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W. Cho

Publications and source records attributed to W. Cho.

2 recordsLinked to original sources

10,000 m under the sea: An overview of the HADES expedition to Kermadec Trench

The hadal zone of the world oceans (6000– 11,000 m) occupies <1% of the marine realm and is found almost exclusively in trenches but represents ~40% of the total ocean depth range. Jamison et al. (2010 & Jamison, 2015) have reviewed the current state of knowledge about the hydrology, physical characteristics, food supply, ecology and biodiversity of life in hadal trenches. This review concluded that, there appears to be a high level of endemism based on the few specimens collected from historical sampling efforts in the 1950s (Danish Galathea and Soviet Vitjaz expeditions), but because trenches are still largely unexplored there is a lot we do not know about the ecological structure and functioning of hadal environments. However, relatively recent advances in technology using remotely operated vehicles (ROV) and landers can help us explore hadal trenches in greater detail.

Conference Paper

A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth

The nonvirion (NV) protein of infectious hematopoietic necrosis virus (IHNV) has been previously reported to be essential for efficient growth and pathogenicity of IHNV. However, little is known about the mechanism by which the NV supports the viral growth. In this study, cellular localization of NV and its role in IHNV growth in host cells was investigated. Through transient transfection in RTG-2 cells of NV fused to green fluorescent protein (GFP), a nuclear localization of NV was demonstrated. Deletion analyses showed that the 32 EGDL 35 residues were essential for nuclear localization of NV protein, and fusion of these 4 amino acids to GFP directed its transport to the nucleus. We generated a recombinant IHNV, rIHNV-NV-ΔEGDL in which the 32 EGDL 35 was deleted from the NV. rIHNVs with wild-type NV (rIHNV-NV) or with the NV gene replaced with GFP (rIHNV-ΔNV-GFP) were used as controls. RTG-2 cells infected with rIHNV-ΔNV-GFP and rIHNV-NV-ΔEGDL yielded 12- and 5-fold less infectious virion, respectively, than wild type rIHNV-infected cells at 48 h post-infection (p.i.). While treatment with poly I:C at 24 h p.i. did not inhibit replication of wild-type rIHNVs, replication rates of rIHNV-ΔNV-GFP and rIHNV-NV-ΔEGDL were inhibited by poly I:C. In addition, both rIHNV-ΔNV and rIHNV-NV-ΔEGDL induced higher levels of expressions of both IFN1 and Mx1 than wild-type rIHNV. These data suggest that the IHNV NV may support the growth of IHNV through inhibition of the INF system and the amino acid residues of 32 EGDL 35 responsible for nuclear localization are important for the inhibitory activity of NV.

PLoS ONE