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USGS · 1016367

Evidence of decline for Bufo boreas and Rana luteiventris in and around the northern Great Basin

W. Wente·M. J. Adams·Christopher A. Pearl
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Abstract

A method is described for the analysis of polychlorinated biphenyl (PCB) compounds in tissue samples. Cleanup by hexane-aceto-nitrile partitioning and Florisil column chromatography are performed on samples before oxidative treatment to convert DDE to DCBP. PCB components are then determined semi-quantitatively by TLC. No prior separation of PCB from chlorinated pesticides is required. The lower limit of sensitivity is 0.2 ?g.

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W. Wente, M. J. Adams, Christopher A. Pearl. 2005. Evidence of decline for Bufo boreas and Rana luteiventris in and around the northern Great Basin. https://pubs.usgs.gov/publication/1016367

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