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Aquatic assemblages and their relation to temperature variables of least-disturbed streams in the Salmon River basin, central Idaho, 2001

In the late 1990s, Idaho’s established stream temperature criteria for the protection of coldwater biota and salmonid spawning were considered inadequate because the criteria did not agree with observed biological conditions in many instances and did not allow for variability in environmental condition or species diversity across a broad area such as the entire State of Idaho. In 2001, benthic invertebrate and fish assemblages in 34 least-disturbed streams in the Salmon River Basin, central Idaho, were evaluated in relation to stream temperature and other environmental variables. The Salmon River Basin retains watersheds that are minimally affected by human activities. These “natural” stream conditions provide a basis for deriving attainable stream temperatures that can be used to set new, and revise existing, water-quality criteria for stream habitats affected by human activities. During July through September 2001, data were collected to document the thermal regime of least-disturbed streams, characterize the distribution of aquatic biota at streams representing a gradient of temperature, and describe the relations between environmental variables and benthic invertebrate and fish assemblages. Nine stream temperature metrics were compared with the U.S. Environmental Protection Agency’s criterion of 10 ° C (degrees Celsius) for bull trout spawning and juvenile rearing. The maximum weekly-maximum temperature at all 33 sites where temperature data were available exceeded this criterion. The maximum daily-maximum temperature (MDMT) at 30 of the sites exceeded the Idaho Department of Environmental Quality (IDEQ) criterion of 13.0 ° C for the protection of salmonid spawning; and the maximum daily-average temperature at all 33 sites exceeded the 9.0 ° C criterion for the protection of salmonid spawning. The thermal regime at most sites did not exceed the IDEQ criteria for the protection of coldwater biota. Nine environmental variables—water-surface gradient, discharge, wetted channel width, width:depth ratio, aspect, total seasonal thermal input, open canopy, riparian canopy density, and elevation were selected for correlation with the nine stream temperature metrics. Elevation showed the strongest inverse correlation with the stream temperature metrics. Two hundred and one benthic invertebrate taxa from the 34 sampling sites were identified. The most abundant taxa were Baetis tricaudatus , Oligochaeta, Tvetenia bavarica gr., Acari, Rhithrogena , Cinygmula , Heterlimnius , Micropsectra , Eukiefferiella devonica gr., Drunella doddsi , and Cricotopus . Of the 201 benthic invertebrate taxa collected during this study, 57 taxa (present at a minimum of 5 sampling sites) were significantly correlated with one or more of the stream temperature metrics. Among the invertebrate taxa collected, 32 were identified as coldwater taxa. Of the coldwater taxa collected, Zapada oregonensis gr. showed the strongest inverse correlation with the stream temperature metrics and was collected at sites where maximum weekly-maximum temperature (based on date of sample and 6 days prior) ranged from 11.3 ° to 18.5 °C. Ten species of fish in the families Salmonidae, Cottidae, and Cyprinidae were collected. Two species (bull trout and chinook salmon) listed as threatened under the U.S. Fish and Wildlife Service Endangered Species Act were collected. Among all species, bull trout showed the strongest inverse correlation between relative fish abundance and stream temperature. Bull trout and juvenile bull trout densities were inversely correlated with stream temperature. The probability of occurrence of juvenile bull trout was significantly correlated with MDMT on the basis of results from a logistic regression model developed during this study. However, this model differed from a similar model developed by the U.S. Forest Service on the basis of regional data collected in the Pacific Northwest. The regression model based on data collected during this study showed higher probabilities of occurrence of bull trout at colder stream temperatures (10 ° to 15 ° C) and lower probabilities of occurrence at warmer stream temperatures (16 ° to 21 ° C) than did the model based on regional data. The model comparisons suggest that regional or local differences need to be considered when deriving stream temperature criteria.

Idaho

Handbook of acute toxicity of chemicals to fish and aquatic invertebrates : summaries of toxicity tests conducted at Columbia National Fisheries Research Laboratory, 1965-78

