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Barnaby J. Watten

Publications and source records attributed to Barnaby J. Watten.

At least 19 recordsLinked to original sources

Comparison of attraction, entrance and passage of downstream migrant American eels (Anguilla rostrata) through airlift and siphon deep entrance bypass systems

Downstream migrating anguillid eels face many barriers including turbines and pumps at impoundments for water abstraction, power generation and water level control, when attempting to exit the freshwater catchment to reach spawning grounds. Multiple eel species worldwide are facing different levels of endangerment and alleviating the impacts of barriers to migration is essential to allow completion of the life cycle. Deep bypass systems with entrances located near the riverbed hold some promise for increased effectiveness compared to traditional downstream guidance and bypass facilities with entrances near the surface, as eels typically occupy the bottom of the water column. Here we evaluate two deep entrance bypass designs; an airlift (the Conte Airlift) and a conventional gravity siphon of the same entrance dimensions. Tests were performed using migratory silver-phase American eels ( Anguilla rostrata ), at night, in a simulated forebay environment. Passage performance was monitored over a 3 h test period using both PIT (passive integrated transponder) tag and video recording equipment. Entrance velocity was fixed at 1.2 m s −1 in each of 8 test runs with cohort size fixed in six runs at 14 and in two runs at 42. Test eels readily located, entered and passed both bypass designs. Differences in performance metrics between the airlift and siphon were not statistically significant ( P > 0.05) with linked mean values of 74.5%, 90.5% and 100%, respectively. Eel length did not affect passage speed ( P > 0.05) or slip ratio, i.e., the measured eel velocity relative to fluid velocity. The slip ratio was, however, greater in the siphon than in the airlift ( P < 0.01) within identical vertical upflow sections of the test equipment. Siphon slip ratios in the upflow vertical section were comparable to those established for the horizontal and downflow sections. Fish density did not affect attraction and passage through the airlift or siphon. No mortality or signs of injury were observed on any of the test eels through a 48 h post-test observation period. Both airlift and siphon downstream bypass systems show promise as effective technologies for protection of downstream migrating eels at a variety of water diversion or hydroelectric sites that pose threats of impingement, entrainment, and turbine mortality.

Massachusetts

Using carbon dioxide in fisheries and aquatic invasive species management

To restore native fish populations, fisheries programs often depend on active removal of aquatic invasive species. Chemical removal can be an effective method of eliminating aquatic invasive species, but chemicals can induce mortality in nontarget organisms and persist in the environment. Carbon dioxide (CO 2 ) is an emerging alternative to traditional chemical control agents because it has been demonstrated to be toxic to fish, but is naturally occurring and readily neutralized. In addition, CO 2 is a commercially available gas, is highly soluble, and has high absorption efficiency. When these characteristics are paired with advances in modern, large-scale gas delivery technologies, opportunities to use CO 2 in natural or artificial (e.g., canals) waters to manage fish become increasingly feasible. Our objective is to describe the history of CO 2 use in fisheries and outline potential future applications of CO 2 to suppress and manipulate aquatic species in field and aquaculture settings.

Fisheries

Performance and application of a fluidized bed limestone reactor designed for control of alkalinity, hardness and pH at the Warm Springs Regional Fisheries Center

