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Robert T. Anderson

Publications and source records attributed to Robert T. Anderson.

5 recordsLinked to original sources

Influence of dissimilatory metal reduction on fate of organic and metal contaminants in the subsurface

Geobacter become dominant members of the microbial community when Fe(III)-reducing conditions develop as the result of organic contamination, or when Fe(III) reduction is artificially stimulated. These results suggest that further understanding of the ecophysiology of Geobacter species would aid in better prediction of the natural attenuation of organic contaminants under anaerobic conditions and in the design of strategies for the bioremediation of subsurface metal contamination.

Hydrogeology Journal

Biogeochemistry: Hexadecane decay by methanogenesis

The potential for the biological conversion of long-chain saturated hydrocarbons to methane under anaerobic conditions has been demonstrated by using an enrichment culture of bacteria to degrade pure-phase hexadecane . The formation of methane in hydrocarbon-rich subsurface zones could be explained if a similar conversion of long-chain alkanes to methane were to take place in subsurface environments. If this process could be stimulated in the subsurface, it could be used to enhance hydrocarbon recovery from petroleum reserves . Parkes , however, questions the environmental significance of the enrichment-culture results 1 on the grounds that alkane conversion to methane is very slow and because sulphate-reducing and methanogenic bacteria might both be necessary for even this slow process to occur, restricting the conversion to specialized, unusual zones in sediments. Here we show that, on the contrary, subsurface bacteria can adapt to convert hexadecane to methane rapidly and in the absence of sulphate-reducing bacteria.

Nature

Anaerobic benzene oxidation in the Fe(III) reduction zone of petroleum-contaminated aquifers

The potential for anaerobic benzene oxidation in the Fe(III)-reduction zone of petroleum-contaminated aquifers was evaluated. Sediments were incubated under strict anaerobic conditions without any amendments in order to simulate in situ conditions. [ 14 C]Benzene was not oxidized to 14 CO 2 at most sites examined, which is consistent with previous studies that have found that benzene tends to persist in unamended, anaerobic aquifer materials and/or long periods of time are required in order to adapt the microbial population for benzene degradation. However, at one site located in Bemidji, MN, [ 14 C]benzene was oxidized to 14 CO 2 in unamended sediments without an apparent lag, suggesting that benzene was anaerobically degraded in situ. Benzene was not significantly oxidized in sediments from nearby Fe(III)-reducing sites nor in sediments collected from an uncontaminated background site in the same aquifer. Culturing and 16S rRNA-based molecular studies of the Bemidji aquifer demonstrated that while all sites contained similar numbers of Fe(III)-reducing microorganisms closely related to Geothrix fermentens , the site at which anaerobic benzene degradation was observed was greatly enriched with microorganisms in the family Geobacteraceae . This study provides the first data consistent with in situ anaerobic oxidation of benzene to carbon dioxide in the Fe(III)-reducing zone of a petroleum-contaminated aquifer and suggests that comparative studies on the size of the Geobacteraceae community in petroleum-contaminated aquifers might aid in the location of zones in which benzene degradation coupled to Fe(III) reduction is taking place.

Environmental Science & Technology

Evidence against hydrogen-based microbial ecosystems in basalt aquifers

It has been proposed that hydrogen produced from basalt-ground-water interactions may serve as an energy source that supports the existence of microorganisms in the deep subsurface on Earth and possibly on other planets. However, experiments demonstrated that hydrogen is not produced from basalt at an environmentally relevant, alkaline pH. Small amounts of hydrogen were produced at a lower pH in laboratory incubations, but even this hydrogen production was transitory. Furthermore, geochemical considerations suggest that previously reported rates of hydrogen production cannot be sustained over geologically significant time frames. These findings indicate that hydrogen production from basalt-ground-water interactions may not support microbial metabolism in the subsurface.

Idaho

Hydrologic and water-quality data for two small watersheds on Catoctin Mountain, North-Central Maryland, 1987-93

Hydrologic and water-quality data were collected from a precipitation-collection station and from two small watersheds on Catoctin Mountain, north-central Maryland, as part of investigations of acidic deposition and episodic acidification, and their effects on streamwater quality. Detailed descriptions of the site instrumentation in the watersheds, field data-collection techniques, and laboratory methods used to conduct the studies are included. Data that were collected on precipitation, throughfall, soil water, ground water, streamwater, and other surface and ground waters sampled during biannual synoptic surveys are given in tables. Data collected since October 1987 from one of the streamwater-quality monitoring sites and data collected since March 1988 from one of the ground-water quality monitoring sites are presented. Additional data collected since January 1987 from the precipitation station and data collected since June 1990 from all of the other water-quality monitoring sites are presented. Hydrologic data include tables of precipitation and throughfall quantities, streamflow, and synoptic measurements of ground-water levels. Selected hydrologic data are shown in graphs.

Maryland