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W.N. Batts

Publications and source records attributed to W.N. Batts.

20 records · Page 2Linked to original sources
USGS1987

Infectious hematopoietic necrosis virus detected by separation and incubation of cells from salmonid cavity fluid.

Infectious hematopoietic necrosis (IHN) virus is usually detected by inoculating susceptible cell cultures with cavity ("ovarian") fluid (CF) from spawning females. We identified additional adult carriers of virus in spawning populations of steelhead trout ( Salmo gairdneri ) and sockeye salmon ( Oncorhynchus nerka ) by collecting nonerythrocytic cells from CF samples by low-speed centrifugation, culturing the cells for at least 7 d at 15 °C, and then testing the culture medium for virus. Virus appeared in the cultured cells from some samples of CF that remained negative during incubation. In additional samples of CF from these species, the virus titer increased in cultured cells compared with the titer in the original CF sample. With chinook salmon ( O . tshawytscha ), no negative samples converted to positive during incubation, but the virus titer was retained in incubated CF cells, but not in cell-free CF.

Canadian Journal of Fisheries and Aquatic Sciences↗
USGS1987

Testing of male sockeye salmon ( Oncorhynchus nerka ) and steelhead trout ( Salmo gairdneri ) for infectious hematopoietic necrosis virus

Infectious hematopoietic necrosis (IHN) virus has been isolated only rarely from whole milt samples of male sockeye salmon ( Oncorhynchus nerka ). In 3 yr of testing, virus incidences in males ranged from 0 to 13% when milt was sampled but were 60–100% with spleen or kidney. When IHN virus was isolated from sockeye salmon milt at titers less than 3.00 log 10 plaque-forming units (pfu)/mL, the level of virus in the kidney or spleen exceeded 7.00 log 10 pfu/g. Higher rates of IHN virus isolation from kidney or spleen than from milt were also generally found in steelhead trout ( Salmo gairdneri ), although the differences were less pronounced than in sockeye salmon. Furthermore, virus was sometimes isolated from steelhead trout milt when the level of virus in kidney or spleen samples was very low, and was recovered from some milt samples when none was isolated from the corresponding spleen sample. When male salmonids are tested for IHN virus, kidney or spleen samples are superior to whole milt, but milt should be included for critical examinations.

Canadian Journal of Fisheries and Aquatic Sciences↗