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H. G. Ketola

Publications and source records attributed to H. G. Ketola.

At least 19 recordsLinked to original sources
USGS2011

Efficacy and toxicity of iodine disinfection of Atlantic salmon eggs

Recent interest in the restoration of Atlantic salmon Salmo salar in the Great Lakes has given rise to new culture techniques and management programs designed to reduce pathogen transmission while stabilizing and enhancing wild populations. We examined the toxicity of iodine to Atlantic salmon eggs and its effectiveness as a disinfectant against bacteria on egg surfaces. We spawned and fertilized eight gravid Atlantic salmon from Cayuga Lake, New York, and exposed their eggs to 10 concentrations of iodine (5, 10, 50, 75, 100, 500, 750, 1,000, 5,000, and 7,500 mg/L) for 30 min during water hardening. An additional subsample of unfertilized eggs was also exposed to some of the same concentrations of iodine (5, 10, 50, 75, and 100 mg/L) to determine the efficiency of disinfection. Viable eggs were only obtained from four females. Survival of eggs to the eyed stage and hatch tended to be reduced at iodine concentrations of 50 and 75 mg/L and was significantly reduced at concentrations of 100 mg/L iodine or more. We calculated the concentrations of iodine that killed 50% of the Atlantic salmon eggs at eye-up and hatch to be 175 and 85 mg/L, respectively. Aeromonas veronii, A. schubertii, A. hydrophila, A. caviae, Plesiomonas shiggeloides , and Citrobacter spp. were the predominant bacteria present on the surface of green eggs and were significantly reduced by an iodine immersion. The use of iodine as a disinfectant on Atlantic salmon eggs was effective at low concentrations (50–75 mg/L), for which toxicity to Atlantic salmon was minimal.

North American Journal of Aquaculture↗
USGS2009

Thiamine content of eggs and lengths of coho salmon (Oncorhynchus kisutch) in relation to abundance of alewife (Alosa pseudoharengus) in eastern Lake ontario, 2003 to 2006

Early mortality syndrome in fry of Great Lakes salmonines is linked to reduced levels of thiamine in eggs, which reflects maternal consumption of forage fishes such as alewife (Alosa pseudoharengus) that contain thiaminase, an enzyme that destroys thiamine. We assessed annual variations in abundance and condition of alewives and thiamine status of coho salmon (Oncorhynchus kisutch) in Lake Ontario. We analyzed total thiamine in eggs of 20 coho salmon collected annually between 2003 and 2006 at the Salmon River Hatchery on the Salmon River, New York. Alewife abundance was assessed annually in southern and eastern Lake Ontario with bottom trawls during late April and early May. Mean thiamine concentration in eggs varied annually, with those collected in 2003 (2.5 nmol/g) being significantly higher than those collected in 2004 to 2006 (1.5 to 1.7 nmol/g). Although we did not test survival of fry, if reported threshold levels of thiamine for preventing mortality of Lake Michigan coho salmon fry apply, then many or most Lake Ontario coho salmon produced fry were likely to incur thiamine-deficiency mortality, especially during years 2004 to 2006. Comparison to indices of annual abundance of alewife in Lake Ontario with thiamine concentration in coho salmon eggs failed to show any significant correlations (P > 0.05). However, total length of female spawning coho salmon was positively correlated (P < 0.05) with increasing condition and estimated energy content of adult alewives in the previous spring. These results suggest that growth of coho salmon in Lake Ontario was first limited by energy intake, whereas the amount of thiamine provided by alewives was sufficient for growth (in length) but not for producing thiamine-adequate eggs.

East Lake Ontario↗
USGS2008

Effectiveness and retention of thiamine and its analogs administered to steelhead and landlocked Atlantic salmon