Springs serving the Warm Springs Regional Fisheries Center, Warm Springs, Georgia, have pH, alkalinity, and hardness levels thatlie under the range required for successful fish propagation while free CO 2 is well above allowable targets. We evaluate a pretreatment process that exploits limestone’s (CaCO 3 ) ability to react away hydrogen ions (H + ) and carbon dioxide (CO 2 ) while increasing alkalinity (HCO 3 − ) and calcium (Ca2+) concentrations, i.e. CaCO 3 + H + ↔ HCO 3 − + Ca 2+ CaCO 3 + CO 2 + H 2 O ↔ Ca 2+ + 2HCO 3 − Limestone sand was tested in both pilot and full scale fluidized bed reactors (CycloBio®). We first established the bed expansion characteristics of three commercial limestone products then evaluated the effect of hydraulic flux and bed height on dissolution rate of a single selected product (Type A16 × 120). Pilot scale testing at 18C showed limestone dissolution rates were relatively insensitive to flux over the range 1.51–3.03 m 3 /min/m 2 but were sensitive (P < 0.001; R 2 = 0.881) to changes in bed height (BH, cm) over the range 83–165 cm following the relation: (Alkalinity, mg/L) = 123.51 − (3788.76 (BH)). Differences between filtered and non-filtered alkalinity were small(P > 0.05) demonstrating that limestone was present in the reactor effluent primarily in the form of dissolved Ca(HCO 3 ) 2 . Effluent alkalinity exceeded our target level of 50 mg/L under most operating conditions evaluated with typical pilot scale values falling within the range of 90–100 mg/L despite influent concentrations of about 4 mg/L. Concurrently, CO 2 fell from an average of 50.6 mg/L to 8.3 mg/L (90%), providing for an increase in pH from 5.27 to a mean of 7.71. The ability of the test reactor to provide changes in water chemistry variables that exceeded required changes allowed for a dilution ratio of 0.6. Here, alkalinity still exceeded 50 mg/L, the CO 2 concentration remained well below our limit of 20 mg/L (15.4 mg/L) and the pH was near neutral (7.17). Applying the dilution ratio of 0.6 in a full scale treatment plant at the site reduced by 40% the volume of spring water that is directed through each of three parallel reactors that combined react away 49,000 kg of limestone/yr.

Aquacultural Engineering

Efficacy and residual toxicity of a sodium hydroxide based ballast water treatment system for freshwater bulk freighters

The efficacy and residual toxicity of a sodium hydroxide (NaOH) based ballast water treatment system (BWTS) were tested aboard the Great Lakes carrier M/V American Spirit in 1000 L mesocosms containing water from the ship's ballast tanks. NaOH was added to elevate the pH to 11.5 or 11.7 for 48 h, after which pH was reduced to < 9 before discharge by sparging with carbon dioxide to form sodium bicarbonate. In 4 trials, pH 11.7 NaOH BW was highly effective in reducing the densities of organisms relative to uptake water and met the ballast water discharge standards of the US Coast Guard (USCG), the US Environmental Protection Agency vessel general permit (USEPA VGP) and the International Maritime Organization (IMO) G8 for the classes of regulated organisms: ≥ 50 μm, ≥ 10 μm to < 50 μm and indicator bacteria < 10 μm. In addition, densities of heterotrophic bacteria were reduced > 96% in pH 11.7 treated discharge water relative to uptake densities. Seven day whole effluent toxicity tests indicated pH 11.7 NaOH BW met the USEPA VGP daily maximum criteria for residual toxicity. Organism densities in uptake water did not meet the minimum densities for IMO G8 shipboard test validity in 2 of 4 trials for organisms ≥ 10 μm to < 50 μm or in any trials for the < 10 μm size class. The high efficacy and low residual toxicity observed indicates that a NaOH BWTS has great potential for successfully treating large volumes of ballast water released into freshwater systems.

Journal of Great Lakes Research

Application of a fluidized bed reactor charged with aragonite for control of alkalinity, pH and carbon dioxide in marine recirculating aquaculture systems

Control of alkalinity, dissolved carbon dioxide (dCO 2 ), and pH are critical in marine recirculating aquaculture systems (RAS) in order to maintain health and maximize growth. A small-scale prototype aragonite sand filled fluidized bed reactor was tested under varying conditions of alkalinity and dCO 2 to develop and model the response of dCO 2 across the reactor. A large-scale reactor was then incorporated into an operating marine recirculating aquaculture system to observe the reactor as the system moved toward equilibrium. The relationship between alkalinity dCO 2 , and pH across the reactor are described by multiple regression equations. The change in dCO 2 across the small-scale reactor indicated a strong likelihood that an equilibrium alkalinity would be maintained by using a fluidized bed aragonite reactor. The large-scale reactor verified this observation and established equilibrium at an alkalinity of approximately 135 mg/L as CaCO 3 , dCO 2 of 9 mg/L, and a pH of 7.0 within 4 days that was stable during a 14 day test period. The fluidized bed aragonite reactor has the potential to simplify alkalinity and pH control, and aid in dCO 2 control in RAS design and operation. Aragonite sand, purchased in bulk, is less expensive than sodium bicarbonate and could reduce overall operating production costs.

