Geology topics

Stacy Kinsey

Publications and source records attributed to Stacy Kinsey.

4 recordsLinked to original sources

USGS2026

Detection of Naegleria fowleri in thermally impacted recreational waters of western United States national parks

Naegleria fowleri is a thermophilic free-living amoeba (FLA) and the causative agent of primary amoebic meningoencephalitis, posing public health risks in warm freshwater environments. This multiyear, multiagency study surveyed 40 thermally impacted recreational waters across five western United States national parks and recreation areas–Yellowstone National Park, Grand Teton National Park, Olympic National Park, Newberry National Volcanic Monument, and Lake Mead National Recreation Area–to assess N. fowleri presence, concentration, and associated environmental conditions. A total of 185 water samples were analyzed by qPCR and Sanger sequencing, revealing widespread detection of N. fowleri in 34% of samples with positive detections from Lake Mead, Yellowstone, and Grand Teton hot springs and thermally impacted waters, with concentrations ranging from 4.9 to 115.7 cells/L. Multiple codetections of N. fowleri with nonpathogenic species including Naegleria australiensis were identified, suggesting they may inhabit similar ecological niches in the natural systems in contrast to engineered systems. These findings indicate that N. fowleri is present in thermally impacted areas across the western United States and underscore the use of enhanced monitoring, public awareness, and risk management strategies in thermally influenced recreational waters.

Arizona, Nevada, Oregon, Washington, Wyoming↗

USGS2024

Naegleria fowleri detected in Grand Teton National Park hot springs

The free-living thermophilic amoeba Naegleria fowleri ( N. fowleri ) causes the highly fatal disease primary amoebic meningoencephalitis. The environmental conditions that are favorable to the growth and proliferation of N. fowleri are not well-defined, especially in northern regions of the United States. In this study, we used culture-based methods and multiple molecular approaches to detect and analyze N. fowleri and other Naegleria spp. in water, sediment, and biofilm samples from five hot spring sites in Grand Teton National Park, Wyoming, U.S.A. These results provide the first detections of N. fowleri in Grand Teton National Park and provide new insights into the distribution of pathogenic N. fowleri and other nonpathogenic Naegleria spp. in natural thermal water systems in northern latitudes.

Wyoming↗

USGS2020

Robotic environmental DNA bio-surveillance of freshwater health

Autonomous water sampling technologies may help to overcome the human resource challenges of monitoring biological threats to rivers over long time periods and large geographic areas. The Monterey Bay Aquarium Research Institute has pioneered a robotic Environmental Sample Processor (ESP) that overcomes some of the constraints associated with traditional sampling since it can automate water sample filtration and preservation of the captured material or homogenize it for immediate analyses in situ. The ESP was originally developed for marine environment applications. Here we evaluated whether the ESP can provide reliable, timely information on environmental (e)DNA detections of human and fish pathogens and introduced fishes at U.S. Geological Survey streamgage sites in freshwater rivers. We compared eDNA collected via ESP at high frequency (e.g., every 3 h) with manual eDNA collections collected at lower frequency (e.g., weekly). We found that water samples filtered and preserved by ESPs successfully detected the DNA of human pathogens, fish pathogens and introduced fishes. Both ESP and manually collected samples provided similar information about target DNA presence. We suggest that the greatest current benefit of the ESP is the cost savings of high frequency, bio-surveillance at remote or hard to access sites. The full potential of robotic technologies like the ESP will be realized when they can more easily execute in situ analyses of water samples and rapidly transmit results to decision-makers.

Idaho, Montana, Wyoming↗

USGS2019

Sources, fate, and flux of geothermal solutes in the Yellowstone and Gardner Rivers, Yellowstone National Park, WY

The total discharge and thermal output from the numerous hydrothermal features in Yellowstone National Park (YNP) can be estimated from the chloride (Cl) flux in the Madison, Yellowstone, Falls, and Snake Rivers. Monitoring the Cl flux in these four major rivers provides a holistic view of the hydrothermal output from YNP and changes in the Cl flux may indicate changes in geothermal or magmatic activity. In this study, the source, fate, and flux of geothermal solutes in the Yellowstone River and Gardner Rivers were determined. Beginning in 2012, the fluxes of geothermal solutes, including Cl, were determined at monitoring sites in the Yellowstone and Gardner Rivers downstream of geothermal inputs within YNP. A method was developed using specific conductance as a surrogate measure for solute concentrations at these monitoring sites. Combining continuous (15-min) specific conductance and discharge data, Cl and other geothermal solute fluxes were determined at both sites and approximately 32% of the Cl flux exiting YNP is from the Yellowstone River watershed. Synoptic sampling of river water and discharge measurements were performed during low-flow conditions of September 2014 allowed for the determinations of geothermal solute sources and their downstream fate. Thus, the contribution of geothermal solutes from the various geothermal areas at the downstream monitoring sites was quantified. The thermal features draining into Yellowstone Lake account for 34% of the Cl flux at the Yellowstone River monitoring site which is located approximately 5 km north of YNP. The Gardner River, which captures geothermal water from Mammoth Hot Springs, is responsible for 22% of the Cl at the Yellowstone River monitoring site. Because the Yellowstone River watershed is large and contains numerous thermal areas, knowing the source and fate of geothermal solutes is import baseline information that can be used to identify future changes in thermal activity.

Wyoming↗