Research about Aubrey Valley

USGS2024

Abundance estimates of Gunnison’s prairie dogs compared to the number of active burrows

Reliable estimates of prairie dog ( Cynomys spp.) population size and distribution are critical for assessing the status of prairie dogs and for selecting sites to reintroduce black-footed ferrets ( Mustela nigripes ). The density of active prairie dog burrows has commonly been used as an index of prairie dog abundance. Indices derived from active burrow counts were developed for black-tailed ( C. ludovicianus ) and white-tailed ( C. leucurus ) prairie dogs, but their efficacy has not been evaluated for all prairie dog species and studies affirming their validity with robust abundance estimators are few. We indexed or estimated the abundance of Gunnison's prairie dogs ( C. gunnisoni ) in the Aubrey Valley, Arizona, USA, in 2006 at 2 different time periods using 4 different methods—maximum above ground counts (MAGC), minimum number known alive (MNKA), capture-mark-recapture (CMR), and mark-resight—and compared these estimates to active burrow counts in 2005 and 2006. We found no positive relationship between active burrow counts and any abundance estimators. Mark-resight estimates of abundance were greater than the MNKA and were positively correlated with both the MNKA ( r 2 = 0.30) and CMR estimates ( r 2 = 0.49). Both CMR estimates and MAGC were typically below the MNKA and therefore biased low. Our results indicated that more rigorous estimation methods may be necessary to accurately estimate prairie dog abundance and assess habitat quality for ferret conservation.

Arizona↗

USGS2013

The innate immune response may be important for surviving plague in wild Gunnison's prairie dogs

Prairie dogs (Cynomys spp.) are highly susceptible to Yersinia pestis, with ≥99% mortality reported from multiple studies of plague epizootics. A colony of Gunnison's prairie dogs (Cynomys gunnisoni) in the Aubrey Valley (AV) of northern Arizona appears to have survived several regional epizootics of plague, whereas nearby colonies have been severely affected by Y. pestis. To examine potential mechanisms accounting for survival in the AV colony, we conducted a laboratory Y. pestis challenge experiment on 60 wild-caught prairie dogs from AV and from a nearby, large colony with frequent past outbreaks of plague, Espee (n = 30 per colony). Test animals were challenged subcutaneously with the fully virulent Y. pestis strain CO92 at three doses: 50, 5,000, and 50,000 colony-forming units (cfu); this range is lethal in black-tailed prairie dogs (Cynomys ludovicianus). Contrary to our expectations, only 40% of the animals died. Although mortality trended higher in the Espee colony (50%) compared with AV (30%), the differences among infectious doses were not statistically significant. Only 39% of the survivors developed moderate to high antibody levels to Y. pestis, indicating that mechanisms other than humoral immunity are important in resistance to plague. The ratio of neutrophils to lymphocytes was not correlated with plague survival in this study. However, several immune proteins with roles in innate immunity (VCAM-1, CXCL-1, and vWF) were upregulated during plague infection and warrant further inquiry into their role for protection against this disease. These results suggest plague resistance exists in wild populations of the Gunnison's prairie dog and provide important directions for future studies.

Arizona↗