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Erin E. Bertke

Publications and source records attributed to Erin E. Bertke.

7 recordsLinked to original sources

Changes in phosphorus concentration and flux from 2011 to 2023 in major U.S. tributaries to the Laurentian Great Lakes

Reducing phosphorus (P) flux to the Great Lakes is critical for improving water quality and controlling eutrophication. We used 13 water years (2011–2023) of U.S. Geological Survey data from 24 major U.S. tributaries (representing 47% of the U.S. Great Lakes watershed area) to evaluate temporal changes in orthophosphate (PO 4 -P) and total P (TP) using Weighted Regressions on Time, Discharge, and Season. We assessed actual and flow-normalized P concentrations and fluxes. Between 2011 and 2023, P concentrations and fluxes declined in many tributaries, although the extent and significance of these declines varied. Decreases were more common and statistically likely for TP than PO 4 -P, and several high-loading watersheds had modest or non-significant changes. Flow-normalized PO 4 -P:TP flux ratios increased in over half the tributaries, suggesting that even where P reductions occurred, reductions in the more bioavailable P fraction were proportionally smaller. Actual P fluxes were strongly correlated with streamflow, and year-to-year variability in actual fluxes was, on average, three times greater than variability related to trends in flow-normalized fluxes. This underscores the role of hydrology in modulating P export and highlights how changing precipitation and runoff patterns can obscure or counteract management progress. Spring accounted for the largest share of annual P flux in most tributaries, though many showed declining spring contributions. Our basin-wide analysis reveals that while management efforts may have yielded progress in reducing TP in many watersheds, additional strategies would be needed to address PO 4 -P reductions and account for changing hydrology, especially in high-contributing watersheds.

Journal of Great Lakes Research

Testing and refining the Ohio Nowcast at two Lake Erie beaches— 2008

The Ohio Nowcast has been providing real-time beach advisories to the public on the basis of predictive models since 2006. In support of the nowcast, data were collected during the recreational season of 2008 to validate and refine predictive models at two Lake Erie beaches. Predictive models yield data on the probability that the single-sample bathing-water standard for E. coli will be exceeded. Field personnel collected or compiled data on Escherichia coli (E. coli) concentrations as well as variables expected to affect these concentrations, including manual and automated measurements of turbidity, wave height, and water temperature; lake level; and radar and airport rainfall amounts. Two new variables were measured during 2008 - photosynthetically-active radiation at Huntington (Bay Village) and foreshore head at Edgewater (Cleveland). (The foreshore is a strip of land along a body of water between low and high water marks.) The performance of the nowcast was monitored during 2008. The Huntington nowcast yielded a greater percentage of correct responses (84.9 percent) than did the previous day's E. coli concentration (75.2 percent). In contrast, at Edgewater, the nowcast yielded a slightly higher percentage of correct responses (61.0 percent) as compared to the previous day's E. coli concentration (56.5 percent), but both percentages were relatively low. Lake levels in 2008 were significantly higher than levels in the data used to develop the Edgewater models (2004-7), confounding their abilities to provide correct responses. At Edgewater during 2008, the strongest relation (as measured by Pearson's correlation) was between E. coli concentrations and the difference in foreshore head over the past 24 hours (r=0.48), a variable not included in the models. At Huntington, photosynthetically-active radiation on the previous day showed a significant negative relation to E. coli concentrations (r=-0.33) during 2008. Refined models were developed for Huntington and Edgewater using data collected from 2005-8. The refined models included the variables wave height, log turbidity, radar or airport rainfall, and day of the year in various combinations for different dated segments of the recreational season. Water-resource managers will determine which models to apply to the Ohio Nowcast for issuing water-quality advisories in 2009.

Ohio

Performance of Traditional and Molecular Methods for Detecting Biological Agents in Drinking Water

