Geology topics

Amie M.G. Brady

Publications and source records attributed to Amie M.G. Brady.

11 recordsLinked to original sources

USGS2024

The U.S. Geological Survey Ohio Water Microbiology Laboratory

The U.S. Geological Survey Ohio Water Microbiology Laboratory is a part of the Ohio-Kentucky-Indiana Water Science Center. The mission of the laboratory is to provide microbiological data of public health significance from surface waters, groundwaters, and sediments for a variety of study objectives. The laboratory conducts internal projects, works with external cooperators, and assists U.S. Geological Survey offices and National programs. The laboratory offers guidance, study design, and data interpretation expertise to collaborators, all following rigorous quality control and quality assurance procedures.

Fact Sheet↗

USGS2021

Evaluation of a modified rapid viability-polymerase chain reaction method for Bacillus atrophaeus spores in water matrices

A rapid method that provides information on the viability of organisms is needed to protect public health and ensure that remediation efforts following a release of a biological agent are effective. The rapid viability-polymerase chain reaction (RV-PCR) method combines broth culture and molecular methods to provide results on whether viable organisms are present in less than 15 h. In this study, a modified RV-PCR (mRV-PCR) method was compared to a membrane-filtration culture method for the detection of viable Bacillus spores in water matrices. Samples included small and large volumes of chlorine and non‑chlorine treated tap water. Large volume water samples (up to 100 L), were processed by ultrafiltration using a semi-automated waterborne pathogen concentrator, followed by centrifugation as a secondary concentration technique. The concentrated samples were analyzed by mRV-PCR and culture methods. The overall agreement between the mRV-PCR and culture methods when seed concentrations were greater than 10 spores per sample volume analyzed was 96%. The total time from the start of sample processing to the final sample result for the mRV-PCR method was decreased by approximately 2 h, in comparison to the previously published RV-PCR method because of the incorporation of shorter, more efficient primary and secondary concentration steps and a shorter DNA extraction technique. Overall, this study confirmed that RV-PCR is a promising approach for identifying viable Bacillus spores in small- and large-volume water samples and for producing results in less time than traditional culture methods.

Journal of Microbiological Methods↗

USGS2020

Predicting microcystin concentration action-level exceedances resulting from cyanobacterial blooms in selected lake sites in Ohio

Cyanobacterial harmful algal blooms and the toxins they produce are a global water-quality problem. Monitoring and prediction tools are needed to quickly predict cyanotoxin action-level exceedances in recreational and drinking waters used by the public. To address this need, data were collected at eight locations in Ohio, USA, to identify factors significantly related to observed concentrations of microcystins (a freshwater cyanotoxin) that could be used in two types of site-specific regression models. Real-time models include easily- or continuously-measured factors that do not require that a sample be collected; comprehensive models use a combination of discrete sample-based measurements and real-time factors. The study sites included two recreational sites and six water treatment plant sites. Real-time models commonly included variables such as phycocyanin, pH, specific conductance, and streamflow or gage height. Many real-time factors were averages over time periods antecedent to the time the microcystin sample was collected, including water-quality data compiled from continuous monitors. Comprehensive models were useful at some sites with lagged variables for cyanobacterial toxin genes, dissolved nutrients, and (or) nitrogen to phosphorus ratios. Because models can be used for management decisions, important measures of model performance were sensitivity, specificity, and accuracy of estimates above or below the microcystin concentration threshold standard or action level. Sensitivity is how well the predictive tool correctly predicts exceedance of a threshold, an important measure for water-resource managers. Sensitivities >90% at four Lake Erie water treatment plants indicated that models with continuous monitor data were especially promising. The planned next steps are to collect more data to build larger site-specific datasets and validate models before they can be used for management decisions.

