Geology topics

Research about United States

Source-linked reports with geographic coverage including United States.

811 records · Page 6Linked to original sources

USGS2026

Bathymetric maps, surface areas, and storage capacities of Council Grove Lake and Marion Reservoir, Kansas, and Pine Creek Lake, Oklahoma, 2024

The U.S. Geological Survey, in cooperation with the U.S. Army Corps of Engineers, completed high-resolution multibeam bathymetric surveys to compute new elevation-area-capacity tables for Council Grove Lake and Marion Reservoir, Kansas, and Pine Creek Lake, Oklahoma. Elevation-area-capacity tables identify the relation between the water-surface elevation, surface area, and storage capacity of the lake. The surface areas and storage capacities of each lake were computed from bathymetric surfaces combining multibeam echo sounder data collected in 2024 and light detection and ranging point-cloud data collected in 2016 and 2018.

Kansas, Oklahoma↗

USGS2026

A process-based model for forecasting wave runup along the coast of Georgia

Wave runup is an important nearshore process that impacts total water level, sediment transport, and coastal design. Current methods for forecasting wave runup implement an empirical model that considers offshore wave height, wave period, and generalized beach slope. In this study, the authors generated wave runup forecasts from offshore wave conditions and a system of polynomial equations derived from numerical simulations at three different still water datums for each beach profile. They developed a process-based methodology that incorporated site-specific cross-shore topobathy into the phase-resolving numerical model. A comparison between the system of equations, deterministic hydrodynamic simulations, and observed high-water marks was made using Hurricanes Matthew (2016) and Irma (2017) for 12 cases, and it showed that the polynomials were capable of being consistent with the results from full simulation runs, while not requiring hours of runtime when a forecast was needed—the differences between the polynomial and the observed high water marks ranged from 3 to 32 cm for the Irma hindcast and 9–70 cm for Matthew. Then, using forcings from Hurricanes Ian and Nicole (2022), the model predicted the occurrence of dune collision, overwash, and inundation for the coast of Georgia and suggested that wave runup was impacted by the still water level and local topobathy.

Georgia↗

USGS2026

Riverscape heterogeneity shapes population diversity for a migratory fish

Habitat patch dynamics can scale up to influence population demography and diversity with implications for resilience to environmental stochasticity. But how the spatial arrangement and size of habitat patches interact with other components of habitat heterogeneity to shape population diversity at larger spatial scales is not well understood. For riverine fishes, there is increasing evidence that tributary streams provide critical demographic support to main stem rivers. However, the extent to which main stem rivers rely on demographic contributions from tributaries, and the factors underlying this dependence, have not been assessed. Here, we used genetic stock identification to evaluate the effect of tributaries on population diversity of Yellowstone cutthroat trout ( Oncorhynchus virginalis bouvieri ) occupying the main stem Snake River, Wyoming, USA. We found that the main stem relied almost entirely on tributaries for demographic support, but main stem composition varied spatially among river sections. Distance between habitat patches, catchment area, and groundwater availability acted in concert to determine the contribution of specific tributaries to the main stem, but contributions were ultimately modulated by habitat connectivity. We also found evidence for multi-scale spatial structure in tributary contributions, providing insight into untested drivers of main stem river population diversity. Our results demonstrate how spatially discrete and distributed riverscape attributes influence population diversity at broader spatial scales, illustrating how ecosystem resilience emerges from the dynamic, two-way exchange of individuals and energy across habitat networks. Management plans for large rivers that address the ecological contributions of tributaries may be needed to achieve optimal outcomes. Similarly, conservation strategies that exclusively focus on headwater streams may fail to capture the broader habitat requirements necessary to maintain robust cold-water fish populations and associated recreational fisheries, particularly under global environmental change.

Wyoming↗

USGS2026

High-resolution magnetic survey using an unoccupied aerial vehicle to constrain buried lava flow geometry, volume, and eruptive history of Little Cones, Crater Flat, Nevada

