Geology Reports⌕ Search

Geology topics

Joseph W. Duris

Publications and source records attributed to Joseph W. Duris.

At least 19 recordsLinked to original sources

Predicted potential for aquatic exposure effects of per- and polyfluorinated alkyl substances (PFAS) in Pennsylvania’s statewide network of streams

Per- and polyfluoroalkyl substances (PFAS) are contaminants that can lead to adverse health effects in aquatic organisms, including reproductive toxicity and developmental abnormalities. To assess the ecological health risk of PFAS in Pennsylvania stream surface water, we conducted a comprehensive analysis that included both measured and predicted estimates. The potential combined exposure effects of 14 individual PFAS to aquatic biota were estimated using the sum of exposure-activity ratios ( Σ EARs) in 280 streams. Additionally, machine learning techniques were utilized to predict potential PFAS exposure effects in unmonitored stream reaches, considering factors such as land use, climate, and geology. Leveraging a tailored convolutional neural network (CNN), a validation accuracy of 78% was achieved, directly outperforming traditional methods that were also used, such as logistic regression and gradient boosting (accuracies of ~65%). Feature importance analysis highlighted key variables that contributed to the CNN’s predictive power. The most influential features highlighted the complex interplay of anthropogenic and environmental factors contributing to PFAS contamination in surface waters. Industrial and urban land cover, rainfall intensity, underlying geology, agricultural factors, and their interactions emerged as key determinants. These findings may help to inform biotic sampling strategies, water quality monitoring efforts, and policy decisions aimed to mitigate the ecological impacts of PFAS in surface waters.

Pennsylvania↗

Using a time-of-travel sampling approach to quantify per- and polyfluoroalkyl substances (PFAS) stream loading and source inputs in a mixed-source, urban catchment

Understanding per- and polyfluoroalkyl substances (PFAS) mass distribution in surface and groundwater systems can support source prioritization, load reduction, and water management. Thirteen sites within an urban catchment were sampled utilizing a time-of-travel sampling approach to minimize the influence of subdaily fluctuations in mass from PFAS point sources and to quantify PFAS and ancillary chemical loads from various PFAS sources. A larger increase in perfluoroalkyl sulfonate (PFSA) loads (8 to 11 μg/s, up to 618%) than in perfluoroalkyl carboxylate (PFCA) loads (no change to 3.4 μg/s, up to 122%) was observed at sites below tributaries influenced by military bases with known groundwater discharge. Point discharges from two sewage treatment plants (STPs) resulted in increases in PFCA and PFSA loads that were similar (6 and 10 μg/s respectively) below the first STP and greater for PFCA compared to PFSA loads (23 and 13 μg/s respectively) below the second STP. Overall, percent increases in total PFAS load ranged from 20 to 277% for military base inputs and 44 to 77% for STP inputs. A focus catchment that represents only 14% (76.9 km 2 ) of the drainage area at the most downstream site (544 km 2 ) accounted for about 70% of PFSA and 40% of PFCA loads observed at the most downstream site. Results show that by using a time-of-travel sampling approach in mixed, urban settings with several PFAS sources, it is possible to quantify stream loads from individual PFAS sources, thereby improving source attribution and providing actionable data for water-resource managers.

ACS ES&T Water↗

Integrated science for the study of microplastics in the environment—A strategic science vision for the U.S. Geological Survey

Executive Summary Evidence of the widespread occurrence of microplastics throughout our environment and exposure to humans and other organisms over the past decade has led to questions about the possibility of health hazards and mitigation of exposures. This document discusses nanoplastics as well as microplastics (referred to solely as microplastics); the microplastics have a range from 1 micrometer to 5 millimeters (1 μm–5 mm) in length, whereas the nanoplastics are less than 1 μm in length (sidebar ES1). A myriad of environmental exposure pathways with microplastics to humans and wildlife, including ingestion, inhalation, and bodily absorption, are likely to exist. A growing body of evidence has documented bioaccumulation of microplastics in tissues and organs of humans and wildlife, benthic community effects, and potential nutritional and reproductive effects in some wildlife species. Understanding if or when environmental exposures pose a health risk is complicated by the diversity of microplastic sizes, morphologies, polymer types, and chemicals added during manufacturing or sorbed from the environment; ongoing challenges in analytical methods used to detect, quantify, and characterize microplastics and associated chemicals in our ecosystems; and the fact that ecotoxicological studies regarding microplastics are still in their infancy. Therefore, the study of environmental exposures and potential related health hazards of microplastics to the public and wildlife is a One Health (sidebar ES2) research topic that necessitates integrated science approaches. A better understanding of the sources, pathways, fate, and biological effects of microplastics has become a priority of the Federal Government, State governments, Tribes, stakeholders, and the public. Examples of Federal and State microplasticfocused legislation and programs to prioritize microplastic research and reduction include the Federal Microbead-Free Waters Act of 2015, California Senate Bills 1422 and 1263 (2018), the U.S. Environmental Protection Agency (EPA) Trash Free Waters Program, the National Institute of Standards and Technology’s Microplastic and Nanoplastic Metrology project, and Minnesota’s microplastic project. With its unique expertise and capabilities, the U.S. Geological Survey (USGS) is well positioned to help fill some of the most important microplastic science gaps. This strategic science vision document for microplastics identifies current (2023) microplastic science gaps and prioritizes research relevant to the mission, expertise, and capabilities of the USGS. It is intended for USGS scientists and stakeholders to use as a starting point for planning, prioritizing, and designing collaborative environmental microplastic science. Many of the microplastic science gaps and priorities are scalable, from local to national, and thus, can be made commensurate with available funding and evolving analytical and field tools, laboratory capacity, and stakeholder needs. Current (2023) or future research by academia and other Federal or State agencies, and Tribes may be aimed at some of the same microplastic science gaps identified in this document. Therefore, this document can be used as an information resource to maximize strengths and capabilities and minimize redundancy in communication and collaboration.

