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Research about Wisconsin

Source-linked reports with geographic coverage including Wisconsin.

At least 73 records · Page 4Linked to original sources

Occurrence of mixed organic and inorganic chemicals in groundwater and tapwater, town of Campbell, Wisconsin, 2021–22

In response to previous reports of per- and polyfluoroalkyl substances (PFAS) contamination in French Island’s (located in the Mississippi River within the town of Campbell, Wisconsin) primary source of drinking water, 11 locations were sampled by the U.S. Geological Survey (USGS) in October 2021 to assess the potential presence of contaminant mixtures, including PFAS, in tapwater. Three locations were assessed seven times each over the course of three days. These samples were chosen to evaluate the water quality of the deeper Mount Simon bedrock aquifer and the water quality of the shallower sand and gravel (alluvial) aquifer at two locations. The other eight sample locations were spatially distributed within Campbell and were sampled once each. For each of these 11 sites, tapwater samples were analyzed for disinfection byproducts (DBP), pesticides, PFAS, pharmaceuticals, semi-volatile organic compounds (SVOC), volatile organic compounds (VOC), cations, anions, trace elements, alkalinity, microbial indicators, as well as measurements of water temperature, specific conductance, and pH. Of the 506 organic compounds analyzed in each water-quality sample, 74 (14 percent) were detected at least one time in any of the samples collected. Of the 14 percent, detected analytes included 27 pesticides (5 percent), 14 PFAS (3 percent), 6 pharmaceuticals (1 percent), 7 SVOC (1 percent), and 20 VOC (4 percent). No DBP were detected. The total number of organic compounds detected per sample ranged from 0–20 (median of 10), with the sum of concentrations ranging from not detected (nd)–2.53 micrograms per liter (μg/L; median of 0.333 μg/L). Of the inorganic constituents measured, eight were not detected above their reporting limit in any of the samples. The inorganic constituents that were not detected were antimony, arsenic, beryllium, cadmium, cobalt, molybdenum, selenium, and vanadium. Along with the 11 sites sampled throughout Campbell, Wisconsin, beginning in October 2021, four more wells were sampled on the Upper Midwest Environmental Sciences Center (UMESC) campus for PFAS. Three of these sites withdraw water from the shallow alluvial aquifer (the same source water for tapwater site 002) and one from the Mount Simon aquifer (the same source of water for tapwater site 001). This sampling is ongoing with results from samples through December 2022 summarized in this report. Of the 33 PFAS analyzed in samples from the four UMESC locations, 15 individual PFAS were detected at least one time in any of the samples analyzed with the sum of PFAS concentrations ranging from nd–1.49 μg/L (median of 0.309 μg/L).

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Scale-dependence in elk habitat selection for a reintroduced population in Wisconsin, USA

Habitat selection is a critical aspect of a species' ecology, requiring complex decision-making that is both hierarchical and scale-dependent, since factors that influence selection may be nested or unequal across scales. Elk ( Cervus canadensis ) ranged widely across diverse ecoregions in North America prior to European settlement and subsequent eastern extirpation. Most habitat selection studies have occurred within their contemporary western range, even after eastern reintroductions began. As habitat selection can vary by geographic location, available cover, season, and diel period, it is important to understand how a non-migratory, reintroduced population in northern Wisconsin, USA, is limited by the lack of variation in topography, elevation, and vegetation. We tested scale-dependent habitat selection on 79 adult elk from 2017 to 2020 using resource selection functions across temporal (i.e., seasonal) and spatial scales (i.e., landscape and home range). We found that selection varied both spatially and temporally, and elk selected areas with the greatest potential to influence fitness at larger scales, meaning elk selected areas closer to escape cover and further from “risky” features (e.g., annual wolf territory centers, county roads, and highways). We found stronger avoidance of annual wolf territory centers during spring, suggesting elk were selecting safer habitats during calving season. Elk selected habitats with less canopy cover across both spatial scales and all seasons, suggesting that elk selected areas with better access to forage as early seral stage stands have greater forage biomass than closed-canopy forests and direct solar radiation to provide warmth in the cooler seasons. This study provides insight into the complexity of hierarchical decision-making, such as how risky habitat features and land cover type influence habitat selection differently across seasons and spatial scales, influencing the decision-making of elk. Scale-dependent behavior is crucial to understand within specific geographic regions, as these decisions scale up to influence population dynamics.