Magnetic surveys are an important tool used to augment geologic mapping in distributed volcanic fields. Using magnetic anomalies, it is possible to model the geometry of shallowly buried volcanic features, such as conduits, sills, and lava flows. This subsurface mapping is important for understanding eruption dynamics and emplacement of lava flows, and it sometimes reveals buried volcanoes no longer visible at the surface. These data are critical to better interpret the numbers, styles, and magnitudes of eruptions in distributed volcanic fields and their associated volcanic hazards. New advances in unoccupied aerial vehicles (UAVs) offer an attractive middle range of resolution and aerial coverage between ground-based magnetic surveys and aeromagnetic surveys. Here, we present the results of a UAV fluxgate magnetic survey of the Little Cones, Nevada, scoria cones, which have been the target of previous ground and aeromagnetic surveys. The magnetic anomalies at Little Cones are of interest because the surrounding alluvium conceals lava flows that erupted from Little Cones, making it very difficult to understand the volume and morphology of lava flows from geologic mapping alone. Nonlinear inversion of UAV-collected magnetic data were used to model the thickness and morphology of buried Little Cones’ lava flows with higher precision than achieved previously. The sequence of events and calculated flow characteristics are then interpreted. The total volume of Little Cones, including concealed lava flows, is approximately 0.016 cubic kilometer, and the initial sheet flow erupted in less than 24 hours. The findings presented herein demonstrate that UAV-based magnetic surveys are a reliable method of data collection and an efficient alternative to other survey methods, facilitating development of a three-dimensional perspective of distributed volcanic fields.

Nevada↗

USGS2026

Post-fire soil hydrologic response and recovery in northern California (USA)

Background Wildfires abruptly change landscapes by altering soil properties and vegetation cover. These changes are thought to reduce soil infiltration capacity, making landscapes susceptible to runoff and erosion. However, post-fire soil response is complex and likely varies across locations and time. Aims Here, we aim to understand regional post-fire soil response and recovery by tracking changes across different northern California (USA) lithology and vegetation types. Methods We conducted repeat in situ soil infiltration tests for 3 years post-fire at 31 burned and 10 unburned sites spanning the 2021 Dixie, 2020 LNU Lightning Complex, 2020 Walbridge and 2020 Glass fires. Key results Our two main findings are: (1) burned chaparral soils have increased hydraulic conductivity compared with unburned sites, and (2) infiltration rates return to pre-fire conditions within 3 years across most lithologies and vegetations. Conclusions Recovery might be generalizable by vegetation and lithology but differ regionally, making it important to identify meaningful hydrologic response units (HRUs). Multi-year studies with paired burned and unburned measurements can constrain the recovery timeline and provide information missed by observations solely of burned soils. Implications Understanding where, and for how long, soil remains susceptible to runoff and erosion can help prioritize areas and time periods most in need of mitigation.

California↗

USGS2026

Rapid earthquake magnitude classification via P-wave strains from borehole strainmeters and Distributed Acoustic Sensing

Distributed Acoustic Sensing (DAS) offers a promising approach for earthquake early warning (EEW) in settings where seismic networks are costly to maintain. By repurposing fiber-optic cables as dense strainmeter arrays, DAS enables real-time earthquake detection wherever those fibers are accessible. However, poor azimuthal coverage and challenges in estimating magnitude from strain measurements remain key hurdles in applying for earthquake monitoring. Here, we develop a machine learning method to distinguish large (M≥5.4) earthquakes from smaller ones within the first 4 seconds of a strain waveform after a P-wave arrival without determining location. Using ensemble decision tree models trained on borehole strainmeter data (3.5≤M≤7.1) and tested on onshore DAS waveforms (including the 2024 M7 Offshore Cape Mendocino earthquake), we find that low-frequency (0.2–0.5 Hz) continuous wavelet transform coefficients are the strongest predictors of magnitude, in addition to strain amplitude. Both DAS and borehole strainmeters effectively capture long-period strain signals, making these findings valuable for EEW systems. Our method shows high precision compared to the real-time EEW system, ShakeAlert®, supporting the position that DAS is a viable technology for earthquake monitoring and magnitude classification.

California↗

USGS2026

Cajon Pass and the southern San Andreas Fault System: Earthquake cycle stress accumulation and present-day loading