Circular↗

Per- and polyfluorinated alkyl substances (PFAS) in Pennsylvania surface waters: A statewide assessment, associated sources, and land-use relations

The objectives of this study are to identify per- and polyfluoroalkyl substances (PFAS) in Pennsylvania surface waters, corresponding associations with potential sources of PFAS contamination (PSOC) and other parameters, and compare raw surface water concentrations to human and ecological benchmarks. Surface water samples from 161 streams were collected in September 2019 and were analyzed for 33 target PFAS and water chemistry. Land use and physical attributes in upstream catchments and geospatial counts of PSOC in local catchments are summarized. The hydrologic yield of the sum of 33 PFAS (∑PFAS) for each stream was computed by normalizing each site's load by the drainage area of the upstream catchment. Utilizing conditional inference tree analysis, the percentage of development (>7.58 %) was identified as a primary driver of the ∑PFAS hydrologic yields. When percentage of development was removed from analysis, ∑PFAS yields were closely related to surface water chemistry associated with landscape alteration (e.g., development or agricultural cropland), such as concentrations of total nitrogen, chloride, and ammonia, but also to count of water pollution control facilities (agricultural, industrial, stormwater, and/or municipal waste pollution abatement facilities). In oil and gas development regions, ∑PFAS yields were associated with combined sewage outfalls. Sites surrounded by ≥2 electronic manufacturing facilities had elevated ∑PFAS yields (median = 241 ng/s/km 2 ). Study results are critical to guide future research, regulatory policy, best practices that will mitigate PFAS contamination, and the communication of human health and ecological risks associated with PFAS exposure from surface waters.

Pennsylvania↗

Corrigendum: Associations between cyanobacteria and indices of secondary production in the western basin of Lake Erie

In the last year, we became aware that data used in our above-referenced manuscript from 2018 published in Limnology and Oceanography contained significant errors. In the 2018 manuscript, we found that indices of secondary production were negatively correlated to indices of cyanobacterial abundance and toxicity. Unfortunately, one of our indices of cyanobacterial abundance (biovolume) and our measurement of toxicity (microcystin concentration) were inaccurate in the data we used in the 2018 manuscript. Upon discovering these errors, we immediately began correcting the repositories where these data were available. Having corrected those data repositories, we are now reporting on the re-analysis of the data using the methods previously described in the 2018 manuscript. Although the relationships are slightly different, our interpretation is that the conclusions of the 2018 manuscript are still valid using the corrected data. The data errors we experienced were traced to a spreadsheet that was used to share data among the research team, which had errors caused by mistakes in copy-pasting formulas instead of data and ‘inadvertent’ edits that were saved by the spreadsheet's auto-save function. We apologize to the scientific community for these errors.

Lake Erie↗

Characterization of ambient groundwater quality within a statewide, fixed-station monitoring network in Pennsylvania, 2015–19

