Legumes in prairie restoration: evidence for wide cross-nodulation and improved inoculant delivery

Elena Beyhaut; Diane L. Larson; Deborah L. Allan; Peter H. Graham

doi:10.1007/s11104-013-1999-z

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Legumes in prairie restoration: evidence for wide cross-nodulation and improved inoculant delivery

Elena Beyhaut·Diane L. Larson·Deborah L. Allan·Peter H. Graham

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Abstract

Background and aims Prairie restoration aims to create self-sustaining, resilient prairies that ameliorate biodiversity loss and soil deterioration associated with conversion of native grasslands to agriculture. Legumes are a key component of the nitrogen-limited prairie ecosystem. Evidence suggests that lack of suitable rhizobia may explain legume absence from restored prairies. This study explores effects of novel alternative inoculant delivery methods on: (a) prairie legume establishment, (b) soil biological properties, and (c) inoculant strain ability to nodulate the host over time. Methods Alternative inoculation methods for seven legume species were tested in a replicated field experiment. Legume establishment, microbial biomass carbon and nitrogen, and rhizobial inoculant strain recovery were measured over a 3-year period. Results Legume species richness in the second growing season was enhanced by a soil-applied granular clay inoculant, while seed-applied powdered peat inoculation was generally ineffective. When Dalea rhizobia were recovered 3-year after planting, only 2 % from the seed-applied inoculation treatment identified with the inoculant strains, whereas this amount ranged from 53 to 100 % in the other inoculation treatments. Some legumes established unexpectedly effective symbioses with strains not originally intended for them. Conclusions Results provide new insights on inoculation of native legumes, especially when a mix of seeds is involved and the restoration targets harsh environments.

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Source-reported bounding extent: 45.3254° to 45.426° latitude; -93.9168° to -93.8195° longitude. This indicates report coverage, not an exact sampling location. View area on OpenStreetMap.

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Elena Beyhaut, Diane L. Larson, Deborah L. Allan, Peter H. Graham. 2013-12-22. Legumes in prairie restoration: evidence for wide cross-nodulation and improved inoculant delivery. https://doi.org/10.1007/s11104-013-1999-z

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