Research about Centre County, Huntingdon County, Pennsylvania

USGS2025

Landscape transcriptomic analysis detects thermal stress responses and potential adaptive variation in wild brook trout (Salvelinus fontinalis) during successive heatwaves

Extreme weather events, such as heatwaves, are becoming more frequent and intense as a result of climate change. Critically, such extreme weather events can be more important drivers of extirpation and selection than changes in annual or seasonal averages and they pose a particularly large threat to poikilothermic organisms. In this study, we evaluated the transcriptomic response of a coldwater adapted fish species, the eastern brook trout ( Salvelinus fontinalis ), to two successive heatwaves during July and August 2022. We sampled brook trout at eight time points from four streams ( N = 116 fish), sequenced mRNA from gill samples using TagSeq, and quantified expression levels of 32,670 unique transcripts. Multivariate analyses found that overall expression patterns in response to water temperature change were similar among streams. These analyses further detected groups of genes involved in immune response and oxygen carrier activity that were upregulated and downregulated respectively at higher water temperatures. We also detected 43 genes that were differentially expressed at different time points and followed the same expression pattern during the two heatwaves. Of these genes, 42 covaried with water temperature and most (27, 62.8 %) exhibited responses that varied by stream. Some of the differentially expressed genes, including heat shock proteins and cold-inducible RNA binding proteins, have been widely linked to temperature responses in experimental studies, whereas other genes we identified have functions that have not been well-studied in relationship to temperature or have unknown functions. This study shows the utility of landscape transcriptomic approaches to identify important biological processes governing wild organismal responses to short-term stressors. The results of this study can guide future investigations to identify phenotypic and genetic diversity that contribute to adaptive responses to heatwaves and improve predictions of how populations will respond to future climate change.

Pennsylvania↗

USGS2015

Water-budgets and recharge-area simulations for the Spring Creek and Nittany Creek Basins and parts of the Spruce Creek Basin, Centre and Huntingdon Counties, Pennsylvania, Water Years 2000–06

This report describes the results of a study by the U.S. Geological Survey in cooperation with ClearWater Conservancy and the Pennsylvania Department of Environmental Protection to develop a hydrologic model to simulate a water budget and identify areas of greater than average recharge for the Spring Creek Basin in central Pennsylvania. The model was developed to help policy makers, natural resource managers, and the public better understand and manage the water resources in the region. The Groundwater and Surface-water FLOW model (GSFLOW), which is an integration of the Precipitation-Runoff Modeling System (PRMS) and the Modular Groundwater Flow Model (MODFLOW-NWT), was used to simulate surface water and groundwater in the Spring Creek Basin for water years 2000–06. Because the groundwater and surface-water divides for the Spring Creek Basin do not coincide, the study area includes the Nittany Creek Basin and headwaters of the Spruce Creek Basin. The hydrologic model was developed by the use of a stepwise process: (1) develop and calibrate a PRMS model and steady-state MODFLOW-NWT model; (2) re-calibrate the steady-state MODFLOW-NWT model using potential recharge estimates simulated from the PRMS model, and (3) integrate the PRMS and MODFLOW-NWT models into GSFLOW. The individually calibrated PRMS and MODFLOW-NWT models were used as a starting point for the calibration of the fully coupled GSFLOW model. The GSFLOW model calibration was done by comparing observations and corresponding simulated values of streamflow from 11 streamgages and groundwater levels from 16 wells. The cumulative water budget and individual water budgets for water years 2000–06 were simulated by using GSFLOW. The largest source and sink terms are represented by precipitation and evapotranspiration, respectively. For the period simulated, a net surplus in the water budget was computed where inflows exceeded outflows by about 1.7 billion cubic feet (0.47 inches per year over the basin area); storage increased by about the same amount to balance the budget. The rate and distribution of recharge throughout the Spring Creek, Nittany Creek, and Spruce Creek Basins is variable as a result of the high degree of hydrogeologic heterogeneity and karst features. The greatest amount of recharge was simulated in the carbonate-bedrock valley, near the toe slopes of Nittany and Tussey Mountains, in the Scotia Barrens, and along the area coinciding with the Gatesburg Formation. Runoff extremes were observed for water years 2001 (dry year) and 2004 (wet year). Simulated average recharge rates (water reaching the saturated zone as defined in GSFLOW) for 2001 and 2004 were 5.4 in/yr and 22.0 in/yr, respectively. Areas where simulations show large variations in annual recharge between wet and dry years are the same areas where simulated recharge was large. Those areas where rates of groundwater recharge are much higher than average, and are capable of accepting substantially greater quantities of recharge during wet years, might be considered critical for maintaining the flow of springs, stream base flow, or the source of water to supply wells. The slopes of the Bald Eagle, Tussey, and Nittany Mountains are relatively insensitive to variations in recharge, primarily because of reduced infiltration rates and steep slopes.

Pennsylvania↗