Acute toxicity is a major subject of research at Columbia National Fisheries Research Laboratory for evaluating the impact of toxic chemicals on fishery resources. The Laboratory has played a leading role in developing research technology for toxicity testing and data interpretation. In 1965-78, more than 400 chemicals were tested against a variety of invertebrates and fish species representative of both cold- and warm-water climates. The use of acute toxicity tests for assessing the potential hazard of chemical contaminants to aquatic organisms is well documented (Boyd 1957; Henderson et al. 1960; Sanders and Cope 1966; Macek and McAllister 1970). Static acute toxicity tests provide rapid and (within limits) reproducible concentration-response curves for estimating toxic effects of chemicals on aquatic organisms. These tests provide a database for determining relative toxicity of a large number of chemicals to a variety of species and for estimating acute effects of chemical spills on natural aquatic systems; they also assist in determining priority and design of additional toxicity studies. Acute toxicity tests usually provide estimates of the exposure concentration causing 50% mortality (LC50) to test organisms during a specified period of time. For certain invertebrates, the effective concentration is based on immobilization, or some other identifiable endpoint, rather than on lethality. The application of the LC50 has gained acceptance among toxicologists and is generally the most highly rated test for assessing potential adverse effects of chemical contaminants to aquatic life (Brungs and Mount 1978; American Institute for Biological Sciences 1978 a ). The literature contains numerous papers dealing with the acute toxicity of chemicals to freshwater organisms. However, there is a tremendous need for a concise compendium of toxicity data covering a large variety of chemicals and test species. This Handbook is a compilation of a large volume of acute toxicity data from the Columbia Laboratory and its field laboratories. It presents definitive acute toxicity data on 271 chemicals tested against a variety of freshwater invertebrates and fishes. The chemicals represent all major groups of pesticides, as well as numerous industrial chemicals. This compilation should serve as a useful database for the many agencies and organizations dealing with research and management programs concerned with the impact of chemicals on aquatic resources. The Columbia Laboratory has played a major role in developing currently used standard methodology for static acute toxicity testing. The use of standardized methodology greatly reduces variation in results. The data presented here have been carefully scrutinized to eliminate tests that failed to follow acceptable procedures. Handling of test organisms and procedures for static toxicity tests followed those described by Lennon and Walker (1964) and Macek and McAllister (1970), and conform well with those recommended by Brauhn and Schoettger (1975) and the Committee on Methods for Toxicity Tests with Aquatic Organisms (1975). The species of fish and invertebrates that were tested are listed in phylogenetic order in Tables 1 and 2. Fish were obtained from Federal and State hatcheries as either eggs or fry. Original stocks of invertebrates were collected and cultured from wild populations with no known source of contamination; these populations were replenished regularly. The invertebrates were cultured in the Laboratory by methods similar to those described by Sanders and Cope (1966). Test chemicals usually consisted of technical or analytical grade samples of known purity. Formulations of the chemicals were also tested when available. When purity of test chemicals was known, all calculated concentrations were based on percent active ingredients. Stock solutions were prepared immediately before each test, with commercial grade acetone as the carrier solvent. Occasionally, ethanol or dimethyl-formamide was substituted. Solvent concentrations did not exceed 0.5 mL/L in final dilution water. Test water (dilution water) was reconstituted from deionized water of at least 10 6 ohms resistivity by the addition of appropriate reagent grade chemicals (Marking 1969). Water was buffered to maintain a pH of 7.2 to 7.5, an alkalinity of 30 to 35 mg/L, and a hardness of 40 to 50 mg/L as CaCO 3 . Test water was mixed thoroughly and aerated before transfer into test chambers. Fish were acclimated to dilution water by gradually changing the water in acclimated tanks from 100% well water to 100% reconstituted water over a 1- to 3-day period at the desired testing temperature. Invertebrates were acclimated from well water to dilution water over a 4- to 6-h period. Toxicity tests were conducted under static conditions without aeration, and the organisms were not fed during acclimation or testing. Temperature of test solutions was maintained within ± 1°C of that required for a given test. Toxicity tests with fish were conducted in 18.9-liter (5-gal) wide-mouthed jars containing 15 liters of test solution. Fingerling fish weighing 0.2 to 1.5 g were tested at each concentration. Caution was taken not to exceed 0.8 g of test organisms per liter of solution. Duplicate test chambers were used to accommodate larger fish. Test chambers varied in size for invertebrates, depending on the species used; volume of test solution ranged from 0.25 to 4 liters. At least 10 organisms were exposed to each concentration for all definitive tests. At least six concentrations were used per toxicity test. The tests began upon initial exposure to the toxicant and continued for 96 h. Immobilization tests with invertebrates were conducted for only 48 h. The number of dead or affected organisms in each test chamber were recorded and the dead organisms were removed every 24 h; general observations on the condition of test organisms were also recorded at these times. Toxicity data were analyzed by a statistical method described by Litchfield and Wilcoxon (1949) to determine LC50 (theoretical estimate of the concentration lethal to 50% of the test animals) and 95% confidence intervals. This method is recommended by the American Public Health Association (1971) and by Sprague (1969) for determining median lethal concentrations. The procedure is easily modified for computing a single LC50 when replicate tests are performed.

Resource Publication

Relation of periphyton and benthic invertebrate communities to environmental factors and land use at selected sites in part of the upper Mississippi River basin, 1996-98

The Upper Mississippi River Basin is one of the hydrologic systems selected for study by the National Water-Quality Assessment (NAWQA) Program of the U.S. Geological Survey. NAWQA utilizes a multi-disciplinary approach to explain factors that affect water quality. Part of the NAWQA design addresses the relation of land use and environmental factors to periphyton and benthic invertebrate communities in streams. This report focuses on a 122,000 square kilometer area of the Mississippi River Basin, including the Twin Cities metropolitan area (TCMA). The northeastern part of the study area is forested, the southwestern part is agricultural, and the central part is transitional between forest and agriculture. Sampling sites were selected based on a process that identified small streams in predominantly forested, agricultural, and urban settings, and large river sites on the Mississippi River and major tributaries. Periphyton and benthic invertebrate communities were evaluated at each site. Compared to the forested site, periphyton density and biovolume in small streams generally increased as nutrient concentrations associated with urban and agricultural land use increased. Periphyton communities varied within agricultural and urban streams, indicating that physical and chemical factors other than land use also affect periphyton communities. Benthic invertebrate communities also are affected by land use and associated stream habitat. There were few intolerant taxa (Ephemeroptera and Plecoptera) in urban streams, potentially due to high streamflow variability and contaminants from runoff. Ephemeroptera taxa richness was greatest in the agricultural streams. The most abundant Ephemeroptera taxa were those tolerant to high concentrations of suspended sediment. Richness of Plecoptera and Trichoptera taxa were greatest in the forested stream. Biological communities in the St. Croix and Minnesota River generally reflected relatively homogeneous land uses. Biological communities in the Mississippi River reflected changes in water quality and physical habitat as the Minnesota and St. Croix Rivers join the Mississippi River. Periphyton density and biovolume, and the relative abundance of blue-green algae density increased in the Mississippi River at the confluence compared to the Minnesota and St. Croix Rivers. Relative abundance of benthic invertebrate taxa richness and diversity generally decreased downstream in the large rivers as urban and agricultural land use become more prevalent. Impoundments and dredging of the Mississippi River in and downstream from the TCMA exacerbate effects of increasing river size to produce a more lake-like system.

