Geology topics

James A. Luoma

Publications and source records attributed to James A. Luoma.

31 records · Page 2Linked to original sources

USGS2016

Development of targeted delivery techniques for Zequanox®

The effects of water temperature and concentration on the physical characteristics of Zequanox®, a dead-cell spray-dried powder formulation of Pseudomonas fluorescens (strain CL145A) used for controlling invasive dreissenid mussels (zebra mussel, Dreissena polymorpha, and quagga mussel, Dreissena bugensis), were investigated to determine optimal temperature-specific concentrations and delivery techniques for use during open-water subsurface Zequanox applications. Temperature-controlled laboratory tests evaluated viscosity, settling, stratification, and buoyancy of various concentrations of Zequanox suspension in water to select an optimal target viscosity for Zequanox applications. A two-step linear regression procedure was used to create a temperature-specific Zequanox prediction model from the viscosity data. The prediction model and subsurface application techniques were validated by conducting three independent outdoor pond trials at temperatures of ~9, 14, and 20°C. During these outdoor trials, subsurface applications of Zequanox at concentrations predicted by the model were performed and water samples were collected at varying depths and analyzed via spectroscopy to determine Zequanox concentration and dispersion. Although the predicted Zequanox concentrations and delivery techniques used resulted in successfully maintaining lethal Zequanox concentrations in the bottom 7.5 cm of the water column for the duration of the exposure, a revised prediction model is also provided for more accurately selecting temperature-specific Zequanox concentrations.

Report↗

USGS2016

Efficacy of spray –Dried Pseudomonas fluorescens, strain CL145A (Zequanox®), for controlling Zebra Mussels ( Dreissena polymorpha ) within Lake Minnetonka, MN enclosures

The efficacy of whole water column and subsurface applications of the biopesticide Zequanox®, a commercially prepared spray-dried powder formulation of Pseudomonas fluorescens (strain CL145A), were evaluated for controlling zebra mussels (Dreissena polymorpha) within 27-m2 enclosures in Lake Minnetonka (Deephaven, Minnesota). Five treatments consisting of (1) two whole water column Zequanox applications, (2) two subsurface Zequanox applications, and (3) an untreated control were completed on each of three independent treatment days during September 2014. The two types of samplers used in the study were (1) type 1 samplers, which were custom built multi-plate samplers (wood, perforated aluminum, and tile substrates) that were placed into Robinson’s Bay in June of 2013 to allow for natural colonization by zebra mussels, and (2) type 2 samplers, which consisted of zebra mussels adhering to perforated aluminum trays that were placed into mesh containment bags. One day prior to treatment, three individual samplers of each type were distributed to test enclosures and exposed to a randomly assigned treatment. Sampling to determine the zebra mussel biomass adhering to type 1 samplers and the survival assessments for zebra mussels contained in type 2 samplers were completed ~40 days after exposure. The zebra mussel biomass adhering to type 1 samplers and the survival of zebra mussels contained in type 2 samplers were significantly less in groups treated with the highest Zequanox concentrations and in groups that received whole water column applications than comparable groups treated with lower Zequanox concentrations and subsurface applications. However, standardization of biomass and survival results to the amount of Zequanox applied showed that the lower concentrations and subsurface applications were more cost efficient, with respect to product used, at reducing zebra mussel biomass and for inducing zebra mussel mortality. Although the subsurface application methods and lower treatment concentrations were more cost efficient, biological significance and management goals should be evaluated prior to selecting the application method. Development and refinement of additional application techniques may improve the utility of the subsurface Zequanox applications.

Report↗

USGS2016

Effects of spray-dried Pseudomonas fluorescens, strain CL145A (Zequanox®) on reproduction and early development of the fathead minnow (Pimephales promelas).

The biopesticide, Zequanox®, is registered for dreissenid mussel control in open water systems. Previous toxicity trials with nontarget organisms, including young-of-the year of several fish species and invertebrates, demonstrated selectivity of Zequanox for dreissenids. However, data are lacking on its safety to reproductive and early life stages of fish. The present study evaluated the effects of Zequanox on spawning and early life stages of the fathead minnow, Pimephales promelas, at the maximum approved concentration (100 mg Zequanox active ingredient /L) and exposure duration (8 h) for open water application. The results showed no significant effect of Zequanox on survival, condition, or cumulative egg deposition (21 d) in adult fathead minnow. Eggs (<24-h old) exposed to Zequanox developed to the eyed-stage at a similar rate to that of unexposed eggs. Additionally, Zequanox did not have a significant effect on survival and growth (90 d) of newly hatched fry (<24-h old). The results indicate that Zequanox treatment will not affect survival, spawning, and early life development of fathead minnows when applied at the recommended treatment regime.

