Geology topics

Sara L. Caldwell Eldridge

Publications and source records attributed to Sara L. Caldwell Eldridge.

17 recordsLinked to original sources

USGS2026

Streamflow, water quality, and energy development characteristics and water-quality trends for selected sites in the Green River and Muddy Creek drainage basins, southwestern Wyoming, water years 2006–16

Energy resource development in southwestern Wyoming has been rapidly expanding since the early 2000s to include innovative technologies for extracting oil and gas and enhancing renewable energy. In response to concerns about the potential negative effects of energy development on natural resources, the Wyoming Landscape Conservation Initiative was created to implement a long-term, science-based program of assessing, conserving, and enhancing fish and wildlife habitats while facilitating responsible energy development through local collaborations and partnerships. The U.S. Geological Survey, in cooperation with the Bureau of Land Management, began monitoring streamflow and water quality as part of this initiative in 2006 at four U.S. Geological Survey streamgages within the Green River and Muddy Creek drainage basins to characterize the effects of energy development on streamflow and water quality in these areas. To quantify changes in water quality metrics, trend analyses for the 2006–16 water years were calculated and related to drainage basin characteristics including energy development (wells per square mile). Effects of energy development on water quality varied by watershed and the variability was driven primarily by the runoff efficiency and the geochemistry of the drainage basin. The New Fork River water chemistry was a calcium-bicarbonate type with low ionic strength and showed no statistically significant trends in water quality despite increased energy development. The Green River experienced moderate energy development, with significant upward trends in magnesium and alkalinity, indicating low sensitivity to development. Muddy Creek near Dad had minimal energy development and complex hydrogeochemical characteristics, showing mixed trends in water quality constituents. Muddy Creek near Baggs experienced moderate energy development, with significant upward trends in several water quality indicators indicating a response to the land disturbance associated with development. Overall, geochemistry and runoff efficiency influenced the vulnerability of water quality to energy development activities, highlighting the importance of drainage basin physical characteristics on vulnerability to development.

Wyoming↗

USGS2026

Sampling and analysis plan for the water-quality monitoring program in Lake Koocanusa and upper Kootenai River, Montana, water years 2022–23

The U.S. Geological Survey, in cooperation with the U.S. Environmental Protection Agency, collected water-quality samples and environmental data in Lake Koocanusa (also known as “Koocanusa Reservoir”), the Kootenai River, and the Tobacco River during water years 2022–23. The transboundary Lake Koocanusa is in southeastern British Columbia, Canada, and northwestern Montana, United States. It was formed by constructing Libby Dam on the Kootenai River 26 kilometers upstream from Libby, Montana. One of the lake sites and the Kootenai River site, in the Libby Dam tailwater (the outflow of the lake flow into the Kootenai River), were equipped with automated, high-frequency ServoSipper water samplers. At the lake site, these samplers were mounted to pontoon platforms during the summer, and a submersible ServoSipper sipper was deployed with ice buoys during the winter. Samples were automatically collected from multiple depths. At the Kootenai River site, these samplers were housed in the gage house. In water year 2022, discrete water-quality samples were collected every 4–6 weeks, year round, at all four lake sites in the Kootenai River between April and November. In water year 2023, discrete water-quality samples were collected at three lake sites and the Kootenai and Tobacco River sites every 4–6 weeks. The goal of this project was to collect multidepth, high-frequency vertical and temporal water-quality samples and data to understand the limnological and biological processes that control variations and trends in selenium concentrations and loads throughout Lake Koocanusa and in the Libby Dam tailwater at the southern end of the lake. This sampling and analysis plan documents the organization, sampling and data-collection scheme and design, pre- and post-collection processes, and quality-assurance and quality-control procedures of the Koocanusa/Kootenai water-quality monitoring program during water years 2022–23.