To reduce the impact from a possible bioterrorist attack on drinking-water supplies, analytical methods are needed to rapidly detect the presence of biological agents in water. To this end, 13 drinking-water samples were collected at 9 water-treatment plants in Ohio to assess the performance of a molecular method in comparison to traditional analytical methods that take longer to perform. Two 100-liter samples were collected at each site during each sampling event; one was seeded in the laboratory with six biological agents - Bacillus anthracis (B. anthracis), Burkholderia cepacia (as a surrogate for Bu. pseudomallei), Francisella tularensis (F. tularensis), Salmonella Typhi (S. Typhi), Vibrio cholerae (V. cholerae), and Cryptospordium parvum (C. parvum). The seeded and unseeded samples were processed by ultrafiltration and analyzed by use of quantiative polymerase chain reaction (qPCR), a molecular method, and culture methods for bacterial agents or the immunomagnetic separation/fluorescent antibody (IMS/FA) method for C. parvum as traditional methods. Six replicate seeded samples were also processed and analyzed. For traditional methods, recoveries were highly variable between samples and even between some replicate samples, ranging from below detection to greater than 100 percent. Recoveries were significantly related to water pH, specific conductance, and dissolved organic carbon (DOC) for all bacteria combined by culture methods, but none of the water-quality characteristics tested were related to recoveries of C. parvum by IMS/FA. Recoveries were not determined by qPCR because of problems in quantifying organisms by qPCR in the composite seed. Instead, qPCR results were reported as detected, not detected (no qPCR signal), or +/- detected (Cycle Threshold or 'Ct' values were greater than 40). Several sample results by qPCR were omitted from the dataset because of possible problems with qPCR reagents, primers, and probes. For the remaining 14 qPCR results (including some replicate samples), F. tularensis and V. cholerae were detected in all samples after ultrafiltration, B. anthracis was detected in 13 and +/- detected in 1 sample, and C. parvum was detected in 9 and +/- detected in 4 samples. Bu. cepacia was detected in nine samples, +/- detected in two samples, and not detected in three samples (for two out of three samples not detected, a different strain was used). The qPCR assay for V. cholerae provided two false positive - but late - signals in one unseeded sample. Numbers found by qPCR after ultrafiltration were significantly or nearly significantly related to those found by traditional methods for B. anthracis, F. tularensis, and V. cholerae but not for Bu. cepacia and C. parvum. A qPCR assay for S. Typhi was not available. The qPCR method can be used to rapidly detect B. anthracis, F. tularensis, and V. cholerae with some certainty in drinking-water samples, but additional work would be needed to optimize and test qPCR for Bu. cepacia and C. parvum and establish relations to traditional methods. The specificity for the V. cholerae assay needs to be further investigated. Evidence is provided that ultrafiltration and qPCR are promising methods to rapidly detect biological agents in the Nation's drinking-water supplies and thus reduce the impact and consequences from intentional bioterrorist events. To our knowledge, this is the first study to compare the use of traditional and qPCR methods to detect biological agents in large-volume drinking-water samples.

Scientific Investigations Report

Comparison of the Immunomagnetic Separation/Adenosine Triphosphate Rapid Method and the Modified mTEC Membrane-Filtration Method for Enumeration of Escherichia coli

Water quality at beaches is monitored for fecal indicator bacteria by traditional, culture-based methods that can take 18 to 24 hours to obtain results. A rapid detection method that provides estimated concentrations of fecal indicator bacteria within 1 hour from the start of sample processing would allow beach managers to post advisories or close the beach when the conditions are actually considered unsafe instead of a day later, when conditions may have changed. A rapid method that couples immunomagnetic separation with adenosine triphosphate detection (IMS/ATP rapid method) was evaluated through monitoring of Escherichia coli (E. coli) at three Lake Erie beaches in Ohio (Edgewater and Villa Angela in Cleveland and Huntington in Bay Village). Beach water samples were collected between 4 and 5 days per week during the recreational seasons (May through September) of 2006 and 2007. Composite samples were created in the lab from two point samples collected at each beach and were shown to be comparable substitutes for analysis of two individual samples. E. coli concentrations in composite samples, as determined by the culture-based method, ranged from 4 to 24,000 colony-forming units per 100 milliliters during this study across all beaches. Turbidity also was measured for each sample and ranged from 0.8 to 260 neophelometric turbidity ratio units. Environmental variables were noted at the time of sampling, including number of birds at the beach and wave height. Rainfall amounts were measured at National Weather Service stations at local airports. Turbidity, rainfall, and wave height were significantly related to the culture-based method results each year and for both years combined at each beach. The number of birds at the beach was significantly related to the culture-based method results only at Edgewater during 2006 and during both years combined. Results of the IMS/ATP method were compared to results of the culture-based method for samples by year for each beach. The IMS/ATP method underwent several changes and refinements during the first year, including changes in reagents and antibodies and alterations to the method protocol. Because of the changes in the method, results from the two years of study could not be combined. Kendall's tau correlation coefficients for relations between the IMS/ATP and culture-based methods were significant except for samples collected during 2006 at Edgewater and for samples collected during 2007 at Villa Angela. Further, relations were stronger for samples collected in 2006 than for those collected in 2007, except at Edgewater where the reverse was observed. The 2007 dataset was examined to identify possible reasons for the observed difference in significance of relations by year. By dividing the 2007 data set into groups as a function of sampling date, relations (Kendall's tau) between methods were observed to be stronger for samples collected earlier in the season than for those collected later in the season. At Edgewater and Villa Angela, there were more birds at the beach at time of sampling later in the season compared to earlier in the season. (The number of birds was not examined at Huntington.) Also, more wet days (when rainfall during the 24 hours prior to sampling was greater than 0.05 inch) were sampled later in the season compared to earlier in the season. Differences in the dominant fecal source may explain the change in the relations between the culture-based and IMS/ATP methods.