Pennsylvania leads the Nation in the number of individuals that use groundwater for private domestic water supply; more than 3 million rural and suburban Pennsylvania residents rely on private domestic supplies for drinking water. These supplies are not regulated nor routinely monitored; thus relevant groundwater-quality information is not widely available. The U.S. Geological Survey (USGS), in cooperation with the Pennsylvania Department of Environmental Protection (PaDEP) Safe Drinking Water Bureau, established a statewide, fixed-station ambient groundwater quality network in 2015. The goals for the Pennsylvania Groundwater Monitoring Network (GWMN) include characterizing ambient groundwater quality conditions in rural areas of the State and documenting potential changes in conditions over time. Seventeen wells were selected for monitoring at 6-month intervals beginning in 2015. Since then, several wells have been added to the GWMN, bringing the total number of wells sampled in the fall of 2019 to 28. Routinely monitored constituents included physical characteristics and chemical concentrations in filtered and unfiltered samples (major and trace elements, nutrients, and organic compounds). Samples for volatile organic compounds (VOCs), radionuclides, and dissolved hydrocarbon gases were collected during the first sampling event at each well. To offer insights on the quality of groundwater used for domestic supply in Pennsylvania, summary statistics for the 221 GWMN samples collected during 2015–19 are compared to U.S. Environmental Protection Agency (EPA) drinking-water standards, which are applicable to public water supplies. Results show that samples across the GWMN generally meet drinking-water standards for inorganic and organic constituents; however, a percentage of samples had concentrations that exceeded maximum contaminant level (MCL) thresholds for nitrate (3 percent) and secondary maximum contaminant level (SMCL) thresholds for iron (32 percent), manganese (36 percent), and aluminum (5 percent). Radon-222 activities, which were sampled only during the initial visit to a well, exceeded the lower proposed drinking water standard of 300 picocuries per liter (pCi/L) in 64 percent of wells in the GWMN; additionally, 7 percent of wells exceeded the higher proposed standard of 4,000 pCi/L. There were no exceedances for VOCs, but one well had a tribromomethane detection. Three wells had detectable concentrations of methane, with one sample exceeding the Pennsylvania action level of 7 milligrams per liter (mg/L). The pH and dissolved oxygen concentrations varied widely across the GWMN and were correlated with dissolved metal concentrations and other chemical characteristics of groundwater samples. Considering all samples collected for the study, the pH ranged from 4.2 to 8.3; 42 percent of pH values were either above or below the SMCL range of 6.5–8.5. The highest pH values resulted from contamination of loose grout used in the construction of one well and decreased to levels consistent with other wells in the vicinity after repeated sampling rounds. Dissolved oxygen (DO), which ranged from 0 to 13.9 mg/L, influences the mobility and prevalence of constituents with variable oxidation state, including iron, manganese, and nitrogen species. Samples with acidic pH (less than 6.5) and (or) low DO had the highest concentrations of manganese and iron, whereas those with neutral to alkaline pH values had the highest concentrations of calcium, magnesium, sodium, and other major ions. Analysis of major ions indicates that calcium/bicarbonate water types are the most common, with a few characterized as calcium/chloride or sodium/chloride, and most others as mixed water types including calcium-magnesium/bicarbonate, sodium-magnesium/bicarbonate, and sodium/bicarbonate-chloride. Nonparametric statistical methods were used to evaluate the data for spatial and temporal trends. A principal components analysis (PCA) model developed with ranked data values for the entire network resulted in three components, (1) dissolved solids, (2) redox, and (3) sodium-chloride, which explained 74.5 percent of variance in the dataset. On the basis of individual contributions to the PCA, certain wells were identified through hierarchical cluster analysis that shared relevant water-quality characteristics. The spatial distribution of sampling locations and the temporal trends of constituent concentrations indicate that hydrogeologic setting and topographic position as defined in the PCA model are important factors affecting the spatial and temporal patterns of groundwater quality in the GWMN.

Pennsylvania↗

Effects of legacy sediment removal and effects on nutrients and sediment in Big Spring Run, Lancaster County, Pennsylvania, 2009–15

Big Spring Run is a 1.68-square mile watershed underlain by mostly carbonate rock in a mixed land-use setting (part agricultural and part developed) in Lancaster County, Pennsylvania. Big Spring Run is a subwatershed of Mill Creek, a tributary to the Conestoga River. These watersheds are known contributors of nutrient and sediment loads to the Chesapeake Bay and several stream reaches are on the Pennsylvania impaired waters list. Big Spring Run is listed as impaired and was selected by the Pennsylvania Department of Environmental Protection to evaluate a novel best management practice to restore natural aquatic ecosystems by removing legacy sediment. The study was designed to quantify sediment and nutrient contributions in pre- and postrestoration periods (water years 2009–11 and 2012–15, respectively) using an intensive monitoring approach at three surface-water sites within the watershed. Instrumentation at each site continuously measured (15-minute intervals) streamflow, water temperature, and turbidity. Water-quality samples were collected routinely (generally monthly and during selected storms); sampling frequency varied by site and constituent at the three monitoring sites. Effects of legacy sediment removal and restoration on nutrient concentrations varied in surface water samples depending on the form (particulate, dissolved, organic, inorganic). For example, total phosphorus concentrations at the downstream site decreased from a median of 0.19 milligram per liter (mg/L) to 0.04 mg/L, pre- and postrestoration periods, respectively. Concentrations of orthophosphate, the dissolved form of phosphorus, were not significantly different pre- to postrestoration at the downstream site. Similarly, nitrate concentrations, the dominant form of nitrogen in Big Spring Run surface-water samples (92.3 percent of total nitrogen) were not significantly different in the pre- compared to the postrestoration periods. Legacy sediment removal and restoration had significant effects on suspended-sediment concentrations and loads. Median suspended-sediment concentrations at the downstream site decreased from 556 mg/L prerestoration to 74 mg/L postrestoration even though streamflow hydrographs during the two periods were similar. In the postrestoration period, the mean annual suspended-sediment load conveyed to the restoration area from the upstream sites was 839 tons, whereas mean annual suspended-sediment load at the downstream site was reduced to 242 tons. Streamflow during storms transports a large proportion of the suspended-sediment load; there were a total of 320 storms over the study period. In Big Spring Run, a single storm event can transport more than 25 percent of the annual suspended-sediment load. The greatest single-storm contribution to suspended-sediment load was 38 percent in water year 2015 at the downstream site. Although streamflow magnitudes during storms varied greatly over the study period, median streamflow was 17.5 cubic feet per second and median duration was about 3 hours and 24 minutes. Results observed for this study using the newly proposed best management practice were compared with other best management practices intended to reduce sediment. For example, during a previous study, statistically significant reductions in suspended-sediment concentration were observed when streambank fencing was implemented in an adjacent watershed; however, suspended-sediment reductions were an order of magnitude less than the reductions observed in the current study. Median suspended-sediment concentration at the downstream site was reduced by 482 mg/L in the current study compared to only 30 to 46 mg/L as a result of streambank fencing.