Ohio↗

USGS2020

Pilot-scale testing of dairy manure treatments to reduce nutrient transport from land application, northwest Ohio, 2015–17

Manure and wastewater from large livestock operations have the potential to negatively affect surface water and groundwater, including the eutrophication of surface waters and harmful algal blooms. In the Western Lake Erie Basin, where there is a high density of animal agriculture, harmful algal blooms have been attributed, in part, to phosphorus loading from dairy manure and fertilizer applications. Liquid lagoon manure produced by dairy operations typically has low nutrient concentrations and high-water content, so transportation costs are high relative to the value of the nutrients when applied to fields. Treatment systems are needed to transform manure into a dewatered product that is more economical to transport greater distances and that slows and (or) reduces the release of nutrients in soil, allowing nutrients to remain available for crop growth. This study was designed to pilot test a treatment solution in the Western Lake Erie Basin. The U.S. Geological Survey and Bowling Green State University field tested a dewatering treatment process (coagulant/polymer mixture) for dairy manure at pilot-scale test plots at The Ohio State University Agricultural Research and Development Center Northwest Agricultural Research Station. Automatic samplers were used to collect samples during 13 baseline and 9 post-manure application rainfall events that resulted in substantial surface runoff and (or) tile flow from October 2015 through early November 2017. Results are reported for three test plots that received liquid lagoon manure (raw manure) and three test plots that received polymer-treated manure (treated manure). Nutrient concentrations and flow volumes in surface runoff and tile flow were determined in baseline and post-manure application rainfall events. Nutrient concentration ranges are reported for 9 baseline and 9 post-manure application events as follows: dissolved reactive phosphorus, less than (<) 0.013−2.16 milligrams per liter (mg/L); nitrate plus nitrite, filtered, 0.32−77 mg/L; ammonia, filtered, <0.05−2.6 mg/L; total phosphorus, <0.01−12.8 mg/L; and total nitrogen, 1.49−77.2 mg/L. Volumes are reported for 6 baseline and 9 post-manure application rainfall events. None of the post-manure application runoff volumes were significantly different by plot or by treatment type (raw manure versus treated manure). Because concentrations alone do not reflect the true effects of different manure treatments, loads and flow-weighted mean concentrations of nutrients during post-manure application rainfall events were compared between plots with treated manure and those with raw manure. Loads of dissolved reactive phosphorus, total phosphorus, nitrate plus nitrite, and total nitrogen were calculated using the U.S. Geological Survey Graphical Constituent Loading and Analysis System. Loads of ammonia were not calculated because many of the ammonia concentrations were below the reporting limit. During the post-manure application period, higher nitrogen loads resulted from tile flow than surface runoff. For phosphorus, the opposite was true in that higher loads resulted from surface runoff than tile flow. Combined loads (surface runoff and tile flow) of dissolved reactive phosphorus were significantly different between raw manure and treated manure plots, but there was no significant difference in combined loads of total phosphorus, nitrate plus nitrite, or total nitrogen between raw manure and treated manure plots. Flow-weighted mean concentrations were calculated for the combined loads for the post-manure application rainfall events. Flow-weighted mean concentrations of dissolved reactive phosphorus and, to a lesser extent, total phosphorus were significantly different between raw manure and treated manure plots. Flow-weighted mean concentrations of nitrate plus nitrite and total nitrogen were not significantly different between raw manure and treated manure plots. The differences in loads and flow-weighted mean concentrations between raw manure and treated manure plots indicate that dissolved reactive phosphorus was likely retained in the soil and hydrological transport was reduced for the plots amended with the treated manure as compared to raw manure. Although confirmation field testing needs to be done, these results indicate that the use of this coagulant/polymer mixture shows potential in helping to reduce flow of dissolved phosphorus from agricultural fields with applied manure.