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Design and calibration of a nitrate decision support tool for groundwater wells in Wisconsin, USA

This paper describes development of a nitrate decision support tool for groundwater wells (GW-NDST) that combines nitrate leaching and groundwater lag-times to compute well concentrations. The GW-NDST uses output from support models that simulate leached nitrate, groundwater age distributions, and nitrate reduction rates. The support models are linked through convolution to simulate nitrate transport to wells. Spatially distributed parameters were adjusted through calibration to 34,255 nitrate sample targets. Prediction uncertainty is illustrated via Monte Carlo realizations informed during calibration. Over 78% of target concentrations were within the simulated range of results from 450 realizations. An example forecasting scenario illustrates that a range of feasible outcomes exist and should be considered when interpreting forecasts for decision making. Uncertainty in forecasting is unavoidable; the intent of characterizing uncertainty in the GW-NDST is to facilitate decision making by increasing insight into the response of nitrate contamination to physical and chemical processes.

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Assessing the relationship between cyanobacteria blooms and respiratory-related hospital visits: Green Bay, Wisconsin 2017–2019

Potential acute and chronic human health effects associated with exposure to cyanobacteria and cyanotoxins, including respiratory symptoms, are an understudied public health concern. We examined the relationship between estimated cyanobacteria biomass and the frequency of respiratory-related hospital visits for residents living near Green Bay, Lake Michigan, Wisconsin during 2017–2019. Remote sensing data from the Cyanobacteria Assessment Network was used to approximate cyanobacteria exposure through creation of a metric for cyanobacteria chlorophyll-a (Chl BS ). We obtained counts of hospital visits for asthma, wheezing, and allergic rhinitis from the Wisconsin Hospital Association for ZIP codes within a 3-mile radius of Green Bay. We analyzed weekly counts of hospital visits versus cyanobacteria, which was modelled as a continuous measure (Chl BS ) or categorized according to World Health Organization's (WHO) alert levels using Poisson generalized linear models. Our data included 2743 individual hospital visits and 114 weeks of satellite derived cyanobacteria biomass indicator data. Peak values of Chl BS were observed between the months of June and October. Using the WHO alert levels, 60% of weeks were categorized as no risk, 19% as Vigilance Level, 15% as Alert Level 1, and 6% as Alert Level 2. In Poisson regression models adjusted for temperature, dewpoint, season, and year, there was no association between Chl BS and hospital visits (rate ratio [RR] [95% Confidence Interval (CI)] = 0.98 [0.77, 1.24]). There was also no consistent association between WHO alert level and hospital visits when adjusting for covariates (Vigilance Level: RR [95% CI] 0.88 [0.74, 1.05], Alert Level 1: 0.82 [0.67, 0.99], Alert Level 2: 0.98 [0.77, 1.24], compared to the reference no risk category). Our methodology and model provide a template for future studies that assess the association between cyanobacterial blooms and respiratory health.

