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Research about Maine

Source-linked reports with geographic coverage including Maine.

At least 19 recordsLinked to original sources

3D Elevation Program—Maine

Using 3D Elevation Program data, a modern illustration of each State's landscape showcases its elevation differences in vivid detail. Each State has been consistently adjusted and colorized according to its highest and lowest elevations, accentuating differences even in States with low relief. To further emphasize landforms, shaded relief has been added on top of the elevation colors.

Maine↗

Proposing sources for discrete groundwater discharges to patterned pools in three regional raised northern peat bogs

Raised northern peat bogs are generally assumed to be entirely precipitation-fed (ombrogenous), suggesting they are void of groundwater (minerogenous) inflow from underlying sediments. Patterned pools in raised bogs have often been attributed to surficial flow filling depressions along the peat surface that produces subtle differences in peat pore-water chemistry. However, we present evidence that certain patterned pools may be partially fed by localized upwelling of minerogenous groundwater from underlying glacial sediments in three northern peat bogs of Maine, USA. Underlying permeable glacial deposits, embedded in hydraulically confining glacio-marine clay deposits, were delineated using ground-penetrating radar and transient electromagnetic surveys. Paired point measurements of temperature and specific conductance (SpC) were surveyed around pools, and statistical relationships indicative of groundwater upwelling were established. Uncrewed aerial systems (UAS) thermal infrared (TIR) mapping was conducted, augmented by handheld TIR imaging, to examine characteristic groundwater temperatures in cold and warm seasons across pool surfaces. Surface water samples were acquired to assess the relationship between SpC / temperature signals and elevated iron and manganese concentrations that could be indicative of glacial aquifer sources. The combined datasets present evidence for localized upwelling in pools underlain by glacial structures, and the possibility of minerogenous groundwater contributions. Upwelling through the peat matrix is possibly partially facilitated by macropore, “peat pipe” features that serve as preferential flowpaths of varying lengths to the surface. Such upwelling could drive a positive feedback loop where elevated concentrations of ionic constituents repeatedly dilate the peat matrix and/or terminal electron acceptors enhance anaerobic respiration and microbial activity, accelerating the humification of peat around patterned pools and potentially magnifying carbon loss.

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Life-history stages and behavior influence demographic classification of moose captured on remote cameras

Obtaining accurate information on demographic states, such as the age and sex classes of animals, is an important step for monitoring wildlife populations. Traditionally, demographic data are collected from harvest, aerial surveys and telemetry studies. However, these methods can be expensive, limited to small spatial scales, or biased due to human behavior. Remote cameras have become a mainstay for studying and monitoring wildlife as they are relatively inexpensive, can be deployed over large spatial scales, and effort can be accounted for during surveys. For some species, a variety of demographic information, such as sex and age classes, can be obtained from pictures. Moose Alces alces are a photogenic species found across boreal and semi-boreal forests of the Northern Hemisphere. Previous studies have used demographic data from remote cameras to estimate demographic parameters and population dynamics. A primary assumption is that these age and sex classes are accurately classified. However, numerous factors can influence the ability of observers to identify age and sex classes of moose captured on cameras. We used data from 84 cameras from a 3-year period (2021–2024) in northern Maine, USA, to evaluate how temporal, environmental, site-level, and endogenous factors influence observers' ability to classify age and sex classes of moose. Using Bayesian categorical regression models, we found that temporal variability, position and proximity of moose from cameras, and the behavior of moose influenced our ability to identify age and sex classes. This information can be used to decide which periods to use data for population modeling and how to design studies to reduce the amount of uncertainty associated with different age and sex classes. We anticipate that our approach could also be used for other species whose age and sex classes can be differentiated using remote cameras.

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Analyses of eye lens stable isotopes across ontogeny of trophically diverse freshwater salmonids