Ohio↗

USGS2020

Nowcasting methods for determining microbiological water quality at recreational beaches and drinking-water source waters

Nowcasts are tools used to provide timely and accurate water-quality assessments of threats to drinking-water and recreational resources from fecal contamination or cyanobacterial harmful algal blooms. They use mathematical models and techniques to provide near-real-time estimates of fecal-indicator bacteria (FIB) and cyanotoxin concentrations. Techniques include logic-based thresholds, decision trees (built with machine learning), multiple linear and binary logistic regression, artificial neural networks, and process-based deterministic models. The type of site (freshwater, marine, or river) and dependent variable (FIB or cyanotoxin) dictate which explanatory variables are used in models. Nowcast systems notify the public of associated public-health risks and can also be used to manage data for FIB models; work is ongoing to incorporate cyanotoxin models into some nowcasts. The Great Lakes NowCast in the USA has been operational since 2010 and includes 25 lake beaches and one recreational river site. Examples of other operational FIB nowcasts are described for locations in the USA and around the world. In many cases, models predicted exceedances of FIB standards with accuracies as good as or better than using the previous measured FIB concentration (persistence method). Accuracy and timeliness are vital to beach management decisions that protect public health and support the local recreation-driven economy. Nowcasts benefit the public by providing estimates of water-quality conditions in near-real-time. Managers can use nowcasts at recreational and drinking-water treatment plant sites when FIB or cyanotoxins are projected to be elevated to target sample collection, to provide near-real-time recreational advisories to the public, or to preemptively optimize drinking-water treatments or change intake options to mitigate possible adverse effects on drinking-water quality.

Journal of Microbiological Methods↗

USGS2019

Real-time assessments of water quality—A nowcast for Escherichia coli and cyanobacterial toxins

Threats to our recreational and drinking waters include disease-causing (pathogenic) organisms from fecal contamination and toxins produced by some species of cyanobacteria (cyanotoxins) that can cause acute and (or) chronic illnesses. Because traditional laboratory methods for detecting these threats take too long for prompt public health protection, tools for real-time assessments are needed to protect public health. To address this need, the U.S. Geological Survey is collaborating with State and local partners to develop models that provide real-time estimates of Escherichia coli ( E . coli ) (for pathogens) and (or) microcystin (for freshwater cyanotoxins) levels at inland and Great Lakes beaches and drinking-water intakes. Model results are then used to inform the public of water-quality conditions in near-real time through the Great Lakes NowCast ( https://ny.water.usgs.gov/maps/nowcast/ ). Behind the scenes, the NowCast provides speed and efficiency for managers by automating data management and standardizing methods among agencies.

Fact Sheet↗

USGS2019

Escherichia coli and microbial source tracking marker concentrations in and near a constructed wetland in Maumee Bay State Park, Oregon, Ohio, 2015–16

Elevated Escherichia coli ( E. coli ) concentrations at the Maumee Bay State Park (MBSP) Lake Erie beach have resulted in frequent recreational water-quality advisories. After the construction of a wetland along Berger Ditch in Maumee Bay State Park, Oregon, Ohio, samples were collected and analyzed for concentrations of E. coli and microbial source tracking (MST) markers. This study was done by the U.S. Geological Survey, in cooperation with the University of Toledo, to provide data that can be used to help evaluate the effects of the wetland on water quality in MBSP. From July 2015 to February 2016, 116 samples were collected from six sites. Median GenBac (general marker) and E. coli concentrations were higher in samples from Berger Ditch sites than in samples from the wetland sites. No statistically significant difference was found between median E. coli concentrations in samples collected at Berger Ditch sites upstream and downstream from the wetland. The frequency of detection of the human-associated Bacteroides MST marker (HF183) decreased from 39 percent upstream from the wetland to 22 percent downstream from the wetland; however, the HF183 median concentrations from these two groups of samples were nearly the same (2,700 to 2,800 copies per 100 milliliters from upstream to downstream). The waterfowl-associated Helicobacter MST marker (GFD) was detected in 13 percent of samples from the Berger Ditch site upstream from the wetland, although it was not detected in samples from the Berger Ditch site downstream from the wetland. The ruminant-associated MST marker, Rum2Bac, was not detected at any site during this study.