We investigated the feasibility of enhancing the reproduction of steelhead Oncorhynchus mykiss and landlocked Atlantic salmon Salmo salar in lakes where the consumption of alewives Alosa pseudoharengus and other forage fishes containing thiaminase can cause them to become thiamine deficient and thereby reduce the survival of their fry. We evaluated feeding fingerling steelhead excess thiamine hydrochloride (THCl) for 1 or 2 weeks or equimolar amounts of thiamine mononitrate, thiamine-tetrahydrofurfuryl-disulfide, benfotiamine, or dibenzoyl thiamine (DBT). We found minimal internal reserves of thiamine after 6 months. We also compared the ability of injections of thiamine and its analogs to prevent mortality in thiamine-deficient steelhead and Atlantic salmon sac fry and found all forms to be effective, although benfotiamine was the least effective on an equimolar basis. Further, we injected yearling steelhead and found that DBT was tolerated at approximately 11,200 nmol/g of body weight, about 10 times more than thiamine in any other form. When yearling steelhead were injected with near-maximal doses of thiamine hydrochloride and several analogs and then fed a thiamine-deficient diet, DBT was retained for approximately 2 years - in contrast to other forms, which were retained for less than about 6 months. Therefore, these results suggest that neither feeding nor injecting young hatchery salmonids with DBT is likely to enhance their reproduction for more than 2 years after stocking. However, injecting DBT in nearly mature fish (either cultured fish from hatcheries or wild fish captured in lakes) may provide them with enough thiamine to successfully spawn within 2 years even though they consume mainly thiaminase-containing forage fishes. ?? Copyright by the American Fisheries Society 2008.

Journal of Aquatic Animal Health↗
USGS2005

The effect of thiamine injection on upstream migration, survival, and thiamine status of putative thiamine-deficient coho salmon

A diet containing a high proportion of alewives Alosa pseudoharengus results in a thiamine deficiency that has been associated with high larval salmonid mortality, known as early mortality syndrome (EMS), but relatively little is known about the effects of the deficiency on adults. Using thiamine injection (50 mg thiamine/kg body weight) of ascending adult female coho salmon Oncorhynchus kisutch on the Platte River, Michigan, we investigated the effects of thiamine supplementation on migration, adult survival, and thiamine status. The thiamine concentrations of eggs, muscle (red and white), spleen, kidney (head and trunk), and liver and the transketolase activity of the liver, head kidney, and trunk kidney of fish injected with thiamine dissolved in physiological saline (PST) or physiological saline only (PS) were compared with those of uninjected fish. The injection did not affect the number of fish making the 15-km upstream migration to a collection weir but did affect survival once fish reached the upstream weir, where survival of PST-injected fish was almost twice that of controls. The egg and liver thiamine concentrations in PS fish sampled after their upstream migration were significantly lower than those of uninjected fish collected at the downstream weir, but the white muscle thiamine concentration did not differ between the two groups. At the upper weir, thiamine levels in the liver, spleen, head kidney, and trunk kidney of PS fish were indistinguishable from those of uninjected fish (called "wigglers") suffering from a severe deficiency and exhibiting reduced equilibrium, a stage that precedes total loss of equilibrium and death. For PST fish collected at the upstream weir, total thiamine levels in all tissues were significantly elevated over those of PS fish. Based on the limited number of tissues examined, thiamine status was indicated better by tissue thiamine concentration than by transketolase activity. The adult injection method we used appears to be a more effective means of increasing egg thiamine levels than immersion of eggs in a thiamine solution. ?? Copyright by the American Fisheries Society 2005.

Journal of Aquatic Animal Health↗
USGS1998

Effect of dietary amprolium on tissue thiamand Cayuga syndrome Atlantic salmon

Broodfish of Atlantic salmon Salmo salar were fed either thiamin-deficient diets fortified with 0.05% and 0.1% amprolium chloride (Amp, a thiamin antagonist) or thiamin-sufficient diets (0% Amp and ASD2-30, a U.S. Fish and Wildlife Service open-formula feed) for 6 months before spawning. We observed Cayuga syndrome (CS) only in the fry produced from broodfish fed the amprolium diets. The percent fry mortality due to CS was less than 1% in the 0.05% Amp group and was more than 3% in the 0.1% Amp group. Free thiamin was the predominant form of thiamin in the eggs of all broodfish; concentrations were two orders of magnitude greater than that of thiamin pyrophosphate (TPP) and thiamin monophosphate (TMP). Free thiamin was greatest in eggs of broodfish fed the thiamin-sufficient diets. Total thiamin and TMP in red blood cells (RBCs) of broodfish fed the thiamin-deficient diets were reduced to the initial levels by the end of the study. Levels of free thiamin in RBCs were similar in all treatment groups at spawning, as were levels of TPP. Amprolium feeding significantly reduced total thiamin levels in heart, liver, and muscle but not in kidney. Except for thiamin found in eggs, TPP was the predominant form of thiamin in all the other tissues, whereas free thiamin was the least predominant. The data support the hypothesis that thiamin deficiency may cause Cayuga syndrome in Atlantic salmon sac fry. The low incidence of the syndrome in this study may be due to the short duration of the feeding trial or to the low levels of amprolium added to the diets.

