Precision of four calcified structures for age estimation of Black Carp

Abstract

Objective Black Carp Mylopharyngodon piceus is an emerging invasive species in North America with an expanding population in the Mississippi River basin. Current aging methods use a suite of structures for age estimation, and a single structure is needed to minimize processing time, to maximize consistency of age and growth measurements, and to allow for back-calculation of individual fish length at age. Methods In total, 236 Black Carp were collected throughout the year from 2017 to 2019 through incidental captures by commercial fishers and biologists. In a subsample, 119 Black Carp from 429 to 1268 mm total length were compared for the precision of annuli counts by two experienced readers for the two anterior pectoral fin rays, the anterior dorsal fin ray, sectioned vertebrae, and lapilli otoliths based on percent exact agreement (PA), percent agreement within 1 year (PA ± 1), coefficient of variation (CV), and bubble plots. Consensus annuli counts were compared by PA and CV between structures. We applied annuli counts, as opposed to age, as fish were collected during all seasons and structures were examined independently by readers without knowledge of capture date. Result Vertebrae and dorsal rays had the highest PA between readers. Dorsal ray had the lowest CV and higher PA ± 1. Pectoral rays and lapilli otoliths had the lowest PA. Between-structure consensus annuli counts indicated that pectoral rays and the dorsal ray annuli counts were the most similar (PA >70% and CV <12) and lapilli otoliths were the most dissimilar (PA >35% and CV >24). Reader-reported confidence was highest for vertebrae, then in decreasing order, dorsal ray, pectoral rays, and lapilli otoliths. Lumens were present in all fin rays with only minor effects observed on annuli counts, aside from the anterior-most pectoral fin ray, in which lumens obscured the presence of one or more potential annuli in 61% of structures. Conclusion The dorsal ray had the highest agreement between readers, with agreement with ±1 year at 98%; the structure can be efficiently extracted and processed, and the structure's morphology and growth pattern are conducive to aging older fish. Vertebrae had high agreement between readers, but a systematic pattern of double bands in vertebrae increased the between-reader variation in older fish as the interannual growth pattern compressed the viewable area; this was identified by readers based on aging criteria and highlights the need for training novice readers. Validation of annual growth in the tested suite of structures is still needed.