Ohio↗

USGS2017

Multi-year microbial source tracking study characterizing fecal contamination in an urban watershed

Microbiological and hydrological data were used to rank tributary stream contributions of bacteria to the Little Blue River in Independence, Missouri. Concentrations, loadings and yields of E. coli and microbial source tracking (MST) markers, were characterized during base flow and storm events in five subbasins within Independence, as well as sources entering and leaving the city through the river. The E. coli water quality threshold was exceeded in 29% of base-flow and 89% of storm-event samples. The total contribution of E. coli and MST markers from tributaries within Independence to the Little Blue River, regardless of streamflow, did not significantly increase the median concentrations leaving the city. Daily loads and yields of E. coli and MST markers were used to rank the subbasins according to their contribution of each constituent to the river. The ranking methodology used in this study may prove useful in prioritizing remediation in the different subbasins.

Missouri↗

USGS2016

Estimating microcystin levels at recreational sites in western Lake Erie and Ohio

Cyanobacterial harmful algal blooms (cyanoHABs) and associated toxins, such as microcystin, are a major global water-quality issue. Water-resource managers need tools to quickly predict when and where toxin-producing cyanoHABs will occur. This could be done by using site-specific models that estimate the potential for elevated toxin concentrations that cause public health concerns. With this study, samples were collected at three Ohio lakes to identify environmental and water-quality factors to develop linear-regression models to estimate microcystin levels. Measures of the algal community (phycocyanin, cyanobacterial biovolume, and cyanobacterial gene concentrations) and pH were most strongly correlated with microcystin concentrations. Cyanobacterial genes were quantified for general cyanobacteria, general Microcystis and Dolichospermum , and for microcystin synthetase ( mcyE ) for Microcystis , Dolichospermum , and Planktothrix. For phycocyanin, the relations were different between sites and were different between hand-held measurements on-site and nearby continuous monitor measurements for the same site. Continuous measurements of parameters such as phycocyanin, pH, and temperature over multiple days showed the highest correlations to microcystin concentrations. The development of models with high R 2 values (0.81–0.90), sensitivities (92%), and specificities (100%) for estimating microcystin concentrations above or below the Ohio Recreational Public Health Advisory level of 6 μg L −1 was demonstrated for one site; these statistics may change as more data are collected in subsequent years. This study showed that models could be developed for estimates of exceeding a microcystin threshold concentration at a recreational freshwater lake site, with potential to expand their use to provide relevant public health information to water resource managers and the public for both recreational and drinking waters.

Ohio↗

USGS2015

Water Quality, Cyanobacteria, and Environmental Factors and Their Relations to Microcystin Concentrations for Use in Predictive Models at Ohio Lake Erie and Inland Lake Recreational Sites, 2013-14