Montana↗

USGS2025

Metaproteomics and metagenomics reveal microbial pathways of organic matter degradation and methanogenesis in a marginally producing natural gas well

The expansion of natural gas production and utilization worldwide has led to the decline of many once-productive wells, eventually resulting in costly well-plugging and unused infrastructure. However, in areas like the Michigan basin, MI, where the majority of natural gas is biogenically produced, microbial communities could potentially be stimulated to generate additional methane, increasing gas supply and reducing the need to drill new wells. In this study, we performed metaproteomic, metagenomic, and geochemical analyses of Antrim Shale formation water from a marginally producing natural gas well to evaluate resident microbial community functions in the context of potential bioenergy production. Functional proteins involved in methanogenesis, degradation/catabolism (including organic matter degradation), biosynthesis, energy utilization, transmembrane transport, and stress response were among the most commonly identified groups. Three metagenome-assembled genomes (MAGs) were characterized, including Methanomicrobiaceae, Methanothrix , and Smithella . For each, the identified proteins involved in methanogenesis and the degradation of diverse organic compounds, strongly suggest their role in utilizing shale-derived organic matter. These findings provide an increased understanding of the microorganisms and their metabolisms generating natural gas in the Antrim Shale and establish a foundation for future stimulation efforts aimed at enhancing biogenic methane production in marginal gas wells.

Michigan↗

USGS2024

Naegleria fowleri detected in Grand Teton National Park hot springs

The free-living thermophilic amoeba Naegleria fowleri ( N. fowleri ) causes the highly fatal disease primary amoebic meningoencephalitis. The environmental conditions that are favorable to the growth and proliferation of N. fowleri are not well-defined, especially in northern regions of the United States. In this study, we used culture-based methods and multiple molecular approaches to detect and analyze N. fowleri and other Naegleria spp. in water, sediment, and biofilm samples from five hot spring sites in Grand Teton National Park, Wyoming, U.S.A. These results provide the first detections of N. fowleri in Grand Teton National Park and provide new insights into the distribution of pathogenic N. fowleri and other nonpathogenic Naegleria spp. in natural thermal water systems in northern latitudes.

Wyoming↗

USGS2023

Growth of coal mining operations in the Elk River Valley (Canada) linked to increasing solute transport of Se, NO3-, and SO42- into the transboundary Koocanusa Reservoir (USA-Canada)

Koocanusa Reservoir (KOC) is a waterbody that spans the United States (U.S.) and Canadian border. Increasing concentrations of total selenium (Se), nitrate + nitrite (NO 3 – , nitrite is insignificant or not present), and sulfate (SO 4 2– ) in KOC and downstream in the Kootenai River (Kootenay River in Canada) are tied to expanding coal mining operations in the Elk River Watershed, Canada. Using a paired watershed approach, trends in flow-normalized concentrations and loads were evaluated for Se, NO 3 – , and SO 4 2– for the two largest tributaries, the Kootenay and Elk Rivers, Canada. Increases in concentration (SO 4 2– 120%, Se 581%, NO 3 – 784%) and load (SO 4 2– 129%, Se 443%, NO 3 – 697%) in the Elk River (1979–2022 for NO 3 – , 1984–2022 for Se and SO 4 2– ) are among the largest documented increases in the primary literature, while only a small magnitude increase in SO 4 2– (7.7% concentration) and decreases in Se (−10%) and NO 3 – (−8.5%) were observed in the Kootenay River. Between 2009 and 2019, the Elk River contributed, on average, 29% of the combined flow, 95% of the Se, 76% of the NO 3 – , and 38% of the SO 4 2– entering the reservoir from these two major tributaries. The largest increase in solute concentrations occurred during baseflows, indicating a change in solute transport and delivery dynamics in the Elk River Watershed, which may be attributable to altered landscapes from coal mining operations including altered groundwater flow paths and increased chemical weathering in waste rock dumps. More recently there is evidence of surface water treatment operations providing some reduction in concentrations during low flow times of year; however, these appear to have a limited effect on annual loads entering KOC. These findings imply that current mine water treatment, which is focused on surface waters, may not sufficiently reduce the influence of mine-waste-derived solutes in the Elk River to allow constituent concentrations in KOC to meet U.S. water-quality standards.

