Determination of Antimycin-A in water by liquid chromatographic/mass spectrometry: single-laboratory validation

Jeffry A. Bernardy; Terrance D. Hubert; Jacob M. Ogorek; Larry J. Schmidt

doi:10.5740/jaoacint.12-286

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Determination of Antimycin-A in water by liquid chromatographic/mass spectrometry: single-laboratory validation

Jeffry A. Bernardy·Terrance D. Hubert·Jacob M. Ogorek·Larry J. Schmidt

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Abstract

An LC/MS method was developed and validated for the quantitative determination and confirmation of antimycin-A (ANT-A) in water from lakes or streams. Three different water sample volumes (25, 50, and 250 mL) were evaluated. ANT-A was stabilized in the field by immediately extracting it from water into anhydrous acetone using SPE. The stabilized concentrated samples were then transported to a laboratory and analyzed by LC/MS using negative electrospray ionization. The method was determined to have adequate accuracy (78 to 113% recovery), precision (0.77 to 7.5% RSD with samples ≥500 ng/L and 4.8 to 17% RSD with samples ≤100 ng/L), linearity, and robustness over an LOQ range from 8 to 51 600 ng/L.

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Jeffry A. Bernardy, Terrance D. Hubert, Jacob M. Ogorek, Larry J. Schmidt. 2019-11-27. Determination of Antimycin-A in water by liquid chromatographic/mass spectrometry: single-laboratory validation. https://doi.org/10.5740/jaoacint.12-286

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