Pennsylvania↗

Spatial distribution of nutrients, chloride, and suspended sediment concentrations and loads determined by using different sampling methods in a cross section of the Trenton Channel of the Detroit River, Michigan, November 2014–November 2015

The Detroit River separates the United States and Canada as it flows from Lake St. Clair to Lake Erie. The Trenton Channel is a 13-kilometer-long branch of the Detroit River that flows to the west of Grosse Ile before rejoining the Detroit River near its mouth, just before the Detroit River flows into Lake Erie. The U.S. Environmental Protection Agency has listed both the Trenton Channel and Detroit River as Areas of Concern because of a list of Beneficial Use Impairments such as interrupted drinking-water services, loss of aquatic life, and reduced recreational use. Phosphorus loading from tributaries such as the Trenton Channel is one of the primary drivers of eutrophication in Lake Erie. The complex flow patterns and variable distribution of chemical constituents in the Trenton Channel make it difficult to accurately characterize the concentrations and loads of nutrients and other constituents conveyed through the channel to Lake Erie. In order to better understand the Trenton Channel’s contributions of nutrients (total phosphorus, orthophosphate, total nitrogen, and ammonia), chloride, and suspended sediment to Lake Erie and evaluate differences in results obtained by using different sample methodologies, the U.S. Geological Survey, in cooperation with the U.S. Environmental Protection Agency and Environment Canada, completed 12 sampling campaigns on the Trenton Channel in Detroit, Michigan, from November 2014 through November 2015. Acoustic Doppler current profiler (ADCP) techniques were used to characterize the distribution of velocity components within a cross section corresponding to a transect of the Trenton Channel at U.S. Geological Survey station 041686401 Trenton Channel of Detroit River at Grosse Ile, Mich. Three methods of collecting water-quality data at the same transect of the Trenton Channel were used: multiple-vertical depth-integrated (MVDI), fixed-point, and discrete samples. Horizontal and vertical variations in concentrations of nutrients, chloride, and suspended sediment were analyzed from discrete samples to better understand distributions of these constituents throughout the channel. Constituent loads were calculated by using individual sample concentrations and ADCP measurements for discharge made on the same day that the water-quality samples were collected. Constituent loads calculated from MVDI and fixed-point sampling methods were compared. The relation between MVDI and fixed-point samples helped quantify the differences between the sampling methods. Linear regression equations depicting the relation between concentrations measured by using MVDI and fixed-point samples were prepared. ADCP data indicates that velocities throughout the sampled transect remain uniform except for one location around 200 meters from the west bank of the channel. Secondary flow vectors suggest the presence of counter-rotating helical flow cells, and these helical flow cells could affect the mixing of constituents in transport by preventing cross-channel mixing. Flow discharges throughout the sampling campaign showed small variations, although lower flow rates were observed in the early winter months than in the summer months. Discrete sampling methods results displayed both heterogeneity throughout the channel horizontally, representing limited horizontal mixing in the channel, and displayed homogeneity throughout vertical transects, indicating mixing vertically. Comparisons between MVDI and fixed-point methods found consistently higher concentrations were measured in MVDI samples compared to concentrations measured in fixed-point samples. To correct for this bias between MVDI and fixed-point sample results, simple linear-regression equations were developed for all major constituents to help estimate constituent concentrations from fixed-point samples equivalent to those measured by using MVDI sampling techniques. Instantaneous constituent loads were developed by using velocity and discharge data obtained from ADCPs and constituent concentrations obtained from MVDI and fixed-point samples.

Michigan↗

Temporal trends in water-quality constituent concentrations and annual loads of chemical constituents in Michigan watersheds, 1998–2013

In 1998, the Michigan Department of Environmental Quality and the U.S. Geological Survey began the Water Chemistry Monitoring Program for select streams in the State of Michigan. Objectives of this program were to provide assistance with (1) statewide water-quality assessments, (2) the National Pollutant Discharge Elimination System permitting process, and (3) water-resource management decisions. As part of this program, water-quality data collected from 1998 to 2013 were analyzed to identify potential trends for select constituents that were sampled. Sixteen water-quality constituents were analyzed at 32 stations throughout Michigan. Trend analysis on the various water-quality data was done using either the uncensored Seasonal Kendall test or through Tobit regression. In total, 79 trends were detected in the constituents analyzed for 32 river stations sampled for the study period—53 downward trends and 26 upward trends were detected. The most prevalent trend detected throughout the State was for ammonia, with 11 downward trends and 1 upward trend estimated. In addition to trends, constituent loads were estimated for 31 stations from 2002 to 2013 for stations that were sampled 12 times per year. Loads were computed using the Autobeale load computation program, which used the Beale ratio estimator approach to estimate an annual load. Constituent loads were the largest in large watershed streams with the highest annual flows such as the Saginaw and Grand Rivers. Likewise, constituent loads were the smallest in smaller tributaries that were sampled as part of this program such as the Boardman and Thunder Bay Rivers.