Environmental Science and Technology↗

USGS2023

Spatiotemporal variations in copper, arsenic, cadmium, and zinc concentrations in surface water, fine-grained bed sediment, and aquatic macroinvertebrates in the upper Clark Fork Basin, western Montana—A 20-year synthesis, 1996–2016

The legacy of mining-related contamination in the upper Clark Fork Basin created an extensive longitudinal gradient in metal concentrations, extending from Silver Bow Creek to Lake Pend Oreille, Idaho. Downstream metal concentrations continue to decline, but, despite such improvements, the ecological health of much of the river remains uncertain. Understanding the long-term consequences of the Clark Fork River mining legacy may be supported by environmental monitoring techniques that include a holistic assessment of biological health or response to define organism exposure to complex contaminant mixtures and the consequences of such exposures. This report presents the spatiotemporal patterns of mining-related contaminants, copper, arsenic, cadmium, and zinc, in surface water, fine-grained bed sediment, and macroinvertebrate (aquatic insect) tissue in the upper Clark Fork from near Butte to Missoula, Montana. Overall, the patterns in water column sample concentrations observed in this study were consistent with previously observed trends, but bed sediment concentrations and concentrations of copper and arsenic varied more in tissue samples among sites. Trace element concentrations, especially copper, often exceeded the chronic aquatic life criteria and consistently exceeded the sediment probable effects level PEL for copper, particularly in the upper and middle river segments. The 20 years considered here were the wettest period since remediation started, and this increase in precipitation may have affected patterns in contaminant concentrations. Results of this study demonstrated the utility of a continued, comprehensive biomonitoring program to help guide and evaluate future environmental cleanup activities in the Clark Fork. Despite variation in defining complete restoration in these watersheds, using multiple lines of evidence in this study provided quantifiable measures of the timing and completeness of recovery relative to reference conditions. Successful recovery in the Clark Fork may benefit from an adaptive management strategy to continue collecting a comprehensive, multivariate dataset to evaluate whether established goals are being met and for subsequent adjustments and management, as needed.

Montana↗

USGS2023

Community for data integration 2019 project report

The U.S. Geological Survey Community for Data Integration annually supports small projects focusing on data integration for interdisciplinary research, innovative data management, and demonstration of new technologies. This report provides a summary of the 14 projects supported in fiscal year 2019 and outlines their goals, activities, and accomplishments. Proposals in 2019 were encouraged to address the optional disciplinary theme of biosurveillance of emerging invasive species and health threats.

Open-File Report↗

USGS2023

Sampling and analysis plan for the Koocanusa Reservoir and upper Kootenai River, Montana, water-quality monitoring program, 2021

In 2021, the U.S. Geological Survey will collect water-quality samples and environmental data from 3 sites in Koocanusa Reservoir and from 1 site in the Kootenai River. The transboundary Koocanusa Reservoir is in southeastern British Columbia, Canada, and northwestern Montana, United States, and was formed with the construction of Libby Dam on the Kootenai River 26 kilometers upstream from Libby, Montana. Two of the reservoir sites and the Kootenai River site, in the Libby Dam tailwater (the outflow of the reservoir flow into the Kootenai River), are equipped with automated, high-frequency ServoSipper water samplers. At the two reservoir sites, these samplers are mounted to pontoon platforms and automatically collect samples from multiple depths; a ServoSipper sampler was deployed at one site in 2019, and another ServoSipper sampler will be deployed at a second site in 2021. Discrete water-quality samples will be collected monthly at two depths at the river site and at two of the reservoir sites. The goal of this project is to collect multidepth, high-frequency vertical and temporal water-quality samples and data to understand the limnological and biological processes that control variations and trends in selenium concentrations and loads throughout Koocanusa Reservoir and in the Libby Dam tailwater at the southern end of the reservoir. This sampling and analysis plan documents the organization, sampling and data-collection scheme and design, pre- and post-collection processes, and quality-assurance and quality-control procedures.