Report↗

USGS2015

Efficacy of Pseudomonas fluorescens (Pf-CL145A) spray dried powder for controlling zebra mussels adhering to test substrates

A mobile bioassay trailer was used to assess the efficacy of Pseudomonas fluorescens ( Pf -CL145A) spray dried powder (SDP) formulation for controlling zebra mussels ( Dreissena polymorpha ) from two midwestern lakes: Lake Carlos (Alexandria, Minnesota) and Shawano Lake (Shawano, Wisconsin). The effects of SDP exposure concentration and exposure duration on zebra mussel survival were evaluated along with the evaluation of a benthic injection application technique to reduce the amount of SDP required to induce zebra mortality. Groups of zebra mussels were collected from each lake and allowed to adhere to test substrates for at least 15 days before exposure to SDP. Two independent trials were completed at each lake: (1) a whole water column (WWC) application trial was used to evaluate the effects of SDP exposure concentration and exposure duration on zebra mussel survival; and (2) a benthic injection (BI) application trial in which the SDP was injected into the test tanks to determine the efficacy of a benthic injection application technique to reduce the amount of SDP required to induced zebra mussel mortality. Three exposure durations (6, 9, and 12 hours) were evaluated in the WWC trials and a 12-hour exposure duration was evaluated in the BI trials. All trials contained zebra mussels which were removed at the completion of each exposure duration, consolidated into wire mesh cages, and held in the lake for approximately 30 days before being assessed for survival. For all trials, treatment was assigned to each test tank according to a randomized block design ( n = 3 test tanks per treatment). The treatment groups included (1) an untreated control group, (2) a group that received an application of 50 milligrams of SDP per liter (mg SDP/L), and (3) a group that received an application of 100 mg SDP/L. During the BI trials, SDP was administered to achieve the desired exposure concentration in the bottom 50 percent (175 L) of the test tank. All exposure concentrations are reported as active ingredient. Approximately 30 days after exposure, zebra mussels were sorted into live and dead, and enumerated. Mean survival of zebra mussels in control treatments exceeded 95 percent. Mean survival of zebra mussels in the Lake Carlos WWC SDP-treated groups ranged from 0.5 to 2.1 percent and when compared at the same exposure duration, no difference was detected in survival between the 50 and 100 milligrams per liter (mg/L) treatment groups. Similarly, mean survival of zebra mussels in the Shawano Lake WWC SDP-treated groups ranged from 2.0 to 12.6 percent and when compared at the same exposure duration, no difference was detected in survival between the 50- and 100-mg/L treatment groups. Mean survival of zebra mussels in the Lake Carlos BI trial SDP-treated groups did not differ ( p = 0.93) and was 18.1 and 18.0 percent in the 50- and 100-mg/L treatment groups, respectively. Mean survival of zebra mussels in the Shawano Lake BI trial SDP-treated groups differed ( p < 0.01) and was 2.9 and 0.9 percent in the 50- and 100-mg/L treatment groups, respectively. Survival of zebra mussels assigned to the SDP-treated groups in the Lake Carlos WWC trial (12-hour exposure duration) differed from the survival of zebra mussels assigned to the SDP-treated groups in the Lake Carlos BI trial; however, after modification of the BI application technique, no difference ( p = 0.22) was detected between the survival of zebra mussel in the Shawano Lake WWC (12-hour exposure duration) and BI trials.

