Development and characterization of polymorphic microsatellitemarkers for the crested caracara, Caracara cheriway

Erin E. Vaughn; James F. Dwyer; Joan L. Morrison; Melanie Culver

doi:10.1007/s12686-015-0423-9

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Development and characterization of polymorphic microsatellitemarkers for the crested caracara, Caracara cheriway

Erin E. Vaughn·James F. Dwyer·Joan L. Morrison·Melanie Culver

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Abstract

We isolated novel microsatellites from the crested caracara ( Caracara cheriway ) with a shotgun pyrosequencing approach. We tested 80 loci for polymorphism among 20 individuals from the threatened Florida population. Fourteen loci were polymorphic. The mean number of alleles was 2.21 (range 2–3) and the mean observed heterozygosity was 0.41 (range 0.15–0.65). None of the 14 polymorphic loci exhibited significant linkage disequilibrium nor did they deviate significantly from Hardy–Weinberg expectations. We also report 16 monomorphic loci.

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Source-reported bounding extent: 24.546468° to 31.000682° latitude; -87.634938° to -80.031362° longitude. This indicates report coverage, not an exact sampling location. View area on OpenStreetMap.

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Erin E. Vaughn, James F. Dwyer, Joan L. Morrison, Melanie Culver. 2015-01-30. Development and characterization of polymorphic microsatellitemarkers for the crested caracara, Caracara cheriway. https://doi.org/10.1007/s12686-015-0423-9

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