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M. A. Walden

Publications and source records attributed to M. A. Walden.

2 recordsLinked to original sources

Evaluating alternative methods for modeling trap efficiencies of out-migrating juvenile salmonids

Objective We aimed to compare two machine learning approaches—boosted beta regression (BBR) and beta mixed model forest (BMF)—to a Bayesian mixed-effects beta regression (BME) for the prediction of rotary screw trap (RST) efficiency for out-migrating juvenile salmonids from environmental covariates. Methods We identified two machine learning approaches that shared the ability to model overdispersed probabilities. We compared the BBR and BMF machine learning models to a BME model to evaluate precision in detection probability prediction and model performance on bias in parameter estimation. We tested our three candidate models using a simulation study to understand the specific advantages and disadvantages of each when the data set was increasingly sparse and the capture probabilities were realistically small. We then applied the models to a case study of RST data from the Klamath River in California, United States. Results The BME and BMF outperformed BBR in all simulated scenarios, although the BMF displayed poor explanatory power. In the case study, the BME and BMF identified environmental covariates that predicted RST efficiency. Conclusions Using the BME as a benchmark for comparing machine learning approaches to trap efficiency modeling, our simulations and case study demonstrated that the BMF performed well and is a viable modeling approach with strong predictive power. The BME model would be the preferred modeling approach when its strong explanatory power is desired.

North American Journal of Fisheries Management

Testosterone identifies hatchling sex for Mojave desert tortoises (Gopherus agassizii)

The threatened Mojave desert tortoise ( Gopherus agassizii ) exhibits temperature-dependent sex determination, and individuals appear externally sexually monomorphic until sexual maturity. A non-surgical sex identification method that is suitable for a single in situ encounter with hatchlings is essential for minimizing handling of wild animals. We tested (1) whether plasma testosterone quantified by enzyme-linked immunosorbent assay differentiated males from females in 0–3 month old captive hatchlings, and (2) whether an injection of follicle-stimulating hormone (FSH) differentially elevates testosterone in male hatchlings to aid in identifying sex. We validated sex by ceolioscopic (laparoscopic) surgery. We then fit the testosterone concentrations to lognormal distributions and identified the concentration below which individuals are more likely female, and above which individuals are more likely male. Using a parametric bootstrapping procedure, we estimated a 0.01–0.04% misidentification rate for naïve testosterone samples, and a 1.26–1.39% misidentification rate for challenged (post-FSH injection) testosterone samples. Quantification of plasma testosterone concentration from small volume (0.1 mL) blood samples appears to be a viable, highly accurate method to identify sex of 0–3 month old hatchlings and could be a valuable tool for conservation measures and investigation of trends and variation in sex ratios for in situ wild nests.

Scientific Reports