Montana↗

USGS2021

Integrating environmental DNA results with diverse data sets to improve biosurveillance of river health

Autonomous, robotic environmental (e)DNA samplers now make it possible for biological observations to match the scale and quality of abiotic measurements collected by automated sensor networks. Merging these automated data streams may allow for improved insight into biotic responses to environmental change and stressors. Here, we merged eDNA data collected by robotic samplers installed at three U.S. Geological Survey (USGS) streamgages with gridded daily weather data, and daily water quality and quantity data into a cloud-hosted database. The eDNA targets were a rare fish parasite and a more common salmonid fish. We then used computationally expedient Bayesian hierarchical occupancy models to evaluate associations between abiotic conditions and eDNA detections and to simulate how uncertainty in result interpretation changes with the frequency of autonomous robotic eDNA sample collection. We developed scripts to automate data merging, cleaning and analysis steps into a chained-step, workflow. We found that inclusion of abiotic covariates only provided improved insight for the more common salmonid fish since its DNA was more frequently detected. Rare fish parasite DNA was infrequently detected, which caused occupancy parameter estimates and covariate associations to have high uncertainty. Our simulations found that collecting samples at least once per day resulted in more detections and less parameter uncertainty than less frequent sampling. Our occupancy and simulation results together demonstrate the advantages of robotic eDNA samplers and how these samples can be combined with easy to acquire, publicly available data to foster real-time biosurveillance and forecasting.

Idaho, Wyoming, Montana↗

USGS2020

Quantification of trace element loading in the upper Tenmile Creek drainage basin near Rimini, Montana, September 2011

The principle sources of trace elements entering upper Tenmile Creek, Montana, during September 2011, four trace metals and the metalloid arsenic, were identified and quantified by combining and analyzing streamflow data determined from tracer injection with trace-element concentrations and related water-quality data determined from synoptic sampling. The study reach was along upper Tenmile Creek, beginning downstream from the city of Helena’s diversion and extending 5,020 feet downstream. Results from the 2011 study, completed by the U.S. Geological Survey in cooperation with the Montana Department of Environmental Quality, were compared to results from a similar study conducted in 1998 to assess the effectiveness of mine reclamation and remediation work to reduce trace-element loading to upper Tenmile Creek, which has been ongoing throughout the drainage basin. Main-stem concentrations of most trace elements analyzed were generally greater in 1998 than in 2011. However, the State of Montana human-health criteria for total-recoverable cadmium and arsenic were exceeded in parts of upper Tenmile Creek, and concentrations of cadmium and zinc exceeded the acute aquatic-life criteria at all main-stem sites during both studies. Total-recoverable copper concentrations observed in 2011 exceeded the chronic aquatic-life criterion upstream from the Lee Mountain adit, whereas, in 1998, all sites exceeded the acute aquatic-life criteria. Direct comparison of loads from the 1998 and 2011 tracer studies were complicated by the differences in hydrologic conditions. Streamflow in 1998 was about 10 percent of the 2011 streamflow. The Lee Mountain Mine and Susie Lode adit were identified as major contributors of trace elements to upper Tenmile Creek in both studies. However, trace-element loading from the Lee Mountain Mine area was substantially reduced between 1998 and 2011. Total-recoverable loads of all trace elements showed substantial loss in 1998 but increased in 2011 downstream from the Susie Lode adit to the end of the study reach. This reach was one of the primary sources of trace-element loading to upper Tenmile Creek in 2011. This difference indicated that the streambed may act as a sink or a source for trace elements, depending on hydrologic conditions.

Montana↗

USGS2019

Contaminant concentrations in sediments, aquatic invertebrates, and fish in proximity to rail tracks used for coal transport in the Pacific Northwest: A baseline assessment

Railway transport of coal poses an environmental risk because coal dust contains polycyclic aromatic hydrocarbons (PAHs), mercury (Hg), and other trace metals. In the Pacific Northwest, proposed infrastructure projects could result in an increase in coal transport by train through the Columbia River corridor. Baseline information is needed on current distributions, levels, and spatial patterns of coal dust-derived contaminants in habitats and organisms adjacent to existing coal transport lines. To that end, we collected aquatic surface sediments, aquatic insects, and juvenile fish in 2014 and 2015 from Horsethief Lake State Park and Steigerwald National Wildlife Refuge (NWR), both located close to the rail line and within the Columbia River Gorge National Scenic Area. Two subsites in each area were selected: one close to the rail line and one far from the rail line. Detected PAH concentrations were relatively low compared to those measured at more urbanized areas. Some contaminants were measured at higher concentrations at the subsites close to the rail line, but it was not possible to link the contaminants to a definitive source. Trace metal concentrations were only slightly higher than background concentrations, but a few of the more sensitive benchmarks were exceeded, including those for As, Pb, and Se in fish tissue and fluoranthene, Cd, Cu, Mn, Ni, Zn, Fe, and As in sediments. At Horsethief Lake, Chinook salmon and yellow perch showed lower total mercury body burdens than other species, but PAH body burdens did not differ significantly among species. Differences in the species caught among subsites and the low number of invertebrate samples rendered food web comparisons difficult, but these data show that the PAHs and trace metals, including mercury, are accumulating in these wetland sites and in some resident organisms.