Minnesota, Wisconsin↗

USGS2015

Efficacy of Pseudomonas fluorescens strain CL145A spray dried powder for controlling zebra mussels adhering to native unionid mussels within field enclosures

The efficacy of a commercially prepared spray dried powder (SDP) formulation of Pseudomonas fluorescens (strain CL145A) was evaluated for removing zebra mussels ( Dreissena polymorpha ) adhering to a population of unionid mussels in Lake Darling (Alexandria, Minnesota). Two groups of unionid mussels were used in the study. Unionid mussels were collected near the test area, weighed, photographed, individually tagged, and randomly allocated to one of nine test enclosures in equal proportions and then divided into two groups. The first group of unionid mussels (Group 1, n = 5 per test enclosure) were indiscriminately selected from each test enclosure and used to estimate the number of zebra mussels adhering to unionid mussels prior to exposure. The second group of unionid mussels (Group 2, n = 22 per test enclosure) were used to evaluate the efficacy of SDP for removal of adhering zebra mussels. Both Group 1 and Group 2 mussels were used to evaluate the effects of SDP exposure on unionid mussel survival. Treatment was assigned to each test enclosure by using a randomized block design. The three treatment groups were tested in triplicate and included an untreated control group and groups that received a single application of 50 or 100 milligrams per liter (mg/L) of SDP based on active ingredient. All treatment concentrations are reported as active ingredient of SDP. Test enclosures were removed at the 8-hour exposure termination. Both Group 1 and Group 2 mussels remained in their assigned exposure location during the postexposure holding period. The number of zebra mussels adhering to Group 2 mussels (live and dead) was assessed 18 to 20 days postexposure in addition to assessing the survival of Group 1 and Group 2 unionid mussels. SDP, administered as a single treatment, significantly ( p < 0.01) reduced the number of adhering zebra mussels when compared to the untreated controls. The number of zebra mussels adhering to unionid mussels (Group 2) was reduced 53 percent in the 50-mg/L treatment group and 68 percent in the 100-mg/L treatment group. The number of adhering zebra mussels did not differ ( p = 0.79) between the 50- and 100-mg/L treatment groups after exposure. When standardized to the amount of SDP applied per square meter, each gram (g) of SDP applied in the 50-mg/L treatment reduced the number of adhering zebra mussel 59.8 percent more than the 100-mg/L treatment group. Group 1 mussel survival did not differ between treatment groups ( p > 0.05); however, a difference was detected ( p < 0.01) in the survival of Group 2 mussels. The survival of Group 2 mussels did not differ ( p > 0.23) between control and treated groups. A difference in Group 2 mussel survival was detected ( p = 0.03; odds ratio [OR] = 0.290) between the 50- and 100-mg/L treatment groups (that is, the survival was highest in the 50-mg/L treatment group and lowest in the 100-mg/L treatment group), however, the biological significance of the difference is indeterminate.

Minnesota↗

USGS2015

Safety of spray-dried powder formulated Pseudomonas fluorescens strain CL145A exposure to subadult/adult unionid mussels during simulated open-water treatments

The exposure effects of a commercially prepared spray dried powder (SDP) formulation of Pseudomonas fluorescens (strain CL145A) on the survival of seven species of unionid mussels endemic to the Great Lakes and Mississippi River basins was evaluated in this study. The study exposures were completed within replicated 350-liter test tanks contained within a mobile bioassay laboratory sited on the shores of the Black River near La Crosse, Wisconsin. The test tanks were supplied with flowing, filtered river water which was interrupted during the exposure period. Two groups of seven species of mussels were used in equal proportions in the study. The first group was exposed to SDP for 8 hours, and the second group was exposed to SDP for 24 hours. Individually tagged mussels were randomly allocated to test tanks until all test tanks contained 8 to 10 mussels of each species (dependent upon the number available for testing). The experimental unit for the trial was the individual test tank, and treatment group was assigned using a randomized block design. The treatment groups for each exposure duration consisted of (1) an untreated control group, (2) a group that received an application of 50 milligrams SDP per liter (mg SDP/L), and (3) a group that received an application of 100 mg SDP/L. All mussel species and both exposure duration groups were exposed concurrently (that is, one-half of the mussels were removed after 8 hours of SDP exposure and the remaining mussels were removed after 24 hours of SDP exposure). All treatment concentrations are reported as active ingredient. After exposure, the mussels were consolidated into wire mesh cages and placed in the Black River for a 27-28 day postexposure period, after which time survival of mussels was assessed. Of the 1,170 mussels tested in the study, 3 were confirmed dead and 5 were not recovered and treated as mortalities in the analysis. The effect and interactions of species, SDP exposure concentration, and SDP exposure duration were analyzed and did not affect mussel survival (p > 0.98). The results from this study indicate that SDP exposure at the maximum approved open-water concentration of 100 mg/L for up to 3 times the maximum approved open-water exposure duration of 8 hours (in other words for 24 hours of exposure) is unlikely to reduce survival of subadult or adult mussels.