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Role of tributary cyanobacterial and nutrient transport and sediment processes on cyanobacterial bloom initiation in Lake Superior nearshore

Watershed fluxes of suspended sediment (SS), nutrients, in particular phosphorus (P), and cyanobacteria may play a role in driving cyanobacterial blooms along the southwestern shore of oligotrophic Lake Superior. To understand how tributary loads contribute to nearshore blooms, we sampled two southwestern shore tributaries, Bois Brule and Siskiwit Rivers. We collected water-quality samples to compute nutrient and sediment loads and to assess cyanobacteria community composition from the tributaries to the nearshore. We collected suspended and streambed sediment to assess the capacity for sediment to store and transport bioavailable P and to assess cyanobacteria community composition. Storm flows drove export of SS, total P, and total nitrogen, with the majority of total P being particulate P. Equilibrium P concentrations revealed that SS sorbed P as it is moved through the stream network across sites and seasons and was a potential source of P to the nearshore. However, streambed sediment in the Bois Brule and Siskiwit River watersheds were P sinks during summer, which potentially delayed transport of dissolved P to the lake. The cyanobacteria community varied spatially and temporally relating to multiple environmental variables including nutrients (P, N, and C) and specific conductivity. Cyanobacteria capable of producing cyanotoxins were present in tributaries and found across multiple environmental compartments indicating a potential for fluvial flow to the nearshore. This study demonstrated that streamflow is a primary driver of total nutrient and sediment loading in both watersheds, which indicates the potential for algal loading to the nearshore via suspended sediment or water.

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Influences of meteorological conditions, runoff, and bathymetry on summer thermal regime of a Great Lakes estuary

To better understand the primary drivers of the thermal regime in a Great Lakes estuary, and their implications for local biota, water temperature variations in the Milwaukee Estuary of Lake Michigan were studied between July and October of 2019 using a network of 25 sensors at 18 locations. Like Lake Michigan, the estuary was thermally stratified July to October, and historically dredged channels with water depths greater than 6 m allowed for the free movement of cold lake water into the estuary. However, temperatures in the estuary fluctuated rapidly both spatially and temporally, reflecting lake temperature fluctuations driven by changing meteorological conditions. Lake-driven upwelling and downwelling events influenced water temperature more than tributary contributions. Periodic upwelling and downwelling events caused temperature changes by up to 15 °C in less than 24 h. Nearshore upwelling events occasionally allowed cold, hypolimnetic lake water to reach tributary portions of the estuary beyond dredged areas, while downwelling events disrupted thermal stratification and caused the deep, dredged portions of the estuary to exceed 20 °C. Thermal impacts from these events were especially noticeable in transition zones between dredged and not dredged channels less than 2 m deep. The warming effects from downwelling persisted longer inside the estuary – up to two weeks – than cooling effects from upwelling, which typically lasted a few days. The predominantly lake-driven, rapid summer water temperature fluctuations observed in the Milwaukee Estuary serve as an important consideration in habitat restoration activities happening in Great Lakes estuaries.

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A multi-marker assessment of sewage contamination in streams using human-associated indicator bacteria, human-specific viruses, and pharmaceuticals

Human sewage contaminates waterways, delivering excess nutrients, pathogens, chemicals, and other toxic contaminants. Contaminants and various sewage indicators are measured to monitor and assess water quality, but these analytes vary in their representation of sewage contamination and the inferences about water quality they support. We measured the occurrence and concentration of multiple microbiological ( n = 21) and chemical ( n = 106) markers at two urban stream locations in Milwaukee, Wisconsin, USA over two years. Five-day composite water samples ( n = 98) were collected biweekly, and sewage influent samples ( n = 25) were collected monthly at a Milwaukee, WI water reclamation facility. We found the vast majority of markers were not sensitive enough to detect sewage contamination. To compare analytes for monitoring applications, five consistently detected human sewage indicators were used to evaluate temporal patterns of sewage contamination, including microbiological (pepper mild mottle virus, human Bacteroides , human Lachnospiraceae ) and chemical (acetaminophen, metformin) markers. The proportion of human sewage in each stream was estimated using the mean influent concentration from the water reclamation facility and the mean concentration of all stream samples for each sewage indicator marker. Estimates of instream sewage pollution varied by marker, differing by up to two orders of magnitude, but four of the five sewage markers characterized Underwood Creek (mean proportions of human sewage ranged 0.0025 % - 0.075 %) as less polluted than Menomonee River (proportions ranged 0.013 % - 0.14 %) by an order of magnitude more. Chemical markers correlated with each other and yielded higher estimates of sewage pollution than microbial markers, which exhibited greater temporal variability. Transport, attenuation, and degradation processes can influence chemical and microbial markers differently and cause variation in human sewage estimates. Given the range of potential human and ecological health effects of human sewage contamination, robust characterization of sewage contamination that uses multiple lines of evidence supports monitoring and research applications.