Iowa, Minnesota, North Dakota, South Dakota, Wisco

Water-quality assessment of Rattlesnake Creek watershed, Ohio

Chemical and biological water quality in Rattlesnake Creek basin, Ohio, are evaluated. The data include field and laboratory data for eight sites during August 1976- August 1977 and summaries of earlier (1972-76) data. Streamflow was below normal during the study period. Basin waters types were calcium bicarbonate or calcium magnesium bicarbonate. Specific conductance ranged from 405 to 1,300 micromhos per centimeter. High concentrations of sodium (110-140 milligrams per liter) , nitrogen (24 milligrams per liter as N), and phosphorus (7.8 milligrams per liter as P) were observed during low flows downstream from domestic sewage facilities. Nonpoint sources contributed high concentrations of nitrate-nitrogen to all streams during the high flows of winter and spring. Dissolved-oxygen concentrations in the upper basin ranged from 3.2 to 18.4 milligrams per liter. Mean saturation values of dissolved oxygen were at or near 100 percent in the lower basin. Stream pH exceeded 8.4 when dissolved-oxygen saturation was above 120 percent. Bacteria and invertebrate data suggest that moderate pollution from cultural sources may exist in the upper basin. Water quality was poorest in the sluggish flows of the upper basin but improved downstream. Increases in flow velocity and stream aeration rates, dilution, and biological activity contributed to the downstream recovery. Except for' high nitrogen concentrations, water quality was best in the lower basin.

Ohio

Biological, habitat, and water quality conditions in the upper Merced River drainage, Yosemite National Park, California, 1993-1996

Four studies were done in the upper Merced River drainage in Yosemite National Park and nearby areas from 1993 to 1996. First, monitoring studies of benthic algae, benthic invertebrates, fish, and habitat were undertaken at sites near Happy Isles and Pohono bridges from 1993 to 1995 as part of the National Water-Quality Assessment Program of the U.S. Geological Survey. Second, an ecological survey of benthic algae, benthic invertebrates, fish, and habitat was done in the upper Merced River drainage in 1994. Third, a special study of benthic algae, habitat, and water quality was done in the reach of the Merced River within Yosemite Valley to deter-mine whether human activities were having measurable effects on the ecosystem. Fourth, baseline data on benthic algae, benthic invertebrates, and habitat were collected in 1996 at four sites, two of which were undergoing extensive streambank restoration activities. Comparisons of the baseline data with future collections could be used to assess the effects of streambank restoration on aquatic biota. The general conclusion from these studies is that water quality in the upper Merced River was very good from 1993-1996, despite high levels of human activities in some areas. Fish communities did not appear to be a useful indicator of habitat and water quality because of low species richness and the apparent importance of physical barriers in determining species distributions. Measurements of fish densities and size-distributions might be useful, but would be logistically difficult. Benthic algae and benthic invertebrates do appear to be useful in monitoring environmental conditions. Benthic algae may be more sensitive than benthic invertebrates to small environmental differences within years. Benthic algae were also more responsive than benthic invertebrates to differences in discharge between years. Thus, benthic invertebrates may be more useful in comparing environmental conditions between years, independent of discharge conditions.

Water-Resources Investigations Report

Ecosystem ecology meets adaptive management: food web response to a controlled flood on the Colorado River, Glen Canyon

Large dams have been constructed on rivers to meet human demands for water, electricity, navigation, and recreation. As a consequence, flow and temperature regimes have been altered, strongly affecting river food webs and ecosystem processes. Experimental high‐flow dam releases, i.e., controlled floods, have been implemented on the Colorado River, USA, in an effort to reestablish pulsed flood events, redistribute sediments, improve conditions for native fishes, and increase understanding of how dam operations affect physical and biological processes. We quantified secondary production and organic matter flows in the food web below Glen Canyon dam for two years prior and one year after an experimental controlled flood in March 2008. Invertebrate biomass and secondary production declined significantly following the flood (total biomass, 55% decline; total production, 56% decline), with most of the decline driven by reductions in two nonnative invertebrate taxa, Potamopyrgus antipodarum and Gammarus lacustris . Diatoms dominated the trophic basis of invertebrate production before and after the controlled flood, and the largest organic matter flows were from diatoms to the three most productive invertebrate taxa ( P. antipodarum , G. lacustris , and Tubificida). In contrast to invertebrates, production of rainbow trout ( Oncorhynchus mykiss ) increased substantially (194%) following the flood, despite the large decline in total secondary production of the invertebrate assemblage. This counterintuitive result is reconciled by a post‐flood increase in production and drift concentrations of select invertebrate prey (i.e., Chironomidae and Simuliidae) that supported a large proportion of trout production but had relatively low secondary production. In addition, interaction strengths, measured as species impact values, were strongest between rainbow trout and these two taxa before and after the flood, demonstrating that the dominant consumer–resource interactions were not necessarily congruent with the dominant organic matter flows. Our study illustrates the value of detailed food web analysis for elucidating pathways by which dam management may alter production and strengths of species interactions in river food webs. We suggest that controlled floods may increase production of nonnative rainbow trout, and this information can be used to help guide future dam management decisions.