Wisconsin↗

USGS2015

Exposure-related effects of Pseudomonas fluorescens ( Pf -CL145A) on juvenile unionid mussels

The exposure-related effects of a commercially prepared spray-dried powder (SDP) or freeze-dried powder (FDP) formulation of Pseudomonas fluorescens (strain CL145A) on the survival of seven species of newly metamorphosed (<72 hours old) freshwater unionid mussels was evaluated. Forty unionid mussels of each species were randomly distributed to test chambers and each species independently exposed for 24 hours to a static dose of either SDP (four species: Lampsilis cardium , Lampsilis siliquoidea , Lampsilis higginsii , and Ligumia recta ) or FDP (three species: Obovaria olivaria , Actinonaias ligamentina , and Megalonaias nervosa ). Each test chamber was assigned to one of six treatment groups ( n = four chambers per group) by using a randomized block design. The six treatment groups included (1) an untreated control group, (2) groups that received applications with nominal target active ingredient (AI) concentrations of 50, 100, 200, and 300 milligrams per liter (mg/L), and (3) a group that received an application with a nominal target AI concentration of 300 mg/L of heat-deactivated test article (300 HD). After a 24-hour exposure period, water inflow to the test chambers was restored, and the unionid mussels were maintained for an additional 7 days before they were assessed for survival. Mean survival of four unionid mussels species exposed to SDP varied among species and treatment groups when compared to the untreated control groups. The results indicate that exposure to SDP-formulated P. fluorescens up to the maximum label concentration (100 mg/L AI) and up to three times the maximum label exposure duration (8 hours) is not likely to affect the survival of L. siliquoidea and L. higginsii . Low mean survival in the L. recta control group (25.0 percent) indicates that results for L. recta should be interpreted with caution. Mean survival of the L. cardium was significantly lower in all treated groups (14.4 to 40.6 percent) compared to the control group (68.8 percent). These results indicate that further investigation on the impact of SDP-formulated P. fluorescens on L. recta and L. cardium is warranted. Mean survival of three unionid mussels species exposed to FDP was not significantly different in the 50-, 100-, and 200-mg/L AI treatment groups and the 300 mg/L heat-deactivated treatment groups when compared to the control groups. Mean survival of O. olivaria and M. nervosa was significantly lower in the 300-mg/L AI treated groups (38.1 and 48.1 percent, respectively) compared to the control groups (71.9 and 88.1 percent, respectively). The results indicate that exposure to FDP-formulated P. fluorescens up to the maximum label concentration (100 mg/L AI) and up to three times the maximum label exposure duration (8 hours) is not likely to affect the survival of O. olivaria , A. ligamentina , and M. nervosa .

Open-File Report↗

USGS2015

Exposure-related effects of formulated Pseudomonas fluorescens strain CL145A to glochidia from seven unionid mussel species

The study was completed to evaluate the exposure-related effects of a biopesticide for dreissenid mussel ( Dreissena polymorpha , zebra mussel and Dreissena rostriformis bugensis , quagga mussel) control on glochidia from unionid mussels endemic to the Great Lakes and Upper Mississippi River Basins. The commercially prepared biopesticide was either a spray-dried powder (SDP) or freeze-dried powder (FDP) formulation of Pseudomonas fluorescens , strain CL145A. Glochidia of the unionid mussel species Lampsilis cardium , Lampsilis siliquoidea , Lampsilis higginsii , Ligumia recta , Obovaria olivaria , and Actinonaias ligamentina were exposed to SDP-formulated P. fluorescens and Lampsilis cardium and Megalonaias nervosa were exposed to FDP-formulated P. fluorescens . All exposures were static, 24 hours in duration, and included six treatment groups. The treatment groups included (1) an untreated control, (2) a positive control which received a nominal target active ingredient (AI) concentration of 300 milligrams per liter (mg/L) of heat-deactivated test article, and (3) treatments that received nominal target AI concentrations of 50, 100, 200, and 300 mg/L of test article. All treatment concentrations are reported based on active ingredient. Glochidia viability was reduced in two of the six species exposed to 50 mg/L SDP and in four of the six species exposed to 100 mg/L SDP when compared to untreated control groups at 6, 12, and 24 hours. Regardless of sample time, concentrations of 200 and 300 mg/L of SDP and 300 mg/L of heat-deactivated SDP (positive control) substantially reduced glochidia viability in all species except, L . higginsii . Glochidia viability was only reduced for L. cardium exposed to FDP at concentrations ≥ 200 mg/L. After 24 hours of FDP exposure, differences in glochidia viability were only detected in M . nervosa that were exposed to 300 mg/L of heat-deactivated SDP. However, given the low viability in the control group, the results for M . nervosa should be interpreted with caution.