Aquacultural Engineering

Passage of downstream migrant American eels through an airlift-assisted deep bypass

Traditional downstream guidance and bypass facilities for anadromous fishes (i.e., surface bypasses, surface guidance structures, and behavioral barriers) have frequently been ineffective for anguillid eels. Because eels typically spend the majority of their time near the bottom in the vicinity of intake structures, deep bypass structures with entrances near the bottom hold promise for increased effectiveness, thereby aiding in the recovery of this important species. A new design of a deep bypass system that uses airlift technology (the Conte Airlift Bypass) to induce flow in a bypass pipe was tested in a simulated intake entrance environment under controlled laboratory conditions. Water velocities of 0.9&ndash;1.5 m s &minus;1 could be generated at the bypass entrance (opening with 0.073 m 2 area), with corresponding flows through the bypass pipe of 0.07&ndash;0.11 m 3 s &minus;1 . Gas saturation and hydrostatic pressure within the bypass pipe did not vary appreciably from a control (no air) condition under tested airflows. Migratory silver-phase American eels ( Anguilla rostrata ) tested during dark conditions readily located, entered, and passed through the bypass; initial avoidance rates (eels approaching but not entering the bypass entrance) were lower at higher entrance velocities. Eels that investigated the bypass pipe entrance tended to enter headfirst, but those that then exited the pipe upstream did so more frequently at lower entrance velocities. Eels appeared to swim against the flow while being transported downstream through the pipe; median transit times through the bypass for each test velocity ranged from 5.8 to 12.2 s, with transit time decreasing with increasing entrance velocity. Eels did not show strong avoidance of the vertical section of the pipe which contained injected air. No mortality or injury of bypassed eels was observed, and individual eels repeatedly passed through the bypass at rates of up to 40 passes per hour, suggesting that individuals do not avoid repeated entrainment through the bypass. Airlift technology appears to be a viable method for increasing passage effectiveness for American eels through a deep bypass system.

Ecological Engineering

An evaluation of the residual toxicity and chemistry of a sodium hydroxide-based ballast water treatment system for freshwater ships

Nonnative organisms in the ballast water of freshwater ships must be killed to prevent the spread of invasive species. The ideal ballast water treatment system (BWTS) would kill 100% of ballast water organisms with minimal residual toxicity to organisms in receiving waters. In the present study, the residual toxicity and chemistry of a BWTS was evaluated. Sodium hydroxide was added to elevate pH to >11.5 to kill ballast water organisms, then reduced to pH <9 by sparging with wet-scrubbed diesel exhaust (the source of CO 2 ). Cladocerans ( Ceriodaphnia dubia ), amphipods ( Hyalella azteca ), and fathead minnows ( Pimephales promelas ) were exposed for 2 d to BWTS water under an air atmosphere (pH drifted to ≥9) or a 2.5% CO 2 atmosphere (pH 7.5–8.2), then transferred to control water for 5 d to assess potential delayed toxicity. Chemical concentrations in the BWTS water met vessel discharge guidelines with the exception of concentrations of copper. There was little to no residual toxicity to cladocerans or fish, but the BWTS water was toxic to amphipods. Maintaining a neutral pH and diluting BWTS water by 50% eliminated toxicity to the amphipods. The toxicity of BWTS water would likely be minimal because of rapid dilution in the receiving water, with subsurface release likely preventing pH rise. This BWTS has the potential to become a viable method for treating ballast water released into freshwater systems.

Environmental Toxicology and Chemistry

Hydroxide stabilization as a new tool for ballast disinfection: Efficacy of treatment on zooplankton