With over a century since the last major rupture affecting the wider Los Angeles region, tectonic stress has steadily built along the southern San Andreas and San Jacinto fault systems, raising concerns of an imminent large earthquake. Cajon Pass, located at the junction of these faults, represents a critical site for potential through-going ruptures in Southern California. We constructed new 4D earthquake cycle simulations using a 1000-year paleoseismic rupture history of the San Andreas Fault System (SAFS) to assess spatial and temporal variations in stress. A semi-analytic Fourier transform model was used to compute stress from 3D dislocations in an elastic plate overlying a Maxwell viscoelastic half-space, assuming a complete coseismic reset of resolved shear stress on ruptured elements. Results show highest stress accumulation north of Cajon Pass (∼1.8 MPa/100 years) due to greater slip rates, and lower rates south of Cajon Pass (∼1.0–1.5 MPa/100 years). By 2025, Coulomb stress is estimated at 2.8 MPa on the Mojave South (MOS) segment, 1.8 MPa on the North San Bernardino (NSB1) segment and 3.6 MPa on the San Jacinto Bernardino (SJB) segment. Segments accumulate stresses with characteristic ranges of pre-event stress interpreted as failure thresholds: 1.2–2.7 MPa for MOS, 0.4–1.6 MPa for NSB1, and 1.2–2.9 MPa for SJB. When the stress disparity between segments SJB and MOS narrows, the faults appear to rupture jointly, suggesting that stress levels may control how Cajon Pass acts as an earthquake gate. These results may inform seismic hazard assessments by linking stress evolution to fault interactions.

California↗

USGS2026

Predictions of anthropogenic background PFAS concentrations in soil and relation to bedrock lithology and groundwater quality

Detectable concentrations of per- and polyfluoroalkyl substances (PFAS) have been observed in soils in remote areas and presumably originate from atmospheric deposition. These anthropogenic background concentrations may enable some PFAS to leach to groundwater at levels that exceed regulatory criteria for drinking water. However, anthropogenic background soil concentrations and their connection to groundwater are not well characterized. We developed a boosted regression tree model to predict perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) concentrations in shallow soils across northern New England. Low soil pH was the most important predictor of elevated anthropogenic PFOS and PFOA concentrations in background soils, rather than potential PFAS sources, land use, or population density. Total organic carbon (TOC) was also an important predictor for PFOS soil concentrations. Model predictions indicate that 73% of the shallow soils within Maine, Vermont, and New Hampshire exceed New Hampshire’s Soil Remediation Standard for PFOS (0.5 ng/g) and 41% exceed the PFOA standard (0.4 ng/g). Analysis of soil model results and groundwater data suggests that areas with high soil pH are associated with higher groundwater detection frequencies, illustrating how areas with less retention in soil are, conversely, also areas with potentially greater groundwater vulnerability. Further analysis indicates that groundwater may be more vulnerable in calcareous lithologies.

Maine, New Hampshire, Vermont↗

USGS2026

Influence of new residential construction varying in housing density on bird species, human tolerance guilds, and communities

Human population growth and changing settlement patterns fuel the development of urban fringe lands worldwide, with implications for biodiversity. We conducted a 12-year study of birds in the fast-developing urban fringe lands of the central Puget Sound region, Washington, USA, to examine the effect of development configuration on birds. We hypothesized that lower-intensity conservation developments, compared to higher-intensity planned community developments, would benefit the overall bird community, as well as native forest birds (avoiders of human development) and avian generalist species (adapters to human development), but that higher-intensity planned community developments would benefit synanthropic species (exploiters of human development). We fit single-species and multi-species occupancy models to test these hypotheses. Consistent with our hypotheses, we found that a greater proportion of the overall community, avoiders, and adapters occupied lower-intensity conservation developments compared to higher-intensity planned community developments. However, we did not detect an effect of development type on the exploiter guild, and we found that species in the exploiter guild are variable in their response to the configuration of suburban developments. We also hypothesized that human tolerance guilds would be a useful predictor of individual species responses to development type. This hypothesis was somewhat supported: we found that, for avoiders, 87% of species in the guild had the same response to development type as the overall guild; for adapters, 63% had the same response as the overall guild, and for exploiters, only 44% had the same response as the overall guild. Our results indicate that the configuration of suburban developments can have a meaningful impact on bird communities, particularly on those species that are most sensitive to any level of development.

Washington↗

USGS2026

Geothermal district energy systems coupled with seasonal underground thermal energy storage: A U.S. techno-economic screening by climate and geology

In the United States, cooling-dominated commercial building loads can cause geothermal heat pump-based district energy systems to accumulate a long-term subsurface thermal imbalance, motivating the incorporation of seasonal underground thermal energy storage. We developed a transferable workflow to evaluate geothermal district systems that pair ground heat exchangers with seasonal underground thermal energy storage. Using standardized hourly loads for seven commercial buildings and a uniform cost framework, we simulated ten U.S. cities with a physics-based ground heat exchanger model, subsurface storage simulations, and economic assessment to isolate the roles of climate and hydrogeology. In cooling-dominated cities, underground thermal energy storage supplied the majority of annual cooling, cutting electricity use and summer peaks substantially while achieving levelized costs comparable to or below conventional chiller-boiler plants. In cooler climates, the storage share shrunk, required borefield size and costs rose, and levelized cost of energy increased nearly linearly with declining underground thermal energy storage fraction, indicating storage fraction as the primary economic lever. Sensitivity analysis showed capital risk dominated by borefield drilling and surface heating, ventilation, and air-conditioning and piping, with underground thermal energy storage costs secondary. This workflow provides a transparent foundation for site-specific design and screening of next-generation geothermal district energy systems.