Washington↗

USGS2019

Annual variations in microcystin occurrence in Upper Klamath Lake, Oregon, based on high-throughput DNA sequencing, qPCR, and environmental parameters

Cyanobacteria-dominated blooms in Upper Klamath Lake, Oregon, create poor water quality and produce microcystins that may be detrimental to local wildlife and human health. Genetic tools, including high-throughput DNA sequencing and quantitative polymerase chain reaction (qPCR), have been shown to improve the identification and quantification of key groups associated with these blooms over more traditional techniques. We examined the seasonal and interannual variations in nutrient (nitrogen and phosphorus) concentrations between 2013 and 2014 to describe the relations between these factors and the growth dynamics of Aphanizomenon and toxigenic Microcystis as described by DNA sequencing and qPCR. Although total nutrients and chlorophyll a concentrations were similar between years, qPCR results showed the cyanobacterial populations to be 40 times larger in 2014 and indicated a large shift from an Aphanizomenon -dominant, low microcystin-level regime in 2013 to one dominated later in the season by microcystin-producing Microcystis in 2014. In both years, the transition from Aphanizomenon to Microcystis was coincident with a late-season increase in nitrite-plus-nitrate concentrations and in dissolved inorganic nitrogen to dissolved inorganic phosphorus (DIN:DIP) ratios. However, these increases did not explain the large interannual differences in total cyanobacteria abundance. Rather, we hypothesized that year-to-year differences in bioavailable phosphorus, which also manifested as lower total nitrogen to total phosphorus (TN:TP) ratios, were responsible.

Oregon↗

USGS2018

Nutrient loads in the Lost River and Klamath River Basins, south-central Oregon and northern California, March 2012–March 2015