Effective and economical tools are needed for treating ship ballast to meet new regulatory requirements designed to reduce the introduction of invasive aquatic species from ship traffic. We tested the efficacy of hydroxide stabilization as a ballast disinfection tool in replicated, sequential field trials on board the M/V Ranger III in waters of Lake Superior. Ballast water was introduced into each of four identical 1,320 L stainless steel tanks during a simulated ballasting operation. Two tanks were treated with NaOH to elevate the pH to 11.7 and the remaining two tanks were held as controls without pH alteration. After retention on board for 14&ndash;18 h, CO 2 -rich gas recovered from one of two diesel propulsion engines was sparged into tanks treated with NaOH for 2 h to force conversion of NaOH ultimately to sodium bicarbonate, thereby lowering pH to about 7.1. Prior to gas sparging, the engine exhaust was treated by a unique catalytic converter/wet scrubber process train to remove unwanted combustion byproducts and to provide cooling. The contents of each tank were then drained and filtered through 35-&micro;m mesh plankton nets to collect all zooplankton. The composition and relative survival of zooplankton in each tank were evaluated by microscopy. Zooplankton populations were dominated by rotifers, but copepods and cladocerans were also observed. Hydroxide stabilization was 100% effective in killing all zooplankton present at the start of the tests. Our results suggest hydroxide stabilization has potential to be an effective and practical tool to disinfect ship ballast. Further, using CO 2 released from the ship engine reduces emissions and the neutralized by product, sodium bicarbonate, can have beneficial impacts on the aquatic environment.

Laurentian Great Lakes

Suppressing bullfrog larvae with carbon dioxide

Current management strategies for the control and suppression of the American Bullfrog ( Lithobates catesbeianus = Rana catesbeiana Shaw) and other invasive amphibians have had minimal effect on their abundance and distribution. This study evaluates the effects of carbon dioxide (CO 2 ) on pre- and prometamorphic Bullfrog larvae. Bullfrogs are a model organism for evaluating potential suppression agents because they are a successful invader worldwide. From experimental trials we estimated that the 24-h 50% and 99% lethal concentration (LC 50 and LC 99 ) values for Bullfrog larvae were 371 and 549 mg CO 2 /L, respectively. Overall, larvae that succumbed to experimental conditions had a lower body condition index than those that survived. We also documented sublethal changes in blood chemistry during prolonged exposure to elevated CO 2 . Specifically, blood pH decreased by more than 0.5 pH units after 9 h of exposure and both blood partial pressure of CO 2 (pCO 2 ) and blood glucose increased. These findings suggest that CO 2 treatments can be lethal to Bullfrog larvae under controlled laboratory conditions. We believe this work represents the necessary foundation for further consideration of CO 2 as a potential suppression agent for one of the most harmful invaders to freshwater ecosystems.

Journal of Herpetology

Bactericidal efficacy of elevated pH on fish pathogenic and environmental bacteria

Ship ballast water is a recognized medium for transfer and introductions of nonindigenous species. There is a need for new ballast water treatment methods that effectively and safely eliminate or greatly minimize movements of these species. The present study employed laboratory methods to evaluate the bactericidal efficacy of increased pH (pH 10.0–12.0) for exposure durations of up to 72 h to kill a variety of Gram-negative and Gram-positive bacteria including fish pathogens (Aeromonas spp., Yersinia ruckeri, Edwardsiella ictaluri, Serratia liquefaciens, Carnobacterium sp.), other common aquatic-inhabitant bacteria (Serratia marcescens, Pseudomonas fluorescens, Staphylococcus sp., Bacillus sp.) and indicators listed in International Maritime Organization D2 Standards; namely, Vibrio cholera (an environmental isolate from fish), Escherichia coli and Enterococcus faecalis. Volumes of 5 N NaOH were added to tryptic soy broth to obtain desired pH adjustments. Viable cells were determined after 0, 4, 12, 24, 48, and 72 h. Initial (0 h) cell numbers ranged from 3.40 × 10 4 cfu/mL for Bacillus sp. to 2.44 × 10 7 cfu/mL for E. faecalis. The effective endpoints of pH and treatment duration necessary to realize 100% bactericidal effect varied; however, all bacteria tested were killed within 72 h at pH 12.0 or lower. The lowest parameters examined, 4 h at pH 10.0, were bactericidal to V. cholera, E. ictaluri, three of four isolates of E. coli, and (three of four) Aeromonas salmonicida subsp. salmonicida. Bactericidal effect was attained at pH 10.0 within 12 h for the other A. salmonicida subsp. salmonicida, and within 24 h for P. fluorescens, and the remaining E. coli.