Arizona, Illinois, Maryland, Michigan, Mississippi↗

USGS2026

Assessment of extreme subsurface hydrologic conditions captured during atmospheric river storms in the San Francisco Bay area (California, USA) with applications to shallow landslide early warning

An increase in soil pore water pressure is the typical trigger for the majority of landslides caused by rainfall. Atmospheric river storms, common to the west coast of North America during the winter season, can deliver landslide triggering rainfall resulting in severe impacts to coastal communities. Using a network of hydrologic monitoring stations situated within landslide-prone terrain in the San Francisco Bay area of California (USA), we assess the meteorologic conditions and resulting hydrologic and landslide response resulting from eight consecutive storm events that caused thousands of shallow landslides during the winter of 2022–2023. We find disparate hydrological responses and resultant degrees of observed landsliding ranging from < 1 landslide/km2 to 18 landslides/km2 at the monitoring sites that reflect the interplay and differences between rainfall delivery, subsurface hydrological characteristics, and geotechnical properties at each site. Antecedent soil moisture from both early season rainfall and the first storm in the sequence played a critical role in setting up some hillslopes for failure. Subsequent storms then generated elevated pore water pressures for several hours with associated landsliding. However, we find that the occurrence of widespread landsliding required not only sufficient pore water pressure magnitude in susceptible hillslopes, but also full and prolonged effective soil saturation throughout hillslope profiles. Landslides may still occur at lower values and durations of effective saturation but are likely to be less extensive regionally. We present these findings within the context of research directions and improvements to landslide early warning systems first suggested by researchers 40 years ago.

California↗

USGS2026

Localization of spatiotemporally heterogeneous subsurface flows using autoencoder-based deep learning framework for time-lapse self-potential tomography

Self-potential (SP) monitoring has emerged as a valuable method for characterizing subsurface hydrogeological features and processes due to its sensitivity to fluid-induced electrokinetic effects. Despite advancements in SP inversion, challenges remain in imaging groundwater dynamics from SP activities due to complex hydrological settings and transient noise. In this study, a deep learning autoencoder (AE)-based framework is proposed for the spatiotemporal localization of subsurface fluid movement from time-lapse SP tomography. Temporal segments of time-lapse numerical inversions were first derived from long-term SP monitoring conducted from a floodplain site in Oak Ridge, Tennessee, known for active hyporheic exchange. Subsequently, AE models based on vision transformer (ViT), convolutional long short-term memory (ConvLSTM), convolutional neural network, and temporal convolutional network were individually trained and compared on the SP tomography segments for reconstruction performance. Finally, the reconstruction error over time serves as an anomaly score to identify moments of active SP variation, whereas spatial distributions of errors within these moments are analyzed to image and localize regions associated with anomalous subsurface fluid movement. The results demonstrate that ConvLSTM- and ViT-AE are most capable for the localization task with contrasting error distributions and consistent delineation of anomalies. Applying the method to both SP arrays parallel and perpendicular to the stream produced consistent anomaly zones near a fault or karst feature, validating the robustness and generalization of the approach. These results demonstrate the potential of the proposed framework as a scalable and interpretable tool for spatiotemporal analysis of subsurface flow dynamics in complex hydrogeological systems.

