Geology topics

Mark Waldrop

Publications and source records attributed to Mark Waldrop.

8 recordsLinked to original sources

USGS2023

Permafrost microbial communities and functional genes are structured by latitudinal and soil geochemical gradients

Permafrost underlies approximately one quarter of Northern Hemisphere terrestrial surfaces and contains 25–50% of the global soil carbon (C) pool. Permafrost soils and the C stocks within are vulnerable to ongoing and future projected climate warming. The biogeography of microbial communities inhabiting permafrost has not been examined beyond a small number of sites focused on local-scale variation. Permafrost is different from other soils. Perennially frozen conditions in permafrost dictate that microbial communities do not turn over quickly, thus possibly providing strong linkages to past environments. Thus, the factors structuring the composition and function of microbial communities may differ from patterns observed in other terrestrial environments. Here, we analyzed 133 permafrost metagenomes from North America, Europe, and Asia. Permafrost biodiversity and taxonomic distribution varied in relation to pH, latitude and soil depth. The distribution of genes differed by latitude, soil depth, age, and pH. Genes that were the most highly variable across all sites were associated with energy metabolism and C-assimilation. Specifically, methanogenesis, fermentation, nitrate reduction, and replenishment of citric acid cycle intermediates. This suggests that adaptations to energy acquisition and substrate availability are among some of the strongest selective pressures shaping permafrost microbial communities. The spatial variation in metabolic potential has primed communities for specific biogeochemical processes as soils thaw due to climate change, which could cause regional- to global- scale variation in C and nitrogen processing and greenhouse gas emissions.

The ISME Journal↗

USGS2022

The biogeography of relative abundance of soil fungi versus bacteria in surface topsoil

Fungi and bacteria are the two dominant groups of soil microbial communities worldwide. By controlling the turnover of soil organic matter, these organisms directly regulate the exchange of carbon between the soil and the atmosphere. Fundamental differences in the physiology and life history of bacteria and fungi suggest that variation in the biogeography of soil fungal and bacterial relative abundance could drive striking differences in carbon decomposition and soil organic matter formation across different biomes. However, a lack of global and predictive information on the distribution of these organisms in terrestrial 45 ecosystems has prevented the inclusion of soil fungal and bacterial relative abundance and the associated processes into global biogeochemical models. Here, we used a global scale dataset in the top soil surface (>3000 distinct observations of soil fungal and bacterial abundance) to generate the first quantitative and spatially high resolution (1km) explicit map of soil fungal proportion, defined as fungi/fungi + bacteria, across terrestrial ecosystems. We reveal striking latitudinal trends where fungal dominance increases in cold and high latitude environments with large soil carbon stocks. There was strong non-linear response of fungal 50 dominance to environmental gradient, i.e., mean annual temperature (MAT) and net primary productivity (NPP). Fungi and bacteria dominated in regions with low and high MAT and NPP, respectively, thus representing slow vs. fast soil energy channels, a concept with a long history in soil ecology. These high-resolution models provide the first steps towards representing the major soil microbial groups and their functional differences in global biogeochemical models to improve predictions of soil organic matter turnover under current and future climate scenarios

Earth System Science Data↗

USGS2021

Influence of permafrost type and site history on losses of permafrost carbon after thaw

We quantified permafrost peat plateau and post-thaw carbon (C) stocks across a chronosequence in Interior Alaska to evaluate the amount of C lost with thaw. Macrofossil reconstructions revealed three stratigraphic layers of peat: (1) a base layer of fen/marsh peat, (2) peat from a forested peat plateau (with permafrost) and, (3) collapse-scar bog peat (at sites where permafrost thaw has occurred). Radiocarbon dating revealed that peat initiated within the last 2,500 years and that permafrost aggraded during the Little Ice Age (ca. 250 – 575 years ago) and degraded within the last several decades. The timing of permafrost thaw within each feature was not related to thaw bog size. Their rate of expansion may be more influenced by local factors, such as ground ice content and subsurface water inputs. We found C losses due to thaw over the past century were up to 46% of the C available, but the absolute amount of C lost (kg m -2 ) was over 50% lower than losses previously described in other Alaskan peatland chronosequences. We hypothesize that this difference stems from the process by which permafrost aggraded, with sites that formed permafrost epigenetically (significantly later than most peat accumulation) experiencing less absolute C loss with thaw than sites that formed syngenetically (simultaneously with peat accumulation). Epigenetic peat from our site had lower C:N ratios as compared to Alaskan sites that have syngenetic peat. This difference could help predict the magnitude of C loss with thaw across a range or permafrost types and histories.

Alaska↗

USGS2021

Carbon fluxes and microbial activities from boreal peatlands experiencing permafrost thaw

Permafrost thaw in northern ecosystems may cause large quantities of carbon (C) to move from soil to atmospheric pools. Because soil microbial communities play a critical role in regulating C fluxes from soils, we examined microbial activity and greenhouse gas production soon after permafrost thaw and ground collapse (into collapse-scar bogs), relative to the permafrost plateau or older thaw features. Using multiple field and laboratory-based assays at a field site in interior Alaska, we show that the youngest collapse-scar bog had the highest CH 4 production potential from soil incubations, and, based upon temporal changes in porewater concentrations and 13 C-CH 4 and 13 C-CO 2 , had greater summer in situ rates of respiration, methanogenesis, and surface CH 4 oxidation. These patterns could be explained by greater C and N availability in the young bog, while alternative terminal electron accepting processes did not play a significant role. Field diffusive CH 4 fluxes from the young bog were 4.1 times greater in the shoulder season and 1.7–7.2 times greater in winter relative to older bogs, but not during summer. Greater relative CH 4 flux rates in the shoulder season and winter could be due to reduced CH 4 oxidation relative to summer, magnifying the importance of differences in production. Both the permafrost plateau and collapse-scar bogs were sources of C to the atmosphere due in large part to winter C fluxes. In collapse scar bogs, winter is a critical period when differences in thermokarst age translates to differences in surface fluxes.