Open-File Report↗

USGS2015

Exposure-related effects of Pseudomonas fluorescens , strain CL145A, on coldwater, coolwater, and warmwater fish

The exposure-related effects of a commercially prepared spray-dried powder (SDP) formulation of Pseudomonas fluorescens , strain CL145A, were evaluated on coldwater, coolwater, and warmwater fish endemic to the Great Lakes and Upper Mississippi River Basins. Nine species of young-of-the-year fish were exposed to SDP for 24 hours by using continuous-flow, serial-dilution exposure systems at temperatures of 12 degrees Celsius (°C; 2 species; Oncorhynchus mykiss [rainbow trout] and Salvelinus fontinalis [brook trout]), 17 °C (3 species; Perca flavescens [yellow perch], Sander vitreus [walleye], and Acipenser fulvescens [lake sturgeon]), or 22 °C (4 species; Micropterus salmoides [largemouth bass], Micropterus dolomieu [smallmouth bass], Lepomis macrochirus [bluegill sunfish], and Ictalurus punctatus [channel catfish]). Treatments, which were nominal target concentrations of SDP (as active ingredient) of 50, 100, 200, and 300 milligrams per liter (mg/L), were continuously applied for 24 hours by the addition of a test article stock solution into the main water inflow of each exposure system's dilution box. The SDP-treated water was then serially diluted through a series of dilution cells before delivery to the test chambers. The exposure concentrations measured were 61.5 to 81.4 percent of the target concentration. After exposure, fish were monitored for 22 days to assess exposure-related latent effects. Analyses of test animal condition factors and survival revealed that a 24-hour continuous dose of SDP affected all species. Calculated concentrations of SDP that would be lethal to 50 percent of the test animals (LC 50 ) for the coldwater species were 19.2 and 104.6 mg/L for rainbow and brook trout, respectively. The LC 50 's for the coolwater species were 185.4, 176.9 and 8.9 mg/L for yellow perch, walleye, and lake sturgeon, respectively. The LC 50 's for the warmwater species were 173.6, 139.4, and 63.1 for the largemouth bass, smallmouth bass, and channel catfish, respectively. A reliable LC 50 for bluegill sunfish could not be calculated because mortality in the SDP-treated groups did not exceed 20 percent. Further investigations to evaluate the SDP-exposure related effects on freshwater fish at the maximum approved open-water label concentration and exposure duration (100 mg/L for 8 hours) and using the expected lentic application technique (static application) are warranted. The variation in tolerance to P. fluorescens , strain CL145A, exposure observed in this study indicates that fish species community composition should be considered before SDP is applied in open-water environments.

Open-File Report↗

USGS2012

Assessing consumption of bioactive micro-particles by filter-feeding Asian carp

Silver carp Hypophthalmichthys molitrix (SVC) and bighead carp H. nobilis (BHC) have impacted waters in the US since their escape. Current chemical controls for aquatic nuisance species are non-selective. Development of a bioactive micro-particle that exploits filter-feeding habits of SVC or BHC could result in a new control tool. It is not fully understood if SVC or BHC will consume bioactive micro-particles. Two discrete trials were performed to: 1) evaluate if SVC and BHC consume the candidate micro-particle formulation; 2) determine what size they consume; 3) establish methods to evaluate consumption of filter-feeders for future experiments. Both SVC and BHC were exposed to small (50-100 μm) and large (150-200 μm) micro-particles in two 24-h trials. Particles in water were counted electronically and manually (microscopy). Particles on gill rakers were counted manually and intestinal tracts inspected for the presence of micro-particles. In Trial 1, both manual and electronic count data confirmed reductions of both size particles; SVC appeared to remove more small particles than large; more BHC consumed particles; SVC had fewer overall particles in their gill rakers than BHC. In Trial 2, electronic counts confirmed reductions of both size particles; both SVC and BHC consumed particles, yet more SVC consumed micro-particles compared to BHC. Of the fish that ate micro-particles, SVC consumed more than BHC. It is recommended to use multiple metrics to assess consumption of candidate micro-particles by filter-feeders when attempting to distinguish differential particle consumption. This study has implications for developing micro-particles for species-specific delivery of bioactive controls to help fisheries, provides some methods for further experiments with bioactive micro-particles, and may also have applications in aquaculture.