Tennessee↗

USGS2026

Evaluating the U.S. Geological Survey’s earthquake shaking hazard forecasts and their implications on seismic risk

We analyze the last six update cycles of the long-term probabilistic earthquake shaking hazard forecast from the U.S. Geological Survey (USGS) and discuss the changes in hazard estimates from the 1996 to the latest 2023 update of the National Seismic Hazard Model (NSHM) for the conterminous U.S. We summarize how our understanding of earthquake hazards has evolved over the last two decades and quantify the implications of changing hazards on people, buildings, and lifeline infrastructure. The net effect of changes in hazard estimates, with reference to the mean hazard from the 2023 NSHM update that has 2% probability of exceedance in 50 years, suggests an overall increase in total geographic areas facing very strong shaking levels (modified Mercalli Intensity (MMI) of VII or more) when compared with any of the previous cycles. This increase puts ~159.2 million people (an increase from 111.8 million when using the 2018 NSHM), ~47.3 million residential buildings (an increase from 32.5 million with the 2018 NSHM), and ~$25.6 trillion of economic exposure of all buildings (an increase from $17.4 trillion when using the 2018 NSHM) at risk from earthquakes. We demonstrate that small changes in hazard estimates do not necessarily imply small changes in risk estimates. Therefore, changes in risk estimates can be used to highlight key USGS NSHM updates for improving risk mitigation efforts.

Earthquake Spectra Journal↗

USGS2026

Future water constraints on United States lithium mining under climate change

Lithium is necessary for low-carbon technologies that combat climate change, but lithium extraction is water-intensive. Changes in temperature and precipitation arising from climate change are altering water distribution, which could further strain supplies for new mines and industry, farms, and households. Here we explored how climate change, water use, and mining siting could impact lithium mining in the United States. We analyzed whether there would be sufficient water available to support the single existing and 22 proposed U.S. lithium mines at mid-century under four socioeconomic-climate scenarios and five climate models. Though dependent on socioeconomic-climate scenario, climate model, and lithium deposit type, available water supply in most subbasins would likely be unable to support new mines’ water demands, or even non-mining water demands from other sectors. Water scarcity could hinder the ability of the United States to produce enough lithium to meet domestic demand thereby necessitating higher imports.

conterminous United States↗

USGS2026

Geochemical, mineralogical, and isotopic evidence for multi-stage genesis of the Hicks Dome REE + Y-HFSE-fluorite deposit, Illinois, USA

Hicks Dome hosts breccias enriched in rare earth elements (REE), Y, Th, F, Ba, Ti, Nb, and Be, alongside spatially associated lamprophyre dikes (ca. 271 Ma). Hicks Dome is located within the Illinois–Kentucky Fluorspar District, which hosts fluorite, Pb–Zn, and barite resources. This study investigates the genetic relationships between Hicks Dome mineralization in breccias, alkaline magmatism, and Illinois–Kentucky Fluorspar District mineralization. Lamprophyre dikes are light REE–enriched with chondrite-normalized abundances decreasing from La to Lu. The Host Breccia exhibits middle and heavy REE–enriched patterns that mirror those of the principal REE–Th host minerals, including fluorapatite, xenotime, and thorite. Textural evidence suggests recrystallization of phosphates, sulfates, and Ti–Nb oxides in the Host Breccia. U–Pb geochronology constrains multiple mineralizing events, with ages of 277 ± 18 Ma from low-Th apatite interpreted as main-stage mineralization, and 121.6 ± 9.7 Ma from high-Th apatite indicating later overprinting. O–H–C stable isotope data provide evidence for multiple stages of fluid-rock interaction and fluid mixing: (1) early magmatic fluids dissolved limestone country rock, (2) mixing between magmatic fluids and basinal brines led to main-stage mineralization in the Host Breccia, and (3) late-stage mineralization occurred following mixing of meteoric water and basinal brine. These results indicate that heavy REEs, high field strength elements, and fluorine precipitated proximal to its alkaline magmatic source because of fluid–rock interactions and fluid mixing. Subsequent fluid mixing drove late-stage recrystallization and additional fluorite formation, a process that may be similar to mineralization in the Illinois-Kentucky Fluorspar District.

Illinois↗

USGS2026

Moment magnitude for small earthquakes in the Delaware basin of west Texas and southeast New Mexico, USA

The Delaware Basin region of west Texas and southeast New Mexico has become one of the most prolific regions of seismic activity in the continental United States due to widespread hydraulic fracturing and wastewater disposal injection. In response to the increased number of earthquakes in this region, rapid and accurate characterization of earthquake sources is necessary to understand the evolution of seismic activity and level of seismic hazard associated with these earthquakes. This study re-evaluates earthquake magnitudes, estimating moment magnitude (MW) for small earthquakes in the Delaware Basin using 1) moment-rate spectra derived from S-wave coda envelopes, and 2) a relative magnitude method that relies exclusively on the ratio of waveform amplitudes between highly correlated waveform pairs. The coda-envelope method produces accurate M W estimates for small earthquakes ( M 1.5 – 3) that are consistent with independent, waveform modeled moment magnitudes for events with M W > 3 . Using the relative amplitudes method to extend these M W magnitudes to many other events, we successfully provide relative moment magnitude ( M W,rel ) values for 81% of the Texas Seismological Network catalog in the Delaware Basin region, and 45% of the USGS Induced Seismicity Project’s catalog of events in southeast New Mexico. The adoption and integration of the calibrated M W,rel method with current magnitude estimation methods offers valuable insights into the relationships between local and moment magnitude and will contribute to improved characterization of widespread induced seismicity.

