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Gary A. Wedemeyer

Publications and source records attributed to Gary A. Wedemeyer.

11 recordsLinked to original sources

Seventy-five years of science—The U.S. Geological Survey’s Western Fisheries Research Center

As of January 2010, 75 years have elapsed since Dr. Frederic Fish initiated the pioneering research program that would evolve into today’s Western Fisheries Research Center (WFRC). Fish began his research working alone in the basement of the recently opened Fisheries Biological Laboratory on Lake Union in Seattle, Washington. WFRC’s research began under the aegis of the U.S. Fish and Wildlife Service and ends its first 75 years as part of the U.S. Geological Survey with a staff of more than 150 biologists and support personnel and a heritage of fundamental research that has made important contributions to our understanding of the biology and ecology of the economically important fish and fish populations of the Nation. Although the current staff may rarely stop to think about it, WFRC’s antecedents extend many years into the past and are intimately involved with the history of fisheries conservation in the Western United States. Thus, WFRC Director Lyman Thorsteinson asked me to write the story of this laboratory “while there are still a few of you around who were here for some of the earlier years” to document the rich history and culture of WFRC by recognizing its many famous scientists and their achievements. This historyalso would help document WFRC’s research ‘footprint’ in the Western United States and its strategic directions. Center Director Thorsteinson concluded that WFRC’s heritage told by an emeritus scientist also would add a texture of legitimacy based on personal knowledge that will all-to-soon be lost to the WFRC and to the USGS. The WFRC story is important for the future as well as for historical reasons. It describes how we got to the place we are today by documenting the origin, original mission, and our evolving role in response to the constantly changing technical information requirements of new environmental legislation and organizational decision-making. The WFRC research program owes its existence to the policy requirements of Federal conservation legislation originating with the construction of Grand Coulee Dam in 1933. The research program was shaped by laws enacted in subsequent years such as the Federal Water Pollution Control Act (1972), National Environmental Policy Act (1973), Endangered Species Act (1974), and Northwest Power Planning Act (1980), to name only a few. The WFRC has not been constrained by direct management or regulatory responsibility for a particular fishery (such as providing sustainable catch limits data to a resource management structure). Thus, WFRC has been able to concentrate on scientific pursuits and information needs required by contemporary environmental legislation. Over the years, we have pioneered in several important areas of fisheries research including the diagnoses and control of diseases in economically important fish, effects of environmental alterations on the physiological quality and survival of Pacific salmon released from federal mitigation hatcheries, applications in biotelemetry, and the bioenergetics of predator-prey interactions in the Columbia River. The WFRC of today is a widely distributed organization in the Western United States. Knowledge of the historical connections and accomplishments of our predecessors is important beyond the sense of pride and unity it instills in the WFRC family of today. For example, a discerning reader will note the evolution of WFRC’s research from a single disciplinary focus (early era—hatchery disease problems), to multiple disciplines (middle to late era—species, populations, habitats; threatened and endangered species), to the present era (multidisciplinary and with increasing process focus). For the benefit of the current WFRC staff, more emphasis has been placed on the early years rather than on the present day because people are quite naturally more familiar with the recent past than with the research done during the first decades of WFRC’s existence. By every rational measure, the WFRC has evolved into a fisheries research organization well positioned to provide the biological information needed to support the continued conservation and management of our Nation’s living aquatic natural resources. The high standard of excellence that connects WFRC’s past to our present research program provides a firm foundation on which to base the work yet to be done. In another 75 years, WFRC will undoubtedly be a very different place than it is today, but its evolution will be forever rooted in the story of the research and of the people related here. More about the diverse fisheries research projects WFRC scientists are conducting today is available at WFRC’s website: http://wfrc.usgs.gov/.

Washington

Physiological response of largemouth bass to angling stress

The physiological effects of catch-and-release fishing on largemouth bass Micropterus salmoides from Lake Powell and Mantua Reservoir, Utah, were evaluated, and an estimate of the time needed for recovery from hooking stress was obtained. Fatigue in Lake Powell fish, as indicated by elevated blood lactate, was directly proportional to hooking time (1–5 min) and water temperature, but recovery from the hyperlacticemia was relatively rapid (about 24 h). Hyperglycemia, an indicator of stress hormone production, did not occur in largemouth bass hooked and played for 1–5 min in the coldest water (11–13°C), was moderate in fish hooked and played at l6–20°C, and was severe in fish played for 5 min at 28–30°C. Fish held for recovery in live cages suffered further hyperglycemia, presumably because of the stress of confinement. Ionoregulation, as indicated by relatively stable plasma chloride values, was not immediately affected in largemouth bass caught at water temperatures of 11–13°C or 28–30°C, but an unusual hyperchloremia developed in fish hooked and played at 16–20°C. During recovery, the expected progressive hypochloremia developed. Plasma osmolality was somewhat affected by hooking at all water temperatures tested, but recovery was almost complete within about 8 h. Mantua Reservoir fish were hooked and played only at water temperatures of 23–26°C. The hyperlacticemia and hyperglycemia that occurred were generally more severe than in the Lake Powell fish hooked and played at either 16–20°C or 28–30°C. However, effects on plasma chloride and osmolality were similar to those occurring in Lake Powell fish.