Arizona

Coastal groundwater/surface-water interactions: a Great Lakes case study

Key similarities exist between marine and Great Lakes coastal environments. Water and nutrient fluxes across lakebeds in the Great Lakes are influenced by seiche and wind set-up and set-down, analogous to tidal influence in marine settings. Groundwater/surface-water interactions also commonly involve a saline-fresh water interface, although in the Great-Lakes cases, it is groundwater that is commonly saline and surface water that is fresh. Evapotranspiration also affects nearshore hydrology in both settings. Interactions between groundwater and surface water have recently been identified as an important component of ecological processes in the Great Lakes. Water withdrawals and the reversal of the groundwater/surface water seepage gradient are also common to many coastal areas around the Great Lakes. As compared to surface water, regional groundwater that discharges to western Lake Erie from Michigan is highly mineralized. Studies conducted by the U.S. Geological Survey at Erie State Game Area in southeastern Michigan, describe groundwater flow dynamics and chemistry, shallow lake-water chemistry, and fish and invertebrate communities. Results presented here provide an overview of recent progress of ongoing interdisciplinary studies of Great Lakes nearshore systems and describe a conceptual model that identifies relations among geologic, hydrologic, chemical, and biological processes in the coastal habitats of Lake Erie. This conceptual model is based on analysis of hydraulic head in piezometers at the study site and chemical analysis of deep and shallow coastal groundwater.

Book chapter

2010-2015 Juvenile fish ecology in the Nisqually River Delta and Nisqually Reach Aquatic Reserve

The return of tidal inundation to over 750 acres of the U. S. Fish and Wildlife Service Billy Frank Jr. Nisqually National Wildlife Refuge (NNWR) in fall of 2009 was the crowning moment in the effort to protect and restore the Nisqually Delta. The Nisqually NWR project complemented three earlier restoration projects completed by the Nisqually Indian Tribe (Tribe) on tribal property to restore over 900 acres of the estuary, representing the largest estuary restoration project in the Pacific Northwest and one of the most significant advances to date towards the recovery of Puget Sound (USFWS 2005). In 2011 the Washington Department of Natural Resources (WADNR established the over 14000 acre Nisqually Reach Aquatic Reserve (Reserve), complementing the protection and restoration successes in the Nisqually Delta. The Reserve includes all state-owned aquatic lands around Anderson, Ketron and Eagle islands and part of McNeil Island (Figure 1, WDNR 2011). The Reserve also includes a diverse assemblage of nearshore and offshore habitats important to resident and migratory fish including federal endangered species act listed fish like Chinook salmon (Oncorynchus tshawytscha) and steelhead (O. mykiss). Studies in the Nisqually Estuary (Ellings and Hodgson 2007, David et al. 2014, Ellings et al. 2016) and South Puget Sound (Duffy 2003) have summarized fish use of the area. However, the fish ecology of the reserve had not been systematically surveyed. The Tribe, U.S. Geological Survey (USGS), NNWR, Nisqually River Foundation (NRF), and others are currently conducting a multi-year, interdisciplinary, hypothesis-based research and monitoring study investigating the impact of delta restoration on estuarine processes, habitat structures, and functions. Our interdisciplinary monitoring framework enables us to link key estuarine processes with habitat development and biological response at multiple scales across the restored footprint, reference marshes, and throughout the Nisqually Reach. Key research components include hydrology and sediment regime, channel and marsh topography and development, vegetation colonization, and invertebrate, bird, and fish abundance, habitat use, and foraging ecology. After the Reserve was established, the WDNR and the research partnership led by the Tribe expanded the existing delta fish ecology assessment to include sampling stations throughout the Reserve. The results of the Reserve fish ecology assessment provide a unique regional analysis of fish ecology from the Nisqually River to McNeil Island.

Salmon Recovery Program Technical Report

Geospatial characteristics of Florida's coastal and offshore environments: Distribution of important habitats for coastal and offshore biological resources and offshore sand resources

The Geospatial Characteristics GeoPDF of Florida's Coastal and Offshore Environments is a comprehensive collection of geospatial data describing the political boundaries and natural resources of Florida. This interactive map provides spatial information on bathymetry, sand resources, and locations of important habitats (for example, Essential Fish Habitats (EFH), nesting areas, strandings) for marine invertebrates, fish, reptiles, birds, and marine mammals. The map should be useful to coastal resource managers and others interested in marine habitats and submerged obstructions of Florida's coastal region. In particular, as oil and gas explorations continue to expand, the map can be used to explore information regarding sensitive areas and resources in the State of Florida. Users of this geospatial database will have access to synthesized information in a variety of scientific disciplines concerning Florida's coastal zone. This powerful tool provides a one-stop assembly of data that can be tailored to fit the needs of many natural resource managers. The map was originally developed to assist the Bureau of Ocean Energy Management, Regulation, and Enforcement (BOEMRE) and coastal resources managers with planning beach restoration projects. The BOEMRE uses a systematic approach in planning the development of submerged lands of the Continental Shelf seaward of Florida's territorial waters. Such development could affect the environment. BOEMRE is required to ascertain the existing physical, biological, and socioeconomic conditions of the submerged lands and estimate the impact of developing these lands. Data sources included the National Oceanic and Atmospheric Administration, BOEMRE, Florida Department of Environmental Protection, Florida Geographic Data Library, Florida Fish and Wildlife Conservation Commission, Florida Natural Areas Inventory, and the State of Florida, Bureau of Archeological Research. Federal Geographic Data Committee (FGDC) compliant metadata are provided as attached xml files for all geographic information system (GIS) layers.