Transactions of the American Fisheries Society↗
USGS1994

Requirement of rainbow trout for dietary phosphorus and its relationship to the amount discharged hatchery effluents

Fingerling rainbow trout Oncorhynchus mykiss with initial mean weights of 9 g (small fish) and 35 g (large fish) were fed diets adequate in all known nutrients except phosphorus (P). In two experiments, triplicate lots of rainbow trout were fed basal diets containing either 0.14 or 0.41% non‐phytin P, with or without graded levels of supplemental P. Deficiency of P reduced growth, feed efficiency (weight gained/weight fed), bone ash, and whole‐body ash contents. The requirement for non‐phytin P by small trout for maximum growth and feed efficiency was not more than 0.41% of diet: The requirement by large trout was between 0.34 and 0.54% of diet. The requirement of non‐phytin P for maximum bone ash development was about 0.51% of diet for small trout and more than 0.54% for large trout. Whole‐body phosphorus content of small trout suggested a requirement above 0.51% but not more than 0.61% non‐phytin phosphorus. Although an effect of size of trout on the requirement was not clearly demonstrated, these results show that trout required more dietary P for bone mineralization than for weight gain. The minimum dietary requirement for non‐phytin P for bone mineralization was probably between 0.54 and 0.61% of diet. Discharges of P into effluent water increased significantly as trout were fed increasing levels of P. When trout were fed 0.61% available P, approximately 67% of P consumed was retained, and discharges of soluble P in effluents were 2.0 g P/kg weight gain or 1.8 g P/kg feed fed.

Transactions of the American Fisheries Society↗
USGS1993

Influence of phosphorus rainbow trout diets on phosphorus discharges effluent water

Two experiments were conducted with rainbow trout Oncorhynchus mykiss to investigate the influence of several diets and supplemental phosphorus on discharges of phosphorus in hatchery effluent water. A diet was formulated to contain no fish meal and a reduced level of non‐phytin phosphorus (approximately 0.9%) provided, in part, by supplemental defluorinated rock phosphate, which has a low solubility in water. Feeding rainbow trout this diet supported 86% of the growth attained with a conventional hatchery diet while discharges of phosphorus in the effluents decreased by 40–51%.

Transactions of the American Fisheries Society↗
USGS1988

High calcium concentration in water increases mortality of salmon and trout eggs

Several experiments were conducted to investigate the effect of water chemistry during water hardening on survival of eggs of Atlantic salmon ( Salmo salar ), rainbow trout ( Salmo gairdneri ), and. brook trout (Salvelinus fontinalis). Results of these experiments showed that survival was very low when eggs were exposed to very hard water containing high concentrations of calcium (approximately 520 mg/L or greater) during the first few hours of water hardening. Such high concentrations of calcium were associated with gypsum (calcium sulfate) in the water supply. In contrast, survival of eggs significantly increased when they were initially water‐hardened (1–3 h) in softer water (Ca concentrations, 34–64 mg/L). Incubation of eggs in high‐calcium water after the initial water‐hardening period did not significantly affect survival. Results of another experiment on water hardening of rainbow trout eggs showed a significant increase in survival when eggs were initially water‐hardened in either low‐gypsum water or in high‐gypsum water softened by ion exchange to markedly reduce the calcium; but not the sulfate, content.

Progressive Fish-Culturist↗
USGS1983

Requirement for dietary lysine and arginine by fry of rainbow trout

Triplicate lots of fry of rainbow trout (Salmo gairdneri) were fed a basal diet (47% protein) containing 67% corn gluten meal with and without a mixture of essential amino acids. The composition of the mixture was based on the differences between the amino acid contents of corn gluten meal and trout eggs. Fry fed the basal diet without added essential amino acids gained little weight, suffered high mortality and had eroded caudal fins. Addition of the mixture of amino acids (lysine, arginine, histidine, isoleucine, threonine, valine and tryptophan) significantly improved weight gain and prevented nearly all mortality and fin erosion. Single deletions of the amino acids valine, tryptophan, threonine, histidine and isoleucine had no significant effect on weight gain but the deletion of lysine alone significantly reduced weight gain and caused fin erosion and mortality. Deletion of arginine reduced weight gain (P<.05), but did not cause mortality or fin erosion. Feeding graded levels of each of these two amino acids showed that the minimum lysine requirement for maximum growth was about 6.1% of protein, and the minimum arginine requirement was between 5.4 and 5.9% of protein. The lysine requirements for prevention of fin erosion and mortality appeared to be lower than that for maximum weight gain.

Journal of Animal Science↗