Ontogenetic niche shifts in fishes are nearly universal but remain poorly understood in many species despite being fundamentally important for the persistence, management, and conservation of fish populations, including those of vulnerable salmonids. Eye lens stable isotope analysis has proven useful in studying ontogeny in some marine species but has rarely been applied in freshwater fishes. We conducted among the first applications of eye lens stable isotope analysis in two salmonids, Arctic Charr ( Salvelinus alpinus ) and Brook Trout ( Salvelinus fontinalis ), in four North American lakes at the southern extent of the range of Arctic Charr (Maine, USA). Our goal was to determine if ontogenetic patterns varied between individuals and populations in ways that relate to differential vulnerability. Like studies in marine systems, we found patterns in lens isotopic values that agree with expected ontogenetic patterns to reach known adult trophic niches. Within lakes and individuals examined in this study, Arctic Charr appeared more dependent on pelagic resources than co-occurring Brook Trout through life. Using Bayesian hierarchical linear regressions, we found evidence that ontogenetic shifts in trophic position (measured by δ 15 N) of Arctic Charr may vary among lakes. Arctic Charr in some populations increased in trophic position through life (population lifetime δ 15 N posterior mean slope estimate = 1.01) while others showed no substantial changes (population lifetime δ 15 N posterior mean slope = 0.05), which may relate to differences in habitat and fish assemblage among our study lakes. Our study suggests that individual life stages and populations of salmonids are likely to respond to climate variability (e.g., basal resource shifts) differentially, which could warrant population and life-stage-specific management.

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Erosion potential and flood vulnerability of streams and stream crossings at Acadia National Park, Maine

Acadia National Park has had increases in the frequency and magnitude of precipitation in recent years, leading to increased flood flows, stream erosion, and costly infrastructure damage. To improve infrastructure management in a changing climate, the U.S. Geological Survey, in cooperation with the National Park Service, has developed multiple datasets that can help natural resource managers identify stream reaches and stream crossings that have the highest potential for erosion and flood damage within Acadia National Park. To develop these datasets, we first created a lidar-derived hydrography based on a 1-meter digital elevation model and then estimated peak flows at stream crossings and along the stream network using regional regression equations for Maine. We assessed the erosion potential of stream reaches by computing channel morphologic and hydrologic metrics associated with erosive power, such as stream steepness, topographic openness, and percent storage in the contributing watershed. Stream crossing flood vulnerability was assessed by comparing estimated peak flows to stream crossing conveyance capacities. Our results indicate that stream reaches in the headwaters of the Acadia National Park highlands such as Sargent, Penobscot, and Cadillac Mountain, have the highest erosion potential and generally coincide with reaches that have had erosion and infrastructure damage in the past. Stream crossings with the highest flood vulnerability are distributed throughout Mount Desert Island and Acadia National Park, especially south of Jordan Pond, north of Sargent Mountain, and surrounding Eagle Lake. Over a quarter of the total stream crossings have insufficient information to compute flood vulnerability and are often on the parts of the stream with the highest potential for erosion. The datasets allow users to identify stream reaches with the highest erosion potential, stream crossings that are most vulnerable to flood damage, and to highlight areas where supplemental field assessments could most effectively be completed.

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Is new actually better? A structural comparison of collaborative governance structures for the management of Atlantic salmon (Salmo salar) in Maine

Collaborative governance structures (CGS) have been increasingly adopted to address joint-jurisdictional management challenges. While the establishment of CGSs has been widely studied, their practical development has remained a conspicuous gap. CGSs are variable systems with several developmental stages (activation, collectivity, and institutionalization) which eventually lead to stabilization, decline, re-creation, or re-orientation. CGSs in decline may need to revisit earlier developmental stages if members choose to re-create or re-orient the structure. The Atlantic Salmon Recovery Framework (ASRF) was the CGS responsible for managing the Gulf of Maine Distinct Population Segment of Atlantic Salmon ( Salmo salar ) from 2011 until 2019 when it was replaced by the Collaborative Management Strategy (CMS) pilot program. The CMS was designed to address concerns identified in the ASRF; 1. slow and ineffective decision-making, 2. confusion surrounding leadership, and 3. low adaptive capacity. Building upon a previous evaluation of the ASRF, we used a parallel design to compare the structural components and participant perceptions of the two structures using a comparative case study methodology. The changes that occurred between the ASRF and the CMS constitute a re-creation, providing a unique opportunity to study this developmental phase of a CGS. The issues observed in the CMS may result from a misalignment between participant expectations and the current developmental stage of the re-created CGS. The CMS reorganized ASRF members and created roles for participants without direct management jurisdiction. The implementation of the CMS addressed many concerns identified in the ASRF while others remained (i.e., high membership uncertainty and confusion surrounding decision-making procedures). Formal leadership roles have created more equal representation, but participants still reported uncertainty regarding status and formalization of non-leadership roles.

