Tom Walsh | Geology Reports

USGS2024

Prokaryotic microbial ecology as an ecosurveillance tool for eukaryotic pathogen colonisation: Meiothermus and Naegleria fowleri

Naegleria fowleri has been detected in drinking water distribution systems (DWDS) in Australia, Pakistan and the United States and is the causative agent of the highly fatal disease primary amoebic meningoencephalitis. Previous small scale field studies have shown that Meiothermus may be a potential biomarker for N. fowleri . However, correlations between predictive biomarkers in small sample sizes often breakdown when applied to larger more representative datasets. This study represents one of the largest and most rigorous temporal investigations of Naegleria fowleri colonisation in an operational DWDS in the world and measured the association of Meiothermus and N. fowleri over a significantly larger space and time in the DWDS. A total of 232 samples were collected from five sites over three-years (2016-2018), which contained 29 positive N. fowleri samples. Two specific operational taxonomic units assigned to M. chliarophilus and M. hypogaeus, were significantly associated with N. fowleri presence. Furthermore, inoculation experiments demonstrated that Meiothermus was required to support N. fowleri growth in field-collected biofilms. This validates Meiothermus as prospective biological tool to aid in the identification and surveillance of N. fowleri colonisable sites.

Water Research↗

USGS2019

Viable long-term gas hydrate testing site confirmed on the Alaska north slope

In December 2018, data acquired in a Stratigraphic Test Well drilled from the 7-11-12 pad in the western part of the Prudhoe Bay Unit, Alaska North Slope confirmed the occurrence of two high-quality reservoirs fully saturated with gas hydrate. The drilling was the initial phase of a planned, three-well program designed to conduct an extended duration test of the response to gas hydrate reservoirs to controlled depressurization. The Stratigraphic Test Well (formally “PBU Hydrate-01”) was operated by the PBU Operator BP Exploration, (Alaska), Inc. (BPXA) using the Parker 272 drilling rig (Figure 1) through a Drilling Services Agreement executed with Petrotechnical Resources of Alaska (PRA) in association with a contract between NETL and PRA. The science program executed by BPXA was developed over a two-year period through extensive discussions and scientific evaluation undertaken by NETL, the Japan, Oil, Gas, and Metals, National Corporation (JOGMEC), the U.S. Geological Survey (USGS), and PRA. The effort also benefitted greatly from the support of the Alaska Department of Natural Resources (ADNR) and the PBU Working Interest Owners (WIOs).

Alaska↗