals of performing both retrospective and prospective analysis to guide future management of this population.

Idaho, Oregon, Washington, Wyoming

Population ecology and harvest of the American black duck: a review

1. The purpose of our review was to examine available data on population trends and current status of black ducks and trends in natality and survival and to relate these, where possible, to changes in habitat, predation, disease, contaminants, harvest, and hybridization with mallards. 2. The number of black ducks tallied in the winter survey has declined steadily over the past 30 years at an average rate of about 3%/ year. Reliability and precision of the survey are uncertain; it may not provide an adequate index to the continental population of black ducks. Breeding surveys are incomplete and sporadic, but black ducks have decreased in Ontario and increased in the Maritime Provinces and Quebec. 3. Recent declines in numbers of black ducks tallied in the winter survey are not unusual in magnitude or much different from those that have occurred among several other species of waterfowl. 4. At present, black ducks are not especially scarce relative to numbers of several other ducks in eastern North America. 5. There is no solid evidence of major decreases in quality or quantity of breeding habitat for black ducks in recent years; in some areas, habitat has improved. 6. Natural mortality of black ducks has not been well studied, but does not seem unusually high compared to other dabbling ducks. 7. Harvest rates of black ducks are similar to those of sympatric mallards as determined by banding analyses. 8. There is no strong evidence for direct effects of contaminants on black ducks, but some indirect effects through invertebrate food resources have been detected. 9. Age ratios in black ducks show no trend in the past 18 years. 10. The quality and quantity of wintering habitat for black ducks have decreased substantially in some areas. 11. Disease and other natural mortality that affect black ducks do .not occur in unusually high frequency. 12. A decline in harvest of black ducks has occurred; most of the decline has been in the United States, especially since restrictive regulations were implemented in 1983. 13. Recovery rates of black ducks have declined recently in the U.S., but not in Canada. 14. Survival rates of black ducks are 56-63% for adults and 43% for young. These rates of survival are similar to comparable estimates in sympatric mallards. 15. Long hunting seasons may depress survival in some sex-age classes of black ducks, buteffects of small reductions in survival on population trends are unknown. 16. Available evidence does not support the contention that hunting is either the sole or most important cause of the decline in the winter tally of black duck numbers. 17. Surveys and banding of black ducks should be thoroughly reviewed and maintained or improved as warranted. Obtaining or maintaining a reasonable index to numbers of black ducks is the top priority among survey needs. 18. Experimental manipulation of hunting seasons should be considered to elucidate relationships among regulations, harvest, survival, and population trends. 19. Black ducks and mallards are genetically similar; there is as much genetic differentiation within the 2 species as there is between them. 20. Black duck x mallard hybrids are fertile. Hybrids are difficult to detect by plumage and thus published frequencies (0-13%) of hybrids may be low. 21. Hybridization could be a result of concomitant mallard increases and black duck decreases, or changes in black duck-to-mallard ratios could be from hybridization and genetic swamping of black ducks.