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Postrelease movements of captive-reared adult Atlantic Salmon in two Maine rivers

Objective Atlantic Salmon Salmo salar remain at critically low levels in the United States, with the last remaining populations located in the state of Maine. In 2021, a pilot captive-rearing program, similar to a smolt-to-adult supplementation, was implemented to boost naturally spawning adults in support of recovery goals. Methods We conducted a 2-year acoustic telemetry study to track a subset of captive-reared salmon ( N = 270) that were released into the Penobscot and Machias rivers. We evaluated their postrelease movement patterns, dam passage, and site fidelity between the two rivers, years, and seasons. Results Atlantic Salmon that were released into the Penobscot River tended to overwinter, but their movement patterns varied between release years. In contrast, the Atlantic Salmon that were released into the Machias River were more likely to move directly downstream to the river exit. In addition, the fish that were released into the dammed Penobscot River frequently made multiple attempts to pass dams in both directions, often with passage delays and failures. Atlantic Salmon that were released during the summer displayed more exploratory movements than those that were released in the fall. Most Atlantic Salmon either left the river shortly after release (5–30 d) or the following spring (>120 d). Site fidelity was greater for the fall-released Atlantic Salmon (76%) than for the summer-released Atlantic Salmon (23%). Conclusions Overall, releasing salmon in the fall as sexually mature adults may increase site fidelity to the release reach, thereby enhancing the chances of successfully spawning in the wild.

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Seasonal movements of nonnative White Catfish in the Penobscot River estuary

Objective White Catfish Ameiurus catus has been introduced to coastal watersheds across the United States. In the Penobscot River, Maine, this species has become increasingly common in upstream habitats that have been made accessible by recent dam removals. We characterized the movements of White Catfish to understand the temporal variation in their movement patterns and contextualize these findings within the recent changes in watershed connectivity. Methods We captured and tagged 10 adult White Catfish (mean fork length = 271 mm) with acoustic transmitters in the lower Penobscot River in July 2022. The movements of the tagged fish were monitored through April 2023 with a large network of stationary receivers. Results The tagged catfish were detected up to 6 km upstream and 31 km downstream from the release site. The total distance that was traveled by individuals ranged from 0 to 154 km during the study. Fall and spring movements were associated with changes in river flow and water temperature, but fish were relatively stationary from December through March, when at least five individuals were assumed to have overwintered in lower river tributaries. Conclusion Our results show that individual White Catfish may move considerable distances within large river systems and that these movements are potentially facilitated by changing river conditions. Collectively, this study fills a long-standing knowledge gap about the movement ecology of this species, adds context to help explain a recent increase in observations within their introduced range, and shows how changes in river conditions may be used to predict when and where these fish will move within a tidal system.

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Seaward movements and mortality of Atlantic salmon Salmo salar L. kelts in the Penobscot River, Maine

The Atlantic salmon Salmo salar L. is an endangered species in the United States, but post-spawn downstream movements remain poorly understood. We conducted a 2-year acoustic telemetry study to characterize downstream movements and to quantify apparent mortality and downstream passage of post-spawn adults (kelts) in the Penobscot River, Maine, USA ( N = 112). The majority of tagged S. salar kelts (54%) exhibited a rapid movement seaward post-release instead of overwintering in the river. Salmo salar kelts that overwintered exhibited two patterns, distinguished by whether a dam was present or not. We found no relationship between post-release movement pattern, sex, body condition index or release year. Estimated apparent mortality rates were high (83%), with a greater probability of mortality occurring near the dams and release site. Apparent mortality rate did not differ by sex, body condition or year, but it was greater for individuals that moved out directly post-release and less for those that overwintered. While our results may suggest that overwintering in the river is a favourable pattern for survival, these results may be related to the closures of the designated downstream passages at the first two dams encountered by S. salar kelts. In fact, 55% of S. salar kelts passed a dam when the designated passages were closed. Altogether, these results may demonstrate the complex nature of downstream passage for S. salar kelts, particularly when having to navigate multiple dams.