Scientific Investigations Report

Models for predicting recreational water quality at Lake Erie beaches

Data collected from four Lake Erie beaches during the recreational seasons of 2004-05 and from one Lake Erie beach during 2000-2005 were used to develop predictive models for recreational water quality by means of multiple linear regression. The best model for each beach was based on a unique combination of environmental and water-quality explanatory variables including turbidity, rainfall, wave height, water temperature, day of the year, wind direction, and lake level. Two types of outputs were produced from the models: the predicted Escherichia coli concentration and the probability that the bathing-water standard will be exceeded. The model for one of beaches, Huntington Reservation (Huntington), was validated in 2005. For 2005, the Huntington model yielded more correct responses and better predicted exceedance of the standard than did current methods for assessing recreational water quality, which are based on the previous day's E. coli concentration. Predictions based on the Huntington model have been available to the public through an Internet-based 'nowcasting' system since May 30, 2006. The other beach models are being validated for the first time in 2006. The methods used in this study to develop and test predictive models can be applied at other similar coastal beaches.

Scientific Investigations Report

Use of Spatial Sampling and Microbial Source-Tracking Tools for Understanding Fecal Contamination at Two Lake Erie Beaches

Source-tracking tools were used to identify potential sources of fecal contamination at two Lake Erie bathing beaches: an urban beach (Edgewater in Cleveland, Ohio) and a beach in a small city (Lakeshore in Ashtabula, Ohio). These tools included identifying spatial patterns of Escherichia coli (E. coli) concentrations in each area, determining weather patterns that caused elevated E. coli, and applying microbial source tracking (MST) techniques to specific sites. Three MST methods were used during this study: multiple antibiotic resistance (MAR) indexing of E. coli isolates and the presence of human-specific genetic markers within two types of bacteria, the genus Bacteroides and the species Enterococcus faecium. At Edgewater, sampling for E. coli was done during 2003-05 at bathing-area sites, at nearshore lake sites, and in shallow ground water in foreshore and backshore areas. Spatial sampling at nearshore lake sites showed that fecal contamination was most likely of local origin; E. coli concentrations near the mouths of rivers and outfalls remote to the beach were elevated (greater than 235 colony-forming units per 100 milliliters (CFU/100 mL)) but decreased along transport pathways to the beach. In addition, E. coli concentrations were generally highest in bathing-area samples collected at 1- and 2-foot water depths, midrange at 3-foot depths, and lowest in nearshore lake samples typically collected 150 feet from the shoreline. Elevated E. coli concentrations at bathing-area sites were generally associated with increased wave heights and rainfall, but not always. E. coli concentrations were often elevated in shallow ground-water samples, especially in samples collected less than 10 feet from the edge of water (near foreshore area). The interaction of shallow ground water and waves may be a mechanism of E. coli storage and accumulation in foreshore sands. Infiltration of bird feces through sand with surface water from rainfall and high waves may be concentrating E. coli in shallow ground water in foreshore and backshore sands. At Lakeshore, sampling for E. coli was done at bathing-area, nearshore lake, and parking-lot sites during 2004-05. Low concentrations of E. coli at nearshore lake sites furthest from the shoreline indicated that fecal contamination was most likely of local origin. High concentrations of E. coli in water and bed sediments at several nearshore lake sites showed that contamination was emanating from several points along the shoreline during wet and dry weather, including the boat ramp, an area near the pond drainage, and parking-lot sediments. Physical evidence confirmed that runoff from the parking lot leads to degradation of water quality at the beach. MST samples were collected to help interpret spatial findings and determine whether sources of fecal contamination were from wastewater or bird feces and if a human-specific marker was present. MAR indices were useful in distinguishing between bird feces and wastewater sources because they were about 10 times higher in the latter. The results from MAR indices agreed with results from the two human-specific markers in some but not all of the samples tested. Bacteroides and enterococci human-specific markers were found on one day at Edgewater and two days at Lakeshore. On three days at Edgewater and two days at Lakeshore, the MAR index indicated a mixed source, but neither marker was found in bathing-water samples; this may be because bacterial indicator concentrations were too low to detect a marker. Multiple tools are needed to help identify sources of fecal contamination at coastal beaches. Spatial sampling identified patterns in E. coli concentrations and yielded information on the physical pathways of contamination. MST methods provided information on whether the source was likely of human or nonhuman origin only; however, MST did not provide information on the pathways of contamination.

Scientific Investigations Report

Bacteriological water quality in the Lake Pontchartrain basin, Louisiana, following Hurricanes Katrina and Rita, September 2005

The U.S. Geological Survey (USGS), in collaboration with the Louisiana Department of Environmental Quality, monitored bacteriological quality of water at 22 sites in and around Lake Pontchartrain, La., for three consecutive weeks beginning September 13, 2005, following hurricanes Katrina and Rita and the associated flooding. Samples were collected and analyzed by USGS personnel from the USGS Louisiana Water Science Center and the USGS Ohio Water Microbiology Laboratory. Fecal-indicator bacteria (Escherichia coli, enterococci, and fecal coliform) concentrations ranged from the detection limit to 36,000 colony-forming units per 100 milliliters. Data are presented in tabular form and as plots of data in the context of available historical data and water-quality standards and criteria for each site sampled. Quality-control data were reviewed to ensure that methods performed as expected in a mobile laboratory setting.

Louisiana