Wildlife Society Bulletin

Walden Pond, Massachusetts: Environmental setting and current investigations

Introduction Walden Pond, in Concord, Massachusetts, is famous among lakes because of its unique social history. Walden was the setting for American naturalist Henry David Thoreau's well-known essay 'Walden; or, Life in the Woods,' first published in 1854. Thoreau lived and wrote at Walden Pond from July 1845 to September 1847. In 'Walden,' Thoreau combined highly admired writing on Transcendental philosophy with pioneering observations of aquatic ecology and physical aspects of limnology, the study of lakes. Because Thoreau also defended so effectively the value of living close to nature in the Walden woods, the pond is considered by many to be the birthplace of the American conservation movement. Visitors come from all over the world to the pond, which has been designated a National Historic Landmark, and its fame has resulted in a major fund drive to preserve the surrounding woods. Walden Pond has no surfacewater inflow or outflow, and much of its ground-water contributing area likely is preserved within the Walden Pond Reservation area (fig. 1). Only 15 miles from Boston, the pond is unusually clear and pristine for an urban-area lake. However, point sources of nutrients near the pond, and a large annual visitor attendance, concentrated during the summer when the swimming beach (fig. 2) is open, may contribute a nutrient load sufficient to change the pond environment. The occurrence of nuisance algal species, a recent beach closing, and an awareness of water-quality problems suffered by other ponds in the region raise concerns about the risk of ecological change at Walden Pond. Despite the role of Walden Pond as a cultural and environmental icon, little is known about the pond's ecological features, such as its internal nutrient cycling or the structure of its food web, nor have consistent measurements been made to determine whether these features are changing or are stable. Production rates of aquatic plants in lakes and ponds naturally undergo a slow increase as plant nutrients, organic matter, and soil are leached and eroded from the surrounding watershed. This process is known as eutrophication. The term 'cultural eutrophication' refers to an accelerated form of the natural process in which extra soil and nutrients are derived from people's use of fertilizer, rerouting of surface drainage, and disposal of domestic and industrial waste. Cultural eutrophication can lead to excessive growth of aquatic plants, pond filling by decayed plants and eroded soils, reduced water clarity, and depletion of dissolved oxygen in deep water with subsequent loss of cold-water fish habitat. In order to document the longterm ecological health of Walden Pond, the U.S. Geological Survey (USGS), working in cooperation with the Massachusetts Department of Environmental Management (MDEM), is investigating factors that could contribute to cultural eutrophication of Walden. Through measurements of mass balance of nutrients and oxygen in the pond's deep water, the investigation will establish a baseline data set on Walden's trophic state, which is a measure of the pond's ability to support plant growth. The baseline data will be used to detect trends and give early warning of trophic changes or trophic response to pond remediation projects. This Fact Sheet provides background information on the environmental setting, limnological features, and cultural eutrophication of Walden Pond, and describes the joint USGS/MDEM study.

Massachusetts

Trace elements and consequent ecological risks in mining- influenced streams of Appalachia

Appalachian coal surface mines fracture geologic materials, causing release of both major ions and trace elements to water via accelerated weathering. When elevated above natural background, trace elements in streams may produce adverse effects to biota via direct exposure from water and sediment and via dietary exposure in food sources. Other studies have found elevated water concentrations of multiple trace elements in Appalachia's mining-influenced streams. Excepting Se, trace-element concentrations in abiotic and biotic media of Appalachian mining-influenced streams are less well-known. We analyzed environmental media of headwater streams receiving alkaline waters from Appalachian coal mines for eight trace elements (Al, As, Cd, Cu, Ni, Sr, V, and Zn) and assessed potential consequent ecological risks. Streamwater, particulate media (sediment, biofilm, leaf detritus) and benthic macroinvertebrates (primary consumers, secondary consumers, crayfish) were sampled from six mining-influenced and three reference streams during low-flow conditions in two seasons. Dissolved Cu, Ni, and Sr were higher in mining-influenced streams than in reference streams; whereas Ni, Sr, and Zn in fine sediments and Ni in macroinvertebrates were also elevated relative to reference-stream levels in samples from mining-influenced streams. Seasonal ratios of mining-influenced stream concentrations to maximum concentrations in reference streams also demonstrated mining-influenced increases for several elements in multiple media. In most media, concentrations of several elements including Ni were correlated positively. All water-column dissolved concentrations were below protective levels, but fine-sediment concentrations of Ni approached or exceeded threshold-effect concentrations in several streams. Further study is warranted for several elements (Cd, Ni, and Zn in biofilms, and V in macroinvertebrates), which approached or exceeded previously established dietary-risk levels.

