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S. Prager

Publications and source records attributed to S. Prager.

2 recordsLinked to original sources

Snow depth retrieval with an autonomous UAV-mounted software-defined radar

We present results from a field campaign to measure seasonal snow depth at Cameron Pass, Colorado, using a synthetic ultrawideband software-defined radar (SDRadar) implemented in commercially available Universal Software Radio Peripheral (USRP) software-defined radio hardware and flown on a small hexacopter unmanned aerial vehicle (UAV). We coherently synthesize an ultrawideband signal from stepped frequency 50-MHz subpulses across 600-2100-MHz frequency bands using a novel nonuniform nonlinear synthetic wideband waveform reconstruction technique that minimizes sweep time and completely eliminates problematic grating lobes and other processing artifacts traditionally seen in stepped waveform synthesis. We image seasonal snow across two transects: a 400-m open Meadow Transect and a 380-m forested transect. We present a surface detection algorithm that fuses data from LiDAR, global navigation satellite system (GNSS)/global positioning system (GPS), and features in the radargram itself to obtain high precision estimates of both snow and ground surface reflections, and thus total snow depth, represented as two-way travel time. The measurements are validated against independent ground-based ground-penetrating radar measurements with correlations coefficients as high as ρ = 0.9 demonstrated. Finally, we compare backscattered radar data collected by the UAV-SDRadar while hovering proximal to a known snow pit with in situ measured snow dielectric profiles and demonstrate imaging of snow stratigraphy.

Colorado↗

Evaluation of specimen preservatives for DNA analyses of bees

Large-scale insect collecting efforts that are facilitated by the use of pan traps result in large numbers of specimens being collected. Storage of these specimens can be problematic if space and equipment are limited. In this study, we investigated the effects of various preservatives (alcohol solutions and DMSO) on the amount and quality of DNA extracted from bees (specifically Halictidae, Apidae, and Andrenidae). In addition, we examined the amount and quality of DNA obtained from bee specimens killed and stored at -80 degrees C and from specimens stored for up to 24 years in ethanol. DNA quality was measured in terms of how well it could be PCR-amplified using a set of mitochondrial primers that are commonly used in insect molecular systematics. Overall the best methods of preservation were ultra-cold freezing and dimethyl sulfoxide, but these are both expensive and in the case of ultra-cold freezing, somewhat impractical for field entomologists. Additionally, dimethyl sulfoxide was shown to have adverse effects on morphological characters that are typically used for identification to the level of species. We therefore recommend that the best alternative is 95% ethanol, as it preserves bee specimens well for both morphological and molecular studies.

Journal of Hymenoptera Research↗