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Diminishing productivity and hyperstable harvest in northern Wisconsin walleye fisheries

Managing fisheries in a changing socio-ecological environment may require holistic approaches for identifying and adapting to novel ecosystem dynamics. Using 32 years of Ceded Territory of Wisconsin (CTWI) walleye (Sander vitreus) data, we estimated production (P), biomass (B), biomass turnover (P/B), yield (Y), and yield over production (Y/P) and tested for hyperstability in walleye yield. Most CTWI walleye populations showed low P, and B, and Y/P < 1. Yet, production overharvest (Y/P > 1) was prevalent among Wisconsin walleye recruitment-based management approaches (natural recruitment [NR], sustained only by stocking, combination). Production, B, and P/B have declined in NR populations, while Y and Y/P have remained constant. Walleye Y was hyperstable along a production gradient among all management approaches and fishery types (i.e., angling only, angling/tribal harvest combined). Diminishing productivity and hyperstable yield may be jointly contributing to observed walleye declines. We classified lakes into management groups of low, moderate, or high vulnerability to harvest based on Y/P and P/B dynamics and recommend that exploitation may need to decline to maintain or increase the adaptive capacity of CTWI walleye.

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Effective field sampling of rectoanal mucosa-associated lymphoid tissue for antemortem chronic wasting disease testing in white-tailed deer

Chronic wasting disease (CWD) is a fatal prion disease of cervids that has spread across much of North America. Although gold standard CWD diagnostics involve postmortem testing of medial retropharyngeal lymph nodes or obex (brain stem), a key tissue sample for antemortem testing is rectoanal mucosa–associated lymphoid tissue (RAMALT). However, collection of an adequate sample (i.e., enough lymphoid follicles) may be affected by factors such as deer age, repeated sampling, skill of the sampler, and adverse conditions during collection. Here, we document the protocol used to train personnel for RAMALT collection in a large study of free-ranging white-tailed deer ( Odocoileus virginianus ) in Wisconsin, USA, and determine factors that contributed to the occurrence of inadequate RAMALT samples. Our training protocol included hands-on experience with postmortem tissues, as well as a mentored collection process in the field. Collection of RAMALT under field conditions was highly successful, with 763/806 (94.7%) samples deemed adequate for subsequent testing. Although inadequate samples were rare, they were more likely to occur with older deer and when samples were collected at dusk (i.e., limited ambient lighting). We conclude that RAMALT collection can be highly successful under adverse field conditions, including with technicians with limited prior veterinary experience, and we provide details of our training program to facilitate repeatability in other antemortem CWD testing efforts.

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Changes in phosphorus and suspended solids loading in the Fox River, northeastern Wisconsin, 1989–2021