Penobscot River↗

The story of the Penobscot River Ecology Mural: A 10-step process for scientists to create public art

Rivers are home to a wide variety of biota, including fishes, amphibians, reptiles, birds, freshwater mussels, aquatic insects, and microscopic organisms that fill unique niches to support broader ecosystem functions. While the general public may be aware of recreationally relevant biological life (e.g., fishes and insects to model flyfishing flies from), they may not be aware of more obscure yet important taxa. For example, freshwater mussels (Unionidae) improve water quality and clarity of rivers through filtration ( Vaughn, 2018), which is vital to riverine ecosystems. Despite this importance, lack of public knowledge about freshwater mussels is the second biggest challenge to their management ( Bouska et al., 2018). How do we best get the public to see value in underappreciated taxa to support their conservation? Public art installations can spread awareness about science effectively because they distill information for broader audiences, are eye-catching, and reach people through an emotional, aesthetic, and personal lens ( Thompson et al., 2023). While invertebrates are generally viewed by the public with fear and aversion ( Kellert, 1993) this can be ameliorated through interaction and learning, particularly for children ( Miller et al., 2025). Through cross-disciplinary collaboration between artists, scientists and the local community, we created the “Penobscot River Ecology Mural,” a scientifically informed artwork featuring underappreciated taxa like mussels and insects. In this article, we will elucidate the story of how we successfully made this piece through 10 steps, which provide a blueprint for future endeavors.

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Assessing American eel (Anguilla rostrata) distribution in a heavily dammed watershed using eDNA : The Penobscot River watershed, Maine, USA

Catadromous American eel ( Anguilla rostrata ) are native to Maine's Penobscot River watershed and historically have migrated through many of its tributaries prior to extensive damming. Recent restoration efforts, including dam removals, have improved connectivity in the lower reaches of the Penobscot River. Characterizing the extent of the American eel's distribution is important to inform restoration and identify extant barriers to migrations within the watershed. In the summer of 2023, we conducted eDNA surveys throughout the Penobscot River watershed to estimate the current distribution of the American eel and identify barriers to inland waters. Water samples were collected from 70 sites representing 37 rivers and streams; the presence or absence of American eel genetic markers within those samples was assessed using qPCR. We have shown that American eel are present in virtually the full extent of the area surveyed (68/70 sites). The results suggest that the majority of the main-stem dams may be passed by American eels at some level, with eel DNA being confirmed upstream of six dams. We confirmed the presence of American eels throughout the lower watershed with just 1 week of eDNA sampling and have highlighted this method for determining the species' access to habitat upstream of dams. The use of eDNA to sample locally (or regionally) for American eel may provide cost-effective information in data deficient areas and help assess the permeability of dam structures to diadromous species.

Maine↗

Water-resources inventory and assessment at Katahdin Woods and Waters National Monument

The U.S. Geological Survey, in cooperation with the National Park Service, prepared a water-resources inventory and assessment for Katahdin Woods and Waters National Monument (KAWW). This compilation includes published and publicly accessible hydrologic data and resource assessments of streams, rivers, ponds, lakes, wetlands, vernal pools, and groundwater in and near KAWW. It also includes reports and datasets summarizing attributes of KAWW’s hydrologic infrastructure, such as stream crossings, dams, wastewater discharge plants, groundwater monitoring wells, and U.S. Geological Survey streamflow-gaging stations. Descriptions of data and details of current limitations in available datasets are included. Wetland, groundwater, streamflow, and water-quality information are all limited. Hydrography data are available; however, there are limited ground-truth data. Accurate streamlines within KAWW were developed from light detection and ranging (lidar) as a part of this work. Hydrologic infrastructure information is available from multiple sources; however, differences exist among the datasets. Datasets are summarized in appendix 1.

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Anatectic origin of Mississippian spodumene-bearing pegmatites in western Maine during orogenic plateau collapse

Spodumene pegmatites are an important lithium source, but the processes and tectonic settings in which they form are poorly understood. The Rumford pegmatite district surrounding Plumbago Mountain, western Maine, is host to numerous spodumene pegmatites, including the Plumbago North pegmatite (a world-class spodumene resource). Competing petrogenetic models for these spodumene pegmatites include (1) highly fractionated melts of the Mooselookmeguntic igneous complex and (2) anatexis. We tested these hypotheses by constraining the geologic, magmatic, metamorphic, and tectonic history of the Plumbago Mountain area with detailed geologic mapping and U-(Th)-Pb geochronology. The Silurian Rangeley Formation records initial isoclinal folding prior to, and contact-related metamorphism synchronous with, the intrusion of the 417 ± 4 Ma Plumbago Mountain pluton. Peak amphibolite facies metamorphism and crustal melting occurred during the ca. 410 to 400 Ma Acadian orogeny. Pulsed emplacement of the Mooselookmeguntic igneous complex occurred between ca. 389 and 356 Ma. Cassiterite U-Pb dates of spodumene pegmatites (333–327 Ma) are ≥23 m.y. younger than nearby granitic plutons, strongly arguing against the fractional crystallization model. Metamorphic monazite and xenotime (346–328 Ma) and 330 to 308 Ma 40 Ar/ 39 Ar hornblende dates indicate metamorphism coeval with spodumene pegmatite emplacement, supporting anatectic models. Reheating, anatexis, and spodumene pegmatite emplacement occurred during collapse of the 380 to 330 Ma Acadian orogenic plateau. Lithium enrichment may be linked to one or more stages of partial melting of metasedimentary and plutonic rocks during the formation, tenure, and collapse of the Acadian altiplano and emphasizes the role of anatexis in producing spodumene pegmatites of economic significance.