The U.S. Geological Survey and Bureau of Reclamation collected water-quality data from March 2012 to March 2015 at locations in the Lost River and Klamath River Basins, Oregon, in an effort to characterize water quality and compute a nutrient budget for the Bureau of Reclamation Klamath Reclamation Project. The study described in this report resulted in the following significant findings: Total phosphorus (TP), total nitrogen (TN), 5-day biochemical oxygen demand (BOD5), and 5-day carbonaceous biochemical oxygen demand (CBOD5) loads, calculated using the U.S. Geological Survey LOADEST software package at the upper and lower boundaries of the Klamath Reclamation Project, indicated higher loads at the upper boundary on the southern end of Upper Klamath Lake upstream of the Bureau of Reclamation A Canal diversion compared to the lower boundary on the Klamath River downstream of Keno Dam. Accounting for the diversion of loads down A Canal, BOD5 and CBOD5 loads decreased between these two sites during irrigation season, indicating that the Klamath Reclamation Project is not a large source of oxygen-demanding material and that much of the oxygen demand at study site FMT, the northern boundary of the study area, has been expressed by the time the same water passes through site KRK, the southern boundary of the study area. An evaluation of the nutrient balance along the Klamath River flowpath from sites FMT to KRK indicated that, during irrigation season in the 3 years of the study period (March 2012–March 2015), more loads of TP, TN, BOD5, and CBOD5 were being diverted from the Klamath River than were being added to the Klamath River from the combination of Klamath Straits Drain, regulated point sources along the Klamath River, and internal loading from the bottom sediments in the river. By contrast, during non-irrigation seasons, more loads were added to the Klamath River than were diverted through Ady and North Canals, and this difference primarily was due to additional loads to the river from the Lost River Diversion Channel. At the Lost River Diversion Channel, BOD5 loads were higher during irrigation season than non-irrigation season in all three study years owing to the high concentrations of oxygen-demanding cyanobacterial biomass from the seasonal blooms of Aphanizomenon flos-aquae in the Klamath River and Upper Klamath Lake. The difference between the two seasons was particularly large in years 2 and 3, when the low flows of these two drought years resulted in smaller nonirrigation period loads than in year 1. CBOD5 loads also were higher during irrigation season in years 2 and 3 than during non-irrigation season, indicating that the largest oxygen demand was coming from senescence of Aphanizomenon flos-aquae cells that are present in the Klamath River during the summer. However, during irrigation season in year 1, CBOD5 loads were lower than in the non-irrigation season, which may indicate that at times high concentrations of ammonia or cellular organic nitrogen leaving Upper Klamath Lake contribute a large nitrogenous oxygen demand as well. The smallest loads were computed for the farthest upstream sites in the Lost River Basin, suggesting that the upper Lost River Basin does not contribute substantial loads of TP, TN, BOD5, and CBOD5 to the Klamath Reclamation Project. Median concentrations of BOD5 and CBOD5 were lowest among the upper Lost River Basin sites and highest at site PPD (however, this comparison is based on only four samples collected at site PPD over the 3-year study). Median concentrations of BOD5 and CBOD5 also were elevated at sites KSDH (6.60 and 4.70 milligrams per liter [mg/L], respectively) and KSD97 (4.47 and 3.45 mg/L, respectively). The highest maximum BOD5 and CBOD5 concentrations were reported at the Lost River Diversion Channel (39.0 and 26.5 mg/L, respectively) when water was flowing from the Klamath River toward the Klamath Reclamation Project, and site FMT (25.0 and 23.9 mg/L, respectively), the study site at the southern end of Upper Klamath Lake. Carbonaceous oxygen demand, as represented by CBOD5, typically dominated the composition of the samples at all sites. The highest concentrations of dissolved organic carbon were present at sites KSDH (the headworks of Klamath Straits Drain) and KSD97 (Klamath Straits drain before it enters the Klamath River), and PPD (outlet of Tule Lake). Median concentrations of TN and TP at the upper Lost River Basin sites in years 1 and 2 were variable, but site MCRV showed a smaller range of values in those years compared to the other upper Lost River Basins sites, and an overall lower median concentration during irrigation seasons in years 1 and 2, suggesting that Gerber Reservoir does not contribute high concentrations of nutrients to the Lost River during irrigation season. Total Maximum Daily Load (TMDL) load allocations for TP and TN in Klamath Straits Drain were exceeded in all three study years. BOD5 load allocations were exceeded in years 1 and 2, but not year 3. TMDL load allocations for TP were exceeded in the Lost River Diversion Channel for all 3 years. Load allocations for TN were exceeded in year 1, but not in years 2 and 3. BOD5 loads were less than the TMDL load allocation for all three study years. The dearth of samples collected at the Klamath Straits Drain just downstream of the Lower Klamath National Wildlife Refuge did not allow for direct assessment of the Klamath Straits Drain acting as a nutrient source or sink. TP, TN, BOD5, and CBOD5 loads estimated during the study period likely were smaller than long-term average conditions because of persistent drought conditions in the Upper Klamath Basin. The study results, therefore, fail to characterize loads from the Klamath Reclamation Project to the Klamath River that could be present in typical years, and suggest the need for load assessments during average or aboveaverage streamflow years.

California, Oregon↗

USGS2017

Using high-throughput DNA sequencing, genetic fingerprinting, and quantitative PCR as tools for monitoring bloom-forming and toxigenic cyanobacteria in Upper Klamath Lake, Oregon, 2013 and 2014