The entire Lower Fox River and inner bay of Green Bay, in northeastern Wisconsin, have been listed as impaired by the Wisconsin Department of Natural Resources (WDNR) for low dissolved oxygen and degraded habitat, with total phosphorus (TP) and total suspended solids (TSS) concentrations listed as the likely causes of these impairments. To restore the Fox River and Green Bay, total maximum daily loads (TMDLs) were developed for TP and TSS, and actions were taken throughout the Fox River Basin to improve water quality. In this study, we estimated concentrations and loads of TP, dissolved phosphorus (DP), and TSS at the Lake Winnebago outlet, De Pere, and the mouth of the Fox River from water year (WY) 1989 to WY 2021; described changes in concentrations and loads through time during this period; and compared the concentrations and loads for the most recent 5-year period (WYs 2017–21) with the WDNR criteria for TP impairment and the TMDL loading goals. TP, DP, TSS, and total suspended sediment concentration data were obtained from NEW Water (the brand of the Green Bay Metropolitan Sewerage District), the WDNR, and the U.S. Geological Survey and combined into one dataset. All the TSS and total suspended sediment data were used together with no adjustment factor and are referred to as simply “TSS.” During WYs 1989–2021, mean annual TP concentrations increased from 0.089 milligram per liter (mg/L) at the Lake Winnebago outlet to 0.128 mg/L at the mouth of the Fox River, and concentrations decreased at all three sites from WY 1989 to WY 2021. The most recent (WYs 2017–21) median May–October TP concentrations were just less than the 0.1-mg/L WDNR criterion for TP impairment at the two upstream sites (Lake Winnebago outlet and De Pere) but were slightly greater than the criterion for impairment at the mouth of the Fox River. Mean annual DP concentrations increased from 0.024 mg/L at the Lake Winnebago outlet to 0.036 mg/L at the mouth of the Fox River. DP concentrations increased from WY 1989 to WY 2021 at the Lake Winnebago outlet but not at the other sites. Mean annual TSS concentrations increased from 13.5 mg/L at the Lake Winnebago outlet to 23.9 mg/L at the mouth of the Fox River and have decreased at all three sites from WY 1989 to WY 2021. The recent median May–October TSS concentrations were less than the 20-mg/L WDNR criterion for impairment at all three sites. Streamflow and TP, DP, and TSS loads increased from the Lake Winnebago outlet to the mouth of the Fox River (TP loads increased from 360,000 to 557,000 kilograms per year [kg/yr], DP loads increased from 114,000 to 162,000 kg/yr, and TSS loads increased from 60,400 metric tons per year [t/yr] to 122,600 t/yr). At the Lake Winnebago outlet, DP concentrations and TP and DP loads increased from WY 1989 to WY 2021 because of an increase in DP concentrations in Lake Winnebago resulting from the lake becoming nitrogen limited as a result of biological processes not consuming the DP in the lake and an increase in streamflow leaving the lake. Although TP and TSS concentrations decreased at De Pere and the mouth of the Fox River, there was little change in the loading because of an increase in flow. Flow-normalized TP and TSS loads at De Pere and the mouth of the Fox River decreased possibly because of implementation of agricultural conservation management practices, reductions in point-source discharges in its drainage basin, and deposition of sediment and phosphorus in recently dredged areas of the Lower Fox River. Additional studies are needed to determine the relative importance of each of these actions and whether the decrease in concentrations and flow-normalized loads will continue to be observed in the Fox River.

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Cisco population characteristics in Wisconsin lakes in relation to lake- and landscape-level factors

