Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci
Canada goose ( Branta Canadensis ) and harlequin duck ( Histrionicus histrionicus ) DNAs were digested with Sau 3AI, and size selected (300-700 bp) fragments were ligated into Bam HI-digested pBluscriptII KS + . The enrichment protocol of Ostrander et al . 1 was followed. The resulting libraries were screened using a [ƴ- 32 P]ATP end-labelled (CA) 20 oligonucleotides as a hybridization probe. Positive clones were sequenced using cycle-sequencing protocols (Epicentre Technologies, Madison, WI) and primers flanking the inserts. PCR primers were designed to amplify the repeat and yield amplification products of ≈100-200 bp. DNA samples were screened for variation at these loci using [ƴ- 32 P]ATP end-labelled primers. The products were resolved using 6% denaturing polyacrylamide gels and autoradiography.