Florida

Benthic habitats and offshore geological resources of Kaloko-Honokohau National Historical Park, Hawai‘i

Kaloko-Honokōhau National Historical Park (KAHO) is one of three National Park lands located along the western coast of the Island of Hawai‘i and the only one to include submerged lands and marine resources within its official boundaries. The park was established in 1978 and is 1,160 acres in size, including 596 acres of marine area. The submerged lands are currently managed by the State of Hawaii, Department of Land and Natural Resources, Division of Aquatic Resources (DLNR-DAR). Marine resources located within KAHO include coral reef and habitat for many marine animals, including the green sea turtle and a variety of fish and invertebrates. In addition, many archeological, cultural, and recreational resources are located within the marine realm of the park. Potential threats and stressors to the modern marine environment include ground-water and surface-water contamination, invasive plants and algae, fishing pressure and use of monofilament gill nets (which can ensnare marine life or become tangled on reefs and be left behind as fishing debris), and visitor use impacts, such as scuba diving and snorkeling. Illegal dumping, oil releases, boat groundings, and other physical damage to reef resources are potential threats from users of the nearby harbor. Special issues of concern for the park include establishing baseline conditions of the offshore resources before the development of adjacent coastal lands. Until this study, only a general knowledge of the distribution of benthic habitats and the characteristics of the offshore region of Kaloko-Honokōhau National Historical Park was available. In 2003, a collaborative project between the U.S. Geological Survey (USGS) Coastal and Marine Geology Program and the National Park Service (NPS) was initiated to develop detailed benthic-habitat classification maps for the marine lands within and adjacent to the park. The intent of this project is to provide baseline maps and a Geographic Information System (GIS) database and description of the biological and geological resources of these marine lands in order to facilitate the management, interpretation, and understanding of park resources. A benthic-habitat classification map was created for the park using existing color aerial photography, Scanning Hydrographic Operational Airborne Lidar Survey (SHOALS) bathymetric data, georeferenced underwater video, and still photography. Individual habitat polygons were classified using five basic attributes: (1) major structure or substrate, (2) dominant structure, (3) major biologic cover on the substrate, (4) percentage of major biological cover, and (5) geographic zone. Additional information regarding geology, morphology, and coral species were also noted. Marine resources located within KAHO include coral reef and habitat for many marine animals, including the green sea turtle and a variety of fish and invertebrates. In addition, many archeological, cultural, and recreational resources are located within the marine realm of the park. Potential threats and stressors to the modern marine environment include ground-water and surface-water contamination, invasive plants and algae, fishing pressure and use of monofilament gill nets (which can ensnare marine life or become tangled on reefs and be left behind as fishing debris), and visitor use impacts, such as scuba diving and snorkeling. Illegal dumping, oil releases, boat groundings, and other physical damage to reef resources are potential threats from users of the nearby harbor. Special issues of concern for the park include establishing baseline conditions of the offshore resources before the development of adjacent coastal lands. Until this study, only a general knowledge of the distribution of benthic habitats and the characteristics of the offshore region of Kaloko-Honokōhau National Historical Park was available. In 2003, a collaborative project between the U.S. Geological Survey (USGS) Coastal and Marine Geology Program and the National Park Service (NPS) was initiated to develop detailed benthic-habitat classification maps for the marine lands within and adjacent to the park. The intent of this project is to provide baseline maps and a Geographic Information System (GIS) database and description of the biological and geological resources of these marine lands in order to facilitate the management, interpretation, and understanding of park resources. A benthic-habitat classification map was created for the park using existing color aerial photography, Scanning Hydrographic Operational Airborne Lidar Survey (SHOALS) bathymetric data, georeferenced underwater video, and still photography. Individual habitat polygons were classified using five basic attributes: (1) major structure or substrate, (2) dominant structure, (3) major biologic cover on the substrate, (4) percentage of major biological cover, and (5) geographic zone. Additional information regarding geology, morphology, and coral species were also noted.

Hawai'i

Vascular Plant and Vertebrate Inventory of Tumacacori National Historical Park

Executive Summary This report summarizes the results of the first comprehensive biological inventory of Tumacacori National Historical Park (NHP) in southern Arizona. These surveys were part of a larger effort to inventory vascular plants and vertebrates in eight National Park Service units in Arizona and New Mexico. From 2000 to 2003 we surveyed for vascular plants and vertebrates (fish, amphibians, reptiles, birds, and mammals) at Tumacacori NHP to document presence of species within the administrative boundaries of the park's three units. Because we used repeatable study designs and standardized field techniques, these inventories can serve as the first step in a long-term monitoring program. We recorded 591 species at Tumacacori NHP, significantly increasing the number of known species for the park (Table 1). Species of note in each taxonomic group include: * Plants: second record in Arizona of muster John Henry, a non-native species that is ranked a 'Class A noxious weed' in California; * Amphibian: Great Plains narrow-mouthed toad; * Reptiles: eastern fence lizard and Sonoran mud turtle; * Birds: yellow-billed cuckoo, green kingfisher, and one observation of the endangered southwestern willow flycatcher; * Fishes: four native species including an important population of the endangered Gila topminnow in the Tumacacori Channel; * Mammals: black bear and all four species of skunk known to occur in Arizona. We recorded 79 non-native species (Table E.S.1), many of which are of management concern, including: Bermudagrass, tamarisk, western mosquitofish, largemouth bass, bluegill, sunfish, American bullfrog, feral cats and dogs, and cattle. We also noted an abundance of crayfish (a non-native invertebrate). We review some of the important non-native species and make recommendations to remove them or to minimize their impacts on the native biota of the park. Based on the observed species richness, Tumacacori NHP possesses high biological diversity of plants, fish, and birds for a park of its size. This richness is due in part to the ecotone between ecological provinces (Madrean and Sonoran), the geographic distribution of the three units (23 km separates the most distant units), and their close proximity to the Santa Cruz River. The mesic life zone along the river, including rare cottonwood/willow forests and adjacent mesquite bosque at the Tumacacori unit, is representative of areas that have been destroyed or degraded in many other locations in the region. Additional elements such as the semi-desert grassland vegetation community are also related to high species richness for some taxonomic groups. This report includes lists of species recorded by us (or likely to be recorded with additional effort) and maps of study sites. We also suggest management implications and ways to maintain or enhance the unique biological resources of Tumacacori NHP: limit development adjacent to the park, exclude cattle and off-road vehicles, develop an eradication plan for non-native species, and hire a natural resource specialist. These recommendations are intended to assist park staff with addressing many of the goals set out in their most recent natural resources management plan. This study is the first step in a long-term process of compiling information on the biological resources of Tumacacori NHP and its surrounding areas, and our findings should not be viewed as the final authority on the plants and animals of the park. Therefore, we also recommend additional inventory and monitoring studies and identify components of our effort that could be improved upon, either through the application of new techniques (e.g., use of genetic markers) or by extending the temporal and/or spatial scope of our research.