Journal of Advanced Research

Toxicity of elevated partial pressures of carbon dioxide to invasive New Zealand mudsnails

The authors tested the efficacy of elevated partial pressures of CO 2 to kill invasive New Zealand mudsnails. The New Zealand mudsnails were exposed to 100 kPa at three water temperatures, and the survival was modeled versus dose as cumulative &deg;C-h. We estimated an LD50 of 59.4&deg;C-h for adult and juvenile New Zealand mudsnails. The results suggest that CO 2 may be an effective and inexpensive lethal tool to treat substrates, tanks, or materials infested with New Zealand mudsnails.

Environmental Toxicology and Chemistry

Hydrocyclonic separation of invasive New Zealand mudsnails from an aquaculture water source

Invasive New Zealand mudsnails ( Potamopyrgus antipodarum , NZMS) have infested freshwater aquaculture facilities in the western United States and disrupted stocking or fish transportation activities because of the risk of transporting NZMS to naive locations. We tested the efficacy of a gravity-fed, hydrocyclonicseparation system to remove NZMS from an aquaculture water source at two design flows: 367 L/min and 257 L/min. The hydrocyclone effectively filtered all sizes of snails (including newly emerged neonates) from inflows. We modeled cumulative recovery of three sizes of snails, and determined that both juvenile and adult sized snails were transported similarly through the filtration system, but the transit of neonates was faster and similar to the transport of water particles. We found that transit times through the filtration system were different between the two flows regardless of snail size, and the hydrocyclone filter operated more as a plug flow system with dispersion, especially when transporting and removing the larger sized adult and juvenile sized snails. Our study supports hydrocyclonic filtration as an important tool to provide snail free water for aquaculture operations that require uninfested water sources.

Aquaculture

Modeling Carbon Dioxide, pH and Un-Ionized Ammonia Relationships in Serial Reuse Systems

In serial reuse systems, excretion of metabolic carbon dioxide has a significant impact on ambient pH, carbon dioxide, and un-ionized ammonia concentrations. This impact depends strongly on alkalinity, water flow rate, feeding rate, and loss of carbon dioxide to the atmosphere. A reduction in pH from metabolic carbon dioxide can significantly reduce the un-ionized ammonia concentration and increase the carbon dioxide concentrations compared to those parameters computed from influent pH. The ability to accurately predict pH in serial reuse systems is critical to their design and effective operation. A trial and error solution to the alkalinity–pH system was used to estimate important water quality parameters in serial reuse systems. Transfer of oxygen and carbon dioxide across the air–water interface, at overflow weirs, and impacts of substrate-attached algae and suspended bacteria were modeled. Gas transfer at the weirs was much greater than transfer across the air–water boundary. This simulation model can rapidly estimate influent and effluent concentrations of dissolved oxygen, carbon dioxide, and un-ionized ammonia as a function of water temperature, elevation, water flow, and weir type. The accuracy of the estimates strongly depends on assumed pollutional loading rates and gas transfer at the weirs. The current simulation model is based on mean daily loading rates; the impacts of daily variation loading rates are discussed. Copies of the source code and executable program are available free of charge.

Aquacultural Engineering

Factors influencing the nitrification efficiency of fluidized bed filter with a plastic bead medium

The performance of fluidized bed nitrification filters charged with 2 ?? 4 ABS plastic beads (specific gravity 1.06) was evaluated. Three unique bed-height to diameter ratios were established, in triplicate, using column diameters of 12.7, 15.2 and 17.8 cm. Filters received water spiked with recycled nutrients and ammonia (TAN), from one of the three 500 1 feed tank system. With daily ammonia loading fixed at 8.6 g per system, TAN removal increased with column diameter at each of four tests hydraulic loading rates (6, 8, 10 and 12 Lpm). TAN in recirculated water (influent) rose from 0.5 to 1.0 mg/1 as ammonia loading increased from 180 mg/m2-day to 360 mg/m2-day. When hydraulic loading was fixed at 12 Lpm, TAN removal (%) was maximized with ammonia loadings ranging from 225 to 270 mg/m2-day. Biofilm thickness increased with ammonia loading, but decreased with increased hydraulic loading rates. Fluidized beds of ABS plastic beads were effective in reducing energy costs (head loss) of water treatment. ?? 2002 Elsevier Science B.V. All rights reserved.