Alaska↗

USGS2020

Getting to the root of plant‐mediated methane emissions and oxidation in a thermokarst bog

Vascular plants are important in the wetland methane cycle, but their effect on production, oxidation, and transport has high uncertainty, limiting our ability to predict emissions. In a permafrost‐thaw bog in Interior Alaska, we used plant manipulation treatments, field‐deployed planar optical oxygen sensors, direct measurements of methane oxidation, and microbial DNA analyses to disentangle mechanisms by which vascular vegetation affect methane emissions. Vegetation operated on top of baseline methane emissions, which varied with proximity to the thawing permafrost margin. Emissions from vegetated plots increased over the season, resulting in cumulative seasonal methane emissions that were 4.1–5.2 g m −2 season −1 greater than unvegetated plots. Mass balance calculations signify these greater emissions were due to increased methane production (3.0–3.5 g m −2 season −1 ) and decreased methane oxidation (1.1–1.6 g m −2 season −1 ). Minimal oxidation occurred along the plant‐transport pathway, and oxidation was suppressed outside the plant pathway. Our data indicate suppression of methane oxidation was stimulated by root exudates fueling competition among microbes for electron acceptors. This contention is supported by the fact that methane oxidation and relative abundance of methanotrophs decreased over the season in the presence of vegetation, but methane oxidation remained steady in unvegetated treatments; oxygen was not detected around plant roots but was detected around silicone tubes mimicking aerenchyma; and oxygen injection experiments suggested that oxygen consumption was faster in the presence of vascular vegetation. Root exudates are known to fuel methane production, and our work provides evidence they also decrease methane oxidation.

Alaska↗

USGS2019

Effect of permafrost thaw on plant and soil fungal community in the boreal forest: Does fungal community change mediate plant productivity response?

Permafrost thaw is leading to rapid shifts in boreal ecosystem function. Permafrost thaw affects soil carbon turnover through changes in soil hydrology, however, the biotic mechanisms regulating plant community response remain elusive. Here, we measured the response of fungal community composition and soil nutrient content in an intact permafrost plateau forest soil and an adjacent thermokarst bog using barcoded amplicon targeting ITS2 and 28S rRNA genes. Next, we used the soils from the permafrost plateau and the thermokarst bog as soil inoculum in a greenhouse experiment to measure whether shifts in fungal community and soil water level regulate plant productivity. Overall, we found that fungal community composition differed significantly between the thawed and intact permafrost sites, but soil nutrient content did not. Relative abundance of mycorrhizal fungal taxa decreased while relative abundance of putative fungal pathogens increased with permafrost thaw. In the greenhouse, we found that ecto- and arbuscular associated host plants had higher productivity in permafrost-intact soils relative to thawed soils. However, productivity of non-mycorrhizal tussock grass was more affected by soil water levels than soil communities. Our results suggest that fungal communities are crucial in mediating plant response to permafrost thaws inducing hydrology changes.

Alaska↗

USGS2019

Changes in the active, dead, and dormant microbial community structure across a Pleistocene permafrost chronosequence

Permafrost hosts a community of microorganisms that survive and reproduce for millennia despite extreme environmental conditions such as water stress, subzero temperatures, high salinity, and low nutrient availability. Many studies focused on permafrost microbial community composition use DNA-based methods such as metagenomic and 16S rRNA gene sequencing. However, these methods do not distinguish between active, dead, and dormant cells. This is of particular concern in ancient permafrost where constant subzero temperatures preserve DNA from dead organisms and dormancy may be a common survival strategy. To circumvent this we applied: (i) live/dead differential staining coupled with microscopy, (ii) endospore enrichment, and (iii) selective depletion of DNA from dead cells to permafrost microbial communities across a Pleistocene permafrost chronosequence (19 thousand years (K), 27K, and 33K). Cell counts and analysis of 16S rRNA gene amplicons from live, dead, and dormant cells revealed how communities differ between these pools, how they are influenced by soil physicochemical properties, and whether they change over geologic time. We found evidence that cells capable of forming endospores are not necessarily dormant and that members of class Bacilli were more likely to form endospores in response to long-term stressors associated with permafrost environmental conditions than members of Clostridia, which were more likely to persist as vegetative cells in our older samples. We also found that removing exogenous ‘relic’ DNA preserved within permafrost did not significantly alter microbial community composition. These results link the live, dead, and dormant microbial communities to physicochemical characteristics and provides insights into the survival of microbial communities in ancient permafrost.

Alaska↗

USGS2008

Carbon sequestration to mitigate climate change

Human activities, especially the burning of fossil fuels such as coal, oil, and gas, have caused a substantial increase in the concentration of carbon dioxide (CO2) in the atmosphere. This increase in atmospheric CO2 - from about 280 to more than 380 parts per million (ppm) over the last 250 years - is causing measurable global warming. Potential adverse impacts include sea-level rise; increased frequency and intensity of wildfires, floods, droughts, and tropical storms; changes in the amount, timing, and distribution of rain, snow, and runoff; and disturbance of coastal marine and other ecosystems. Rising atmospheric CO2 is also increasing the absorption of CO2 by seawater, causing the ocean to become more acidic, with potentially disruptive effects on marine plankton and coral reefs. Technically and economically feasible strategies are needed to mitigate the consequences of increased atmospheric CO2. The United States needs scientific information to develop ways to reduce human-caused CO2 emissions and to remove CO2 from the atmosphere.

Fact Sheet↗