Open-File Report

Characterization of ecological risks at the Milltown Reservoir-Clark Fork River Sediments Superfund Site, Montana

A comprehensive field and laboratory approach to the ecological risk assessment for the Milltown Reservoir-Clark Fork River Sediments Site, a Superfund site in the Rocky Mountains of Montana, has been described in the preceding reports of this series. The risk assessment addresses concerns over the ecological impacts of upstream releases of mining wastes to fisheries of the upper Clark Fork River (CFR) and the benthic and terrestrial habitats further downstream in Milltown Reservoir. The risk characterization component of the process integrated results from a triad of information sources: (a) chemistry studies of environmental media to identify and quantify exposures of terrestrial and aquatic organisms to site-related contaminants; (b) ecological or population studies of terrestrial vegetation, birds, benthic communities, and fish; and (c) in situ and laboratory toxicity studies with terrestrial and aquatic invertebrates and plants, small mammals, amphibians, and fish exposed to contaminated surface water, sediments, wetland soils, and food sources. Trophic transfer studies were performed on waterfowl, mammals, and predatory birds using field measurement data on metals concentrations in environmental media and lower trophic food sources. Studies with sediment exposures were incorporated into the Sediment Quality Triad approach to evaluate risks to benthic ecology. Overall results of the wetland and terrestrial studies suggested that acute adverse biological effects were largely absent from the wetland; however, adverse effects to reproductive, growth, and physiological end points of various terrestrial and aquatic species were related to metals exposures in more highly contaminated depositional areas. Feeding studies with contaminated diet collected from the upper CFR indicated that trout are at high risk from elevated metals concentrations in surface water, sediment, and aquatic invertebrates. Integration of chemical analyses with toxicological and ecological evaluations of metal effects on the wetland and fishery has provided an important foundation for environmental decisions at this site.

Montana

Mixed-chemical exposure and predicted effects potential in wadeable southeastern USA streams

Complex chemical mixtures have been widely reported in larger streams but relatively little work has been done to characterize them and assess their potential effects in headwaterstreams. In 2014, the United States Geological Survey (USGS) sampled 54 Piedmont streams over ten weeks and measured 475 unique organic compounds using five analytical methods. Maximum and median exposure conditions were evaluated in relation to watershed characteristics and for potential biological effects using multiple lines of evidence. Results demonstrate that mixed-contaminant exposures are ubiquitous and varied in sampled headwater streams. Approximately 56% (264) of the 475 compounds were detected at least once across all sites. Cumulative maximum concentrations ranged 1,922–162,346 ng L −1 per site. Chemical occurrence significantly correlated to urban land use but was not related to presence/absence of wastewater treatment facility discharges. Designed bioactive chemicals represent about 2/3rd of chemicals detected, notably pharmaceuticals and pesticides, qualitative evidence for possible adverse biological effects. Comparative Toxicogenomics Database chemical-gene associations applied to maximum exposure conditions indicate >12,000 and 2,900 potential gene targets were predicted at least once across all sites for fish and invertebrates, respectively. Analysis of cumulative exposure-activity ratios provided additional evidence that, at a minimum, transient exposures with high probability of molecular effects to vertebrates were common. Finally, cumulative detections and concentrations correlated inversely with invertebrate metrics from in-stream surveys. The results demonstrate widespread instream exposure to extensive contaminant mixtures and compelling multiple lines of evidence for adverse effects on aquatic communities.