Michigan↗

Groundwater flux and nutrient loading in the northeast section of Bear Lake, Muskegon County, Michigan, 2015

Bear Lake in North Muskegon, Michigan, is listed as part of the Muskegon Lake area of concern as designated by the U.S. Environmental Protection Agency. This area of concern was designated as a result of eutrophication and beneficial use impairments. On the northeast end of Bear Lake, two man-made retention ponds (Willbrandt Pond East and Willbrandt Pond West), formerly used for celery farming, may contribute nutrients to Bear Lake. Willbrandt Ponds (East and West) were previously muck fields that were actively used for celery farming from the early 1900s until 2002. The restoration and reconnection of the Willbrandt Ponds into Bear Lake prompted concerns of groundwater nutrient loading into Bear Lake. Studies done by the State of Michigan and Grand Valley State University revised initial internal phosphorus load estimates and indicated an imbalance in the phosphorus budget in Bear Lake. From June through November 2015, the U.S. Geological Survey (USGS) did an investigative study to quantify the load of nutrients from shallow groundwater around the Willbrandt Ponds in an effort to update the phosphorus budget to Bear Lake. Seven sampling locations were established, including five shallow groundwater wells and two surface-water sites, in the Willbrandt pond study area and Bear Lake. A total of 12 nutrient samples and discrete water-level measurements were collected from each site from June through November 2015. Continuous water-level data were recorded for both surface-water monitoring locations for the entire sampling period. Water-level data indicated that Willbrandt Pond West had the highest average water-level elevation of all sites monitored, which indicated the general direction of flux is from Willbrandt Pond West to Bear Lake. Nutrient and chloride loading from Willbrandt Pond West to Bear Lake was calculated using two distinct methods: Dupuit and direct seepage methods. Shallow groundwater loading calculations were determined by using groundwater levels to first determine a flux of shallow groundwater, then nutrient concentrations to determine a load. It was determined that Willbrandt Pond East and Willbrandt Pond West contributed between 2 to 4 percent of the total annual phosphorus load to Bear Lake by way of shallow groundwater flow. Annual loads calculated for other constituents include orthophosphate (40–100 pounds per year [lb P/yr]), total nitrogen (200–830 lb/yr), chloride (12,700–32,100 lb/yr), and ammonia (130–670 lb N/yr). Study results indicated that mean groundwater and surface-water nutrient concentrations calculated in this study were higher than reported Michigan statewide values. The data collected in this study allow understanding of groundwater nutrient loading into Bear Lake in an effort to help inform future restoration and management decisions.

Michigan↗

Associations between cyanobacteria and indices of secondary production in the western basin of Lake Erie

Large lakes provide a variety of ecological services to surrounding cities and communities. Many of these services are supported by ecological processes that are threatened by the increasing prevalence of cyanobacterial blooms which occur as aquatic ecosystems experience cultural eutrophication. Over the past 10 yr, Lake Erie experienced cyanobacterial blooms of increasing severity and frequency, which have resulted in impaired drinking water for the surrounding communities. Cyanobacterial blooms may impact ecological processes that support other services, but many of these impacts have not been documented. Secondary production (production of primary consumers) is an important process that supports economically important higher trophic levels. Cyanobacterial blooms may influence secondary production because cyanobacteria are a poor‐quality food resource and cyanotoxins may be harmful to consumers. Over 3 yr at 34 sites across the western basin of Lake Erie, we measured three indices of secondary production that focus on the dominant bivalve taxa: (1) growth of a native unionid mussel, (2) the size of young‐of‐year dreissenid mussels, and (3) the mass of colonizing animals on a Hester‐Dendy sampler. Associations between these indices and cyanobacterial data were estimated to assess whether cyanobacteria are associated with variation in secondary production in the western basin of Lake Erie. The results suggest cyanobacterial abundance alone is only weakly associated with secondary production, but that cyanotoxins have a larger effect on secondary production. Given recurring late‐summer cyanobacterial blooms, this impact on secondary production has the potential to undermine Lake Erie's ability to sustain important ecosystem services.

