Geology topics

Michele R. Puffer

Publications and source records attributed to Michele R. Puffer.

5 recordsLinked to original sources

USGS2025

Do bighorn sheep use desert tortoise burrow spoil piles as mineral licks in southern California?

In a previous study, bighorn sheep ( Ovis canadensis ) were periodically photographed by trail cameras when they visited desert tortoise ( Gopherus agassizii ) burrows in the San Bernardino Mountains near Palm Springs, California, USA. The authors suggested that bighorn sheep may utilize the excavated spoil material from burrows as a mineral lick. To test that supposition, we collected soil samples from the spoil piles and undisturbed surface soil up to 1 m away at two desert tortoise study sites with bighorn sheep at the aforementioned site and another nearby site. We hypothesized that sodium concentrations would be greater in spoil than in nearby surface soil samples. Common major cations were quantified in soil samples using inductively coupled plasma-mass spectrometry following microwave-assisted digestion to solubilize the fraction of elements that could become biologically or environmentally available. Mean sodium concentrations were greater in burrow spoil samples than in undisturbed soil samples at both sites, likely due to aqueous leaching of sodium from surface to subsurface soil horizons. Although we did not collect evidence of geophagy by bighorn sheep, the behavior is well known. However, trail camera photographs of bighorn sheep with their head next to the entrance of tortoise burrows suggests that they may periodically utilize sodium and other elements brought to the surface by desert tortoises that are otherwise unavailable to bighorn sheep on the surface.

California↗

USGS2024

Aspects of the demography of a relict population of southwestern pond turtles (Actinemys pallida) in a West Mojave Desert stream in California

We studied Actinemys pallida (Southwestern Pond Turtle) in Amargosa Creek, near Palmdale, CA, from 1997 to 2023. The population in the upper creek was the focus of a mark–recapture study from 1997 to 2003 during monitoring required by a road-construction project. An estimated 193 (95% CI = 142–256) turtles were present in 1997 or recruited to the upper creek population between 1997 and 2003. Total abundance and recruitment declined after 2001, coincident with the onset of a multi-decadal megadrought. Turtles in upper Amargosa Creek are presumed to be extirpated because the creek dried up in the ensuing years. As part of a separate research project, we resurveyed the lower creek at Piute Ponds on Edwards Air Force Base from 2019 to 2023. As of 2023, there was a remnant breeding population of at least 22 turtles there. We did not find any marked turtles from the upper creek in the ponds. Only 2 populations of Southwestern Pond Turtles are known to survive in the Mojave Desert, one at Piute Ponds and another in the Mojave River.

California↗

USGS2024

Determining the distribution, status, and linkages of Agassiz's desert tortoise populations in the uplands surrounding the Coachella Valley

In support of the goals of the Coachella Valley Conservation Commission and the Bureau of Land Management, we performed surveys to determine the status, distribution, demographics, and possible genetic linkages of Agassiz’s desert tortoise (Gopherus agassizii) populations within the Coachella Valley Multiple Species Habitat Conservation Plan (CVMSHCP) area during a multi-decadal megadrought. Federal, state, and numerous parcels of privately owned conservation lands in the uplands around the periphery of the Coachella Valley were surveyed. Desert tortoise distribution data collected will be used to develop a species habitat model. These data will be overlaid with location data of flammable invasive plant species to inform fuels management and invasive plant control efforts in sensitive species habitat, as well as continue to identify high quality habitat and important desert tortoise linkage areas around the Coachella Valley. We also surveyed two established G. agassizii study plots – one at the Mesa Wind Energy Facility near Palm Springs, California and one at the Boyd Deep Canyon Desert Research Center. At those locations, we used resurveys and radio telemetry to assess habitat use, demography, and population status. We also used camera trapping at Deep Canyon to observe tortoise activities and behaviors in an unusual low elevation tortoise habitat.

California↗

USGS2023

High female desert tortoise mortality in the western Sonoran Desert during California’s epic 2012–2016 drought

We conducted population surveys for desert tortoises Gopherus agassizii at 2 nearby sites in the western Sonoran Desert of California, USA, from 2015-2018, during the driest ongoing 22 yr period (2000-2021) in the southwestern USA in over 1200 yr. We hypothesized that drought-induced mortality would be female-biased due to water and energy losses attributable to egg production during protracted periods of resource limitation. At the higher-elevation, cooler, wetter Cottonwood site from 2015-2016, the sex ratio of live adult tortoises was biased toward males and the sex ratio of tortoises estimated to have died during the intensified drought conditions from 2012-2016 was essentially even. At the lower-elevation, warmer, drier Orocopia site from 2017-2018, the sex ratio of live adult tortoises was biased toward males and the sex ratio of tortoises with estimated times of death from 2012-2016 was biased toward females. High female mortality at the Orocopia site may have resulted from the interaction of drought effects and the bet-hedging reproductive strategy of tortoises wherein they continue to produce clutches of eggs in drought years. Annual reproductive output results in an estimated loss of up to 13.5% of female tortoise body mass including over 0.20 l of water. Combined with dehydration during severe droughts, these losses may compromise their ability to survive droughts lasting more than 2 yr. The low tortoise density and high mortality of females observed may reflect reduced survival of tortoises near the southern edge of their range due to climate change, including protracted and intensified droughts.

Western Sonoran Desert↗

USGS2022

Injuries and abnormalities of the southwestern pond turtle (Actinemys pallida) in the Mojave River of California

The southwestern pond turtle ( Actinemys pallida ) is a semiaquatic turtle that occasionally spends time on land to bask, oviposit, make intermittent overland movements, and overwinter in terrestrial locations. Use of both aquatic and terrestrial environments exposes semiaquatic turtles to increased risk of injury or mortality from floods, predation attempts, and other environmental hazards (e.g., human activities such as vehicle strikes, etc.). We collected injury and morphological abnormality data from adult turtles at 3 study sites along the length of the Mojave River in San Bernardino County, California: 1 site on the upper half of the Mojave River (hereafter known as UHMRS) and 2 sites each on the lower half of the Mojave River (hereafter known as LHMRS). The studies were conducted when turtles were most active between May and October 1998–1999 and again from April to September 2016–2019. A total of 84 A. pallida were captured among all sites and all years. Seventeen percent ( n = 8) of the turtles captured at UHMRS exhibited shell abnormalities (natural variations in shell or bone morphology). Injuries (damage inflicted by force to the shell or body) occurred in 68% ( n = 26) of captured turtles at both the LHMRS sites combined and 78% ( n = 36) of turtles captured at the UHMRS alone. A total of 74% ( n = 62) of turtles had injuries at all sites combined. There was no statistical difference in the proportion of injured and noninjured turtles between the sexes for either the 2 LHMRS sites combined or the UHMRS. Mean carapace length was not significantly different between injured and noninjured turtles for these same sites. Injuries occurred in the majority of captured turtles at all sites and may be an indicator of the extent of threats facing these turtles.

Western North American Naturalist↗