The respiratory arsenate reductase from Bacillus selenitireducens strain MLS10
The respiratory arsenate reductase from the Gram-positive, haloalkaliphile, Bacillus selenitireducens strain MLS10 was purified and characterized. It is a membrane bound heterodimer (150 kDa) composed of two subunits ArrA (110 kDa) and ArrB (34 kDa), with an apparent K m for arsenate of 34 µM and V max of 2.5 µmol min −1 mg −1 . Optimal activity occurred at pH 9.5 and 150 g l −1 of NaCl. Metal analysis (inductively coupled plasma mass spectrometry) of the holoenzyme and sequence analysis of the catalytic subunit (ArrA; the gene fr which was cloned and sequenced) indicate it is a member of the DMSO reductase family of molybdoproteins.
FEMS Microbiology Letters↗