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Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction

P. Nol·J.L. Williamson·Tonie E. Rocke·Thomas M. Yuill
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Abstract

We established a method of directly detecting Clostridium botulinum type C cells, while minimizing spore detection, in the intestinal contents of Mozambique tilapia ( Oreochromis mossambicus ). This technique involved extraction of predominantly cellular DNA from tilapia intestinal tracts and used a polymerase chain reaction assay to detect presence of type C 1 toxin gene. We consistently detected C. botulinum type C cells in tilapia gastrointestinal contents at a level of 7.5×10 4 cells per 0.25 g material or 1.9×10 3 cells. This technique is useful for determining prevalence of the potentially active organisms within a given population of fish and may be adapted to other types of C. botulinum and vertebrate populations as well.

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Journal of Wildlife DiseasesArticle
90° N90° S · 180° W ← longitude → 180° E
Source-reported bounding extent: 33.05068781203998° to 33.54425617159974° latitude; -116.08978271484375° to -115.57273864746094° longitude. This indicates report coverage, not an exact sampling location. View area on OpenStreetMap.

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BibTeXRIS

P. Nol, J.L. Williamson, Tonie E. Rocke, Thomas M. Yuill. 2004. Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction. https://doi.org/10.7589/0090-3558-40.4.749

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