Lake Erie↗

Water quality and hydrology of Silver Lake, Oceana County, Michigan, with emphasis on lake response to nutrient loading

Executive Summary Silver Lake is a 672-acre inland lake located in Oceana County, Michigan, and is a major tourist destination due to its proximity to Lake Michigan and the surrounding outdoor recreational opportunities. In recent years, Silver Lake exhibited patterns of high phosphorus concentrations, elevated chlorophyll a concentrations, and nuisance algal blooms. The U.S. Geological Survey (USGS), in cooperation with the Silver Lake Improvement Board and in collaboration with the Annis Water Resources Institute (AWRI) of Grand Valley State University, designed a study to assess the hydrologic and nutrient inputs to Silver Lake in order to identify the events and conditions that affect the nutrient chemistry and production of algal blooms in the lake. This information can inform water-resource managers in developing various management strategies to prevent or reduce the occurrence of future algal blooms. USGS and AWRI scientists collected data from November 2012 to December 2014 to provide information for future management decisions for Silver Lake. Silver Lake can be classified as a polymictic lake and has a residence time of approximately 223 days. Based on the mean lake Secchi depth, total phosphorus, and total nitrogen concentrations, Silver Lake is classified as a eutrophic lake. In-situ bioassay results indicate that algal growth in Silver Lake is colimited by both nitrogen and phosphorus. The nutrient budget for Silver Lake was calculated using the BATHTUB model based on 2 years of water-quality data collection. The BATHTUB model, developed by the U.S. Army Corps of Engineers, treats the lake as a well-mixed system with multiple inputs and outlets for both water and dissolved constituents, such as nutrients. Based on results of the BATHTUB model, which were conditioned on observed concentrations and flows, the mean annual input of phosphorus to Silver Lake was approximately 1,342 pounds (lb); the mean annual input of nitrogen to Silver Lake was approximately 51,998 lb. The major measured sources of phosphorus loading to Silver Lake were groundwater and Hunter Creek, whereas the major measured sources of nitrogen to Silver Lake were Hunter Creek, groundwater, and atmospheric deposition. The largest loading of phosphorus and nitrogen to Silver Lake occurred during the spring. Minimal phosphorus deposition (if any) occurred in the lakebed sediment; however, of the nitrogen that entered Silver Lake, approximately 42.2 percent was deposited in the lakebed sediment as simulated by the BATHTUB model. In addition to measured sources, a septic load model was used to estimate the likely range of septic contribution to groundwater and adjacent surface waters. The likely septic loading scenario estimates that septic systems contribute 47.8 percent of the phosphorus to groundwater and 22.3 percent of phosphorus to Hunter Creek. These results indicate that septic systems are a major source of phosphorus loading to Silver Lake. The likely septic loading scenario indicated that septic systems account for 0.95 percent of the nitrogen load to Hunter Creek and 1.1 percent of the contribution of nitrogen to groundwater. The BATHTUB model was used to estimate future nutrient loading and eutrophication scenarios based on water-quality data collected from Silver Lake, groundwater, major tributaries, and atmospheric deposition. A separate septic load model was used to estimate the septic contribution to groundwater or directly to surface water, and the nutrient load estimates were modeled using the BATHTUB model to determine subsequent water-quality changes to Silver Lake. BATHTUB model scenarios based on measured data: The first BATHTUB scenario evaluated the condition of Silver Lake and the change to lake water quality (trophic status) as a result of changes in nutrient loading from different sources. Based on BATHTUB model simulations, if groundwater loading of phosphorus and nitrogen only were decreased by 75 percent, and all of the other nutrient inputs stayed the same, the future condition of Silver Lake would most likely remain highly mesotrophic to eutrophic (the current [2014] condition of Silver Lake). If nutrient loading continued to increase in groundwater, the lake would continue to remain eutrophic with more frequent algal blooms. If nutrient loading from Hunter Creek only decreased by 50–75 percent, and all of the other nutrient inputs stayed the same as the baseline dataset, Silver Lake would remain eutrophic to highly mesotrophic. By reducing the input of manageable nutrient sources (Hunter Creek, groundwater, and lawn runoff) by 75 percent, the BATHTUB model simulation indicates that Silver Lake would be classified as mesotrophic, which is indicative of improved water quality, water clarity, and reduced algal bloom frequency. Simulations also were run using the BATHTUB model to evaluate the number of days Silver Lake could experience algal blooms (algal blooms are defined as modeled chlorophyll a in excess of 10 micrograms per liter [µg/L]) as a result of an increase/decrease in phosphorus and nitrogen loading from groundwater, Hunter Creek, and (or) a combination of sources. If the phosphorus and nitrogen loading from Hunter Creek is decreased (and all other sources are not altered), Silver Lake will continue to experience algal blooms, but less frequently than what is currently experienced. The same scenario holds true if the nutrient loading from groundwater is decreased. Another scenario was simulated using a combination of sources, which includes increases and decreases in phosphorus and nitrogen loading from sources that are the most likely to be managed, and includes groundwater (as a result of conversion of household septic to sewers), Hunter Creek (conversion of household septic to sewers), and lawn runoff. Results of the BATHTUB model indicated that a 50-percent reduction of phosphorus and nitrogen from these sources would result in a considerable decrease in algal bloom frequency (from 231 to 132 days) and severity, and a 75-percent reduction would greatly reduce algal bloom occurrence on Silver Lake (from 231 to 57 days). BATHTUB model scenarios based on septic load model: A scenario also was conducted using the BATHTUB model to simulate the conversion of septic to sewer and included a low, high, and medium (likely) scenario of nutrient loading to Silver Lake. Simulations of the BATHTUB model indicated that, under the likely scenario, the conversion of all onsite septic treatment to sewers would result in an overall change in lake trophic status from eutrophic to mesotrophic, thereby reducing the frequency of algal blooms and algal bloom intensity on Silver Lake (chlorophyll a >10 µg/L, from 231 to 184 days per year, or chlorophyll a >20 µg/L, from 80 to 49 days per year).

