Regulation of gill cytosolic corticosteroid receptors in juvenile Atlantic salmon: Interaction effects of growth hormone with prolactand triiodothyronine

J. M. Shrimpton; S. D. McCormick

doi:10.1006/gcen.1998.7172

Home/Archive/USGS

USGS · 1014754

Regulation of gill cytosolic corticosteroid receptors in juvenile Atlantic salmon: Interaction effects of growth hormone with prolactand triiodothyronine

J. M. Shrimpton·S. D. McCormick

View original source ↗Export metadata ↓

Abstract

The potential effects of growth hormone (GH), prolactin (Prl), and triiodothyronine (T 3 ) on gill Na + ,K + -ATPase activity and corticosteroid receptor (CR) concentration ( B max ) and dissociation constant ( K d ) were examined in juvenile Atlantic salmon ( Salmo salar ). Compared to controls, fish injected with GH (ovine, 5.0 μg g −1 ) had significantly greater gill Na + ,K + -ATPase activity after 7 and 14 days. Gill CR B max and K d were significantly elevated on day 7, but not day 14. T 3 also significantly increased CR B max . The effect of GH on CR B max was also additive with T 3 (5.0 μg g −1 ) treatment. There was a synergistic effect on CR B max when purified coho salmon GH (csGH, 0.1 μg g −1 ) was injected in combination with T 3 (1.6 μg g −1 ). Prl (ovine, 5.0 μg g −1 ; purified coho salmon, 0.1 μg g −1 ) did not significantly alter gill CR B max . Although Prl limited the increase in CR B max by GH, the effect was not signicant. T 3 and Prl did not have an effect on K d . GH significantly increased gill Na + ,K + -ATPase activity, T 3 administration did not have a significant effect, and Prl-treated fish had significantly lower gill Na + ,K + -ATPase activity. The results indicate that T 3 acts additively with GH, while Prl has no effect in regulating CR B max . An increase in cytosolic CR by GH and T 3 , but not Prl, may regulate gill responsiveness to cortisol and be an important mechanism in the endocrine control of physiological changes during the parr–smolt transformation.

Explore related subjects

General and Comparative Endocrinology Article

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

J. M. Shrimpton, S. D. McCormick. 1998. Regulation of gill cytosolic corticosteroid receptors in juvenile Atlantic salmon: Interaction effects of growth hormone with prolactand triiodothyronine. https://doi.org/10.1006/gcen.1998.7172

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related USGS reports

USGS2026

Characterizing the annual cycle of steroid hormones in males from an invasive vertebrate (Python bivittatus) of management concern

South Florida's subtropical climate has facilitated the establishment of numerous invasive species, including the Burmese python ( Python bivittatus ). Despite decades of efforts to manage the invasive python population across South Florida, we lack a comprehensive understanding of their reproductive physiology, limiting the development of targeted control strategies. Here, we characterized seasonal patterns of testosterone (T) and corticosterone (CORT) in free-ranging adult male pythons and evaluated environmental correlates of hormone variation. Results indicate that T peaks before the onset of the breeding season and declines through the season to baseline levels in the non-breeding season. CORT did not show a clear seasonal trend but showed greater variability during the breeding season. These findings reveal that photoperiod and ambient temperature are key environmental correlates of male hormone cycles, contributing to our understanding of the endocrine phenology of a tropical ectotherm in a novel environment.

Florida↗

USGS2023

Low estradiol production of non-laying whooping cranes (Grus americana) is associated with the failure of small follicles to enter follicular hierarchy

For endangered species managed ex situ , production of offspring is a key factor to ensure healthy and self-sustaining populations. However, current breeding goals for the whooping crane ( Grus americana ) are impeded by poor reproduction. Our study sought to better understand mechanisms regulating ovarian function in ex situ managed whooping cranes and the regulatory function of the hypothalamic-pituitary-gonadal (HPG) axis in relation to follicle formation and egg laying. To characterize hormonal regulation of follicular development and ovulation, we collected weekly blood samples from six female whooping cranes during two breeding seasons, for a total of 11 reproductive cycles. The plasma samples were assessed for follicle stimulating hormone, luteinizing hormone, estradiol, and progesterone and the yolk precursors vitellogenin and very low-density lipoprotein. Ultrasonographic examination of the ovary was conducted at the time of blood collection. Preovulatory follicles (>12 mm) were present in laying cycles (n = 6) but absent in non-laying cycles (n = 5). The patterns of plasma hormone and yolk precursor concentrations corresponded to the stage of follicle development. Specifically, gonadotropin and yolk precursors concentrations increased as follicles transitioned from the non-yolky to yolky stage but did not increase further as the follicle advanced to preovulatory and ovulatory stages. Estrogen and progesterone concentrations increased as follicle size increased and reached peak concentrations (P < 0.05) when follicles developed to ovulatory and preovulatory stages, respectively. While overall mean circulating gonadotropin, progesterone, and yolk precursor concentrations did not differ for laying versus non-laying cycles, mean plasma estradiol in laying cycles was significantly higher than that in non-laying cycles. In summary, the findings suggested that disruption of mechanisms regulating follicle recruitment is likely responsible for the oviposition failure of the captive female whooping crane.

General and Comparative Endocrinology↗

USGS2022

Effects of salinity and a glucocorticoid antagonist, RU486, on waterborne aldosterone and corticosterone of northern leopard frog larvae

Increased salinity is an emerging contaminant of concern for aquatic taxa. For amphibians exposed to salinity, there is scarce information about the physiological effects and changes in osmoregulatory hormones such as corticosterone (CORT) and aldosterone (ALDO). Recent studies have quantified effects of salinity on CORT physiology of amphibians based on waterborne hormone collection methods, but much less is known about ALDO in iono- and osmoregulation of amphibians. We re-assayed waterborne hormone samples from a previous study to investigate effects of salinity (sodium chloride, NaCl) and a glucocorticoid receptor antagonist (RU486) on ALDO of northern leopard frog ( Rana pipiens) larvae. We also investigated relationships between ALDO and CORT. Waterborne ALDO marginally decreased with increasing salinity and was, unexpectedly, positively correlated with baseline and stress-induced waterborne CORT. Importantly, ALDO increased when larvae were exposed to RU486, suggesting that RU486 may also suppress mineralocorticoid receptors or that negative feedback of ALDO is mediated through glucocorticoid receptors. Alternatively, CORT increases with RU486 treatment and might be a substrate for ALDO synthesis, which could account for increases in ALDO with RU486 treatment and the correlation between CORT and ALDO. ALDO was negatively correlated with percent water, such that larvae secreting more ALDO retained less water. Although sample sizes were limited and further validation and studies are warranted, our findings expand our understanding of adrenal steroid responses to salinization in amphibians and proposes new hypotheses regarding the co-regulation of ALDO and CORT.

General and Comparative Endocrinology↗