Monitoring the community structure and metabolic activities of cyanobacterial blooms in Upper Klamath Lake, Oregon, is critical to lake management because these blooms degrade water quality and produce toxic microcystins that are harmful to humans, domestic animals, and wildlife. Genetic tools, such as DNA fingerprinting by terminal restriction fragment length polymorphism (T-RFLP) analysis, high-throughput DNA sequencing (HTS), and real-time, quantitative polymerase chain reaction (qPCR), provide more sensitive and rapid assessments of bloom ecology than traditional techniques. The objectives of this study were (1) to characterize the microbial community at one site in Upper Klamath Lake and determine changes in the cyanobacterial community through time using T-RFLP and HTS in comparison with traditional light microscopy; (2) to determine relative abundances and changes in abundance over time of toxigenic Microcystis using qPCR; and (3) to determine relative abundances and changes in abundance over time of Aphanizomenon , Microcystis , and total cyanobacteria using qPCR. T-RFLP analysis of total cyanobacteria showed a dominance of only one or two distinct genotypes in samples from 2013, but results of HTS in 2013 and 2014 showed more variations in the bloom cycle that fit with the previous understanding of bloom dynamics in Upper Klamath Lake and indicated that potentially toxigenic Microcystis was more prevalent in 2014 than in years prior. The qPCR-estimated copy numbers of all target genes were higher in 2014 than in 2013, when microcystin concentrations also were higher. Total Microcystis density was shown with qPCR to be a better predictor of late-season increases in microcystin concentrations than the relative proportions of potentially toxigenic cells. In addition, qPCR targeting Aphanizomenon at one site in Upper Klamath Lake indicated a moderate bloom of this species (corresponding to chlorophyll a concentrations between approximately 75 and 200 micrograms per liter) from mid-June to mid-August, 2014. After August 18, the Aphanizomenon bloom was overtaken by Microcystis late in the season as microcystin concentrations peaked. Overall, results of this study showed how DNA-based, genetic methods may provide rapid and sensitive diagnoses for the presence of toxigenic cyanobacteria and that they are useful for general monitoring or ecological studies and identification of cyanobacterial community members in complex aquatic habitats. These same methods can also be used to simultaneously address spatial (horizontal and vertical) and temporal variation and under different conditions. Additionally, with some modifications, the same techniques can be applied to different sample types, including water, sediment, and tissue.

Oregon↗

USGS2014

Statistical analysis of the water-quality monitoring program, Upper Klamath Lake, Oregon, and optimization of the program for 2013 and beyond

Upper Klamath Lake in south-central Oregon has become increasingly eutrophic over the past century and now experiences seasonal cyanobacteria-dominated and potentially toxic phytoplankton blooms. Growth and decline of these blooms create poor water-quality conditions that can be detrimental to fish, including two resident endangered sucker species. Upper Klamath Lake is the primary water supply to agricultural areas within the upper Klamath Basin. Water from the lake is also used to generate power and to enhance and sustain downstream flows in the Klamath River. Water quality in Upper Klamath Lake has been monitored by the Klamath Tribes since the early 1990s and by the U.S. Geological Survey (USGS) since 2002. Management agencies and other stakeholders have determined that a re-evaluation of the goals for water-quality monitoring is warranted to assess whether current data-collection activities will continue to adequately provide data for researchers to address questions of interest and to facilitate future natural resource management decisions. The purpose of this study was to (1) compile an updated list of the goals and objectives for long-term water-quality monitoring in Upper Klamath Lake with input from upper Klamath Basin stakeholders, (2) assess the current water-quality monitoring programs in Upper Klamath Lake to determine whether existing data-collection strategies can fulfill the updated goals and objectives for monitoring, and (3) identify potential modifications to future monitoring plans in accordance with the updated monitoring objectives and improve stakeholder cooperation and data-collection efficiency. Data collected by the Klamath Tribes and the USGS were evaluated to determine whether consistent long-term trends in water-quality variables can be described by the dataset and whether the number and distribution of currently monitored sites captures the full range of environmental conditions and the multi-scale variability of water-quality parameters in the lake. Also, current monitoring strategies were scrutinized for unnecessary redundancy within the overall network.

