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Thomas Waldrop

Publications and source records attributed to Thomas Waldrop.

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The effects of swimming exercise and dissolved oxygen on growth performance, fin condition and survival of rainbow trout Oncorhynchus mykiss

Swimming exercise and dissolved oxygen (DO) are important parameters to consider when operating intensive salmonid aquaculture facilities. While previous research has focused on each of these two variables in rainbow trout Oncorhynchus mykiss , studies examining both variables in combination, and their potential interaction, are absent from the scientific literature. Both swimming exercise (usually measured in body lengths per second, or BL/s) and DO can be readily controlled in modern aquaculture systems; therefore, we sought to evaluate the effects of these variables, separately and combined, on several outcomes in rainbow trout including growth performance, fin health and survival. Rainbow trout fry (18 g) were stocked into 12 circular 0.5 m 3 tanks, provided with either high (1.5–2 BL/s) or low (approximately 0.5 BL/s) swimming exercise and high (100% saturation) or low (70% saturation) DO, and grown to approximately 1 kg. By the conclusion of the study, higher DO was independently associated with significantly ( p < .05) increased growth performance. Significant differences were not noted in other outcomes, namely feed conversion, condition factor and mortality, although caudal and right pectoral fin damage was associated with low oxygen and low swimming exercise treatments respectively. Cardiosomatic index was significantly higher among exercised fish. These results suggest that swimming exercise and DO at saturation during the culture of rainbow trout can be beneficial to producers through improved growth performance and cardiac health.

Aquaculture Research

The effects of swimming exercise and dissolved oxygen on growth performance, fin condition and precocious maturation of early-rearing Atlantic salmon Salmo salar

Swimming exercise, typically measured in body-lengths per second (BL/s), and dissolved oxygen (DO), are important environmental variables in fish culture. While there is an obvious physiological association between these two parameters, their interaction has not been adequately studied in Atlantic salmon Salmo salar . Because exercise and DO are variables that can be easily manipulated in modern aquaculture systems, we sought to assess the impact of these parameters, alone and in combination, on the performance, health and welfare of juvenile Atlantic salmon. In our study, Atlantic salmon fry were stocked into 12 circular 0.5 m 3 tanks in a flow-through system and exposed to either high (1.5–2 BL/s) or low (<0.5 BL/s) swimming speeding and high (100% saturation) or low (70% saturation) DO while being raised from 10 g to approximately 350 g in weight. Throughout the study period, we assessed the impacts of exercise and DO concentration on growth, feed conversion, survival and fin condition. By study's end, both increased swimming speed and higher DO were independently associated with a statistically significant increase in growth performance ( p < .05); however, no significant differences were noted in survival and feed conversion. Caudal fin damage was associated with low DO, while right pectoral fin damage was associated with higher swimming speed. Finally, precocious male sexual maturation was associated with low swimming speed. These results suggest that providing exercise and dissolved oxygen at saturation during Atlantic salmon early rearing can result in improved growth performance and a lower incidence of precocious parr.

Aquaculture Research

Non-lethal sampling for the detection of Myxobolus cerebralis in asymptomatic rainbow trout

We have described in previous reports (Schill et al., 1998) the development of a polymerase chain reaction (PCR) amplification of 18S ribosomal RNA for the detection of Myxozoan parasites. Oligonucleotide primers were developed by multiple alignment of Myxozoan sequence information and analysis by a custom-written computer program (PRIM). Candidate pairs of primer sequences were then analyzed for specificity by BLAST (Basic Local Alignment Search Tool). From these, a set of promising primers (MYXFWD and MYXREV) was chosen for further testing. These were chosen because they should direct detection of a number of Myxozoan species (Table 1). PCR using MXYFWD and MYXREV proved to be robust and relatively free of artifact products. Further, we were able to routinely detect Myxobolus cerebralis in fish tissues (Figure 1).

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Influence of temperature and substrate on infection rate, triactinomyxon production, and release duration from eastern tubifex worms infected with Myxobolus cerebralis

Salmonid whirling disease is caused by Myxobolus cerebralis , a metazoan parasite with a two host life cycle involving salmonid fish a an aquatic oligochaete, Tubifex tubifex (Wolf, Markiw and Hiltunen, 1986). Whirling disease has been reported in 22 U.S. states with the greatest losses occurring in the salmonid fisheries of western and Midwestern states. Although whirling disease is endemic in the eastern United States, serious documented losses to wild populations have not been reported. Two high priority research needs identified in 1996 were a better understanding of how worm and parasite populations might differ from different geographic areas and how environmental factors affect the various stages of whirling disease. To begin to address these research needs we established "eastern" populations of worms, parasite and fish hosts. This abstract will present data on the effects of temperature and substrate upon eastern T. tubifex worms infected with an eastern isolate of M. cerebralis . The influences of these abiotic factors upon the ability to infect the worms and subsequently their ability to produce waterborne triactinomyxons.

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