Geology topics

Bruce McCune

Publications and source records attributed to Bruce McCune.

3 recordsLinked to original sources

USGS2025

DNA metabarcoding of biocrust lichen-forming fungi detects responses to disturbance and invasion

Biocrusts dominated by bryophytes and lichens perform important functions in dryland ecosystems but monitoring these communities can be cost prohibitive over broad scales. We explored DNA metabarcoding as a potential tool for monitoring biocrust lichen communities at a site in Washington (U.S.A.) that had already been surveyed for lichen diversity and community composition. We developed a DNA reference library using the internal transcribed spacer (ITS) region from specimens collected in the study area. We also visually estimated the abundance of lichen species or groups in 22 plots spanning a range of wildfire history and invasion by the exotic annual grass Bromus tectorum . At these plots, we collected bulk lichen community samples for metabarcoding using two approaches: small sample dishes and combined biocrust fragments collected using tweezers from all species observed. We used PacBio sequencing to simultaneously generate ITS barcode sequences for all lichen-forming fungi (LFF) present in the bulk samples, clustering similar sequences into operational taxonomic units (OTUs). Lichen communities detected visually and using DNA metabarcoding both captured a reduction of biocrust diversity and change in community composition related to the abundance of B. tectorum , suggesting that metabarcoding was able to identify the same dominant ecological pattern in biocrust lichens as visual sampling. The tweezer sampling approach captured on average 12.3 more OTUs than the dish approach and some taxa were more consistently detected by one approach or the other. After using the specimen DNA reference library to identify species associated with LFF OTUs, we determined that metabarcoding and visual sampling detected overlapping but different lichen species. Metabarcoding failed to detect common collected taxa, including: Arthonia glebosa, Candelariella spp ., Enchylium tenax, Lecanora muralis, Lecidella spp ., Leptochidium albociliatum, Massalongia carnosa, and Trapeliopsis glaucopholis. However, metabarcoding detected OTUs not visually observed in the genera Elixia, Lecanora, Lecanoropsis, Bacidina, Pyrenodesmia, Xanthocarpia, Trapelia, Verrucaria, and Verruculopsis . Furthermore, metabarcoding identified greater OTU diversity than expected within Diploschistes muscorum, Trapeliopsis bisorediata, and Trapeliopsis steppica . Our results suggest that metabarcoding alone or combined with visual methods could be a useful approach for monitoring biocrust lichen communities and their response to disturbance, invasion, and potential restoration.

Washington↗

USGS2023

Long-term biocrust responses to wildfires in Washington, USA

Premise Dryland ecosystems in the western United States are affected by invasive species, wildfires, livestock grazing, and climate change in ways that are difficult to distinguish. Biocrusts perform important ecological roles in these systems and are sensitive to all of these pressures. Methods We revisited a Washington, USA, site sampled for biocrusts in 1999 to focus on effects of exotic annual grass invasion and wildfires in the absence of livestock grazing. We examined changes between 1999 and 2020 using a Bayesian directed acyclic graph (DAG) to interpret direct and indirect causal impacts of wildfire on perennial bunchgrasses, exotic annual grasses, and biocrusts. Results Between 1999 and 2020, exotic annual grass cover increased in all plots and in unburned plots by 16% and 18%, respectively, bunchgrass cover decreased by 21% and 25%, and biocrust cover decreased by 8.9% and 9.8%. Our DAG suggested that decreases in bunchgrass increased exotic annual grass, which reduced biocrust cover. Wildfires did not directly influence changes in bunchgrass, exotic annual grass, or biocrust cover. Areas dominated by exotic annual grass had less abundant and diverse biocrusts than areas with less exotic annual grass. Conclusions Biocrust community changes were more strongly related to increasing exotic annual grasses than to wildfires. Changes may relate to other soil disturbances or broad-scale changes in climate or air quality. The minimal influence of wildfire on exotic annual grass and biocrusts suggests that apparent negative impacts of wildfire at other sites may be due to exacerbation by livestock grazing or other surface disturbance.

American Journal of Botany↗

USGS2015

Response of the nitrogen-fixing lichen Lobaria pulmonaria to phosphorus, molybdenum, and vanadium

Nitrogen-fixing lichens (cyanolichens) are an important source of nitrogen (N) in Pacific Northwest forests, but limitation of lichen growth by elements essential for N fixation is poorly understood. To investigate how nutrient limitation may affect cyanolichen growth rates, we fertilized a tripartite cyanobacterial lichen ( Lobaria pulmonaria ) and a green algal non-nitrogen fixing lichen ( Usnea longissima ) with the micronutrients molybdenum (Mo) and vanadium (V), both known cofactors for enzymes involved in N fixation, and the macronutrient phosphorus (P). We then grew treated lichens in the field for one year in western Oregon, USA. Lichen growth was very rapid for both species and did not differ across treatments, despite a previous demonstration of P-limitation in L. pulmonaria at a nearby location. To reconcile these disparate findings, we analyzed P, Mo, and V concentrations, natural abundance δ 15 N isotopes, %N and change in thallus N in Lobaria pulmonaria from both growth experiments. Nitrogen levels in deposition and in lichens could not explain the large difference in growth or P limitation observed between the two studies. Instead, we provide evidence that local differences in P availability may have caused site-specific responses of Lobaria to P fertilization. In the previous experiment, Lobaria had low background levels of P, and treatment with P more than doubled growth. In contrast, Lobaria from the current experiment had much higher background P concentrations, similar to P-treated lichens in the previous experiment, consistent with the idea that ambient variation in P availability influences the degree of P limitation in cyanolichens. We conclude that insufficient P, Mo, and V did not limit the growth of either cyanolichens or chlorolichens at the site of the current experiment. Our findings point to the need to understand landscape-scale variation in P availability to cyanolichens, and its effect on spatial patterns of cyanolichen nutrient limitation and N fixation.

Oregon↗