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John W. Allran

Publications and source records attributed to John W. Allran.

2 recordsLinked to original sources

Effects of pore‐water ammonia on in situ survival and growth of juvenile mussels (;Lampsilis cardium); in the St. Croix Riverway, Wisconsin, USA

We conducted a series of in situ tests to evaluate the effects of pore‐water ammonia on juvenile Lampsilis cardium in the St. Croix River (WI, USA). Threats to this river and its associated unionid fauna have accelerated in recent years because of its proximity to Minneapolis–St. Paul, Minnesota, USA. In 2000, caged juveniles were exposed to sediments and overlying water at 12 sites for 10 d. Survival and growth of juveniles was significantly different between sediment (mean, 47%) and water column (mean, 86%) exposures; however, these effects were unrelated to pore‐water ammonia. During 2001, juveniles were exposed to sediments for 4, 10, and 28 d. Pore‐water ammonia concentrations ranged from 0.3 to 62.0 μg NH 3 ‐N/L in sediments and from 0.5 to 140.8 μg NH 3 ‐N/L within exposure chambers. Survival (mean, 45, 28, and 41% at 4, 10, and 28 d, respectively) and growth (range, 3–45 μm/d) of juveniles were highly variable and generally unrelated to ammonia concentrations. Although laboratory studies have shown unionids to be quite sensitive to ammonia, further research is needed to identify the route(s) of ammonia exposure in unionids and to understand the factors that contribute to the spatial variability of ammonia in rivers.

Minnesota, Wisconsin↗

Effects of ammonia on juvenile unionid mussels ( Lampsilis cardium ) in laboratory sediment toxicity tests

Ammonia is a relatively toxic compound generated in water and sediments by heterotrophic bacteria and accumulates in sediments and pore water. Recent data suggest that unionid mussels are sensitive to un-ionized ammonia (NH3) relative to other organisms. Existing sediment exposure systems are not suitable for ammonia toxicity studies with juvenile unionids; thus, we modified a system to expose juveniles to ammonia that was continuously infused into sediments. This system maintained consistent concentrations of ammonia in pore water up to 10 d. Juvenile Lampsilis cardium mussels were exposed to NH 3 in pore water in replicate 96-h and 10-d sediment toxicity tests. The 96-h median lethal concentrations (LC50s) were 127 and 165 μg NH 3 -N/L, and the 10-d LC50s were 93 and 140 μg NH 3 -N/L. The median effective concentrations (EC50s) (based on the proportion affected, including dead and inactive mussels) were 73 and 119 μg NH 3 -N/L in the 96-h tests and 71 and 99 μg NH 3 -N/L in the 10-d tests. Growth rate was substantially reduced at concentrations between 31 and 76 μg NH 3 -N/L. The lethality results (when expressed as total ammonia) are about one-half the acute national water quality criteria for total ammonia, suggesting that existing criteria may not protect juvenile unionids.

Environmental Toxicology and Chemistry↗