Virginia, West Virginia

Mass collecting and the diet breadth model: A Great Basin example

The energetic return rates of many small animal and plant resources are often density dependent. When these resources are collected in mass, change in abundance can dramatically affect diet rank, and challenges the assumption that return rates are generally correlated with body size. When mass collecting is employed, as a result of either natural events (e.g. windrows) or technological developments (e.g. nets), population density may largely determine the overall return rate for a resource. Since a single food or resource type can be many prey types, an increase in the abundance of a food resource can change its diet rank. We examined this relationship at Lakeside Cave in northwestern Utah, and discovered that when the abundance of grasshoppers is high, and mass collecting is productive, the hunting of bighorn sheep and other large animal resources may have been abandoned, contradicting commonly held assumptions about prey size. In archaeological situations it may be necessary to determine what foraging technique was used before assuming that the presence of small animals and fish in the diet is a result of reduced foraging efficiency.

Journal of Archaeological Science

Using noninvasive genetics for estimating density and assessing diet of urban and rural coyotes in Florida, USA

Coyotes ( Canis latrans ) are expanding their range and due to conflicts with the public and concerns of Coyotes affecting natural resources such as game or sensitive species, there is interest and often a demand to monitor Coyote populations. A challenge to monitoring is that traditional invasive methods involving live-capture of individual animals are costly and can be controversial. Natural resource management agencies can benefit from contemporary noninvasive genetic sampling approaches aimed at determining key aspects of Coyote ecology (e.g., population density and food habits). However, the efficacy of such approaches under different environmental conditions is poorly understood. Our objectives were to 1) examine accumulation and nuclear DNA degradation rates of Coyote scats in metropolitan and rural sites in Florida to help optimize methods to estimate population density; and 2) explore new genetic methods for determining diet of Coyotes based on vertebrate, plant, and invertebrate species DNA identified in scat. Recently developed DNA metabarcoding approaches make it possible to simultaneously identify DNA from multiple prey species in predator scat samples, but an exploration of this tool for assessing Coyote diet has not been pursued. We observed that scat accumulation rates (0.02 scats/km/day) did not vary between sites and fecal DNA amplification success decreased and genotyping errors increased over time with exposure to sun and precipitation. DNA sampling allowed us to generate a Coyote density estimate for the urban environment of eight Coyotes per 100 km2, but lack of recaptures in the rural area precluded density estimation. DNA metabarcoding showed promise for assessing diet contributions of vertebrate species to Coyote diet. Feral Swine (Sus scrofa) were detected as prey at higher frequencies than previously reported. We identify several considerations that can be used to optimize future noninvasive sampling efforts for Coyotes in the southeastern United States. We also discuss strengths and drawbacks of utilizing DNA metabarcoding for assessing diet of generalist carnivores such as Coyotes.

Florida

Isolation of methylmercury using distillation and anion-exchange chromatography for isotopic analyses in natural matrices

The development of mercury (Hg) stable isotope measurements has enhanced the study of Hg sources and transformations in the environment. As a result of the mixing of inorganic Hg (iHg) and methylmercury (MeHg) species within organisms of the aquatic food web, understanding species-specific Hg stable isotopic compositions is of significant importance. The lack of MeHg isotope measurements is due to the analytical difficulty in the separation of the MeHg from the total Hg pool, with only a few methods having been tested over the past decade with varying degrees of success, and only a handful of environmentally relevant measurements. Here, we present a novel anion-exchange resin separation method using AG 1-X4 that further isolates MeHg from the sample matrix, following a distillation pretreatment, in order to obtain ambient MeHg stable isotopic compositions. This method avoids the use of organic reagents, does not require complex instrumentation, and is applicable across matrices. Separation tests across sediment, water, and biotic matrices showed acceptable recoveries (98 ± 5%, n = 54) and reproducible δ 202 Hg isotope results (2 SDs ≤ 0.15‰) down to 5 ng of MeHg. The measured MeHg pools in natural matrices, such as plankton and sediments, showed large deviations from the non-speciated total Hg measurement, indicating that there is an important isotopic shift during methylation that is not recorded by typical measurements, but is vital in order to assess sources of Hg during bioaccumulation.

Analytical and Bioanalytical Chemistry