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C. P. Carlson

Publications and source records attributed to C. P. Carlson.

3 recordsLinked to original sources

Infectious pancreatic necrosis virus: Lyophilization and subsequent stability in storage at 4 C

Infectious pancreatic necrosis virus (ATCC strain VR # 299) was lyophilized with different additives and stored at 4 C. Ampoules were assayed at 2 days, at 1, 3, and 6 months, and at 1, 3, and 4 years. Processing losses were least and subsequent maintenance of infectivity was best in skim milk, lactalbumin hydrolysate, and lactose. Quantitative data are given in tabular form.

Applied Microbiology

Infectious pancreatic necrosis: Selection of virus-free stock from a population of carrier trout

Infectious pancreatic necrosis (IPN) is a virulent disease of young trouts and is easily transmitted from infected animals through water and with eggs. At present, the most effective control measure consists of propagation of specific pathogen-free stock. Methods are described for using fish cell cultures to detect IPN virus in peritoneal washes, feces, and gonadal fluids and thereby to identifiy carrier and non-carrier fish. The procedures were used to derive virus-free progeny from a hatchery trout population containing about one-third carrier fish. The progeny were reared to maturity, spawned for 3 successive years, and at each spawning they proved free of virus. Recommendations and precautions are given for those who may wish to apply the procedures.

Journal of the Fisheries Research Board of Canada