Transactions of the American Fisheries Society

Tolerance of developing salmonid eggs and fry to nitrate exposure

This paper reports on tests which show significant effects on early salmonid life stages of nitrates at levels commonly found in groundwaters in geographical areas that are influenced by fertilizer application. It has long been known, from fish cultural experience, that in certain site specific locations, chronic problems can be expected with salmonid egg development and early fry mortality. However, fingerlings which survive usually grow normally. A complete explanation is lacking although several environmental factors have been proposed to account for this phenomenon. One, which has so far received little attention, is that nitrate levels in the ground and surface waters of many areas have been increasing significantly over historical background levels. Ammonia, urea, and other potential sources of nitrate can enter natural waters from a variety of sources, such as domestic or industrial sewage, animal feedlots, or seepage and return flows from agricultural lands. The latter may be the largest contributor, since billions of tons of nitrate fertilizers are applied to agricultural crops on a worldwide basis each year. In addition, intensive forest management techniques include the aerial application of nitrate fertilizer to increase the yield of wood products, while range management practices call for use of nitrates to increase forage production. The nitrate that is not taken up by plants ultimately appears in ground or surface waters.

Bulletin of Environmental Contamination and Toxico

Potentials and limits for the use of ozone as a fish disease control agent

Ozone and chlorine inactivation curves were determined in three types of freshwater at 20 C for the destruction of the fish pathogens Aeromonas salmonicida the etiologic agent of furunculosis, and Yersinia ruckeri the enteric redmouth bacterium (ERM). Ozone and chlorine inactivation curves were also obtained in the same water types at 10 C for the fish pathogenic viruses infectious hematopoietic necrosis (IHNV), and infectious pancreatic necrosis (IPNV). Acute toxicity tests using the rainbow trout as a representative salmonid revealed that ozone was highly toxic at the dose levels used. Partial chronic (3. mo.) testing revealed that ozone exposure at 2 μg/L causes only minimal physiological changes, none of which would be expected to compromise biological function.

Ozone: Science and Engineering

Survival of two bacterial fish pathogens (Aeromonas salmonicida and the Enteric Redmouth Bacterium) in ozonated, chlorinated, and untreated waters

Ozone and chlorine inactivation curves were determined in three water types at 20 °C for the destruction of the fish pathogens Aeromonas salmonicida , the etiologic agent of furunculosis, and the enteric redmouth bacterium (ERM). In phosphate-buffered distilled water, 0.01 mg/ℓ ozone inactivated 10 3 cells/ml of ERM and A . salmonicida in 1/2 and 10 min, respectively. Chlorine at this concentration had little effect on either pathogen and a residual of at least 0.05 mg/ℓ was needed to achieve a complete kill within a 10-min contact time. In soft lake water (30 mg/ℓ as CaCO 3 ) a chlorine residual of 0.1 mg/ℓ rapidly inactivated A . salmonicida and ERM but in hard water (120 mg/ℓ) A . salmonicida was more resistant and 0.2 mg/ℓ chlorine was required. Ozonation of the two lake waters at 90 mg O 3 ∙h −1 ∙ℓ −1 (equivalent to a 0.01 mg/ℓ residual in ozone demand-free water) was required to destroy both pathogens within 10 min.In untreated soft lake water 10 3 cells/ml of A . salmonicida survived only 2 days, while the ERM bacterium (10 3 cells/ml) survived even after 20 day s in soft and hard untreated lake waters.

Journal of the Fisheries Research Board of Canada

Clinical methods for the assessment of the effects of environmental stress on fish health

Clinical methods are presented for biological monitoring of hatchery and native fish populations to assess the effects of environmental stress on fish health. The choice of methods is based on the experience of the authors and the judgment of colleagues at fishery laboratories of the U.S. Fish and Wildlife Service. Detailed analysis methods, together with guidelines for sample collection and for the interpretation of results, are given for tests on blood (cell counts, chloride, cholesterol, clotting time, cortisol, glucose, hematocrit, hemoglobin, lactic acid, methemoglobin, osmolality, and total protein); water (ammonia and nitrite content); and liver and muscle (glycogen content).

