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J.W. Duris

Publications and source records attributed to J.W. Duris.

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Comparison of streamflow and water-quality data collection techniques for the Saginaw River, Michigan

In 2009, the Michigan Department of Environmental Quality and the U.S. Geological Survey developed a plan to compare the effect of various streamgaging and water-quality collection techniques on streamflow and stream water-quality data for the Saginaw River, Michigan. The Saginaw River is the primary contributor of surface runoff to Saginaw Bay, Lake Huron, draining approximately 70 percent of the Saginaw Bay watershed. The U.S. Environmental Protection Agency has listed the Saginaw Bay system as an "Area of Concern" due to many factors, including excessive sediment and nutrient concentrations in the water. Current efforts to estimate loading of sediment and nutrients to Saginaw Bay utilize water-quality samples collected using a surface-grab technique and flow data that are uncertain during specific conditions. Comparisons of current flow and water-quality sampling techniques to alternative techniques were assessed between April 2009 and September 2009 at two locations in the Saginaw River. Streamflow estimated using acoustic Doppler current profiling technology was compared to a traditional stage-discharge technique. Complex conditions resulting from the influence of Saginaw Bay on the Saginaw River were able to be captured using the acoustic technology, while the traditional stage-discharge technique failed to quantify these effects. Water-quality samples were collected at two locations and on eight different dates, utilizing both surface-grab and depth-integrating multiple-vertical techniques. Sixteen paired samples were collected and analyzed for suspended sediment, turbidity, total phosphorus, total nitrogen, orthophosphate, nitrite, nitrate, and ammonia. Results indicate that concentrations of constituents associated with suspended material, such as suspended sediment, turbidity, and total phosphorus, are underestimated when samples are collected using the surface-grab technique. The median magnitude of the relative percent difference in concentration based on sampling technique was 37 percent for suspended sediment, 26 percent for turbidity, and 9.7 percent for total phosphorus samples collected at both. Acoustic techniques were also used to assist in the determination of the effectiveness of using acoustic-backscatter information for estimating the suspended-sediment concentration of the river water. Backscatter data was collected by use of an acoustic Doppler current profiler, and a Van Dorn manual sampler was simultaneously used to collect discrete water samples at 10 depths (3.5, 7.5, 11, 14, 15.5, 17.5, 19.5, 20.5, 22, and 24.5 ft below the water surface) along two vertical profiles near the center of the Saginaw River near Bay City. The Van Dorn samples were analyzed for suspended-sediment concentrations, and these data were then used to develop a relationship between acoustic-backscatter data. Acoustic-backscatter data was strongly correlated to sediment concentrations and, by using a linear regression, was able to explain 89 percent of the variability. Although this regression technique showed promise for using acoustic backscatter to estimate suspended-sediment concentration, attempts to compare suspended-sediment concentrations to the acoustic signal-to-noise ratio estimates, recorded at the fixed acoustic streamflow-gaging station near Bay City (04157061), resulted in a poor correlation.

Michigan

Bacterial and archaeal phylogenetic diversity of a cold sulfur-rich spring on the shoreline of Lake Erie, Michigan