Objective Declines in Cisco Coregonus artedi populations in some inland lakes have prompted assessments of Cisco occurrence and extirpation risk in relation to various stressors to identify refuge lakes and factors that promote Cisco persistence. However, most previous assessments have focused on presence–absence of Cisco rather than examining how population characteristics, such as relative abundance or growth, might change in relation to lake- and landscape-level environmental factors. Consequently, our specific objectives were to identify important environmental factors explaining variation in Cisco relative abundance and growth and to determine whether population metrics describing size and age distributions were related to relative abundance in Wisconsin inland lakes. Methods Cisco were collected from 48 inland Wisconsin lakes during 2011–2015 using vertical monofilament gill nets and population-specific relative abundance estimates (catch per unit effort [CPUE]) were quantified as the number of individuals per gill-net night. Sagittal otoliths were removed from a subsample of Cisco for age estimation and growth was indexed as mean total length (TL; mm) at age 2. Length and age data were used to develop a suite of metrics describing size and age distributions of each population. Random forest models were used to evaluate relationships between 10 biologically relevant predictor variables representing variation in physical, climatic, catchment, and limnological characteristics and Cisco CPUE and growth. Pearson correlations were used to determine whether population characteristics were related to CPUE. Result Cisco populations exhibited large variation in relative abundance, growth, and size and age distributions. Best-fit random forest models explained approximately 25% of the variation in Cisco CPUE and 46% of the variation in growth. Growing degree-days and variables associated with availability, quality, and quantity of suitable oxythermal conditions were identified as important predictors of both Cisco CPUE and growth; CPUE was also identified as an important predictor of growth. Mean TL and mean TL at age 2 were negatively related to Cisco CPUE, whereas mean age, number of age-classes present, and maximum observed age were positively related to CPUE. Conclusion Our results suggest that maintenance of suitable oxythermal habitat conditions may be critical to conserving abundant Cisco populations. Our assessment also provides insights on how Cisco populations may respond to environmental and anthropogenic stressors, which could aid ongoing and future conservation and management efforts in Wisconsin and elsewhere.

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Airport deicers: An unrecognized source of phosphorus loading in receiving waters

Airport ice control products contributed to total phosphorus (TP) loadings in a study of surface water runoff at a medium-sized airport from 2015 to 2021. Eleven airport ice control products had TP concentrations from 1–807 mg L –1 in liquid formulas, while solid pavement deicer had a TP concentration of 805 mg kg –1 . Product application data, formula TP concentrations, and surface water sampling results were used to estimate TP concentration and loading contributions from these ice control products to receiving streams. Airport ice control products were found to contribute to TP in 84% of the water samples collected at downstream sites during deicing events, and TP concentrations at those sites exceeded aquatic life benchmarks in 70% of samples collected during deicing. A receiving stream 6 km downstream had TP attributed to airport ice control sources in 78% of the samples. TP loadings at an upstream site and the receiving stream site were greatest during the largest runoff events as is typical in urban runoff, but this pattern was not always followed at airport outfall sites due to the influence of TP in deicer products. Products analyzed in this study are used at airports across the United States and abroad, and findings suggest that airport deicers could represent a previously unrecognized source of phosphorus to adjacent waterways.

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Identifying sources of antibiotic resistance genes in the environment using the microbial Find, Inform, and Test framework

Introduction: Antimicrobial resistance (AMR) is an increasing public health concern for humans, animals, and the environment. However, the contributions of spatially distributed sources of AMR in the environment are not well defined. Methods: To identify the sources of environmental AMR, the novel microbial Find, Inform, and Test (FIT) model was applied to a panel of five antibiotic resistance-associated genes (ARGs), namely, erm(B), tet(W), qnrA, sul1, and intI1, quantified from riverbed sediment and surface water from a mixed-use region. Results: A one standard deviation increase in the modeled contributions of elevated AMR from bovine sources or land-applied waste sources [land application of biosolids, sludge, and industrial wastewater (i.e., food processing) and domestic (i.e., municipal and septage)] was associated with 34–80% and 33–77% increases in the relative abundances of the ARGs in riverbed sediment and surface water, respectively. Sources influenced environmental AMR at overland distances of up to 13 km. Discussion: Our study corroborates previous evidence of offsite migration of microbial pollution from bovine sources and newly suggests offsite migration from land-applied waste. With FIT, we estimated the distance-based influence range overland and downstream around sources to model the impact these sources may have on AMR at unsampled sites. This modeling supports targeted monitoring of AMR from sources for future exposure and risk mitigation efforts.