Maine↗

Smallmouth bass (Micropterus dolomieu) and chain pickerel (Esox niger) identified as Atlantic salmon (Salmo salar) smolt predators in a reservoir system

Reservoir predation has been identified as a leading mortality source for smolts migrating through impounded river systems. We investigated smolt predation risk for an endangered Atlantic salmon ( Salmo salar ) population in the Weldon Dam reservoir in the Penobscot River, Maine, USA. In spring 2022, we characterized the fates of 390 tethered smolts. Smolts were exclusively predated by two predator species not native to the study area: chain pickerel ( Esox niger , n = 43) and smallmouth bass ( Micropterus dolomieu , n = 42). Using Cox-proportional hazard analysis, we estimated that 23% (95% CI = 15%–29%) of tethered smolts were expected to be predated within a one-hour deployment. Water temperature was the primary driver of predation risk as predation probability increased from 10% to 33% when temperature increased from 5 to 15 °C. Smolts also incurred above-average predation risk when they were within 40 m of shore. We demonstrate that non-native fish predation may drive patterns of high impoundment mortality and that risk is spatially and temporally heterogeneous within these systems. Collectively, this study offers direct evidence of species-specific predation on Atlantic salmon smolts and illuminates potential strategies to mitigate predation risk during reservoir migration.

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Local perceptions of marine conservation aquaculture for the restoration of native Atlantic salmon (Salmo salar) in Downeast, Maine

The entities responsible for the management of the endangered Gulf of Maine Distinct Population Segment of Atlantic salmon ( Salmo salar ) have partnered with a commercial aquaculture company to apply a novel conservation aquaculture program. This effort marks a major shift in management and has garnered mixed public reactions. Recent expansion of aquaculture in Maine has been a point of controversy as people and systems grapple with the social, environmental, and legal aspects of this burgeoning industry. Still, the use of aquaculture for conservation has shown some promise. In 2021, a questionnaire was administered to 850 households (response rate 27 %) in Maine via the Drop-off and Pick-up method. We examined differences and similarities in attitudes, beliefs, and knowledge about Atlantic salmon, hatcheries, and aquaculture between property owners in the town where the proposed net pens would be located (Cutler; n=73), and those in neighboring towns (n=152). The Potential for Conflict Index (PCI 2 ) was used to examine differences between the groups. Both groups held similar positive attitudes toward Atlantic salmon, endangered species conservation, freshwater hatcheries, and both commercial and conservation aquaculture. Both groups believe that “ Atlantic salmon should be protected, ” and that freshwater hatcheries and marine conservation aquaculture “ should be used for conservation. ” However, Cutler residents had less consensus and supported the use of freshwater hatcheries and marine conservation aquaculture less than other respondents. Community concerns have stalled efforts to move the project forward. Our research indicates there are several social concepts which may help to explain localized opposition to the project.

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Anatidae brood records in Maine during studies of Anas rubripes (American black duck), 1977–94