Harmful cyanobacterial “algal” blooms (cyanoHABs) and associated toxins, such as microcystin, are a major water-quality issue for Lake Erie and inland lakes in Ohio. Predicting when and where a bloom may occur is important to protect the public that uses and consumes a water resource; however, predictions are complicated and likely site specific because of the many factors affecting toxin production. Monitoring for a variety of environmental and water-quality factors, for concentrations of cyanobacteria by molecular methods, and for algal pigments such as chlorophyll and phycocyanin by using optical sensors may provide data that can be used to predict the occurrence of cyanoHABs. To test these monitoring approaches, water-quality samples were collected at Ohio recreational sites during May–November in 2013 and 2014. In 2013, samples were collected monthly at eight sites at eight lakes to facilitate an initial assessment and select sites for more intensive sampling during 2014. In 2014, samples were collected approximately weekly at five sites at three lakes. Physical water-quality parameters were measured at the time of sampling. Composite samples were preserved and analyzed for dissolved and total nutrients, toxins, phytoplankton abundance and biovolume, and cyanobacterial genes by molecular methods. Molecular assays were done to enumerate (1) general cyanobacteria, (2) general Microcystis and Dolichospermum ( Anabaena ), (3) mcyE genes for Microcystis , Dolichospermum ( Anabaena ), and Planktothrix targeting deoxyribonucleic acid (DNA), and (4) mcyE transcripts for Microcystis , Dolichospermum ( Anabaena ), and Planktothrix targeting ribonucleic acid (RNA).The DNA assays for the mcyE gene provide data on cyanobacteria that have the potential to produce microcystin, whereas the RNA assays provide data on cyanobacteria that are actively transcribing the toxin gene. Environmental data were obtained from available online sources. Quality-control (QC) samples were collected and analyzed for all constituents to characterize bias and variability; however, QC data for molecular assays were examined in more detail than for the other constituents. The QC data for molecular assays suggested that sampling variability and qPCR variability were small in comparison with the combined variability associated with sample filtering, extraction and purification, and the matrix itself. A total of 46 water-quality samples were collected during 2013 at 8 beach sites—Buck Creek, Buckeye Crystal, Deer Creek, Harsha Main, Maumee Bay State Park (MBSP) Inland (negative control site), MBSP Lake Erie, Port Clinton, and Sandusky Bay. Microcystin was detected in 67–100 percent of samples at all sites except for MBSP Inland, where microcystin was detected in only 20 percent of samples. Microcystin concentrations ranged from <0.10 to 48 micrograms per liter (µ/L), with the widest range found at MBSP Lake Erie and the highest concentrations found at Buckeye Crystal. Saxitoxin was detected in five samples, and cylindrospermopsin was not detected in any samples. A total of 65 water-quality samples were collected during 2014 at 5 sites on 3 lakes—Buckeye Fairfield and Onion Island, Harsha Main and Campers, and MBSP Lake Erie beach. Four of the sites were bathing beaches and one site, Onion Island, was an offshore boater swim area. Concentrations of microcystin ranged from <0.10 to 240 µ/L and, as in 2013, the widest range was found at MBSP Lake Erie. At Buckeye Lake, microcystin concentrations were consistently high (greater than 20 µ/L), ranging from 23 to 81 µ/L. At Harsha Main and Campers, microcystin concentrations ranged from <0.10 to 15 µ/L. Saxitoxin was detected in four samples collected at MBSP Lake Erie. Throughout the 2014 season, the cyanobacterial community, as determined by molecular and microscopy methods, and the dominance associated with the highest microcystin concentrations were unique to individual lakes. At Buckeye Lake, Planktothrix dominated the cyanobacterial community throughout the season and Planktothrix DNA and RNA were found in 100 percent of samples; Microcystis mcyE DNA was found in low concentrations. At Harsha Lake, Dolichospermum and Microcystis were a substantial percentage of the community from late May through August, and the highest microcystin concentrations occurred in June and July. At MBSP Lake Erie, Microcystis generally dominated from mid-July through early November, and the highest microcystin concentrations occurred in August. Spearman’s correlation coefficient (rho) was computed to determine the relations between environmental and water-quality factors and microcystin concentrations at four sites—Buckeye Fairfield, Buckeye Onion Island, Harsha Main, and MBSP Lake Erie. Factors were evaluated for use as potential independent variables in two types of predictive models—daily and long-term models. Easily or continuously measured water-quality factors and available environmental data are used for daily predictions that do not require a site visit. Data from factors used in daily predictions and results from samples collected and analyzed in a laboratory are used for long-term predictions (a few days to several weeks). A few statistically significant correlations (p ≤ 0.05) between microcystin concentrations and factors for both daily and long-term predictions were found at Buckeye Onion Island, and many were found at Harsha Main and MBSP Lake Erie. There were only a few statistically significant factors for daily predictions at Buckeye Fairfield, likely because of the lack of variability in microcystin concentrations. Among factors for daily predictions, phycocyanin had the highest Spearman’s correlation to microcystin concentrations (rho = 0.79 to 0.93) at all sites except for Buckeye Fairfield. Turbidity, pH, algae category, and Secchi depth were significantly correlated to microcystin concentrations at Harsha Main and MBSP Lake Erie. Algae categories were observational categories from 0 (none) to 4 (extreme). Several discharge variables (Maumee River at Waterville, river mouth is approximately 3.5 miles from the beach) at MBSP Lake Erie were promising environmental factors for daily predictions. In addition to discrete water-quality measurements recorded at Harsha Main at the time of sampling, many manipulated measurements (factors derived from mathematical manipulation of time-series data) available from a nearby continuous monitor were strongly correlated to microcystin concentrations; the highest correlation was found for the relation between microcystin concentrations and the antecedent 7-day average phycocyanin (rho = 0.98). For long-term predictions, the most highly correlated molecular assays were Planktothrix mcyE DNA at Buckeye Onion Island and Microcystis mcyE DNA at Harsha Main and MBSP Lake Erie. Concentrations of several nutrient constituents were significantly correlated to microcystin concentrations including total nitrogen at Buckeye Onion Island, ammonia and nitrate plus nitrite (both negatively correlated) at Harsha Main and MBSP Lake Erie, and total phosphorus at MBSP Lake Erie. The results of this study showed that water-quality and environmental variables are promising for use in site-specific daily or long-term predictive models. In order to develop more accurate models to predict toxin concentrations at freshwater lake sites, data need to be collected more frequently and for consecutive days in future studies.