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Fall contributions of phosphorus and nitrogen in stormwater runoff through weekly street cleaning

This report describes a study that characterized reductions in total and dissolved forms of phosphorus and nitrogen in stormwater runoff through implementation of a municipal leaf collection and street cleaning program in two medium-density residential catchments in Madison, Wisconsin. One catchment was established as a control in which no effort was made to remove leaf litter and other organic detritus from streets. The second catchment served as the test catchment in which leaf litter was removed through a combination of biweekly leaf collection and weekly mechanical-broom street cleaning. Loads of total and dissolved phosphorus in the test catchment were reduced by 46 and 51 percent (probability less than 0.20), respectively, and total and dissolved nitrogen by 42 and 52 percent (probability less than 0.20), respectively, with an active leaf collection and street cleaning program compared to no program in the control catchment. Results from this study support an ongoing effort to characterize how various combinations of municipal leaf collection and street cleaning can affect nutrient loads in urban runoff.

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Response in the water quality of Delavan Lake, Wisconsin, to changes in phosphorus loading—Setting new goals for loading from its drainage basin

During 1989–92, an extensive rehabilitation project was completed in and around Delavan Lake, Wisconsin, to improve the lake’s water quality. However, in 2016, the lake was listed by the Wisconsin Department of Natural Resources as impaired for excessive algal growth (high chlorophyll a concentrations), and high phosphorus input was listed as its likely cause. In addition, the recent (2017–21) mean summer water clarity (as measured with a Secchi disk) was shallower than the goal set by the community (3.0 meters). Based primarily on flow and water-quality data collected in Jackson Creek, which is the main tributary of the lake, the mean annual phosphorus loading to the lake during water years (WYs) 2017–21 was 6,570 kilograms per year (kg/yr), and 306 kg/yr came from uncontrollable sources (atmospheric deposition and groundwater). Phosphorus loading during these years was about 48 percent higher than the long-term mean loading from WY 1984 to WY 2021. Based on results from Canfield-Bachmann phosphorus models, Carlson trophic state index relations, and the Jones and Bachmann chlorophyll a relation, external phosphorus loading would need to be decreased from 6,570 to 5,270 kg/yr (a 21-percent reduction in the potentially controllable external phosphorus load from the base period of WYs 2017–21) for chlorophyll a concentrations greater than 20 micrograms per liter to be detected no more than 5.0 percent of the time (the Wisconsin Department of Natural Resources criterion for chlorophyll a impairment for the lake). Based on Carlson trophic state index relations, external loading would need to be decreased from 6,570 to 4,380 kg/yr (a 35-percent reduction in the potentially controllable external phosphorus load) for summer mean Secchi depths to increase to 3.0 meters. Therefore, for Delavan Lake to reach the water-quality criteria for impairment and the goals for all three water-quality constituents, a 35-percent reduction in the potentially controllable phosphorus load is needed, which equates to a reduction in total phosphorus loading from 6,570 to 4,380 kg/yr. A 35-percent reduction in phosphorus loading to improve the water quality of Delavan Lake is less than the 49-percent reduction in phosphorus loading required for the area near Delavan Lake to improve the water quality of the Rock River and its tributaries indicated in the Rock River total maximum daily load.

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Movement and behavioral states of common carp (Cyprinus carpio) in response to a behavioral deterrent in a navigational lock

Freshwater ecosystems are some of the most affected by biological invasions due, in part, to the introduction of invasive carp worldwide. Where carp have become established, management programs often seek to limit further range expansion into new areas by reducing their movement through interconnected rivers and waterways. Lock and dams are important locations for non-physical deterrents, such as carbon dioxide (CO 2 ), to reduce unwanted fish passage without disrupting human use. The purpose of this study was to evaluate the behavioral responses of common carp ( Cyprinus carpio ) to non-physical deterrents within a navigation structure on the Fox River, Wisconsin. Acoustic telemetry combined with hidden Markov models (HMMs) was used to analyze variation in carp responses to treatments. Outcomes may inform CO 2 effectiveness at preventing invasive carp movement through movement pinch-points.