This report describes a compilation of brood observations for Anatidae species breeding in Maine during an 18-year period (1977–94) that were made by the U.S. Geological Survey’s Patuxent Wildlife Research Center while it was operated by the U.S. Fish and Wildlife Service. During four focused studies, variables affecting the declining Anas rubripes (Brewster, 1902) (American black duck, hereafter black duck) population were assessed. Broods were observed on seven geographical study sites within four study areas located in three of Maine’s five biophysical regions. For combined studies, 168 wetlands were monitored for broods. The 1,907 recorded broods were distributed among study areas: 185 in Dixmont, 241 in Cherryfield and Beddington, 411 in Moosehorn, Baring Unit and Edmunds Unit, and 849 in Aroostook County, Agricultural and Forested sites. Additionally, 221 broods were recorded in the 117-hectare Downing Bog wetland at the Cherryfield site during annual evening visits made between 1985 and 1991. Twelve Anatidae species, mostly black duck (676), Aix sponsa (Linnaeus, 1758) (wood duck; 265), Aythya collaris (Donovan, 1809) (ring-necked duck; 246), and Lophodytes cucullatus (Linnaeus, 1758) (hooded merganser; 163) were observed. Only 139 broods of Anas platyrhynchos (Linnaeus, 1758) (mallard) were found; all but 9 broods were at the Aroostook County, Agricultural site. Branta canadensis (Linnaeus, 1758) (Canada goose) broods were found at the Aroostook County, Agricultural site (58) and Moosehorn, Baring Unit (97). For the combined studies, 468 of 676 (69.2 percent) of black duck broods reached fledging age (Class IIc-III), whereas 93 of 139 (66.9 percent) of mallard broods reached fledging age. Black ducks used predominantly palustrine, emergent wetland; palustrine, forested wetland; and palustrine, scrub-shrub wetland. Of the 134 mallard broods observed at the Agricultural and Forested sites in Aroostook County, 130 (97.0 percent) were observed at the Agricultural site and 93 (71.5 percent) of them were recorded on two palustrine and two lacustrine, unconsolidated bottom class wetlands. Mean size of black duck, wood duck, and ring-necked duck broods in this report were similar to those reported from historic Maine data.

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No evidence hunting bait increases American black bear population growth in Maine, USA

American black bear ( Ursus americanus ) populations are increasing throughout much of North America. Use of multiple harvest methods, including hunting over bait, is intended to increase harvest success rates to meet harvest and population management objectives. However, black bear population growth can be influenced by food availability, and some speculate the use of bait may inadvertently increase bear abundance through food resource supplementation. We collected hair from black bears captured from 2005–2019 and a selection of potential food items, including those used as bait from 3 study areas in Maine, USA, with different levels of human influence. We used stable isotope analysis of the black bear hair and food item samples to evaluate the contribution of different identified food groups to the diet of individual bears. We found no evidence that greater contribution of human food resources, including bait, increased the number of cubs per litter for reproductive females or that human foods comprised a greater part of the diet in years when natural food resources were less abundant. Approximately 69% of black bear harvest occurred over bait. We found the probability of reproductive-age females to be harvested increased with average representation of human foods with high carbon enrichment (including bait) in their diet, but the relationship was weak, likely because of sampling constraints. Additionally, the probability of being harvested was greater in years when natural food resources were scarce. We conclude that bait is not available on the landscape in large enough amounts or for a sufficient amount of time in Maine to substantially influence female reproduction. Our results indicate hunting over bait is an effective tool in a state that is attempting to maintain a stable bear population via harvest, and dispels conjecture that hunting over bait may increase cub production and offset the intention of harvest.

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Peering into the eye of the sea lamprey: What can stable isotopes in lamprey eye lenses reveal about their life history?

The Atlantic sea lamprey ( Petromyzon marinus ) is an anadromous species which is a detritivore for years in freshwater, before metamorphosing into a juvenile and feeding parasitically at sea. However, their migratory patterns and marine ecology are poorly characterized. In other fishes, chemical analysis of calcified structures has aided in understanding their life history, but sea lamprey lacks these structures. Recent work using eye lenses suggests an alternate approach. Lenses are stable protein structures that accrue layers on their outer edge as they grow. We used stable isotope ratios (δ x Y) of hydrogen, carbon, nitrogen, and oxygen to explore lens layers and compare lenses to muscle. Returning adults were collected in May 2021 from the Penobscot (Maine), Connecticut (Massachusetts), and Susquehanna (Maryland) rivers. Lenses were dissected into three layers (surface, middle, core) and analyzed separately to investigate changes over time. We used mixed-effects models to assess differences in isotope ratios among lens layers. Isotope ratios changed from the core to the surface layer and δ 13 C were suggestive of a change from terrestrial to marine sources and δ 15 N showed an increase in trophic level as individuals grew, as would be expected when transitioning from detrital sources to marine prey. However, the isotope ratios of the outer lens layer generally did not correspond with the muscle, perhaps indicating a different temporal interval. These data suggest temporal accretion of lens proteins provide a record to explore sea lamprey ecology.

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