Oregon↗

USGS2012

Water-quality data from Upper Klamath and Agency Lakes, Oregon, 2009-10

The U.S. Geological Survey Upper Klamath Lake water-quality monitoring program collected data from multiparameter continuous water-quality monitors, weekly water-quality samples, and meteorological stations during 2009 and 2010 from May through November each year. The results of these measurements and sample analyses, as well as quality-control data for the water-quality samples, are presented in this report for 14 sites on Upper Klamath Lake and 2 sites on Agency Lake. These 2 years of data demonstrate a contrast in the seasonal bloom of the dominant cyanobacterium, Aphanizomenon flos-aquae , that can be related to differences in the measured water quality and meteorological variables. Some of the significant findings from 2009 and 2010 are listed below. * Both 2009 and 2010 were characterized by two cyanobacteria blooms, but the blooms differed in timing and intensity. The first bloom in 2009 peaked in late June and at higher chlorophyll a concentrations at most sites than the first bloom in 2010, which peaked in mid-July. A major decline in the first 2009 bloom occurred in late July and was followed by a second bloom that peaked at most sites in mid-August and persisted through September. The decline of the weaker first bloom in 2010 occurred in early August and was followed by a more substantial second bloom that peaked between late August and early September at most sites. * Dissolved oxygen minima associated with bloom declines occurred approximately 2 weeks earlier in 2009 (mid-July) than in 2010 (early August). pH maxima associated with rapid bloom growth occurred in late June and again in mid-August in 2009 and in mid-July and late August in 2010. * In both years, the maxima for total phosphorus and total nitrogen concentrations coincided with the chlorophyll a maximum. The maxima for dissolved nutrient concentrations (orthophosphate, ammonia, and nitrite plus nitrate) coincided with the declines of the first blooms. * Total particulate carbon, total particulate nitrogen, and total particulate phosphorus concentrations were measured in 2009 only. The ratios of carbon to phosphorus and nitrogen to phosphorus in particulates were the highest of the entire season during the rapid growth phase of the first bloom and were the lowest of the season during the decline of the first bloom. These ratios increased with the onset of the second bloom in that year, but to a lesser degree. * Meteorological data show that 2009 was warmer (particularly in June and July), less windy, and more humid early in the season than 2010. The difference in water temperatures reflected the difference in air temperatures in that the lakes were warmer in 2009 than in 2010 starting in early May, when the sensors were deployed, through most of June. Water temperature peaked at a higher value in 2009, and there were more clear days in June 2009 than in June 2010.

Oregon↗

USGS2012

Spatial and temporal dynamics of cyanotoxins and their relation to other water quality variables in Upper Klamath Lake, Oregon, 2007-09

Phytoplankton blooms dominated by cyanobacteria that occur annually in hypereutrophic Upper Klamath Lake, Oregon, produce microcystins at concentrations that may contribute to the decline in populations of endangered Lost River ( Deltistes luxatus ) and shortnose ( Chasmistes brevirostris ) suckers. During 2007–09, water samples were collected from Upper Klamath Lake to determine the presence and concentrations of microcystins and cylindrospermopsins and to relate the spatial and temporal occurrences of microcystins to water quality and other environmental variables. Samples were analyzed for intracellular (particulate) and extracellular (dissolved) microcystins and cylindrospermopsins using enzyme-linked immunosorbent assays (ELISA). Samples contained the highest and most variable concentrations of microcystins in 2009, the year in which an earlier and heavier Aphanizomenon flos-aquae -dominated phytoplankton bloom occurred. Concentrations were lowest in 2008 when the bloom was lighter, overall, and delayed by nearly 1 month. Microcystins occurred primarily in dissolved and large (> 63 μm) particulate forms in all years of the study, and overall, concentrations were highest at MDT (the deepest site in the study) and HDB, although HDB was sampled only in 2007 and MDT was not sampled in 2008. Comparisons among daily median total microcystin concentrations; chlorophyll a concentrations; total, dissolved, and particulate nutrient concentrations; and nutrient ratios measured in 2009 and between 2007 and 2009 indicate that microcystin concentrations generally increase following the decline of the first A. flos-aquae -dominated bloom of each season in response to an increase in bioavailable nitrogen and phosphorus. Nitrogen fixation by A. flos-aquae early in the sample season appears to provide new nitrogen for growth of toxigenic Microcystis aeruginosa , whereas, later in the season, these species appear to co-exist. Understanding the ecological interactions between these species may be important for predicting periods of elevated cyanotoxin concentrations and has important implications for management of this lake.

Oregon↗