Geology topics

N.P. Hitt

Publications and source records attributed to N.P. Hitt.

5 recordsLinked to original sources

USGS2013

Differential expression profiles of microRNA in the little brown bat (Myotis lucifugus) associated with white nose syndrome affected and unaffected individuals

First documented in New York State in 2006, white nose syndrome (WNS) quickly became the leading cause of mortality in hibernating bat species in the United States. WNS is caused by a psychrophilic fungus, Geomyces destructans. Clinical signs of this pathogen are expressed as a dusty white fungus predominately around the nose and on the wings of affected bats. Relatively new biomarkers, such as microRNAs (miRNAs) are being targeted as markers to predict the syndrome prior to the clinical manifestation. The primary objective of this study was to identify miRNAs that could serve as biomarkers and proxies of little brown bat health. Bats were collected from hibernacula that had tested positive and negative for WNS. Genetic sequencing was completed using the Ion Torrent platform. A number of miRNAs were identified from the liver as putative biomarkers of WNS. However, given the small sample size for each treatment, this data set has only coarsely identified miRNAs indicative of WNS, and further validation is required.

Open-File Report↗

USGS2011

Histologic, immunologic and endocrine biomarkers indicate contaminant effects in fishes of the Ashtabula River

The use of fish as sentinels of aquatic ecosystem health is a biologically relevant approach to environmental monitoring and assessment. We examined the health of the Ashtabula River using histologic, immunologic, and endocrine biomarkers in brown bullhead (BB; Ameiurus nebulosus) and largemouth bass (Micropterus salmoides) and compared fish collected from a reference site (Conneaut Creek). Seasonal analysis was necessary to distinguish differences in fish between the two rivers. Overall BB from the Ashtabula River had a lower condition factor and significantly more macrophage aggregates than those from the reference site. Reduced bactericidal and cytotoxic-cell activity was observed in anterior kidney leukocytes from both BB and largemouth bass from the Ashtabula River. Lower plasma thyroxine and triiodo-L-thyronine in both species in the Ashtabula River indicated disruption of the thyroid axis. Differences in physiological biomarker responses were supported by body burden chemical concentrations when data were analyzed on a seasonal basis. The use of two fish species added a level of rigor that demonstrated biological effects were not exclusive to a single species. The results provide strong evidence that contaminants have affected fish in the Ashtabula River, a Great Lakes Area of Concern, and provide a baseline by which to evaluate remediation activities.

Ohio↗

USGS2011

Hierarchical spatial structure of stream fish colonization and extinction

Spatial variation in extinction and colonization is expected to influence community composition over time. In stream fish communities, local species richness (alpha diversity) and species turnover (beta diversity) are thought to be regulated by high extinction rates in headwater streams and high colonization rates in downstream areas. We evaluated the spatiotemporal structure of fish communities in streams originally surveyed by Burton and Odum 1945 (Ecology 26: 182-194) in Virginia, USA and explored the effects of species traits on extinction and colonization dynamics. We documented dramatic changes in fish community structure at both the site and stream scales. Of the 34 fish species observed, 20 (59%) were present in both time periods, but 11 (32%) colonized the study area and three (9%) were extirpated over time. Within streams, alpha diversity increased in two of three streams but beta diversity decreased dramatically in all streams due to fish community homogenization caused by colonization of common species and extirpation of rare species. Among streams, however, fish communities differentiated over time. Regression trees indicated that reproductive life-history traits such as spawning mound construction, associations with mound-building species, and high fecundity were important predictors of species persistence or colonization. Conversely, native fishes not associated with mound-building exhibited the highest rates of extirpation from streams. Our results demonstrate that stream fish colonization and extinction dynamics exhibit hierarchical spatial structure and suggest that mound-building fishes serve as keystone species for colonization of headwater streams.

Oikos↗

USGS2011

Fish community and bioassessment responses to stream network position

If organisms move beyond the boundaries of local sampling units, regional metacommunity dynamics could undermine the ability of bioassessment studies to characterize local environmental quality. We tested the prediction that fish dispersal influences local fish community structure and bioassessment metrics as a function of site position within stream networks. We evaluated fish community data from the US Environmental Protection Agency's Regional Environmental Monitoring and Assessment Program in West Virginia, USA, to compare the influences of stream network position, ecoregion, basin, and stream size on local fish community composition. We assigned sites to 1 of 3 stream network positions: 1) main channels (MC, n = 12) encompassed streams with upstream catchment areas >200 km 2 , 2) mainstem tributaries (MT, n = 43) flowed into MC-sized confluences within 15 fluvial km, 3) headwater tributaries (HT, n = 31) lacked such riverine confluences within 15 fluvial km. MT and HT sites had similar upstream catchment sizes and landuse gradients, but species richness was greater in MT sites than HT sites, whereas MT and MC sites were not different in this regard. Three bioassessment metrics were greater in MT sites than HT sites (intolerant species richness, cyprinid species richness, benthic species richness), but a multimetric index of biotic integrity did not differ among stream network positions. Ordinations revealed that fish community composition was organized primarily by zoogeographic basin (Monongahela River basin, New River basin, Ohio River basin), ecoregion (Central Appalachian Plateau, Western Appalachian Plateau, Ridge and Valley), and stream size. Riverine specialists were more abundant in MT than HT sites and were more abundant in basins connected to the Ohio River than in basins isolated from the Ohio River by a large waterfall (New River). Our results suggest that contemporary dispersal among streams influences fish community composition over small spatial scales (10 1 km), historical dispersal constrained by zoogeographic barriers influences community structure over larger areas (10 2 km), and contemporary dispersal by fishes influences certain metrics commonly used in bioassessment programs.

Journal of the North American Benthological Societ↗

USGS2008

Evidence for fish dispersal from spatial analysis of stream network topology

Developing spatially explicit conservation strategies for stream fishes requires an understanding of the spatial structure of dispersal within stream networks. We explored spatial patterns of stream fish dispersal by evaluating how the size and proximity of connected streams (i.e., stream network topology) explained variation in fish assemblage structure and how this relationship varied with local stream size. We used data from the US Environmental Protection Agency's Environmental Monitoring and Assessment Program in wadeable streams of the Mid-Atlantic Highlands region (n = 308 sites). We quantified stream network topology with a continuous analysis based on the rate of downstream flow accumulation from sites and with a discrete analysis based on the presence of mainstem river confluences (i.e., basin area >250 km2) within 20 fluvial km (fkm) from sites. Continuous variation in stream network topology was related to local species richness within a distance of ???10 fkm, suggesting an influence of fish dispersal within this spatial grain. This effect was explained largely by catostomid species, cyprinid species, and riverine species, but was not explained by zoogeographic regions, ecoregions, sampling period, or spatial autocorrelation. Sites near mainstem river confluences supported greater species richness and abundance of catostomid, cyprinid, and ictalurid fishes than did sites >20 fkm from such confluences. Assemblages at sites on the smallest streams were not related to stream network topology, consistent with the hypothesis that local stream size regulates the influence of regional dispersal. These results demonstrate that the size and proximity of connected streams influence the spatial distribution of fish and suggest that these influences can be incorporated into the designs of stream bioassessments and reserves to enhance management efficacy. ?? 2008 by The North American Benthological Society.

Journal of the North American Benthological Societ↗