Ohio↗

USGS2015

Towards automating measurements and predictions of Escherichia coli concentrations in the Cuyahoga River, Cuyahoga Valley National Park, Ohio, 2012–14

Nowcasts are systems that can provide estimates of the current bacterial water-quality conditions based on predictive models using easily-measured, explanatory variables; nowcasts can provide the public with the information to make informed decisions on the risk associated with recreational activities in natural water bodies. Previous studies on the Cuyahoga River within Cuyahoga Valley National Park (CVNP) have found that predictive models can be used to provide accurate assessments of the recreational water quality. However, in order to run the previously developed nowcasts for CVNP, manual collection and processing of samples is required on a daily basis to acquire the required explanatory variable data (laboratory-measured turbidity). The U.S. Geological Survey and the National Park Service collaborated to develop a more automated approach to provide more timely results to park visitors regarding the recreational water quality of the river. In May 2012, an in-stream water-quality sensor was installed by the U.S. Geological Survey at Jaite, Ohio (a site centrally located in CVNP on the Cuyahoga River), to provide near-real-time measurements of turbidity and water temperature. To transition from methods used during previous studies at CVNP, a relation between laboratory- and in-stream measured turbidity was developed after the recreational season of 2012. During the recreational seasons of 2012 through 2014, discrete water samples were collected and processed to determine Escherichia coli ( E. coli ) concentrations at Jaite and one site upstream of Jaite (Lock 29) within CVNP. Predictive models, using in-stream turbidity measurements, were developed for the recreational seasons of 2013 and 2014 to estimate recreational water quality in regards to Ohio’s single-sample water-quality standard for primary-contact recreation. A computer program was developed to manage the nowcasts by running the predictive models and posting the results to a publicly accessible Web site daily by 9 a.m. The nowcasts were able to correctly predict E. coli concentrations above or below the water-quality standard at Jaite for 79 percent of the samples compared with the measured concentrations. In comparison, the persistence model (using the previous day’s sample concentration) correctly predicted concentrations above or below the water-quality standard in only 68 percent of the samples. To determine if the Jaite nowcast could be used for the stretch of the river between Lock 29 and Jaite, the model predictions for Jaite were compared with the measured concentrations at Lock 29. The Jaite nowcast provided correct responses for 77 percent of the Lock 29 samples, which was a greater percentage than the percentage of correct responses (58 percent) from the persistence model at Lock 29.

Ohio↗