Technical Paper

Physiological response of juvenile coho salmon (Oncorhynchus kisutch) and rainbow trout (Salmo gairdneri) to handling and crowding stress in intensive fish culture

Moving 4–5-in. coho salmon ( Oncorhynchus kisutch ) held in soft (20 ppm CaCO 3 ) water from the relatively light loading density of 0.5 lb/ft 3 to 1, 2, or 4 lb/ft 3 (density index, DI = 0.1, 0.2, 0.4, 0.8) caused significant stress as indicated by loss of feeding behavior, but only minimal physiological disturbances, as indicated by lack of hyperglycemia or hypochloremia. However, moving them to 6 or 12 lb/ft 3 (DI = 1.2, 2.4) caused significant physiological stress which required at least a week for recovery. Smolting coho salmon were physiologically stressed by population densities of 1 lb/ft 3 or more and a subclinical corynebacterial kidney infection was activated. Rainbow trout ( Salmo gairdneri ) (4–5 in.) were physiologically stressed when moved and held at 1 lb/ft 3 or more but retained normal feeding behavior. This indicates that handling and crowding stress will be minimized in softwater areas if densities in fish distribution trucks or in ponds or raceways during disease treatments are held to 0.1–0.5 lb/gal.

Journal of the Fisheries Research Board of Canada

Statistical methods for estimating normal blood chemistry ranges and variance in rainbow trout (Salmo gairdneri), Shasta Strain

Gaussian and nonparametric (percentile estimate and tolerance interval) statistical methods were used to estimate normal ranges for blood chemistry (bicarbonate, bilirubin, calcium, hematocrit, hemoglobin, magnesium, mean cell hemoglobin concentration, osmolality, inorganic phosphorus, and pH for juvenile rainbow ( Salmo gairdneri , Shasta strain) trout held under defined environmental conditions. The percentile estimate and Gaussian methods gave similar normal ranges, whereas the tolerance interval method gave consistently wider ranges for all blood variables except hemoglobin. If the underlying frequency distribution is unknown, the percentile estimate procedure would be the method of choice.

Journal of the Fisheries Research Board of Canada

Nutritional factors in the biochemical pathology of Corynebacterial kidney disease in the coho salmon (Oncorhynchus kisutch)

The influence of diet ingredient on the morbidity and biochemical pathogenesis of corynebacterial kidney disease was investigated using juvenile coho salmon ( Oncorhynchus kisutch ) fed the Abernathy dry ration made up with either corn gluten or cottonseed meal (isoprotein, isocaloric substitution). Evaluation of incidence of infection, pituitary activation and aspects of carbohydrate metabolism, acid-base balance, renal function, and hematopoietic activity showed that the actual disease incidence was about the same for both diets but the nonspecific stress of infection was more severe in fish fed the corn gluten.Discriminant function calculations combining four physiological parameters gave a probability of 0.86 for successfully diagnosing infected fish on the basis of these blood chemistry tests.

Journal of the Fisheries Research Board of Canada

Organochlorine insecticide, herbicide and polychlorinated biphenyl (PCB) inhibition of NaK-ATPase in rainbow trout

The current widespread presence of chlorinated insecticides, polychlorinated biphenyls (PCB's) and herbicides in world waterways has elicited much interest in the mechanisms of their toxicity in fishes. Inhibition of Na+,K+-activated adenosinetriphosphatase (NaK-ATPase) and Mg++-dependent ATPase (Mg-ATPase) by DDT, endosulfan and dicofol has been demonstrated in gill, brain and kidney microsomes of rainbow trout (1,2). Intestinal and gill ATPases in marine teleosts were recently reported to be sensitive to organochlorines (3). CutkonTp et al (4) noted inhibition of NaK-ATPase and Mg-ATPase in bluegill brain, liver, muscle and kidney by DDT and related chlorinated hydrocarbons. Inhibition of ATPases by PCB's has been recently shown in bluegill kidney, brain and liver (5). In the present study, we have further examined the NaK-ATPase enzyme system in trout gill as a site for the possible toxicity of selected organopolychlors, i.e., chlorinated insecticides, herbicides and PCB's.

Bulletin of Environmental Contamination and Toxico

Na+, K+-activated-ATPase inhibition in rainbow trout: A site for organochlorine pesticide toxicity?

1. The Na + , K + -activated, Mg 2+ -dependent-ATPase enzyme system in a heavy microsomal fraction of rainbow trout ( Salmo gairdneri ) brain was inhibited in vitro by chlorinated hydrocarbon pesticides. 2. T 50 (concentration at 50 per cent inhibition) values for dicofol, endosulfan and DDT were 5 × 10 −6 , 3 × 10 −5 and 1 × 10 −4 M respectively. Similar inhibition by these pesticides occurred in kidney and gill ATPase preparations. 3. An unexpected finding was a failure of the classic inhibitor, ouabain, to block the Na + , K + -activated component of ATPase activity in the gill. 4. It is suggested that inhibition of ATPase activity may be a causal factor in the toxic effects of organochlorine pesticides in fishes.

Comparative Biochemistry and Physiology, Part B: C