Science of the Total Environment

Water-quality and biological conditions in selected tributaries of the Lower Boise River, southwestern Idaho, water years 2009-12

Water-quality conditions were studied in selected tributaries of the lower Boise River during water years 2009–12, including Fivemile and Tenmile Creeks in 2009, Indian Creek in 2010, and Mason Creek in 2011 and 2012. Biological samples, including periphyton biomass and chlorophyll-a, benthic macroinvertebrates, and fish were collected in Mason Creek in October 2011. Synoptic water-quality sampling events were timed to coincide with the beginning and middle of the irrigation season as well as the non-irrigation season, and showed that land uses and irrigation practices affect water quality in the selected tributaries. Large increases in nutrient and sediment concentrations and loads occurred over relatively short stream reaches and affected nutrient and sediment concentrations downstream of those reaches. Escherichia coli (E. coli) values increased in study reaches adjacent to pastured lands or wastewater treatment plants, but increased E. coli values at upstream locations did not necessarily affect E. coli values at downstream locations. A spatial loading analysis identified source areas for nutrients, sediment, and E. coli , and might be useful in selecting locations for water-quality improvement projects. Effluent from wastewater treatment plants increased nutrient loads in specific reaches in Fivemile and Indian Creeks. Increased suspended-sediment loads were associated with increased discharge from irrigation returns in each of the studied tributaries. Samples collected during or shortly after storms showed that surface runoff, particularly during the winter, may be an important source of nutrients in tributary watersheds with substantial agricultural land use. Concentrations of total phosphorus, suspended sediment, and E. coli exceeded regulatory water-quality targets or trigger levels at one or more monitoring sites in each tributary studied, and exceedences occurred during irrigation season more often than during non-irrigation season. As with water-quality sampling results, bottom-sediment samples analyzed for contaminants of emerging concern indicated that adjacent land uses can affect in-stream conditions. Contaminants of emerging concern were detected in four categories: urban compounds, industrial compounds, fecal steroids, and personal care products. Compounds in one or more of the four contaminant categories were detected at higher concentrations in upstream sites than in downstream sites in the tributaries and in the lower Boise River. High concentrations of compounds in upstream locations indicated that adjacent land use might be an important factor in contributing contaminants of emerging concern to the lower Boise River watershed. Expanded monitoring at Mason Creek near the mouth included a streamgage, a continuous water-quality monitor, and monthly water-quality sample collection. Data collected during expanded monitoring efforts at Mason Creek near the mouth provided information to develop and compare water-quality models. Regression models were developed using turbidity, discharge, and seasonality as surrogates to estimate concentrations of water-quality constituents. Daily streamflow also was used in a load model to estimate daily loads of water-quality constituents. Surrogate regression models may be useful for long-term monitoring and generally performed better than other models to estimate concentrations and loads of total phosphorus, total nitrogen, and suspended sediment in Mason Creek. Biological sampling results from Mason Creek showed low periphyton biomass and chlorophyll-a concentrations compared to those historically measured in the Boise River near Parma, Idaho, during October and November. The most abundant invertebrate found in Mason Creek was the highly tolerant and invasive New Zealand mudsnail ( Potamopyrgus antipodarum ). The presence of small rainbow trout (90 millimeters) may indicate salmonid spawning in Mason Creek. The rangeland-fish-index score of 58 for Mason Creek is comparable to rangeland-fish-index scores calculated for the Boise River near Middleton, indicating intermediate biotic condition.

Idaho

Methods for collection and analysis of aquatic biological and microbiological samples

Chapter A4 contains methods used by the U.S. Geological Survey to collect, preserve, and analyze waters to determine their biological and microbiological properties. Part 1 discusses biological sampling and sampling statistics. The statistical procedures are accompanied by examples. Part 2 consists of detailed descriptions of more than 45 individual methods, including those for bacteria, phytoplankton, zooplankton, seston, periphyton, macrophytes, benthic invertebrates, fish and other vertebrates, cellular contents, productivity, and bioassays. Each method is summarized, and the application, interferences, apparatus, reagents, collection, analysis, calculations, reporting of results, precision and references are given. Part 3 consists of a glossary. Part 4 is a list of taxonomic references.

Techniques of Water-Resources Investigations

Biological communities in San Francisco Bay track large‐scale climate forcing over the North Pacific

Long‐term observations show that fish and plankton populations in the ocean fluctuate in synchrony with large‐scale climate patterns, but similar evidence is lacking for estuaries because of shorter observational records. Marine fish and invertebrates have been sampled in San Francisco Bay since 1980 and exhibit large, unexplained population changes including record‐high abundances of common species after 1999. Our analysis shows that populations of demersal fish, crabs and shrimp covary with the Pacific Decadal Oscillation (PDO) and North Pacific Gyre Oscillation (NPGO), both of which reversed signs in 1999. A time series model forced by the atmospheric driver of NPGO accounts for two‐thirds of the variability in the first principal component of species abundances, and generalized linear models forced by PDO and NPGO account for most of the annual variability of individual species. We infer that synchronous shifts in climate patterns and community variability in San Francisco Bay are related to changes in oceanic wind forcing that modify coastal currents, upwelling intensity, surface temperature, and their influence on recruitment of marine species that utilize estuaries as nursery habitat. Ecological forecasts of estuarine responses to climate change must therefore consider how altered patterns of atmospheric forcing across ocean basins influence coastal oceanography as well as watershed hydrology.

California

A multi-metric assessment of environmental contaminant exposure and effects in an urbanized reach of the Charles River near Watertown, Massachusetts