Studies of sulfidic springs have provided new insights into microbial metabolism, groundwater biogeochemistry, and geologic processes. We investigated Great Sulphur Spring on the western shore of Lake Erie and evaluated the phylogenetic affiliations of 189 bacterial and 77 archaeal 16S rRNA gene sequences from three habitats: the spring origin (11-m depth), bacterial-algal mats on the spring pond surface, and whitish filamentous materials from the spring drain. Water from the spring origin water was cold, pH 6.3, and anoxic (H 2 , 5.4 nM; CH 4 , 2.70 ??M) with concentrations of S 2- (0.03 mM), SO 4 2- (14.8 mM), Ca 2+ (15.7 mM), and HCO 3 - (4.1 mM) similar to those in groundwater from the local aquifer. No archaeal and few bacterial sequences were >95% similar to sequences of cultivated organisms. Bacterial sequences were largely affiliated with sulfur-metabolizing or chemolithotrophic taxa in Beta-, Gamma-, Delta-, and Epsilonproteobacteria. Epsilonproteobacteria sequences similar to those obtained from other sulfidic environments and a new clade of Cyanobacteria sequences were particularly abundant (16% and 40%, respectively) in the spring origin clone library. Crenarchaeota sequences associated with archaeal-bacterial consortia in whitish filaments at a German sulfidic spring were detected only in a similar habitat at Great Sulphur Spring. This study expands the geographic distribution of many uncultured Archaea and Bacteria sequences to the Laurentian Great Lakes, indicates possible roles for epsilonproteobacteria in local aquifer chemistry and karst formation, documents new oscillatorioid Cyanobacteria lineages, and shows that uncultured, cold-adapted Crenarchaeota sequences may comprise a significant part of the microbial community of some sulfidic environments. Copyright ?? 2009, American Society for Microbiology. All Rights Reserved.

Applied and Environmental Microbiology

Gene and antigen markers of Shiga-toxin producing E. coli from Michigan and Indiana river water: Occurrence and relation to recreational water quality criteria

The relation of bacterial pathogen occurrence to fecal indicator bacteria (FIB) concentrations used for recreational water quality criteria (RWQC) is poorly understood. This study determined the occurrence of Shiga-toxin producing Escherichia coli (STEC) markers and their relation to FIB concentrations in Michigan and Indiana river water. Using 67 fecal coliform (FC) bacteria cultures from 41 river sites in multiple watersheds, we evaluated the occurrence of five STEC markers: the Escherichia coli (EC) O157 antigen and gene, and the STEC virulence genes eae A, stx 1, and stx 2. Simple isolations from selected FC cultures yielded viable EC O157. By both antigen and gene assays, EC O157 was detected in a greater proportion of samples exceeding rather than meeting FC RWQC ( P < 0.05), but was unrelated to EC and enterococci RWQC. The occurrence of all other STEC markers was unrelated to any FIB RWQC. The eaeA , stx2, and stx1 genes were found in 93.3, 13.3, and in 46.7% of samples meeting FC RWQC and in 91.7, 0.0, and 37.5% of samples meeting the EC RWQC. Although not statistically significant, the percentage of samples positive for each STEC marker except stx1 was lower in samples that met, as opposed to exceeded, FIB RWQC. Viable STEC were common members of the FC communities in river water throughout southern Michigan and northern Indiana, regardless of FIB RWQC. Our study indicates that further information on the occurrence of pathogens in recreational waters, and research on alternative indicators of their occurrence, may help inform water-resource management and public health decision-making.

Journal of Environmental Quality

The microbial community structure in petroleum-contaminated sediments corresponds to geophysical signatures

The interdependence between geoelectrical signatures at underground petroleum plumes and the structures of subsurface microbial communities was investigated. For sediments contaminated with light non-aqueousphase liquids, anomalous high conductivity values have been observed. Vertical changes in the geoelectrical properties of the sediments were concomitant with significant changes in the microbial community structures as determined by the construction and evaluation of 16S rRNA gene libraries. DNA sequencing of clones from four 16S rRNA gene libraries from different depths of a contaminated field site and two libraries from an uncontaminated background site revealed spatial heterogeneity in the microbial community structures. Correspondence analysis showed that the presence of distinct microbial populations, including the various hydrocarbon-degrading, syntrophic, sulfate-reducing, and dissimilatory-iron-reducing populations, was a contributing factor to the elevated geoelectrical measurements. Thus, through their growth and metabolic activities, microbial populations that have adapted to the use of petroleum as a carbon source can strongly influence their geophysical surroundings. Since changes in the geophysical properties of contaminated sediments parallel changes in the microbial community compositions, it is suggested that geoelectrical measurements can be a cost-efficient tool to guide microbiological sampling for microbial ecology studies during the monitoring of natural or engineered bioremediation processes. Copyright ?? 2007, American Society for Microbiology. All Rights Reserved.

Applied and Environmental Microbiology