Michigan↗

Contamination with bacterial zoonotic pathogen genes in U.S. streams influenced by varying types of animal agriculture

Animal waste, stream water, and streambed sediment from 19 small (< 32 km 2 ) watersheds in 12 U.S. states having either no major animal agriculture (control, n = 4), or predominantly beef ( n = 4), dairy ( n = 3), swine ( n = 5), or poultry ( n = 3) were tested for: 1) cholesterol, coprostanol, estrone, and fecal indicator bacteria (FIB) concentrations, and 2) shiga-toxin producing and enterotoxigenic Escherichia coli , Salmonella , Campylobacter , and pathogenic and vancomycin-resistant enterococci by polymerase chain reaction (PCR) on enrichments, and/or direct quantitative PCR. Pathogen genes were most frequently detected in dairy wastes, followed by beef, swine and poultry wastes in that order; there was only one detection of an animal-source-specific pathogen gene ( stx1 ) in any water or sediment sample in any control watershed. Post-rainfall pathogen gene numbers in stream water were significantly correlated with FIB, cholesterol and coprostanol concentrations, and were most highly correlated in dairy watershed samples collected from 3 different states. Although collected across multiple states and ecoregions, animal-waste gene profiles were distinctive via discriminant analysis. Stream water gene profiles could also be discriminated by the watershed animal type. Although pathogen genes were not abundant in stream water or streambed samples, PCR on enrichments indicated that many genes were from viable organisms, including several (shiga-toxin producing or enterotoxigenic E. coli , Salmonella , vancomycin-resistant enterococci) that could potentially affect either human or animal health. Pathogen gene numbers and types in stream water samples were influenced most by animal type, by local factors such as whether animals had stream access, and by the amount of local rainfall, and not by studied watershed soil or physical characteristics. Our results indicated that stream water in small agricultural U.S. watersheds was susceptible to pathogen gene inputs under typical agricultural practices and environmental conditions. Pathogen gene profiles may offer the potential to address both source of, and risks associated with, fecal pollution.

Arizona, Indiana, Iowa, Kentucky, Maryland, Michig↗

Detection of hepatitis E virus and other livestock-related pathogens in Iowa streams

Manure application is a source of pathogens to the environment. Through overland runoff and tile drainage, zoonotic pathogens can contaminate surface water and streambed sediment and could affect both wildlife and human health. This study examined the environmental occurrence of gene markers for livestock-related bacterial, protozoan, and viral pathogens and antibiotic resistance in surface waters within the South Fork Iowa River basin before and after periods of swine manure application on agricultural land. Increased concentrations of indicator bacteria after manure application exceeding Iowa's state bacteria water quality standards suggest that swine manure contributes to diminished water quality and may pose a risk to human health. Additionally, the occurrence of HEV and numerous bacterial pathogen genes for Escherichia coli , Enterococcus spp., Salmonella sp., and Staphylococcus aureus in both manure samples and in corresponding surface water following periods of manure application suggests a potential role for swine in the spreading of zoonotic pathogens to the surrounding environment. During this study, several zoonotic pathogens were detected including Shiga-toxin producing E. coli , Campylobacter jejuni , pathogenic enterococci, and S. aureus ; all of which can pose mild to serious health risks to swine, humans, and other wildlife. This research provides the foundational understanding required for future assessment of the risk to environmental health from livestock-related zoonotic pathogen exposures in this region. This information could also be important for maintaining swine herd biosecurity and protecting the health of wildlife near swine facilities.

Iowa↗

Pre/post-closure assessment of groundwater pharmaceutical fate in a wastewater‑facility-impacted stream reach