Journal of Aquaculture Research & Development↗

USGS2010

Effects of water hardness on size and hatching success of silver carp eggs

Eggs of silver carp Hypophthalmichthys molitrix absorb water after release from the female, causing them to become turgid and to increase substantially in size. The volume of water that diffuses within an egg is most likely determined by (1) the difference in ionic concentration between the egg and the water that surrounds it and (2) the elasticity of the egg membrane. Prior observations suggest that silver carp eggs may swell and burst in soft waters. If water hardness affects silver carp reproductive success in nonnative ecosystems, this abiotic factor could limit silver carp distribution or abundance. In this study, we tested the effect of water hardness on silver carp egg enlargement and hatching success. Groups of newly fertilized silver carp eggs were placed in water at one of five nominal water hardness levels (50, 100, 150, 200, or 250 mg/L as CaCO 3 ) for 1 h to harden (absorb water after fertilization). Egg groups were then placed in separate incubation vessels housed in two recirculation systems that were supplied with either soft (50 mg/L as CaCO 3 ) or hard (250 mg/L as CaCO 3 ) water to evaluate hatching success. Tests were terminated within 24 h after viable eggs had hatched. Eggs that were initially placed in 50-mg/L water to harden were larger (i.e., swelled more) and had a greater probability of hatch than eggs hardened in other water hardness levels. Unlike the effect of water hardness during egg hardening, the water hardness during incubation appeared to have no effect on egg hatching success. Our research suggests that water hardness may not be a limiting factor in the reproduction, recruitment, and range expansion of silver carp in North America.

North American Journal of Fisheries Management↗

USGS2000

Influences of dietary uptake and reactive sulfides on metal bioavailability from aquatic sediments

Understanding how animals are exposed to the large repository of metal pollutants in aquatic sediments is complicated and is important in regulatory decisions. Experiments with four types of invertebrates showed that feeding behavior and dietary uptake control bioaccumulation of cadmium, silver, nickel, and zinc. Metal concentrations in animal tissue correlated with metal concentrations extracted from sediments, but not with metal in porewater, across a range of reactive sulfide concentrations, from 0.5 to 30 micromoles per gram. These results contradict the notion that metal bioavailability in sediments is controlled by geochemical equilibration of metals between porewater and reactive sulfides, a proposed basis for regulatory criteria for metals.

Science↗

USGS1998

Acute toxicity and accumulation of the piscicide 3-trifluoromethyl-4-nitrophenol (TFM) in freshwater mussels (Bivalvia: Unionidae)

We compared the acute toxicity and initial accumulation of the piscicide TFM (3-trifluoromethyl-4-nitrophenol) in the freshwater unionacean mussels, Obliquaria reflexa and Fusconaia flava . Acute 48 h toxicity tests were conducted to determine the LC 50 values for each species. The initial uptake clearances of TFM were measured by exposing the mussels to [ 14 C]-TFM and counting the radioactivity in four organ tissues (foot, gill, mantle and viscera) over 48 h. TFM was about 2-fold more toxic to O. reflexa (LC 50 1.80 mg l -1 ) than to F. flava (LC 50 3.81 mg L -1 ) and the difference was not explained by accumulation patterns. The initial uptake clearance rates (0–6 h) for the whole body were similar between the species (11.2 ml g -1 h -2 in O. reflexa and 9.5 ml g -1 h -1 in F. flava ). The accumulation of TFM residues among the organ tissues was also similar between species. The uptake clearance rates (ml g -1 h -1 ) of TFM equivalents were generally highest in the gill, but not significantly different than other organ tissues. The normalized concentration of TFM residues (μg per g whole body) was highest in the viscera. The toxicity data suggest that the mortality of both species would be minimal from sea lamprey control treatments with TFM. However, the behavioural effects and accumulation rates also indicate that mussels do not effectively avoid TFM exposure by valve closure.

Ecotoxicology↗