The Charles River Project provided an opportunity to simultaneously deploy a combination of biomonitoring techniques routinely used by the U.S. Geological Survey National Water Quality Assessment Program, the Biomonitoring of Environmental Status and Trends Project, and the Contaminant Biology Program at an urban site suspected to be contaminated with polycyclic aromatic hydrocarbons. In addition to these standardized methods, additional techniques were used to further elucidate contaminant exposure and potential impacts of exposure on biota. The purpose of the study was to generate a comprehensive, multi-metric data set to support assessment of contaminant exposure and effects at the site. Furthermore, the data set could be assessed to determine the relative performance of the standardized method suites typically used by the National Water Quality Assessment Program and the Biomonitoring of Environmental Status and Trends Project, as well as the additional biomonitoring methods used in the study to demonstrate ecological effects of contaminant exposure. The Contaminant Effects Workgroup, an advisory committee of the U.S. Geological Survey/Contaminant Biology Program, identified polycyclic aromatic hydrocarbons as the contaminant class of greatest concern in urban streams of all sizes. The reach of the Charles River near Watertown, Massachusetts, was selected as the site for this study based on the suspected presence of polycyclic aromatic hydrocarbon contamination and the presence of common carp ( Cyprinus carpio ), largemouth bass ( Micropterus salmoides ), and white sucker ( Catostomus commersoni ). All of these fish have extensive contaminant-exposure profiles related to polycyclic aromatic hydrocarbons and other environmental contaminants. This project represented a collaboration of universities, Department of the Interior bureaus including multiple components of the USGS (Biological Resources Discipline and Water Resources Discipline Science Centers, the Contaminant Biology Program, and the Status and Trends of Biological Resources Program), and the U.S. Fish and Wildlife Service. Samples for analyzing water chemistry, sediment chemistry and toxicity, fish community structure, tissue chemistry, and fish (20 carp, 20 bass, and 40 white sucker) and invertebrate pathology were collected in late August, 2005. This report provides results from the analyses of fish pathology, biomarkers of exposure and effects (reproductive, carcinogenic, genotoxic, and immunologic), sediment chemistry, toxicity, and fish and invertebrate community structure.

Massachusetts

Reconnaissance investigation of water quality, bottom sediment, and biota associated with irrigation drainage in the Vermejo Project area and the Maxwell National Wildlife Refuge, Colfax County, northeastern New Mexico, 1993

Based on findings of limited studies during 1989-92, a reconnaissance investigation was conducted in 1993 to assess the effects of the Vermejo Irrigation Project on water quality in the area of the project, including the Maxwell National Wildlife Refuge. This project was part of a U.S. Department of the Interior National Irrigation Water-Quality Program to determine whether irrigation drainage has caused or has the potential to cause significant harmful effects on human health, fish, and wildlife and whether irrigation drainage may adversely affect the suitability of water for other beneficial uses. For this study, samples of water, sediment, and biota were collected from 16 sites in and around the Vermejo Irrigation Project prior to, during the latter part of, and after the 1993 irrigation season (April, August-September, and November, respectively). No inorganic constituents exceeded U.S. Environmental Protection Agency drinking-water standards. The State of New Mexico standard of 750 micrograms per liter for boron in irrigation water was exceeded at three sites (five samples), though none exceeded the livestock water standard of 5,000 micrograms per liter. Selenium concentrations exceeded the State of New Mexico chronic standard of 2 micrograms per liter for wildlife and fisheries water in at least eight samples from five sites. Bottom-sediment samples were collected and analyzed for trace elements and compared to concentrations of trace elements in soils of the Western United States. Concentrations of three trace elements at eight sites exceeded the upper values of the expected 95-percent ranges for Western U.S. soils. These included molybdenum at one site, selenium at seven sites, and uranium at four sites. Cadmium and copper concentrations exceeded the National Contaminant Biomonitoring Program 85th percentile in fish from six sites. Average concentrations of selenium in adult brine flies (33.7 μ g/g dry weight) were elevated above concentrations in other invertebrates. Concentrations of other elements were below their respective toxicity levels. Plants, invertebrates, fish, and fish fillets were collected and analyzed. These analyses were compared to diagnostic criteria and to each other to determine the extent of bioaccumulation of trace elements. Plants contained larger dry weight concentrations of aluminum, arsenic, boron, chromium, iron, lead, magnesium, manganese, nickel, and vanadium than invertebrates and fish. Adult brine flies, gathered from playas, contained larger geometric mean dry weight concentrations of boron, magnesium, and selenium than other invertebrates. Of all samples collected, the largest mercury concentrations were found in fish fillets, although these concentrations were below levels of concern. Mercury and selenium bioaccumulation was evident in various habitats of the study area. Biological samples from Natural playa, an endemic wetland, and Half playa, a playa that receives additional water through seepage and irrigation delivery canals, generally had elevated concentrations of boron, iron, magnesium, and selenium than samples from reservoir and river sites. Selenium concentrations were lowest in biota from the two reservoir sites, although a wetland immediately downstream from the dam impounding Lake No. 13 (created by seepage from the reservoir) had elevated concentrations of selenium in biota. The geometric mean selenium concentration of whole-fish samples, except those from Lakes No. 13 and No. 14, exceeded the 5- μ g/g dry weight selenium concentration that demarcates the approximate lower limit of the threshold range of concentrations that have freen associated with adverse effects on piscine reproduction. Biota collected on and in the area around Maxwell National Wildlife Refuge contained concentrations of selenium that are in the lower range of threshold values that have been associated (dietarily) with risks of avian reproductive abnormalities. Bottom-sediment samples from two sites downstream from the irrigation project were collected and analyzed for 23 organic compounds (principally organochlorine pesticide residues). Three compounds were detected at the two sites: DDD was found at 0.2 microgram per kilogram, DDE was detected at 0.1 microgram per kilogram at both sites, and chlordane was detected at 1 microgram per kilogram. None of the 28 whole-fish and fillet samples analyzed for PCB and organochlorine pesticide residues contained any of the 23 organic compounds tested for at concentrations higher than the analytical reporting limit (less than 0.01 μ g/g wet weight). Concentrations of inorganic analytes were generally within established guidelines or expected concentrations for water, sediment, and biota. lirigation-return flows were found to be unrelated to adverse effects in biota.

New Mexico