New Mexico, Texas↗

USGS2026

Restoring dryland water cycles for precipitation feedback and climate stability; a review

Drylands across the globe are experiencing intensifying water scarcity, land degradation, and hydroclimatic extremes. This review integrates evidence from multidecadal field studies, hydrologic monitoring, geomorphic and ecological assessments, remote sensing, and land–atmosphere science to evaluate how restoration influences key components of the terrestrial water cycle. Low-tech natural infrastructure in dryland streams (NIDS)—including check dams, leaky weirs, one-rock dams, and gabions—has emerged as a promising but under-synthesized nature-based solution for restoring hydrologic function in these environments. We describe the mechanisms through which these interventions modify runoff detention, infiltration, sediment and alluvial storage, shallow-groundwater recharge, vegetation recovery, and surface-energy partitioning, and we summarize outcomes across diverse dryland settings. Findings consistently show increased water residence time, enhanced soil-moisture storage, expanded riparian vegetation, extended flow duration, and shifts toward greater latent-heat flux—producing localized cooling and strengthened ecohydrological feedbacks. Building on these localized effects, we articulate a hypothesis that links the spatial extent of restoration, the density of NIDS per unit drainage area, and the magnitude of the latent-to-sensible-heat contrast generated by wetter post-rainfall conditions. Specifically, we hypothesize that when NIDS are implemented at densities permitted by topography and across areas large enough to maintain elevated soil moisture after storm events, the resulting increases in latent heat flux, surface cooling, and boundary-layer moistening may enhance moisture convergence and boundary-layer development, potentially increasing the likelihood or stability of convective precipitation, analogous to how reductions in these processes have contributed to regional drought intensification. These land–atmosphere feedbacks remain untested at scale but represent an important research Frontier. By integrating hydrologic, geomorphic, ecological, and atmospheric perspectives, this review provides a comprehensive framework for considering how low-tech, landscape-scale interventions can strengthen watershed resilience and contribute to climate-relevant nature-based solutions.

Arizona↗

USGS2026

PFAS mixture composition and internal exposure profiles shape biological responses under field-realistic exposure

Per- and polyfluoroalkyl substances (PFAS) occur as complex mixtures, yet mixture-dependent biological effects under environmentally realistic exposure conditions remain poorly understood. We conducted multiyear (2018, 2019, 2021) continuous-flow, field-based exposures of male fathead minnows ( Pimephales promelas ) using a low-PFAS reference well (REF; sum of measured PFAS (∑PFAS) 0.1–0.2 μg L –1 ) and PFAS-contaminated groundwater from a fire-training area (FTA) at Joint Base Cape Cod, Massachusetts. Dilution treatments enabled separation of concentration and mixture effects. Groundwater from well FTA1 was perfluorooctanesulfonate (PFOS) dominated (∑PFAS 10–31 μg L –1 ), whereas groundwater from well FTA2 had higher concentrations and was enriched in perfluorooctanoate (PFOA) and diverse precursors (∑PFAS ∼ 80 μg L –1 ). Despite comparable plasma ∑PFAS and PFOS in FTA1–100% and FTA2–50% on day-7, cumulative mortality reached approximately 35% in FTA2–50% and 17% in FTA2–100%, and secondary sex trait expression was reduced by approximately 65–80% relative to REF, whereas sperm motility effects were mixture- and time-dependent. Plasma PFAS profiles were dominated by perfluorohexanesulfonate (PFHxS), PFOS, and sulfonamide precursors. Liver transcriptomics from REF-acclimated fish revealed robust disruption of metabolic, mitochondrial, and endocrine pathways that link PFAS mixture chemistry and internal exposure profiles to organismal outcomes, and testis transcriptomics provided complementary insight into reproductive impairment. These results indicate that PFAS mixture composition and internal exposure profiles, including precursor-associated differences, are important determinants of ecotoxicological outcomes under field-realistic conditions.

Massachusetts↗