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Modeled predictions of human-associated and fecal-indicator bacteria concentrations and loadings in the Menomonee River, Wisconsin using in-situ optical sensors

Human sewage contamination of waterways is a major issue in the United States and throughout the world. Models were developed for estimation of two human-associated fecal-indicator and three general fecal-indicator bacteria (HIB and FIB) using in situ optical field-sensor data for estimating concentrations and loads of HIB and FIB and the extent of sewage contamination in the Menomonee River in Milwaukee, Wisconsin. Three commercially available optical sensor platforms were installed into an unfiltered custom-designed flow-through system along with a refrigerated automatic sampler at the Menomonee River sampling location. Ten-minute optical sensor measurements were made from November 2017 to December 2018 along with the collection of 153 flow-weighted discrete water samples (samples) for HIB, FIB, dissolved organic carbon (DOC), and optical properties of water. Of those 153 samples, 119 samples were from event-runoff periods, and 34 were collected during low-flow periods. Of the 119 event-runoff samples, 43 samples were from event-runoff combined sewer overflow (CSO) influenced periods (event-CSO periods). Models included optical sensor measurements as explanatory variables with a seasonal variable as an interaction term. In some cases, separate models for event-CSO periods and non CSO-periods generally improved model performance, as compared to using all the data combined for estimates of FIB and HIB. Therefore, the CSO and non-CSO models were used in final estimations for CSO and non-CSO time periods, respectively. Estimated continuous concentrations for all bacteria markers varied over six orders of magnitude during the study period. The greatest concentrations, loads, and proportion of sewage contamination occurred during event-runoff and event-CSO periods. Comparison to water quality standards and microbial risk assessment benchmarks indicated that estimated bacteria levels exceeded recreational water quality criteria between 34 and 96% of the entire monitoring period, highlighting the benefits of high-frequency monitoring compared to traditional grab sample collection. The application of optical sensors for estimation of HIB and FIB markers provided a thorough assessment of bacterial presence and human health risk in the Menomonee River.

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Evaluating pharmaceuticals and other organic contaminants in the Lac du Flambeau Chain of Lakes using risk-based screening techniques

In an investigation of pharmaceutical contamination in the Lac du Flambeau Chain of Lakes (hereafter referred to as “the Chain”), few contaminants were detected; only eight pharmaceuticals and one pesticide were identified among the 110 pharmaceuticals and other organic contaminants monitored in surface water samples. This study, conducted in cooperation with the Lac du Flambeau Tribe’s Water Resource Program, investigated these organic contaminants and potential biological effects in channels connecting lakes throughout the Chain, including the Moss Lake Outlet site, adjacent to the wastewater treatment plant lagoon. Of the 6 sites monitored and 24 samples analyzed, sample concentrations and contaminant detection frequencies were greatest at the Moss Lake Outlet site; however, the concentrations and detection frequencies of this study were comparable to other pharmaceutical investigations in basins with similar characteristics. Because established water-quality benchmarks do not exist for the pharmaceuticals detected in this study, alternative screening-level water-quality benchmarks, developed using two U.S. Environmental Protection Agency toxicological resources (ToxCast database and ECOTOX knowledgebase), were used to estimate potential biological effects associated with the observed contaminant concentrations. Two contaminants (caffeine and thiabendazole) exceeded the prioritization threshold according to ToxCast alternative benchmarks, and four contaminants (acetaminophen, atrazine, caffeine, and carbamazepine) exceeded the prioritization threshold according to ECOTOX alternative benchmarks. Atrazine, an herbicide, was the most frequently detected contaminant (79% of samples), and it exhibited the strongest potential for biological effects due to its high estimated potency. Insufficient toxicological information within ToxCast and ECOTOX for gabapentin and methocarbamol (which had the two greatest concentrations in this study) precluded alternative benchmark development. This data gap presents unknown potential environmental impacts. Future research examining the biological effects elicited by these two contaminants as well as the others detected in this study would further elucidate the ecological relevance of the water chemistry results generated though this investigation.

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