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H. M. Stuckey

Publications and source records attributed to H. M. Stuckey.

At least 19 recordsLinked to original sources
USGS2006

Enteric redmouth bacterium: Comparison of isolates from different geographic areas

Characteristics of 18 strains of Yersinia ruckeri the causative agent of enteric redmouth disease, from a variety of hosts and a wide geographic range were compared. Except for variations in colonial morphology and in citrate, sorbitol, and trehalose reactions, the strains were morphologically and biochemically homogeneous. Sixteen strains reacted with antiserum against the first serotype reported, and one strain reacted with antiserum against a more recently reported serotype. One strain represented a third serotype. Evidence presented indicated that extension of the geographic range may not be entirely a result of the transport of carrier fish.

Journal of Fish Diseases↗
USGS1987

Studies on vertical transmission of Aeromonas salmonicida

In four attempts to effect vertical transmission of Aeromonas salmonicida, the cause of furunculosis, transmission did not occur. Progeny of both naturally infected and artificially infected brood stock of rainbow trout (Salmo gairdneri) and brook trout (Salvelinus fontinalis) did not develop furunculosis during a 10‐12‐month posthatching observation period.

Progressive Fish-Culturist↗
USGS1976

Comparative susceptibility of Atlantic salmon (Salmo salar) to the enteric redmouth bacterium and Aeromonas salmonicida

The bacterium causing enteric redmouth (ERM) and Aeromonas salmonicida were found to be equally pathogenic for fingerling Atlantic salmon ( Salmo salar ). Injection of 5 × 10 5 cells of ERM or A. salmonicida killed all salmon within 96 h. After a 30 min exposure to water-borne cells of the two test bacteria about one-half of the test salmon died within 14 days. Both ERM and A. salmonicida were transmitted horizontally. Results indicate that efforts should be made to prevent introduction of ERM into watersheds where Atlantic salmon occur.

Journal of Wildlife Diseases↗
USGS1975

Fluorescent antibody identification and detection of the Corynebacterium causing kidney disease of salmonids

An indirect fluorescent antibody technique (FAT) has been developed for serological identification, diagnosis of kidney disease (KD), and detection of subclinical infections of KD. Rabbit or goat antikidney disease serum and appropriately labeled immunoglobulin was found to be specific for the KD bacterium. The technique was superior to examination of gram-stained smears for detection of small numbers of KD bacteria. The test requires approximately 2 h.

Journal of the Fisheries Research Board of Canada↗
USGS1974

Corynebacterial kidney disease of salmonids: Growth and serological studies on the causative bacterium

Comparative growth studies of 10 isolates of the Corynebacterium of salmonid kidney disease were quantified on four media. Greatest cell yield was obtained from Mueller-Hinton medium with 0.1% L-cysteine hydrochloride. Serological tests, in which rabbit antisera used against typical bacteria strains showed all to be antigenically homologous, provided a reliable method of identification.

Applied Microbiology↗
USGS1974

In vitro and in vivo efficacy of potentiated sulfonamide in control of furunculosis in salmonids

In vitro and in vivo efficacy of a potentiated sulffonamide, Ro5–0037, for inhibition of Aeromonas salmonicida , causative agent of fish furunculosis was determined. The components of the potentiated sulfonamide, sulfadimethoxine, and ormetoprim were tested alone and in combination. In vitro results showed that both sulfonamide-resistant and sensitive strains of A . salmonicida had greater sensitivity to Ro5–0037 than to either of its components. Also, sulfonamide-sensitive strains developed resistance to ormetoprim and sulfadimethoxine more rapidly than to Ro5–0037. Efficacy studies with small numbers of artificially infected trout revealed that Ro5–0037 effectively controlled furunculosis at 50 mg/kg of fish/day, one fourth the usual sulfonamide dosage level. Extensive field trials also showed the 50 mg/kg level of Ro5–0037 gave effective control of hatchery outbreaks of furunculosis. As found in the in vitro studies, all in vivo trials indicated the combination of ormetoprim and sulfadimethoxine was more efficient in inhibiting A . salmonicida than either component alone.

Journal of the Fisheries Research Board of Canada↗
USGS1974

Serological diagnosis of corynebacterial kidney disease of salmonids

An immunodiffusion test is described which provides a rapid, sensitive, and definitive diagnosis of corynebacterial kidney disease of salmonid fishes. Specific precipitin lines are formed within 24 h when rabbit antiserum is reacted with kidney disease cell suspensions or internal tissue homogenates containing the specific bacterium. No precipitin lines are formed with tissue homogenates from healthy fish or from those injected with other fish pathogenic bacteria.

Journal of the Fisheries Research Board of Canada↗