Aquacultural Engineering

ARD remediation with limestone in a CO2 pressurized reactor

We evaluated a new process for remediation of acid rock drainage (ARD). The process treats ARD with intermittently fluidized beds of granular limestone maintained within a continuous flow reactor pressurized with CO 2 . Tests were performed over a thirty day period at the Toby Creek mine drainage treatment plant, Elk County, Pennsylvania in cooperation with the Pennsylvania Department of Environmental Protection. Equipment performance was established at operating pressures of 0, 34, 82, and 117 kPa using an ARD flow of 227 L/min. The ARD had the following characteristics: pH, 3.1; temperature, 10 &deg;C; dissolved oxygen, 6.4 mg/L; acidity, 260 mg/L; total iron, 21 mg/L; aluminum, 22 mg/L; manganese, 7.5 mg/L; and conductivity, 1400 &mu;S/cm. In all cases tested, processed ARD was net alkaline with mean pH and alkalinities of 6.7 and 59 mg/L at a CO 2 pressure of 0 kPa, 6.6 and 158 mg/L at 34 kPa, 7.4 and 240 mg/L at 82 kPa, and 7.4 and 290 mg/L at 117 kPa. Processed ARD alkalinities were correlated to the settled bed depth (p<0.001) and CO 2 pressure (p<0.001). Iron, aluminum, and manganese removal efficiencies of 96%, 99%, and 5%, respectively, were achieved with filtration following treatment. No indications of metal hydroxide precipitation or armoring of the limestone were observed. The surplus alkalinity established at 82 kPa was successful in treating an equivalent of 1136 L/min (five-fold dilution) of the combined three ARD streams entering the Toby Creek Plant. This side-stream capability provides savings in treatment unit scale as well as flexibility in treatment effect. The capability of the system to handle higher influent acidity was tested by elevating the acidity to 5000 mg/L with sulfuric acid. Net alkaline effluent was produced, indicating applicability of the process to highly acidic ARD.

Conference Paper

Importance of rearing-unit design and stocking density to the behavior, growth and metabolism of lake trout (Salvelinus namaycush)

Juvenile lake trout ( Salvelinus namaycush ) were held at different stocking densities (48 and 96 kg m −3 ) in rearing-units of different design (rectangular plug-flow, circular and cylindrical cross-flow) and the effects on behavior, growth and metabolism were examined. Ambient light levels and current velocities were measured in each of three tank sectors (upstream, middle and downstream) to determine their relative contributions to fish behavior and distribution. Rearing-unit design affected orientation to current, contact with surfaces, agonistic responses, turn rates and avoidance of or contact with other fish. Stocking density primarily affected contact with tank surfaces, avoidance and contact with other fish. Fish in circular tanks distributed themselves uniformly, whereas those in plug-flow tanks crowded upstream and those in cross-flow tanks congregated downstream. Stocking density affected fish distribution most in cross-flow and least in circular tanks. Ambient light, current velocity and total in situ aggression modified fish distribution at low stocking density, but at high density the effects of current velocity and total aggression were undetectable. Growth and food conversion were best in plug-flow, followed by cross-flow and then circular tanks. Net ammonia excretion and oxygen consumption were highest in circular tanks and lowest in cross-flow (NH 3 ) or cross-flow and plug-flow (O 2 ) tanks. Ammonia production was correlated with stocking density, but oxygen consumption was unrelated to density. Results demonstrate the interrelatedness of abiotic and biotic factors in fish culture and the ability to control fish behavior, growth and metabolism through the alteration of several physical and biological variables in the rearing unit.

Aquacultural Engineering

Modeling gas transfer in a spray tower oxygen absorber

A computer model characterizing the performance of a spray tower oxygen absorption system was developed based on finite difference mass transfer calculations. Performance was assessed in terms of oxygen utilization, transfer efficiency, and economy. Pilot scale tests verified model assumptions and performance predictions. Simulation runs indicated spray tower head and oxygen feed requirements for desired changes in dissolved oxygen (DO) exceeded those required for packed column equipment. Spray tower performance was improved by increasing hydraulic loading from 35 to 85 kg m −2 s −1 and by increasing tower height from 1·25 to 2·50 m. The effluent DO concentration that minimized variable costs of oxygen transfer was lower in the spray tower than in the packed tower, indicating clean water use of the spray tower will be limited to moderate effluent DO requirement applications (DO <20 mg l −1 ).

Aquacultural Engineering