Geology topics

Andrea Porras-Alfaro

Publications and source records attributed to Andrea Porras-Alfaro.

3 recordsLinked to original sources

USGS2019

Streptomyces corynorhini sp. nov., isolated from Townsend’s big-eared bats (Corynorhinus townsendii)

Four bacterial strains, with the capability of inhibiting Pseudogymnoascus destructans , the causative agent of white-nose syndrome, were isolated from male Townsend’s big-eared bats ( Corynorhinus townsendii , Family: Vespertilionidae) in New Mexico. Isolates AC161, AC162, AC208, and AC230 T were characterised as a novel clade using morphological, phenotypic and phylogenetic analysis. A draft genome of the type strain was completed to determine its taxonomy and secondary metabolite biosynthetic potential. Multi-locus sequence analysis nests AC230 T with neighbours Streptomyces scopuliridis (NRRL B-24574 T ), Streptomyces lushanensis (NRRL B-24994 T ), Streptomyces odonnellii (NRRL B-24891 T ) and Streptomyces niveus (NRRL 2466 T ). Further phylogenetic analysis showed the MLSA distances between AC230 T and its near neighbours are much greater than the generally accepted threshold (> 0.007) for bacterial species delineation. DNA–DNA relatedness between AC230 T and its near neighbours ranged between 25.7 ± 2.1 and 29.9 ± 2.4%. The DNA G+C content of the genomic DNA of the type strain is 71.7 mol%. Isolate AC230 T presents a white to ivory hue on most ISP media and its micromorphology exhibits ovoid spores with smooth surfaces in flexuous chains. Based on our study of AC230 T , the strain warrants the assignment to a novel species, for which the name Streptomyces corynorhini sp. nov. is proposed. The type strain is AC230 T (= JCM 33171 T , = ATCC TSD155 T ).

Antonie van Leeuwenhoek↗

USGS2017

Skin and fur bacterial diversity and community structure on American southwestern bats: effects of habitat, geography and bat traits

Microorganisms that reside on and in mammals, such as bats, have the potential to influence their host’s health and to provide defenses against invading pathogens. However, we have little understanding of the skin and fur bacterial microbiota on bats, or factors that influence the structure of these communities. The southwestern United States offers excellent sites for the study of external bat bacterial microbiota due to the diversity of bat species, the variety of abiotic and biotic factors that may govern bat bacterial microbiota communities, and the lack of the newly emergent fungal disease in bats, white-nose syndrome (WNS), in the southwest. To test these variables, we used 16S rRNA gene 454 pyrosequencing from swabs of external skin and fur surfaces from 163 bats from 13 species sampled from southeastern New Mexico to northwestern Arizona. Community similarity patterns, random forest models, and generalized linear mixed-effects models show that factors such as location (e.g., cave-caught versus surface-netted) and ecoregion are major contributors to the structure of bacterial communities on bats. Bats caught in caves had a distinct microbial community compared to those that were netted on the surface. Our results provide a first insight into the distribution of skin and fur bat bacteria in the WNS-free environment of New Mexico and Arizona. More importantly, it provides a baseline of bat external microbiota that can be explored for potential natural defenses against pathogens.

PeerJ↗

USGS2017

Western bats as a reservoir of novel Streptomyces species with antifungal activity

At least two-thirds of commercial antibiotics today are derived from Actinobacteria , more specifically from the genus Streptomyces . Antibiotic resistance and new emerging diseases pose great challenges in the field of microbiology. Cave systems, in which actinobacteria are ubiquitous and abundant, represent new opportunities for the discovery of novel bacterial species and the study of their interactions with emergent pathogens. White-nose syndrome is an invasive bat disease caused by the fungus Pseudogymnoascus destructans , which has killed more than six million bats in the last 7 years. In this study, we isolated naturally occurring actinobacteria from white-nose syndrome (WNS)-free bats from five cave systems and surface locations in the vicinity in New Mexico and Arizona, USA. We sequenced the 16S rRNA region and tested 632 isolates from 12 different bat species using a bilayer plate method to evaluate antifungal activity. Thirty-six actinobacteria inhibited or stopped the growth of P. destructans , with 32 (88.9%) actinobacteria belonging to the genus Streptomyces . Isolates in the genera Rhodococcus , Streptosporangium , Luteipulveratus , and Nocardiopsis also showed inhibition. Twenty-five of the isolates with antifungal activity against P. destructans represent 15 novel Streptomyces spp. based on multilocus sequence analysis. Our results suggest that bats in western North America caves possess novel bacterial microbiota with the potential to inhibit P. destructans .

Applied and Environmental Microbiology↗