Pharmaceutical contamination of contiguous groundwater is a substantial concern in wastewater-impacted streams, due to ubiquity in effluent, high aqueous mobility, designed bioactivity, and to effluent-driven hydraulic gradients. Wastewater treatment facility (WWTF) closures are rare environmental remediation events; offering unique insights into contaminant persistence, long-term wastewater impacts, and ecosystem recovery processes. The USGS conducted a combined pre/post-closure groundwater assessment adjacent to an effluent-impacted reach of Fourmile Creek, Ankeny, Iowa, USA. Higher surface-water concentrations, consistent surface-water to groundwater concentration gradients, and sustained groundwater detections tens of meters from the stream bank demonstrated the importance of WWTF effluent as the source of groundwater pharmaceuticals as well as the persistence of these contaminants under effluent-driven, pre-closure conditions. The number of analytes (110 total) detected in surface water decreased from 69 prior to closure down to 8 in the first post-closure sampling event approximately 30 d later, with a corresponding 2 order of magnitude decrease in the cumulative concentration of detected analytes. Post-closure cumulative concentrations of detected analytes were approximately 5 times higher in proximal groundwater than in surface water. About 40% of the 21 contaminants detected in a downstream groundwater transect immediately before WWTF closure exhibited rapid attenuation with estimated half-lives on the order of a few days; however, a comparable number exhibited no consistent attenuation during the year-long post-closure assessment. The results demonstrate the potential for effluent-impacted shallow groundwater systems to accumulate pharmaceutical contaminants and serve as long-term residual sources, further increasing the risk of adverse ecological effects in groundwater and the near-stream ecosystem.

Iowa↗

Understanding the hydrologic impacts of wastewater treatment plant discharge to shallow groundwater: Before and after plant shutdown

Effluent-impacted surface water has the potential to transport not only water, but wastewater-derived contaminants to shallow groundwater systems. To better understand the effects of effluent discharge on in-stream and near-stream hydrologic conditions in wastewater-impacted systems, water-level changes were monitored in hyporheic-zone and shallow-groundwater piezometers in a reach of Fourmile Creek adjacent to and downstream of the Ankeny (Iowa, USA) wastewater treatment plant (WWTP). Water-level changes were monitored from approximately 1.5 months before to 0.5 months after WWTP closure. Diurnal patterns in WWTP discharge were closely mirrored in stream and shallow-groundwater levels immediately upstream and up to 3 km downstream of the outfall, indicating that such discharge was the primary control on water levels before shutdown. The hydrologic response to WWTP shutdown was immediately observed throughout the study reach, verifying the far-reaching hydraulic connectivity and associated contaminant transport risk. The movement of WWTP effluent into alluvial aquifers has implications for potential WWTP-derived contamination of shallow groundwater far removed from the WWTP outfall.

Iowa↗

Genes indicative of zoonotic and swine pathogens are persistent in stream water and sediment following a swine manure spill

Manure spills to streams are relatively frequent, but no studies have characterized stream contamination with zoonotic and veterinary pathogens, or fecal chemicals, following a spill. We tested stream water and sediment over 25 days and downstream for 7.6 km for: fecal indicator bacteria (FIB); the fecal indicator chemicals cholesterol and coprostanol; 20 genes for zoonotic and swine-specific bacterial pathogens by presence/absence polymerase chain reaction (PCR) for viable cells; one swine-specific Escherichia coli toxin gene ( STII ) by quantitative PCR (qPCR); and nine human and animal viruses by qPCR, or reverse-transcriptase qPCR. Twelve days post-spill, and 4.2 km downstream, water concentrations of FIB, cholesterol, and coprostanol were 1-2 orders of magnitude greater than those detected before, or above, the spill, and genes indicating viable zoonotic or swine-infectious Escherichia coli , were detected in water or sediment. STII increased from undetectable before, or above the spill, to 10 5 copies/100 mL water 12 days post-spill. Thirteen of 14 water (8/9 sediment) samples had viable STII -carrying cells post-spill. Eighteen days post-spill porcine adenovirus and teschovirus were detected 5.6 km downstream. Sediment FIB concentrations (per gram wet weight) were greater than in water, and sediment was a continuous reservoir of genes and chemicals post-spill. Constituent concentrations were much lower, and detections less frequent, in a runoff event (200 days post-spill) following manure application, although the swine-associated STII and stx 2e genes were detected. Manure spills are an underappreciated pathway for livestock-derived contaminants to enter streams, with persistent environmental outcomes, and the potential for human and veterinary health consequences.

Iowa↗

Occurrence and distribution of fecal indicator bacteria and gene markers of pathogenic bacteria in Great Lakes tributaries, March-October 2011

From March through October 2011, the U.S. Geological Survey (USGS), conducted a study to determine the frequency of occurrence of pathogen gene markers and densities of fecal indicator bacteria (FIB) in 22 tributaries to the Great Lakes. This project was funded as part of the Great Lakes Restoration Initiative (GLRI) and included sampling at 22 locations throughout 6 states that border the Great Lakes. A total of 177 environmental samples were collected at USGS streamgaging stations during both normal-flow and high-flow conditions and were analyzed by the Michigan Bacteriological Research Laboratory at the USGS Water Science Center in Lansing, Michigan. Water samples were analyzed for the presence of FIB concentrations (FIB; fecal coliform bacteria, Escherichia coli [ E. coli ], and enterococci) by using membrane filtration and serial dilution methods. The resulting enrichments from standard culturing of the samples were then analyzed by using polymerase chain reaction (PCR) to determine the occurrence of pathogen gene markers for Shigella species, Campylobacter jejuni and coli , Salmonella species, and pathogenic E. coli , including Shiga toxin-producing E. coli (STEC).

Great Lakes↗