Geology topics

Maria A. Diuk-Wasser

Publications and source records attributed to Maria A. Diuk-Wasser.

5 recordsLinked to original sources

USGS2025

Evaluation of SARS-CoV-2 antibody detection methods for wild Cervidae

Wildlife surveillance programs often use serological data to monitor exposure to pathogens. Diagnostic sensitivity and specificity of a serological assay quantify the true positive and negative rates of the diagnostic assay, respectively. However, an assay’s accuracy can be affected by wild animals’ pathogen exposure history and quality of the sample collected, requiring separate estimates of an assay’s detection ability for wild-sampled animals where an animal’s true disease status is unknown (referred to hereafter as sampling sensitivity and specificity). We assessed the sampling sensitivity and specificity of a Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) surrogate virus neutralization test (sVNT) and conventional virus neutralization tests (cVNT) to detect antibodies for ancestral and Omicron B.1.1.529 variants of SARS-CoV-2 in wild white-tailed deer ( Odocoileus virginianus ) and mule deer ( Odocoileus hemionus ). We studied the influence of sample collection method using paired blood samples collected in serum separator tubes and on Nobuto strips from the same animal. Mean estimates of sampling sensitivity and specificity ranged from 0.21–0.95 and 0.94–1.00, respectively, varying by sample collection method, host species, and SARS-CoV-2 variant targeted by the assay. Broadly, sampling sensitivity was estimated to be higher for 1) sera collected in tubes, 2) detecting pre-Omicron SARS-CoV-2 variants, and 3) sVNT relative to cVNT assays. Sampling specificity tended to be high for all tests. We augmented our study with SARS-CoV-2 spike protein sequences derived from sampling locations and times coincident with white-tailed deer captures, finding common amino acid mutations relative to the sVNT Omicron antigen variant. The mutations may indicate that the SARS-CoV-2 variants circulating in cervids from 2021 through 2024 may be better adapted to cervid hosts and more closely related to variants that circulated in humans prior to Omicron variants. We conclude our study with an inter-test comparison of sVNT results, revealing that 40 % inhibition is an optimal threshold for test positivity when testing deer sera for responses to Omicron variant B.1.1.529, compared to the 30 % inhibition recommended for ancestral variants.

Colorado, Illinois, Minnesota, New York, Pennsylva↗

USGS2024

Simple stated preference questions can enhance transdisciplinary projects: Linking perceived risks with willingness to spray and pay

Transdisciplinary projects can uncover crucial insights on people’s past and future risk-mitigation behavior. We focus on a novel risk context: increasing health threats from ticks on Staten Island, a New York City borough where the combination of high population density and extensive park systems and green spaces has resulted in a rise in locally-acquired tick-transmitted disease cases. We administered a knowledge, attitudes, and practices survey that additionally included simple economic stated preference questions about people’s willingness to spray tick pesticides in the future. We first analyze factors that are correlated with people’s perceptions of two types of risks: exposure to ticks and infection with Lyme disease. Next, we use the nonmarket valuation questions to test people’s willingness to spray and pay as a function of attributes of the hypothetical pesticides, including cost, effectiveness, and type. Across all model specifications, overall willingness to pay (WTP) to spray increases with increases in pesticide effectiveness, as well as with favorable pesticide type (organic). We uncover threshold pesticide effectiveness levels at which WTP to spray turns positive, and we find that lower pesticide effectiveness is required for organic pesticide. Finally, we test how perceived risks and various individual-specific characteristics correlate with WTP to spray. Combinations of higher perceived risks are linked both with higher WTP and lower breakeven pesticide effectiveness. Our work shows that a broad range of variables influence demand for self-protection actions, both directly and indirectly (through their effects on perceived risks). Such insights on people’s tradeoffs carry important policy implications, but they can be missed if economic information is either not elicited or elicited alone.

New York↗

USGS2024

Urban tick exposure on Staten Island is higher in pet owners

Over the past decade, Lyme and other tick-borne diseases have expanded into urban areas, including Staten Island, New York. While Lyme disease is often researched with a focus on human risk, domestic pets are also at risk of contracting the disease. The present study aims to describe differences in tick exposure, knowledge, attitude, and practices (KAP) between pet owners and non-owners, and to understand preventive strategies practiced by pet owners for themselves and their pets. We conducted KAP surveys via phone in 2020 and via face-to-face interviews in 2021, and we analyzed unique responses from 364 households on Staten Island. Pet owners were more likely to have ever found a tick on themselves or their household members (63%) than non-owners (46%) (p<0.001). Among pet owners, those who owned dogs (dog-only or both dog and cat owners) were more likely to have ever found a tick on their pets than cat-only owners (p<0.001). Compared with non-pet owners, pet owners were more likely both to know that ticks transmit Lyme disease (p<0.001) and to avoid gardening to reduce their tick exposure (p = 0.032), but they were less likely to wear protective clothing or adjust clothing (p = 0.013). Compared with cat owners who had never found a tick on their cats, cat owners who had ever found a tick on their cats were more likely to let their cats go outside (p<0.001). However, reported preventive measures on cats did not differ between pet owners who did and did not report tick exposure. The results indicate that encouraging pet owners to engage in preventative measures, both to protect themselves and their pets, is a potential avenue for healthcare providers and veterinarians to reduce risks from ticks.

New York↗

USGS2017

Lyme disease ecology in a changing world: Consensus, uncertainty and critical gaps for improving control

Lyme disease is the most common tick-borne disease in temperate regions of North America, Europe and Asia, and the number of reported cases has increased in many regions as landscapes have been altered. Although there has been extensive work on the ecology and epidemiology of this disease in both Europe and North America, substantial uncertainty exists about fundamental aspects that determine spatial and temporal variation in both disease risk and human incidence, which hamper effective and efficient prevention and control. Here we describe areas of consensus that can be built on, identify areas of uncertainty and outline research needed to fill these gaps to facilitate predictive models of disease risk and the development of novel disease control strategies. Key areas of uncertainty include: (i) the precise influence of deer abundance on tick abundance, (ii) how tick populations are regulated, (iii) assembly of host communities and tick-feeding patterns across different habitats, (iv) reservoir competence of host species, and (v) pathogenicity for humans of different genotypes of Borrelia burgdorferi . Filling these knowledge gaps will improve Lyme disease prevention and control and provide general insights into the drivers and dynamics of this emblematic multi-host–vector-borne zoonotic disease.

Philosophical Transactions of the Royal Society B:↗

USGS2016

Tick-, mosquito-, and rodent-borne parasite sampling designs for the National Ecological Observatory Network

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long-term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick-, mosquito-, and rodent-borne parasites through a continental-scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the data as well as archived samples to be made available to the research community. Insights generated by this sampling will advance current understanding of and ability to predict changes in infection and disease dynamics in novel, interdisciplinary, and collaborative ways.

Ecosphere↗